FastQCFastQC Report
Sat 18 Jun 2016
SRR3551773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603470
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG88111.4600560094122326No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC83731.3874757651581686No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC81931.357648267519512No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.6674731138250452No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC32080.5315922912489436Illumina PCR Primer Index 3 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT20980.34765605581056225No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT17620.29197806021840356No Hit
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT17430.2888296021343232TruSeq Adapter, Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC14620.24226556415397615RNA PCR Primer, Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA13790.22851177357615127No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC11970.1983528592970653No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG11050.18310769383730757TruSeq Adapter, Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT11010.18244486055644854TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC7680.12726398992493412No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC6630.10986461630238455No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA6120.10141349197143189No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC253.8885955E-545.030
TTTACGG351.2106466E-745.02
GCGAAGT207.030621E-445.01
TTCGCAT253.8885955E-545.029
CAATACG207.030621E-445.01
CGATAGA207.030621E-445.010
TATAGCG302.1637807E-644.9999961
ATACGGA302.1637807E-644.99999628
CTCGAAT1800.043.7543
CGTTTTT28350.043.4920651
TACGGCT10350.043.478267
ACGGCTG10350.043.478268
CCTCGAA1900.042.6315842
CGGCGAT850.042.35294331
CCGATGA8900.042.219118
CGTTATT800.042.18751
CGAATGC2100.041.78571345
TCGAATG2000.041.62544
CAACGCA650.041.5384617
CGATGAA9050.041.5193419
Produced by FastQC (version 0.11.5)