##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551771_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	555873
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23927407879138	31.0	31.0	33.0	28.0	34.0
2	31.153004373301094	31.0	31.0	34.0	28.0	34.0
3	30.99894220442439	31.0	31.0	34.0	27.0	34.0
4	34.813045425843676	35.0	35.0	37.0	32.0	37.0
5	34.58115612738881	35.0	35.0	37.0	32.0	37.0
6	34.80242429475798	36.0	35.0	37.0	32.0	37.0
7	35.40801765870982	37.0	35.0	37.0	33.0	37.0
8	34.992793317898155	37.0	35.0	37.0	32.0	37.0
9	36.92775687971893	39.0	37.0	39.0	32.0	39.0
10	36.407895328609236	37.0	35.0	39.0	32.0	39.0
11	36.681889568300676	38.0	35.0	39.0	32.0	39.0
12	36.838258019367736	39.0	37.0	39.0	33.0	39.0
13	36.91139342979422	39.0	37.0	39.0	33.0	39.0
14	37.89682355502066	40.0	37.0	41.0	33.0	41.0
15	37.97621399132536	40.0	37.0	41.0	33.0	41.0
16	38.04910114360654	40.0	37.0	41.0	33.0	41.0
17	37.854155535527	39.0	37.0	41.0	33.0	41.0
18	37.85348092100174	39.0	37.0	41.0	33.0	41.0
19	37.778505162150346	39.0	37.0	41.0	33.0	41.0
20	37.75916981037035	39.0	36.0	41.0	33.0	41.0
21	37.63727865897426	39.0	36.0	41.0	33.0	41.0
22	37.816929406537106	39.0	36.0	41.0	33.0	41.0
23	37.85563249159431	40.0	36.0	41.0	33.0	41.0
24	37.74891746855847	40.0	36.0	41.0	33.0	41.0
25	37.35543550415293	39.0	36.0	41.0	32.0	41.0
26	37.525906097256026	39.0	36.0	41.0	33.0	41.0
27	37.60204759000707	39.0	36.0	41.0	33.0	41.0
28	37.471282109402686	39.0	36.0	41.0	33.0	41.0
29	37.55224124935012	40.0	36.0	41.0	33.0	41.0
30	37.16963047314764	39.0	35.0	41.0	32.0	41.0
31	37.184945122357085	39.0	35.0	41.0	32.0	41.0
32	37.18559635024547	39.0	36.0	41.0	32.0	41.0
33	37.09959649056529	39.0	36.0	41.0	31.0	41.0
34	37.032876214531015	40.0	36.0	41.0	31.0	41.0
35	37.03424523227428	40.0	36.0	41.0	31.0	41.0
36	36.85040647773862	39.0	35.0	41.0	31.0	41.0
37	36.87674342880478	40.0	35.0	41.0	31.0	41.0
38	36.565674173777104	39.0	35.0	41.0	30.0	41.0
39	36.61917560306041	39.0	35.0	41.0	31.0	41.0
40	36.52651774775893	39.0	35.0	41.0	30.0	41.0
41	36.350446954610135	39.0	35.0	41.0	30.0	41.0
42	36.4807339086446	39.0	35.0	41.0	30.0	41.0
43	36.46536349130107	39.0	35.0	41.0	31.0	41.0
44	36.53329627450874	39.0	35.0	41.0	31.0	41.0
45	36.530952213904975	39.0	35.0	41.0	31.0	41.0
46	36.398983220987525	39.0	35.0	41.0	30.0	41.0
47	36.247275906546996	39.0	35.0	41.0	30.0	41.0
48	36.25140094949746	39.0	35.0	41.0	30.0	41.0
49	36.25952510735366	39.0	35.0	41.0	30.0	41.0
50	36.17155897120385	39.0	35.0	40.0	30.0	41.0
51	35.25158444464833	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	24.0
10	27.0
11	20.0
12	21.0
13	19.0
14	25.0
15	36.0
16	53.0
17	97.0
18	149.0
19	359.0
20	682.0
21	1263.0
22	1832.0
23	2388.0
24	3097.0
25	4165.0
26	5340.0
27	6103.0
28	6399.0
29	7304.0
30	8873.0
31	11399.0
32	15170.0
33	21423.0
34	34585.0
35	40009.0
36	47717.0
37	73372.0
38	127873.0
39	136013.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37689724091654	21.681031458624542	23.948275955119243	12.993795345339674
2	31.65723105817336	28.27570326315543	24.259318225565913	15.807747453105295
3	30.483221887013762	27.164298319939988	27.419752353505206	14.932727439541047
4	27.55521495017747	23.734917867930264	31.041083125102315	17.66878405678995
5	28.175320621796708	29.43046343319427	24.55291046695918	17.841305478049843
6	25.26476371401381	35.619826830948796	25.09188249834045	14.023526956696944
7	74.19374569371061	6.124240608916065	14.889372212717653	4.792641484655668
8	74.69314033960994	9.265965427354809	10.712518866719556	5.328375366315687
9	68.1036855540744	8.150782642797905	12.64047723131003	11.105054571817663
10	38.727191282900954	28.052270932389234	19.5776733174664	13.642864467243419
11	27.705429117802087	24.586011552998617	28.898327495668973	18.810231833530324
12	24.587090936239033	21.845637402788046	31.564404099497544	22.002867561475373
13	24.109463852354764	23.18605149017851	33.92393586304785	18.78054879441887
14	19.807042256054892	27.717482230653406	29.92769931261277	22.547776200678932
15	17.837527636708383	26.111180071707025	35.34440420743587	20.706888084148716
16	19.9806430605552	26.67767637571891	29.513216148292866	23.82846441543302
17	20.436862376837876	27.52157417251782	30.288393212118596	21.75317023852571
18	19.89267332646126	26.099846547682652	31.60362169056601	22.403858435290076
19	20.60272760144853	26.847859133291234	29.45582893934406	23.093584325916172
20	22.362302180534044	27.340237788127865	32.43168853317214	17.865771498165948
21	22.11746208216625	28.67813331462402	30.533952899313334	18.670451703896394
22	21.031602542307326	23.383578623174717	31.316865543028715	24.267953291489246
23	21.329512316662257	26.454603839366186	31.061411509463493	21.15447233450806
24	21.51030900943201	25.156105801145223	30.619583969719706	22.71400121970306
25	19.400114774417897	30.37060623559698	28.079075616192906	22.150203373792216
26	20.22836151423077	26.99555474002155	30.240900349540272	22.535183396207405
27	23.209258229847464	27.673227517796334	28.42825609446762	20.68925815788858
28	18.622778944111335	28.239543924601485	31.576457212348863	21.561219918938317
29	23.839797939457394	23.95115431042702	29.497744988513563	22.71130276160202
30	22.670825890086405	28.665720407359235	28.676334342556665	19.987119359997696
31	22.781642569435824	27.628073318905578	26.5015570103243	23.088727101334296
32	25.008769988828384	28.00747652791195	25.864541001271874	21.11921248198779
33	22.05233929332779	28.481865462074968	26.291077278443097	23.17471796615414
34	22.07626562182369	26.62982371872712	29.00482664205673	22.28908401739246
35	23.926508393104182	25.07461236649379	26.857033890834774	24.141845349567255
36	22.517013778327062	30.72176558314579	26.43877288517341	20.322447753353735
37	23.117690551618804	26.780757475178685	30.20096316964486	19.900588803557646
38	20.788021724386684	29.421468572857467	26.21048332982534	23.580026372930508
39	23.19990357509719	26.788133260654863	28.879798083375157	21.132165080872788
40	21.820451793844995	26.173964196857913	29.23923270243383	22.766351306863257
41	19.536117062710368	27.819124152459285	28.702419437533393	23.942339347296954
42	21.31350146526275	25.92822461245644	29.22250226220737	23.535771660073436
43	23.5445866232035	27.140012197030615	26.84947820815186	22.46592297161402
44	22.135991494460068	25.720083544262806	28.46171697492053	23.682207986356595
45	21.040597402644128	25.364426766545595	27.891083035153713	25.703892795656564
46	24.685854502737136	26.977385122141207	27.034412536676545	21.30234783844511
47	19.444909178895177	26.34810469297843	32.74596895334006	21.461017174786328
48	20.925463190333044	25.916531294018597	29.045483410779084	24.112522104869278
49	21.036459766889198	24.140945863533577	31.83982672301047	22.982767646566753
50	21.15177387640702	24.059632326088874	30.423855808790854	24.36473798871325
51	20.797196481930225	23.43376994385408	27.79753648765096	27.97149708656474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	353.0
2	527.0
3	3068.5
4	5610.0
5	3835.0
6	2060.0
7	1974.5
8	1889.0
9	1864.0
10	1839.0
11	1790.5
12	1742.0
13	1747.0
14	1752.0
15	1687.5
16	1623.0
17	1521.5
18	1420.0
19	1562.0
20	1704.0
21	2574.5
22	3445.0
23	3123.0
24	2801.0
25	3562.0
26	5061.5
27	5800.0
28	7008.5
29	8217.0
30	10082.0
31	11947.0
32	12631.0
33	13315.0
34	15046.5
35	16778.0
36	18063.0
37	19348.0
38	20677.5
39	22007.0
40	24392.0
41	26777.0
42	28474.0
43	30171.0
44	32780.5
45	35390.0
46	50734.5
47	66079.0
48	60293.5
49	54508.0
50	52573.5
51	50639.0
52	43438.5
53	36238.0
54	31533.5
55	26829.0
56	24510.5
57	22192.0
58	20613.5
59	19035.0
60	17886.5
61	16738.0
62	14830.0
63	12922.0
64	10794.0
65	8666.0
66	7577.5
67	6489.0
68	5433.0
69	4377.0
70	3944.5
71	3512.0
72	3029.0
73	2546.0
74	2215.0
75	1610.0
76	1336.0
77	996.0
78	656.0
79	483.5
80	311.0
81	220.5
82	130.0
83	94.0
84	58.0
85	45.5
86	33.0
87	27.5
88	22.0
89	15.0
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	555873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.288920372583174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99493919340973	23.152217603967312
2	9.845196093522052	6.160911132453569
3	3.385744944368484	3.1780891189866467
4	1.7284155881762742	2.1632103083671583
5	1.0972907279960438	1.7166521106921018
6	0.855091285299462	1.6052929882214801
7	0.632125805874893	1.3844973803832146
8	0.5571615788776552	1.3946387420932538
9	0.4957583792188599	1.3960570006277822
>10	7.2556824299123175	46.929168710055606
>50	0.10037550337857301	2.0617492105548494
>100	0.04351539163810966	2.430456848719584
>500	0.0017406156655243863	0.3944863110447906
>1k	0.0052218469965731595	2.750511780321961
>5k	0.0017406156655243863	3.282060753510708
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGC	6491	1.167712768923837	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCG	6004	1.0801028292433703	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTC	5584	1.0045460024142205	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3369	0.6060736894938232	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC	3156	0.5677555844590401	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGATCAT	1633	0.29377213860000395	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC	1353	0.24340092071390407	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCT	1252	0.2252313028335609	No Hit
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT	1180	0.2122787039485638	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT	1088	0.19572816092884526	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGATCATCGTA	1076	0.1935693944480124	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTC	1044	0.18781268383245814	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCC	882	0.15866933634121463	No Hit
TCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC	680	0.12233010058052829	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	611	0.10991719331573939	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21947459221800664	0.0
2	0.0	0.0	0.0	1.284825850509019	0.0
3	0.0	0.0	0.0	1.62681044051429	0.0
4	0.0	0.0	0.0	2.2755197680045622	0.0
5	0.0	0.0	0.0	4.421873341572625	0.0
6	0.0	0.0	0.0	5.124192036670246	0.0
7	0.0	0.0	0.0	6.018101256941783	0.0
8	0.0	0.0	0.0	7.189591867207078	0.0
9	0.0	0.0	0.0	7.579968805824352	0.0
10	0.0	0.0	0.0	9.533292676564612	0.0
11	0.0	0.0	0.0	10.874066558368549	0.0
12	0.0	0.0	0.0	13.120622876088603	0.0
13	0.0	0.0	0.0	13.629552073944948	0.0
14	0.0	0.0	0.0	13.84830707733601	0.0
15	0.0	0.0	0.0	14.377204865140058	0.0
16	0.0	0.0	0.0	15.06081425073713	0.0
17	0.0	0.0	0.0	15.871071269876392	0.0
18	0.0	0.0	0.0	16.66981486778455	0.0
19	0.0	0.0	0.0	17.55472922771928	0.0
20	0.0	0.0	0.0	18.13579720547679	0.0
21	0.0	0.0	0.0	18.760220410057695	0.0
22	0.0	0.0	0.0	19.467396329737188	0.0
23	0.0	0.0	0.0	20.115565965607253	0.0
24	0.0	0.0	0.0	20.64356426737762	0.0
25	0.0	0.0	0.0	21.143858399310634	0.0
26	0.0	0.0	0.0	21.574352415030052	0.0
27	0.0	0.0	0.0	22.049101143606542	0.0
28	0.0	0.0	0.0	22.484812178321306	0.0
29	0.0	0.0	0.0	22.931676839853708	0.0
30	0.0	0.0	0.0	23.451579767320954	0.0
31	0.0	0.0	0.0	23.913375897012447	0.0
32	0.0	0.0	0.0	24.38254781218012	0.0
33	0.0	0.0	0.0	24.815560388793845	0.0
34	0.0	0.0	0.0	25.253610087196176	0.0
35	0.0	0.0	0.0	25.714326833647252	0.0
36	0.0	0.0	0.0	26.18116008512736	0.0
37	0.0	0.0	0.0	26.643675803645795	0.0
38	0.0	0.0	0.0	27.082265193668338	0.0
39	0.0	0.0	0.0	27.532368004921988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	30	2.1634714E-6	45.000004	16
TTGCGTG	30	2.1634714E-6	45.000004	1
GCGAACA	25	3.8881975E-5	45.0	33
CGCATCC	20	7.0301414E-4	45.0	26
TTCGTAG	35	1.2104101E-7	45.0	1
TATTGCG	20	7.0301414E-4	45.0	1
GCCGATT	25	3.8881975E-5	45.0	9
GCCTATT	20	7.0301414E-4	45.0	43
TTACGTA	20	7.0301414E-4	45.0	21
TATAGCG	40	6.8048394E-9	45.0	1
TTACGCG	20	7.0301414E-4	45.0	1
TGTAACG	20	7.0301414E-4	45.0	1
ATCGATG	25	3.8881975E-5	45.0	1
AATCGCG	25	3.8881975E-5	45.0	1
TACGGCT	780	0.0	42.115383	7
CGTTTTT	1755	0.0	42.05128	1
CCGATGA	585	0.0	41.153847	18
CGATGAA	585	0.0	41.153847	19
TTATGCG	45	1.9252184E-8	40.0	1
ACGGCTG	820	0.0	39.786587	8
>>END_MODULE