SRR3551776_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397803
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGC	5714	1.4363893686070743	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCG	5402	1.3579585875420748	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTC	5159	1.2968730753664501	RNA PCR Primer, Index 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	2326	0.584711528067913	RNA PCR Primer, Index 40 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1555	0.3908970017822892	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGGATCT	1285	0.32302421047603963	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT	1271	0.3195048805564563	TruSeq Adapter, Index 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	1054	0.26495526680291503	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTA	978	0.24585033295374845	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	947	0.23805753098895688	RNA PCR Primer, Index 1 (96% over 26bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTC	917	0.23051610973270686	RNA PCR Primer, Index 40 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	809	0.20336699321020704	TruSeq Adapter, Index 1 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	540	0.13574558261249917	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGG	25	3.8861916E-5	45.0	2
CGTATGG	20	7.0277194E-4	45.0	2
GTATTAG	20	7.0277194E-4	45.0	1
ACCGCTC	20	7.0277194E-4	45.0	39
ACGGCTA	20	7.0277194E-4	45.0	30
CGCAGTT	25	3.8861916E-5	45.0	34
AGCGACT	45	3.8380676E-10	45.0	19
TTACCGG	25	3.8861916E-5	45.0	2
TCGGTAA	20	7.0277194E-4	45.0	43
TGCAACG	20	7.0277194E-4	45.0	1
TACCGAT	25	3.8861916E-5	45.0	14
CTCGAAT	80	0.0	42.1875	43
TACGGCT	675	0.0	41.666668	7
ACGGCTG	680	0.0	41.3603	8
CCGATGA	480	0.0	40.781246	18
TGATACC	615	0.0	40.609756	4
CCACCGA	650	0.0	40.153847	15
ACGGCCT	45	1.9228537E-8	40.0	8
GCGGGCA	90	0.0	40.0	5
GCGATGT	45	1.9228537E-8	40.0	9