##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397803
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205483618776128	31.0	31.0	33.0	28.0	34.0
2	31.110899113380242	31.0	31.0	34.0	28.0	34.0
3	31.001933117648687	31.0	31.0	34.0	27.0	34.0
4	34.8947820906328	35.0	35.0	37.0	32.0	37.0
5	34.56478206549473	35.0	35.0	37.0	32.0	37.0
6	34.77048941310146	36.0	35.0	37.0	32.0	37.0
7	35.33591249940297	37.0	35.0	37.0	33.0	37.0
8	34.76970007767664	37.0	35.0	37.0	32.0	37.0
9	36.64064876333261	39.0	37.0	39.0	32.0	39.0
10	36.258439478837516	37.0	35.0	39.0	32.0	39.0
11	36.58207454443531	38.0	35.0	39.0	32.0	39.0
12	36.686983255531004	39.0	35.0	39.0	33.0	39.0
13	36.71990658692871	39.0	35.0	39.0	33.0	39.0
14	37.66542735977356	39.0	36.0	41.0	33.0	41.0
15	37.71631938421782	39.0	36.0	41.0	33.0	41.0
16	37.85778890556381	39.0	37.0	41.0	33.0	41.0
17	37.63097060605375	39.0	36.0	41.0	33.0	41.0
18	37.635666397689306	39.0	36.0	41.0	33.0	41.0
19	37.607267919045356	39.0	36.0	41.0	33.0	41.0
20	37.52439524086043	39.0	36.0	41.0	32.0	41.0
21	37.43669605307149	39.0	36.0	41.0	32.0	41.0
22	37.57684833950473	39.0	36.0	41.0	33.0	41.0
23	37.6408222160215	39.0	36.0	41.0	33.0	41.0
24	37.53806029617675	39.0	35.0	41.0	33.0	41.0
25	37.201755642868456	39.0	35.0	41.0	32.0	41.0
26	37.34007033632225	39.0	35.0	41.0	32.0	41.0
27	37.38590960852482	39.0	35.0	41.0	33.0	41.0
28	37.266732528412305	39.0	35.0	41.0	32.0	41.0
29	37.35824264774273	39.0	35.0	41.0	32.0	41.0
30	36.99495981679374	39.0	35.0	41.0	31.0	41.0
31	36.97604090466889	39.0	35.0	41.0	31.0	41.0
32	36.98515094154644	39.0	35.0	41.0	31.0	41.0
33	36.96593540018552	39.0	35.0	41.0	31.0	41.0
34	36.733730012091414	39.0	35.0	41.0	31.0	41.0
35	36.784358086791705	39.0	35.0	41.0	31.0	41.0
36	36.68021105924289	39.0	35.0	41.0	31.0	41.0
37	36.771007257361056	39.0	35.0	41.0	31.0	41.0
38	36.48895810237731	39.0	35.0	41.0	30.0	41.0
39	36.48988821099891	39.0	35.0	41.0	30.0	41.0
40	36.43371719167528	39.0	35.0	41.0	30.0	41.0
41	36.167454745187946	39.0	35.0	40.0	29.0	41.0
42	36.39158327111661	39.0	35.0	41.0	30.0	41.0
43	36.42189475695256	39.0	35.0	41.0	31.0	41.0
44	36.51512180652232	39.0	35.0	41.0	31.0	41.0
45	36.52925191614945	39.0	35.0	41.0	31.0	41.0
46	36.38790054373647	39.0	35.0	41.0	31.0	41.0
47	36.22643368702599	39.0	35.0	40.0	30.0	41.0
48	36.21884953104929	39.0	35.0	40.0	30.0	41.0
49	36.318858832135504	39.0	35.0	40.0	31.0	41.0
50	36.20887725834144	39.0	35.0	40.0	30.0	41.0
51	35.304834302405965	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	29.0
10	27.0
11	18.0
12	27.0
13	20.0
14	32.0
15	26.0
16	44.0
17	74.0
18	115.0
19	222.0
20	566.0
21	1015.0
22	1581.0
23	1959.0
24	2489.0
25	3064.0
26	3611.0
27	4048.0
28	4633.0
29	5213.0
30	6674.0
31	8675.0
32	11537.0
33	16610.0
34	28330.0
35	30354.0
36	33872.0
37	51590.0
38	89093.0
39	92234.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.007689735874294	18.831180257564675	21.394760723272572	12.766369283288462
2	31.269749096914808	30.18629824310023	22.126781346545904	16.417171313439063
3	31.122691382417933	28.23533256410837	25.203429838387343	15.438546215086363
4	28.655138347372944	23.224309520038812	30.436170667390645	17.6843814651976
5	29.49826924382169	27.38415748498629	23.02144528824569	20.09612798294633
6	25.133294620704216	36.11712329972373	24.56743664577693	14.182145433795121
7	71.43435318486789	6.491152655962876	16.731899960533227	5.342594198636008
8	70.10253819101415	11.676131150343261	11.9448571277743	6.276473530868294
9	63.58524194136294	8.818686636350154	13.51045618057179	14.085615241715121
10	41.12487839458224	22.162226026450277	21.517937270457992	15.194958308509488
11	31.995987963891675	23.294444737721935	25.970643760856504	18.738923537529885
12	26.972898645812123	21.448807575609035	29.121449561717732	22.45684421686111
13	26.116193191102127	22.18208510242507	32.312476275945635	19.389245430527172
14	20.719803520838205	28.029954525229822	28.19988788420399	23.05035406972798
15	18.529272026606133	25.57974675907422	35.108081135637484	20.78290007868216
16	21.36987403312695	25.82383742706817	28.855740152789195	23.950548387015683
17	21.345992865815493	27.729554578522535	29.58524696897711	21.339205586684866
18	20.941772686480494	26.246408398126707	29.795401241317936	23.01641767407486
19	21.546846051940282	27.090544817409622	28.245639173158573	23.116969957491524
20	23.9259130775786	27.389687860574202	31.082973230468347	17.601425831378847
21	22.95709182685902	29.073938607803363	29.401236290324604	18.56773327501301
22	21.196169963524657	24.014399086985268	31.399461542522307	23.38996940696777
23	22.16373431070153	26.16998866273004	30.956277353363348	20.70999967320508
24	23.550098918308812	24.88493048066505	28.72929565639274	22.8356749446334
25	20.54534530911029	29.87760273301106	27.439461240865455	22.137590717013193
26	19.640374758360295	25.67250624052609	30.731542999927097	23.955576001186518
27	21.974193256461113	27.30849189171525	28.31552301013316	22.401791841690486
28	18.036817218573013	28.23759499048524	30.993984459644597	22.731603331297148
29	22.061673743033612	24.601624422138595	30.676993386173557	22.659708448654232
30	21.855541562029444	27.64408513761837	28.23583532552545	22.264537974826737
31	22.14915422960611	28.09682179370191	26.67124179556212	23.082782181129854
32	26.088541313162544	27.03876039145004	26.91156175292796	19.96113654245946
33	24.788903050002137	24.983471718413387	25.56416115514463	24.663464076439844
34	21.781635633718196	27.137804390615454	29.50229133515836	21.57826864050799
35	21.61723265033195	25.308004213140673	29.15714562233065	23.917617514196728
36	20.519453096130498	29.186808545938568	27.45554960621212	22.838188751718818
37	20.973698036465287	27.693607137201077	28.790883929985444	22.541810896348192
38	22.20043589414861	27.748659512371702	26.021925425399004	24.02897916808068
39	24.62198625953047	26.441731208663583	27.000801904460246	21.935480627345697
40	23.88091593074964	23.63607112063006	30.5505488897771	21.932464058843195
41	19.16903592984467	27.67274253839212	27.038509010741496	26.11971252102171
42	19.973956958595082	25.014894306981095	31.442196262974388	23.568952471449435
43	22.442766897182775	23.96965332086485	27.210956176801083	26.376623605151295
44	21.772585928210695	24.30147585613985	28.628743372976075	25.29719484267338
45	21.900790089566947	23.799468581182143	27.64584480257816	26.65389652667275
46	24.436718677335264	26.38969540199546	26.95882132613379	22.214764594535485
47	19.160488985754256	25.00081698730276	33.17848281687167	22.660211210071317
48	20.584309318934245	25.35375550209526	28.866298142547947	25.195637036422553
49	21.749207522316322	22.95357249693944	32.50101180735188	22.79620817339236
50	21.160725283620284	23.315560717239435	29.796909525569188	25.72680447357109
51	19.90080517240946	22.828636284794232	29.102344627868572	28.16821391492774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	354.5
2	590.0
3	1810.0
4	3030.0
5	2084.0
6	1138.0
7	1099.0
8	1060.0
9	1037.5
10	1015.0
11	1015.0
12	1015.0
13	1006.0
14	997.0
15	947.0
16	897.0
17	969.5
18	1042.0
19	1147.0
20	1252.0
21	1430.5
22	1609.0
23	1728.0
24	1847.0
25	2337.5
26	3436.5
27	4045.0
28	4603.0
29	5161.0
30	5836.0
31	6511.0
32	7837.5
33	9164.0
34	10048.5
35	10933.0
36	11419.0
37	11905.0
38	13108.5
39	14312.0
40	15548.5
41	16785.0
42	18364.5
43	19944.0
44	22200.0
45	24456.0
46	37665.5
47	50875.0
48	44257.0
49	37639.0
50	37324.5
51	37010.0
52	31892.5
53	26775.0
54	24496.0
55	22217.0
56	20401.5
57	18586.0
58	17547.5
59	16509.0
60	15335.5
61	14162.0
62	12580.0
63	10998.0
64	9381.5
65	7765.0
66	6185.5
67	4606.0
68	3868.0
69	3130.0
70	2790.0
71	2450.0
72	1959.5
73	1469.0
74	1116.0
75	679.5
76	596.0
77	455.0
78	314.0
79	231.0
80	148.0
81	117.5
82	87.0
83	62.5
84	38.0
85	23.0
86	8.0
87	5.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	397803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.39881088977179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59402746129935	25.315470332579082
2	9.136839451577039	6.2859282485000945
3	3.1766930975129704	3.2782339534857616
4	1.8651649199200653	2.566378214342666
5	1.337817487514259	2.3009665379016315
6	1.1334681070586465	2.3393973038578797
7	0.9646560854786148	2.3228115580632482
8	0.8949185897833143	2.462730826535798
9	0.8150788904806114	2.5233970152498806
>10	6.975765849070763	40.12620243670442
>50	0.061274930106713435	1.4691005639215156
>100	0.034697851988138934	1.9097037581140528
>500	0.003691260849802014	1.0643043151936165
>1k	0.003691260849802014	1.9023392090468576
>5k	0.0022147565098812085	4.133035726503486
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGC	5714	1.4363893686070743	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCG	5402	1.3579585875420748	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTC	5159	1.2968730753664501	RNA PCR Primer, Index 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	2326	0.584711528067913	RNA PCR Primer, Index 40 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1555	0.3908970017822892	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGGATCT	1285	0.32302421047603963	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT	1271	0.3195048805564563	TruSeq Adapter, Index 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	1054	0.26495526680291503	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTA	978	0.24585033295374845	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	947	0.23805753098895688	RNA PCR Primer, Index 1 (96% over 26bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTC	917	0.23051610973270686	RNA PCR Primer, Index 40 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	809	0.20336699321020704	TruSeq Adapter, Index 1 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	540	0.13574558261249917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21141117588354036	0.0
2	0.0	0.0	0.0	1.224224050597909	0.0
3	0.0	0.0	0.0	1.5542869209131154	0.0
4	0.0	0.0	0.0	2.2599125697895692	0.0
5	0.0	0.0	0.0	4.74657053868372	0.0
6	0.0	0.0	0.0	5.493422623761007	0.0
7	0.0	0.0	0.0	6.467774250068501	0.0
8	0.0	0.0	0.0	7.557509621596619	0.0
9	0.0	0.0	0.0	7.8910918218314094	0.0
10	0.0	0.0	0.0	10.012996382631604	0.0
11	0.0	0.0	0.0	11.296043519028263	0.0
12	0.0	0.0	0.0	13.755803752108456	0.0
13	0.0	0.0	0.0	14.268369016824911	0.0
14	0.0	0.0	0.0	14.510448639150534	0.0
15	0.0	0.0	0.0	15.117281669570113	0.0
16	0.0	0.0	0.0	15.823912841280734	0.0
17	0.0	0.0	0.0	16.582077058242398	0.0
18	0.0	0.0	0.0	17.289462372078642	0.0
19	0.0	0.0	0.0	18.18437769448697	0.0
20	2.513807085416651E-4	0.0	0.0	18.761296420590092	0.0
21	2.513807085416651E-4	0.0	0.0	19.397792374617588	0.0
22	2.513807085416651E-4	0.0	0.0	20.07928547547404	0.0
23	2.513807085416651E-4	0.0	0.0	20.682347795265496	0.0
24	2.513807085416651E-4	0.0	0.0	21.180081598177992	0.0
25	2.513807085416651E-4	0.0	0.0	21.622763025919866	0.0
26	2.513807085416651E-4	0.0	0.0	22.040809144224653	0.0
27	2.513807085416651E-4	0.0	0.0	22.531504287297984	0.0
28	2.513807085416651E-4	0.0	0.0	22.98700613117548	0.0
29	2.513807085416651E-4	0.0	0.0	23.438737264424855	0.0
30	2.513807085416651E-4	0.0	0.0	23.919377179156516	0.0
31	2.513807085416651E-4	0.0	0.0	24.39222429192339	0.0
32	2.513807085416651E-4	0.0	0.0	24.806751080308594	0.0
33	2.513807085416651E-4	0.0	0.0	25.24390213246255	0.0
34	2.513807085416651E-4	0.0	0.0	25.68457251453609	0.0
35	2.513807085416651E-4	0.0	0.0	26.112673861182547	0.0
36	2.513807085416651E-4	0.0	0.0	26.53122274090442	0.0
37	2.513807085416651E-4	0.0	0.0	26.950777143460456	0.0
38	2.513807085416651E-4	0.0	0.0	27.400245850332954	0.0
39	2.513807085416651E-4	0.0	0.0	27.912811115049408	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGG	25	3.8861916E-5	45.0	2
CGTATGG	20	7.0277194E-4	45.0	2
GTATTAG	20	7.0277194E-4	45.0	1
ACCGCTC	20	7.0277194E-4	45.0	39
ACGGCTA	20	7.0277194E-4	45.0	30
CGCAGTT	25	3.8861916E-5	45.0	34
AGCGACT	45	3.8380676E-10	45.0	19
TTACCGG	25	3.8861916E-5	45.0	2
TCGGTAA	20	7.0277194E-4	45.0	43
TGCAACG	20	7.0277194E-4	45.0	1
TACCGAT	25	3.8861916E-5	45.0	14
CTCGAAT	80	0.0	42.1875	43
TACGGCT	675	0.0	41.666668	7
ACGGCTG	680	0.0	41.3603	8
CCGATGA	480	0.0	40.781246	18
TGATACC	615	0.0	40.609756	4
CCACCGA	650	0.0	40.153847	15
ACGGCCT	45	1.9228537E-8	40.0	8
GCGGGCA	90	0.0	40.0	5
GCGATGT	45	1.9228537E-8	40.0	9
>>END_MODULE