##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240589
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157359646534132	31.0	31.0	33.0	28.0	34.0
2	31.05885971511582	31.0	31.0	34.0	28.0	34.0
3	30.965397420497197	31.0	31.0	34.0	28.0	34.0
4	34.82657145588535	35.0	35.0	37.0	32.0	37.0
5	34.56926958422871	35.0	35.0	37.0	32.0	37.0
6	34.77707625868182	35.0	35.0	37.0	32.0	37.0
7	35.229648903316445	36.0	35.0	37.0	33.0	37.0
8	34.44515335281331	36.0	35.0	37.0	30.0	37.0
9	36.35154142541845	39.0	35.0	39.0	32.0	39.0
10	36.233385566256146	37.0	35.0	39.0	32.0	39.0
11	36.606145750636976	38.0	35.0	39.0	32.0	39.0
12	36.668575870052244	39.0	35.0	39.0	33.0	39.0
13	36.61439633565957	39.0	35.0	39.0	32.0	39.0
14	37.57949449060431	39.0	36.0	41.0	32.0	41.0
15	37.58838517139188	39.0	36.0	41.0	32.0	41.0
16	37.82012893357552	39.0	36.0	41.0	33.0	41.0
17	37.51701449359697	39.0	36.0	41.0	32.0	41.0
18	37.54851219299303	39.0	36.0	40.0	33.0	41.0
19	37.452231814422106	39.0	36.0	40.0	32.0	41.0
20	37.22141078769187	39.0	35.0	40.0	32.0	41.0
21	37.23781635901891	39.0	35.0	40.0	32.0	41.0
22	37.380628374530836	39.0	35.0	41.0	33.0	41.0
23	37.39826010332975	39.0	35.0	41.0	33.0	41.0
24	37.28826338693789	39.0	35.0	41.0	32.0	41.0
25	37.01309286791998	39.0	35.0	40.0	32.0	41.0
26	37.079995344758075	39.0	35.0	40.0	32.0	41.0
27	37.057500550731746	39.0	35.0	40.0	32.0	41.0
28	36.88245098487462	39.0	35.0	41.0	31.0	41.0
29	36.930483106043916	39.0	35.0	41.0	31.0	41.0
30	36.651517733562216	39.0	35.0	40.0	31.0	41.0
31	36.613573355390315	39.0	35.0	40.0	31.0	41.0
32	36.426241432484446	39.0	35.0	40.0	30.0	41.0
33	36.31949507250955	39.0	35.0	41.0	30.0	41.0
34	36.09766448175104	39.0	35.0	41.0	29.0	41.0
35	36.02658060011056	39.0	35.0	41.0	29.0	41.0
36	36.0451516902269	39.0	35.0	40.0	29.0	41.0
37	36.008017822926234	39.0	35.0	41.0	29.0	41.0
38	35.822892983469735	39.0	35.0	40.0	28.0	41.0
39	35.848064541604145	39.0	35.0	40.0	28.0	41.0
40	35.86062122540931	39.0	35.0	40.0	28.0	41.0
41	35.48069945009955	38.0	35.0	40.0	26.0	41.0
42	35.68591664623071	39.0	35.0	40.0	27.0	41.0
43	35.72987543071379	39.0	35.0	40.0	28.0	41.0
44	35.851950837320075	39.0	35.0	40.0	28.0	41.0
45	35.873955168357654	39.0	35.0	40.0	28.0	41.0
46	35.743454605156515	39.0	35.0	40.0	28.0	41.0
47	35.526723998187784	38.0	35.0	40.0	27.0	41.0
48	35.573866635631724	38.0	35.0	40.0	27.0	41.0
49	35.67783231984837	38.0	35.0	40.0	28.0	41.0
50	35.57845121763672	38.0	35.0	40.0	28.0	41.0
51	34.76562103836834	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	12.0
10	16.0
11	21.0
12	8.0
13	12.0
14	14.0
15	19.0
16	24.0
17	54.0
18	76.0
19	195.0
20	395.0
21	757.0
22	1227.0
23	1713.0
24	1987.0
25	2697.0
26	3133.0
27	3291.0
28	3325.0
29	3643.0
30	4430.0
31	5625.0
32	7428.0
33	10411.0
34	17583.0
35	19849.0
36	20251.0
37	30684.0
38	51256.0
39	50441.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.76652299149172	18.161678214714723	19.06030616528603	15.01149262850754
2	29.841347692537894	34.33033097938809	19.45974254849557	16.368578779578453
3	28.09313809027013	32.04510596910083	25.409307989974607	14.452447950654435
4	26.281334558105318	21.495995245002888	35.59763746472199	16.6250327321698
5	31.945766431549238	25.84407433423806	23.97075510517937	18.239404129033332
6	23.346038264426056	38.57242018546152	25.028991350394243	13.05255019971819
7	64.84585745815478	6.483671323294082	23.286600800535354	5.383870418015786
8	61.70232221755775	16.238065746979288	14.974915727651721	7.084696307811246
9	56.00214473645927	9.466351329445652	16.141635735632136	18.38986819846294
10	40.04879691091446	21.227071894392513	23.345206971224787	15.378924223468237
11	33.49072484610684	22.350980302507594	26.28756925711483	17.870725594270727
12	27.439741634073044	19.8845333743438	29.633940038821393	23.041784952761766
13	25.430505966606955	23.14444966311843	34.21020911180478	17.214835258469837
14	18.916492441466566	30.582861228069447	28.248174272306713	22.25247205815727
15	16.680729376654792	24.925495346836307	38.3467240813171	20.047051195191802
16	18.453877774960617	25.729771519063632	29.613989001990948	26.202361703984806
17	18.488376442813262	28.77479851531034	31.672270968331883	21.06455407354451
18	17.89441745050688	26.933484074500498	31.712173041992774	23.459925432999846
19	20.664702043734334	26.45258095756664	28.454334986221312	24.428382012477712
20	23.08334961282519	27.24979113758318	33.634538569926306	16.03232067966532
21	21.234969179804565	32.239628578197674	29.262767624454984	17.26263461754278
22	18.578987401751533	24.294128160472837	32.3406307021518	24.786253735623824
23	21.648121900835037	28.369958726292555	30.623594594931607	19.3583247779408
24	22.103254928529566	25.23473641770821	29.15428386168944	23.507724792072786
25	18.25810822606187	32.5588451674848	26.984192959777882	22.198853646675452
26	17.553587237986772	27.258104069595866	31.471098013624893	23.717210678792462
27	22.311078228846707	29.825553121713792	26.969229682155042	20.894138967284455
28	17.096791623889704	29.46560316556451	31.926646687920062	21.51095852262572
29	20.379152829098587	27.28969321124407	28.82716998699026	23.50398397266708
30	18.48338868360565	32.927939348848035	27.69785817306693	20.89081379447938
31	24.586327720718735	26.571040238747408	24.294543807073474	24.548088233460383
32	23.965767345971763	30.280686149408325	23.255842952088415	22.497703552531494
33	21.391667948243686	26.62756817643367	24.6295549671847	27.35120890813795
34	20.923234229328855	30.66391231519313	25.417205275386657	22.99564818009136
35	19.907809583979315	28.573209914002717	24.321560836114703	27.19741966590326
36	21.175947362514496	35.590571472511215	22.848509283466825	20.384971881507465
37	18.60974525019847	31.579997422991074	27.35952184015063	22.450735486659823
38	22.281567320201674	26.954682051132846	22.609096841501483	28.154653787164
39	21.56623952051008	29.28396560108733	24.893490558587466	24.25630431981512
40	20.50966586169775	25.07970023567162	26.977126967567095	27.433506935063534
41	17.991263108454667	32.59500642173998	24.12828516681976	25.285445302985586
42	18.48712950301136	26.137520834285855	29.505920885826036	25.869428776876745
43	21.712547123933344	28.759835237687508	25.093832220093187	24.433785418285954
44	20.855068186824834	25.011534193167602	28.872475466459395	25.260922153548165
45	19.540793635619252	24.19229474331744	27.070647452709807	29.196264168353498
46	24.040583734085928	29.042059279518185	24.559726338278143	22.357630648117745
47	17.14791615576772	25.68321909979259	34.42011064512509	22.7487540993146
48	19.969740927473826	27.054021588684435	27.202407425110874	25.773830058730862
49	18.968863913146485	23.038459779956693	33.94793610680455	24.044740200092274
50	20.799787188940474	24.63412707979168	29.025017768892177	25.541067962375667
51	18.923974080277983	23.79576788631234	26.200699117582264	31.079558915827405
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	251.0
2	424.0
3	2182.0
4	3940.0
5	2656.5
6	1373.0
7	1294.5
8	1216.0
9	1137.5
10	1059.0
11	1040.5
12	1022.0
13	1011.5
14	1001.0
15	908.0
16	815.0
17	810.5
18	806.0
19	815.5
20	825.0
21	980.5
22	1136.0
23	1112.0
24	1088.0
25	1445.5
26	2067.0
27	2331.0
28	3107.5
29	3884.0
30	4156.0
31	4428.0
32	4760.5
33	5093.0
34	5496.0
35	5899.0
36	5936.0
37	5973.0
38	6614.5
39	7256.0
40	8233.0
41	9210.0
42	10344.5
43	11479.0
44	12991.5
45	14504.0
46	26494.0
47	38484.0
48	30755.0
49	23026.0
50	23076.5
51	23127.0
52	19105.0
53	15083.0
54	13470.0
55	11857.0
56	10434.5
57	9012.0
58	8413.0
59	7814.0
60	7497.5
61	7181.0
62	6786.5
63	6392.0
64	5363.0
65	4334.0
66	3483.0
67	2632.0
68	2389.5
69	2147.0
70	1684.5
71	1222.0
72	900.0
73	578.0
74	533.5
75	356.5
76	224.0
77	167.5
78	111.0
79	116.0
80	121.0
81	73.0
82	25.0
83	28.5
84	32.0
85	36.5
86	41.0
87	27.0
88	13.0
89	7.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.71558965704999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50544898663642	25.457107349047547
2	9.41491197790818	6.348586178087942
3	3.3162384732974997	3.354268067118613
4	1.6445584101780166	2.2178902609845004
5	1.124315794664431	1.895348498892302
6	0.8900833374426746	1.8005810739476866
7	0.750776665516051	1.7719014585039217
8	0.6250308200601608	1.6858626121726263
9	0.5621578973322156	1.7058136490030715
>10	6.016075743379852	37.00169168166458
>50	0.0875289708565511	1.998013209248968
>100	0.04807929385078159	3.0175943206048492
>500	0.00493120962572119	1.3358881744385653
>1k	0.007396814438581784	5.52519026223144
>5k	0.002465604812860595	4.8842632040533855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGC	6317	2.625639576206726	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCG	5434	2.2586236278466596	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTC	4874	2.0258615314914645	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	2361	0.9813416240975272	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2333	0.9697035192797675	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCCACAT	1494	0.6209760213476094	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCT	1217	0.5058419129719147	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTA	1014	0.4214656530431566	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTC	890	0.36992547456450625	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	876	0.3641064221556264	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	789	0.32794516790044437	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	659	0.2739111098179884	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC	451	0.18745661688605877	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	321	0.13342255880360282	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	320	0.13300691220296856	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGCCACA	306	0.12718785979408867	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATG	295	0.12261574718711163	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	292	0.12136880738520879	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	265	0.11014634916808332	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	262	0.10889940936618049	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTAT	253	0.105158589960472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.288458740840188	0.0
2	0.0	0.0	0.0	1.8899450930840562	0.0
3	0.0	0.0	0.0	2.303097814114527	0.0
4	0.0	0.0	0.0	3.1959067122769538	0.0
5	0.0	0.0	0.0	6.573035342430452	0.0
6	0.0	0.0	0.0	7.348216252613378	0.0
7	0.0	0.0	0.0	8.538628116829946	0.0
8	0.0	0.0	0.0	9.869944178661536	0.0
9	0.0	0.0	0.0	10.249429525040629	0.0
10	0.0	0.0	0.0	13.417487915075087	0.0
11	0.0	0.0	0.0	14.816138726209427	0.0
12	0.0	0.0	0.0	18.573583995943288	0.0
13	0.0	0.0	0.0	19.15881440963635	0.0
14	0.0	0.0	0.0	19.47761535232284	0.0
15	0.0	0.0	0.0	20.315143252600908	0.0
16	0.0	0.0	0.0	21.120666364630136	0.0
17	0.0	0.0	0.0	21.87714317778452	0.0
18	0.0	0.0	0.0	22.626138352127487	0.0
19	0.0	0.0	0.0	23.84813935799226	0.0
20	0.0	0.0	0.0	24.50402969379315	0.0
21	0.0	0.0	0.0	25.12874653454647	0.0
22	0.0	0.0	0.0	25.81497907219366	0.0
23	0.0	0.0	0.0	26.409353711100675	0.0
24	0.0	0.0	0.0	26.886931655229457	0.0
25	0.0	0.0	0.0	27.330842224706867	0.0
26	0.0	0.0	0.0	27.75604869715573	0.0
27	0.0	0.0	0.0	28.211597371450896	0.0
28	0.0	0.0	0.0	28.64636371571435	0.0
29	0.0	0.0	0.0	29.063672902751165	0.0
30	0.0	0.0	0.0	29.49178890140447	0.0
31	0.0	0.0	0.0	29.953987921309785	0.0
32	0.0	0.0	0.0	30.383766506365628	0.0
33	0.0	0.0	0.0	30.792347114789123	0.0
34	0.0	0.0	0.0	31.2237882862475	0.0
35	0.0	0.0	0.0	31.652735578102074	0.0
36	0.0	0.0	0.0	32.093736621375044	0.0
37	0.0	0.0	0.0	32.52102132682708	0.0
38	0.0	0.0	0.0	32.9420713332696	0.0
39	0.0	0.0	0.0	33.38847578235082	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCC	25	3.8815815E-5	45.000004	37
CGACAGG	25	3.8815815E-5	45.000004	2
ATCACCC	25	3.8815815E-5	45.000004	36
TAGCACG	25	3.8815815E-5	45.000004	1
TCGTCCC	25	3.8815815E-5	45.000004	38
AACTGCA	25	3.8815815E-5	45.000004	17
CACCGAG	50	2.1827873E-11	45.000004	16
GCACTAG	25	3.8815815E-5	45.000004	1
CTTCGGG	20	7.022156E-4	45.0	25
GATACGC	20	7.022156E-4	45.0	27
ACACGCG	35	1.206572E-7	45.0	36
CACGGTT	20	7.022156E-4	45.0	7
CGGTTTA	20	7.022156E-4	45.0	9
TCGGGTA	20	7.022156E-4	45.0	27
CGCCTAA	20	7.022156E-4	45.0	28
GTGCTCA	20	7.022156E-4	45.0	8
AGGCACG	20	7.022156E-4	45.0	1
TTGCACT	20	7.022156E-4	45.0	34
CGGGTGA	30	2.1583237E-6	44.999996	6
GATCACC	30	2.1583237E-6	44.999996	35
>>END_MODULE