##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	603470
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27963941869521	31.0	31.0	33.0	28.0	34.0
2	31.200603178285583	31.0	31.0	34.0	28.0	34.0
3	31.05287918206373	31.0	31.0	34.0	28.0	34.0
4	34.91726017863357	35.0	35.0	37.0	32.0	37.0
5	34.59647206986263	35.0	35.0	37.0	32.0	37.0
6	34.836091272142774	36.0	35.0	37.0	32.0	37.0
7	35.386363862329524	37.0	35.0	37.0	33.0	37.0
8	34.93343165360333	37.0	35.0	37.0	32.0	37.0
9	36.87991946575637	39.0	37.0	39.0	32.0	39.0
10	36.39254478267354	37.0	35.0	39.0	32.0	39.0
11	36.69006910036953	38.0	35.0	39.0	32.0	39.0
12	36.84957661524185	39.0	37.0	39.0	33.0	39.0
13	36.90961605382206	39.0	37.0	39.0	33.0	39.0
14	37.94650106881866	40.0	37.0	41.0	33.0	41.0
15	37.97672460934263	40.0	37.0	41.0	33.0	41.0
16	38.11929839097221	40.0	37.0	41.0	34.0	41.0
17	37.861050259333524	40.0	37.0	41.0	33.0	41.0
18	37.82719936368005	39.0	37.0	41.0	33.0	41.0
19	37.74416126733723	39.0	37.0	41.0	33.0	41.0
20	37.62621174209157	39.0	36.0	41.0	33.0	41.0
21	37.575770129418196	39.0	36.0	41.0	33.0	41.0
22	37.69190349147431	39.0	36.0	41.0	33.0	41.0
23	37.72526554758314	39.0	36.0	41.0	33.0	41.0
24	37.63488491557161	39.0	36.0	41.0	33.0	41.0
25	37.33667787959634	39.0	35.0	41.0	33.0	41.0
26	37.464208659916814	39.0	36.0	41.0	33.0	41.0
27	37.48844350174822	39.0	36.0	41.0	33.0	41.0
28	37.37281886423517	39.0	35.0	41.0	33.0	41.0
29	37.463226009577944	39.0	36.0	41.0	33.0	41.0
30	37.09904220590916	39.0	35.0	41.0	32.0	41.0
31	37.08809054302616	39.0	35.0	41.0	32.0	41.0
32	36.962047823421216	39.0	35.0	41.0	31.0	41.0
33	36.853490645765326	39.0	35.0	41.0	31.0	41.0
34	36.658543092448674	40.0	35.0	41.0	30.0	41.0
35	36.57156776641755	40.0	35.0	41.0	30.0	41.0
36	36.4501532801962	39.0	35.0	41.0	30.0	41.0
37	36.459048502825325	39.0	35.0	41.0	30.0	41.0
38	36.33037930634497	39.0	35.0	41.0	30.0	41.0
39	36.31438348219464	39.0	35.0	41.0	30.0	41.0
40	36.31552521252092	39.0	35.0	41.0	30.0	41.0
41	36.08033042239051	39.0	35.0	41.0	28.0	41.0
42	36.23664142376589	39.0	35.0	41.0	30.0	41.0
43	36.2179810098265	39.0	35.0	41.0	30.0	41.0
44	36.29952938837059	39.0	35.0	41.0	30.0	41.0
45	36.352542794173694	39.0	35.0	41.0	30.0	41.0
46	36.192132168956206	39.0	35.0	41.0	30.0	41.0
47	36.03273733574163	39.0	35.0	41.0	29.0	41.0
48	36.03573002800471	39.0	35.0	41.0	29.0	41.0
49	36.09540325119724	39.0	35.0	41.0	29.0	41.0
50	36.019686148441515	39.0	35.0	41.0	29.0	41.0
51	35.18485260244917	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	20.0
10	33.0
11	26.0
12	25.0
13	19.0
14	26.0
15	28.0
16	69.0
17	98.0
18	184.0
19	403.0
20	954.0
21	1688.0
22	2621.0
23	3563.0
24	4652.0
25	5929.0
26	7029.0
27	7612.0
28	7426.0
29	7771.0
30	9167.0
31	12021.0
32	15684.0
33	22649.0
34	37312.0
35	41976.0
36	49210.0
37	77079.0
38	137503.0
39	150664.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04709430460503	18.866057964770413	21.01347208643346	16.073375644191096
2	31.81914593931761	29.93172817207152	22.517109384062174	15.732016504548692
3	29.692445357681407	28.862246673405473	26.844416458150366	14.600891510762755
4	27.02884981854939	22.98274976386564	33.19535353870118	16.79304687888379
5	29.23525610220889	27.410807496644406	25.274992957396393	18.078943443750312
6	24.46070227186107	36.40363232637911	25.519909854673806	13.61575554708602
7	71.52053954629062	5.858451952872554	17.896995708154506	4.724012792682321
8	71.48640363232637	11.099971829585563	12.326710524135418	5.08691401395264
9	65.82100187250401	7.74736109500058	13.72827149651184	12.703365535983563
10	38.89141133776327	25.90253036604968	21.392612723084824	13.813445573102227
11	29.13980810976519	23.836313321291865	28.977248247634513	18.04663032130843
12	25.188824630884714	21.42476013720649	31.18597444777702	22.20044078413177
13	24.726332709165327	22.130677581321358	34.70097933617247	18.442010373340846
14	19.77844797587287	28.663562397467977	30.433327257361594	21.12466236929756
15	18.441181831739772	25.48577394070956	36.211244966609776	19.86179926094089
16	21.15912969990223	26.843090791588647	29.448191293684857	22.54958821482427
17	20.988118713440603	28.28508459409747	31.05357349992543	19.673223192536497
18	20.61030374335095	26.911528327837342	30.69647206986263	21.781695858949078
19	21.079589706199148	28.184002518766466	28.609210068437534	22.12719770659685
20	23.4077916052165	28.022271198236865	32.196132367806186	16.37380482874045
21	23.06129550764744	29.866439093906905	29.76734551841848	17.304919880027175
22	21.146867284206337	23.924138731005684	32.70949674383151	22.219497240956468
23	22.14956832982584	27.298622963859014	31.550698460569706	19.00111024574544
24	22.047657712893766	25.362155533829355	30.268447478747905	22.321739274528973
25	19.81788655608398	31.4945233400169	27.930965913798534	20.756624190100585
26	19.66046365187996	26.28631083566706	32.468391137919035	21.584834374533944
27	22.377914395081778	28.367607337564422	29.001938787346514	20.252539480007293
28	18.557840489170964	28.73299418363796	31.966957760949178	20.742207566241902
29	21.102457454388784	25.309791704641487	32.640893499262596	20.946857341707126
30	20.24856248032214	29.868593302069694	30.322302682817703	19.560541534790463
31	22.76567186438431	26.368667870813795	27.852751586657163	23.01290867814473
32	22.961207682237724	27.55580227683232	29.72906689644887	19.753923144481085
33	21.711601239498236	25.789185875022785	26.933236117785476	25.565976767693506
34	21.169072199115117	27.173181765456444	29.826503388735148	21.83124264669329
35	20.01110245745439	26.80895487762441	28.355676338508957	24.82426632641225
36	20.22287769068885	28.82313950983479	27.97189586889158	22.982086930584785
37	19.72011864715727	28.597279069382076	30.56523108025254	21.117371203208112
38	21.452764843322782	25.84652095381709	26.831822625814045	25.86889157704608
39	21.647140702934696	26.461298821813845	29.66742340132898	22.22413707392248
40	20.463320463320464	24.947387608331812	29.150082025618506	25.439209902729214
41	19.002767328947588	28.597776194342718	27.069945481962648	25.329510994747046
42	19.772813892985567	25.79432283294944	29.983097751338093	24.449765522726896
43	21.414154804712744	25.24566258471838	28.120867648764648	25.219314961804233
44	21.13891328483603	25.816362039538003	29.28761993139676	23.757104744229206
45	20.88189968018294	23.837970404494012	28.6469915654465	26.633138349876546
46	23.089797338724377	27.03315823487497	27.702454140222383	22.17459028617827
47	18.795797636999353	26.07784976883689	33.090625880325454	22.0357267138383
48	20.801862561519215	25.95207715379389	28.665550897310553	24.58050938737634
49	20.00629691616816	23.50357101430063	32.98523538866887	23.50489668086235
50	20.359255638225594	24.020580973370674	30.18592473528096	25.434238653122776
51	19.646709861302135	23.43016222844549	28.767461514242633	28.155666396009742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	422.0
2	722.0
3	4139.0
4	7556.0
5	5261.0
6	2966.0
7	2870.5
8	2775.0
9	2700.5
10	2626.0
11	2641.5
12	2657.0
13	2508.5
14	2360.0
15	2267.0
16	2174.0
17	2193.0
18	2212.0
19	2328.0
20	2444.0
21	2853.0
22	3262.0
23	3298.0
24	3334.0
25	4212.5
26	5473.0
27	5855.0
28	7468.5
29	9082.0
30	10430.0
31	11778.0
32	12706.5
33	13635.0
34	15511.0
35	17387.0
36	19404.0
37	21421.0
38	22726.5
39	24032.0
40	26189.5
41	28347.0
42	29945.5
43	31544.0
44	35187.5
45	38831.0
46	58162.5
47	77494.0
48	66709.0
49	55924.0
50	54265.5
51	52607.0
52	45407.5
53	38208.0
54	34491.0
55	30774.0
56	27897.0
57	25020.0
58	22884.0
59	20748.0
60	19279.5
61	17811.0
62	16458.0
63	15105.0
64	12471.5
65	9838.0
66	7979.5
67	6121.0
68	4821.0
69	3521.0
70	3195.0
71	2869.0
72	2518.0
73	2167.0
74	1808.5
75	1071.5
76	693.0
77	603.0
78	513.0
79	365.5
80	218.0
81	155.0
82	92.0
83	76.0
84	60.0
85	39.0
86	18.0
87	11.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	603470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.614828856585504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41644551099597	26.503289709895423
2	8.54809100532072	6.088775964100307
3	3.3127673397465487	3.5395092554027814
4	1.9252822472818891	2.742743909502672
5	1.4690982583214633	2.6160841521813376
6	1.1569132898748364	2.472196129248095
7	0.9828764088827445	2.450348256267401
8	0.8506192389279039	2.423572689322905
9	0.8051164899911768	2.5806677400585536
>10	6.444396537945201	37.50743866940573
>50	0.054070597669744744	1.3344368252021634
>100	0.025389671948745706	1.680898086481315
>500	0.0028210746609717455	0.6150566518874148
>1k	0.004701791101619576	3.195514861956857
>5k	0.0014105373304858727	4.249467099087101
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	8811	1.4600560094122326	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	8373	1.3874757651581686	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	8193	1.357648267519512	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4028	0.6674731138250452	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	3208	0.5315922912489436	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT	2098	0.34765605581056225	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	1762	0.29197806021840356	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	1743	0.2888296021343232	TruSeq Adapter, Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1462	0.24226556415397615	RNA PCR Primer, Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA	1379	0.22851177357615127	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC	1197	0.1983528592970653	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	1105	0.18310769383730757	TruSeq Adapter, Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	1101	0.18244486055644854	TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	768	0.12726398992493412	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	663	0.10986461630238455	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	612	0.10141349197143189	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18758181848310604	0.0
2	0.0	0.0	0.0	1.0958291215801945	0.0
3	0.0	0.0	0.0	1.4648615506984606	0.0
4	0.0	0.0	0.0	2.124049248512768	0.0
5	0.0	0.0	0.0	4.4848956866124245	0.0
6	0.0	0.0	0.0	5.362486950469783	0.0
7	0.0	0.0	0.0	6.351102788871029	0.0
8	0.0	0.0	0.0	7.42207566241901	0.0
9	0.0	0.0	0.0	7.800884882429947	0.0
10	0.0	0.0	0.0	10.033970205644025	0.0
11	0.0	0.0	0.0	11.16492949110975	0.0
12	0.0	0.0	0.0	13.480537557790777	0.0
13	0.0	0.0	0.0	13.915687606674732	0.0
14	0.0	0.0	0.0	14.150496296419043	0.0
15	0.0	0.0	0.0	14.667506255489089	0.0
16	0.0	0.0	0.0	15.257262167133412	0.0
17	0.0	0.0	0.0	15.914792781745572	0.0
18	0.0	0.0	0.0	16.569672063234297	0.0
19	0.0	0.0	0.0	17.419590037615787	0.0
20	0.0	0.0	0.0	17.91969774802393	0.0
21	0.0	0.0	0.0	18.459409746963395	0.0
22	0.0	0.0	0.0	19.094901154986992	0.0
23	0.0	0.0	0.0	19.67024044277263	0.0
24	0.0	0.0	0.0	20.119144282234412	0.0
25	0.0	0.0	0.0	20.53391220773195	0.0
26	0.0	0.0	0.0	20.926972343281356	0.0
27	0.0	0.0	0.0	21.334117686049016	0.0
28	0.0	0.0	0.0	21.726514988317565	0.0
29	0.0	0.0	0.0	22.12520920675427	0.0
30	0.0	0.0	0.0	22.58206704558636	0.0
31	0.0	0.0	0.0	23.010257345021294	0.0
32	0.0	0.0	0.0	23.43463635309129	0.0
33	0.0	0.0	0.0	23.846587237145176	0.0
34	0.0	0.0	0.0	24.241801580857373	0.0
35	0.0	0.0	0.0	24.63900442441215	0.0
36	0.0	0.0	0.0	25.04233847581487	0.0
37	0.0	0.0	0.0	25.45163802674532	0.0
38	0.0	0.0	0.0	25.889936533713357	0.0
39	0.0	0.0	0.0	26.3361890400517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATC	25	3.8885955E-5	45.0	30
TTTACGG	35	1.2106466E-7	45.0	2
GCGAAGT	20	7.030621E-4	45.0	1
TTCGCAT	25	3.8885955E-5	45.0	29
CAATACG	20	7.030621E-4	45.0	1
CGATAGA	20	7.030621E-4	45.0	10
TATAGCG	30	2.1637807E-6	44.999996	1
ATACGGA	30	2.1637807E-6	44.999996	28
CTCGAAT	180	0.0	43.75	43
CGTTTTT	2835	0.0	43.492065	1
TACGGCT	1035	0.0	43.47826	7
ACGGCTG	1035	0.0	43.47826	8
CCTCGAA	190	0.0	42.63158	42
CGGCGAT	85	0.0	42.352943	31
CCGATGA	890	0.0	42.2191	18
CGTTATT	80	0.0	42.1875	1
CGAATGC	210	0.0	41.785713	45
TCGAATG	200	0.0	41.625	44
CAACGCA	65	0.0	41.53846	17
CGATGAA	905	0.0	41.51934	19
>>END_MODULE