##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351890
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.124621330529425	31.0	31.0	33.0	28.0	34.0
2	31.027042541703374	31.0	31.0	34.0	28.0	34.0
3	30.869157975503708	31.0	30.0	34.0	27.0	34.0
4	34.71396174941033	35.0	35.0	37.0	32.0	37.0
5	34.550305493193896	35.0	35.0	37.0	32.0	37.0
6	34.753880474011766	36.0	35.0	37.0	32.0	37.0
7	35.32925345988804	37.0	35.0	37.0	33.0	37.0
8	34.8174827360823	37.0	35.0	37.0	32.0	37.0
9	36.84164937906732	39.0	37.0	39.0	32.0	39.0
10	36.371073346784506	37.0	35.0	39.0	32.0	39.0
11	36.67093694052119	38.0	35.0	39.0	32.0	39.0
12	36.76608883457899	39.0	35.0	39.0	33.0	39.0
13	36.821313478643894	39.0	37.0	39.0	33.0	39.0
14	37.7535849271079	39.0	37.0	41.0	33.0	41.0
15	37.93983631248401	40.0	37.0	41.0	33.0	41.0
16	37.922927619426524	39.0	37.0	41.0	33.0	41.0
17	37.77655801528887	39.0	36.0	41.0	33.0	41.0
18	37.77946801557305	39.0	36.0	41.0	33.0	41.0
19	37.69478530222513	39.0	36.0	41.0	33.0	41.0
20	37.76437238909887	39.0	36.0	41.0	33.0	41.0
21	37.46848162778141	39.0	35.0	41.0	33.0	41.0
22	37.65636704652022	39.0	36.0	41.0	33.0	41.0
23	37.74775355935093	39.0	36.0	41.0	33.0	41.0
24	37.65636704652022	39.0	36.0	41.0	33.0	41.0
25	37.15573332575521	39.0	35.0	40.0	32.0	41.0
26	37.3610787461991	39.0	35.0	41.0	33.0	41.0
27	37.48930631731507	39.0	35.0	41.0	33.0	41.0
28	37.408283838699596	39.0	35.0	41.0	33.0	41.0
29	37.475370144079115	39.0	35.0	41.0	33.0	41.0
30	37.07088578817244	39.0	35.0	41.0	32.0	41.0
31	37.08936883685242	39.0	35.0	41.0	32.0	41.0
32	37.07427889397255	39.0	35.0	41.0	32.0	41.0
33	37.0627184631561	39.0	35.0	41.0	32.0	41.0
34	36.93190769842849	39.0	35.0	41.0	31.0	41.0
35	36.91148370229333	39.0	35.0	41.0	31.0	41.0
36	36.67782829861604	39.0	35.0	41.0	31.0	41.0
37	36.73810849981528	39.0	35.0	41.0	31.0	41.0
38	36.4584330330501	39.0	35.0	41.0	30.0	41.0
39	36.42367785387479	39.0	35.0	40.0	30.0	41.0
40	36.31098354599449	39.0	35.0	40.0	30.0	41.0
41	36.11385660291568	39.0	35.0	40.0	30.0	41.0
42	36.12504475830515	39.0	35.0	40.0	30.0	41.0
43	36.04222910568644	38.0	35.0	40.0	30.0	41.0
44	35.99460058541021	38.0	35.0	40.0	30.0	41.0
45	35.975264997584475	38.0	35.0	40.0	30.0	41.0
46	35.7831339338998	38.0	35.0	40.0	30.0	41.0
47	35.68844809457501	37.0	35.0	40.0	29.0	41.0
48	35.59601295859502	37.0	34.0	40.0	29.0	41.0
49	35.57533888431044	37.0	34.0	40.0	29.0	41.0
50	35.46513114893859	37.0	34.0	40.0	29.0	41.0
51	34.6153996987695	36.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	24.0
10	19.0
11	17.0
12	20.0
13	22.0
14	23.0
15	25.0
16	45.0
17	77.0
18	120.0
19	202.0
20	339.0
21	580.0
22	849.0
23	1225.0
24	1720.0
25	2453.0
26	3508.0
27	4016.0
28	4483.0
29	5132.0
30	6183.0
31	7786.0
32	10326.0
33	15168.0
34	25609.0
35	29492.0
36	34068.0
37	51943.0
38	75087.0
39	71313.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.595100741709054	22.464974850095203	22.373468981784082	13.566455426411665
2	32.95688993719628	25.27352297592998	24.60086958992867	17.168717496945067
3	33.57668589616073	23.68723180539373	26.82059734576146	15.915484952684078
4	30.74369831481429	24.917161613004062	26.12719884054676	18.21194123163489
5	25.83506209326778	30.629173889567763	24.329193782147833	19.206570235016624
6	25.824831623518712	34.66282076785359	24.897837392366934	14.614510216260761
7	74.94216942794624	7.750717553781011	12.289920145500014	5.017192872772742
8	75.28290090653329	8.02353008042286	10.936940521185598	5.756628491858251
9	67.61289039188382	10.572337946517377	13.97340077865242	7.841370882946375
10	40.192389667225555	24.698343232260083	20.611554747222144	14.497712353292222
11	30.85623348205405	25.445735883372645	24.92256102759385	18.775469606979453
12	28.749893432606783	22.943533490579444	28.896530165676776	19.410042911137005
13	24.801216289181276	25.740714427804146	29.978970701071354	19.479098581943223
14	20.817300861064535	28.462303560771833	28.763534058938873	21.95686151922476
15	21.878143738099975	26.379550427690475	32.342493392821616	19.399812441387933
16	23.09358038023246	28.08377618005627	28.89851942368354	19.924124016027736
17	22.16715450851118	28.430475432663616	27.286367899059368	22.116002159765834
18	21.991531444485492	28.30088948250874	29.011907130069055	20.695671942936713
19	24.430361760777515	28.002500781494216	27.095683310125324	20.471454147602945
20	25.50996049901958	27.956747847338658	28.843388558924666	17.6899030947171
21	24.28486174656853	28.818949103413	28.79564636676234	18.10054278325613
22	23.027366506578762	26.094233993577536	28.573133649720084	22.305265850123618
23	23.815965216402855	26.40256898462588	29.004518457472507	20.776947341498765
24	23.56475034812015	25.479269089772373	29.80903123135071	21.14694933075677
25	21.382534314700617	28.251726391770156	27.946517377589586	22.41922191593964
26	21.54679019011623	28.419108244053536	28.283838699593623	21.750262866236607
27	23.83358435874847	27.821478302878738	28.267924635539515	20.077012702833272
28	20.38222171701384	27.766347438119865	29.221063400494472	22.63036744437182
29	23.33996419335588	26.4054107817784	28.276450026997075	21.978174997868653
30	22.803717070675493	28.596720566085992	27.505754639233853	21.09380772400466
31	24.453948677143426	27.92605643809145	25.109267100514366	22.51072778425076
32	25.482679246355396	29.237830003694338	25.089658700161984	20.189832049788286
33	23.414419278751883	29.71098922958879	24.967177242888404	21.907414248770923
34	20.784336014095313	29.635397425331778	27.21873312682941	22.3615334337435
35	24.539202591719004	28.451220551877004	25.532694876239738	21.476881980164254
36	23.506777686208757	31.964250191821307	24.487197703827903	20.041774418142033
37	22.837534456790472	30.51635454261275	26.435533831595098	20.210577169001674
38	21.812214044161525	30.655034243655688	25.24112648839126	22.291625223791524
39	23.152405581289607	28.70414049845122	26.619398107363097	21.524055812896076
40	22.666174088493563	28.98348915854386	27.11728096848447	21.233055784478104
41	20.308619170763592	30.142658217056468	26.231208616328967	23.317513995850977
42	21.204069453522408	27.354571030719825	27.71832106624229	23.723038449515474
43	23.46301401005996	25.764301344170054	27.52053198442695	23.252152661343032
44	21.830969905368157	26.824575861774985	26.74699479951121	24.597459433345648
45	22.1108869248913	27.355139390150327	25.97402597402597	24.559947710932395
46	22.72045241410668	29.015885646082584	25.809770098610365	22.453891841200374
47	21.977038279007644	26.908977237204805	29.371110290147488	21.74287419364006
48	21.375145642104066	26.9817272443093	29.18809855352525	22.455028560061383
49	22.14783028787405	26.486686180340445	29.320526300832643	22.044957230952853
50	21.05885361902867	26.53272329421126	28.98434169768962	23.424081389070448
51	20.92642587172128	25.6682486004149	26.6566256500611	26.74869987780272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	320.5
2	521.0
3	2190.0
4	3859.0
5	2612.5
6	1366.0
7	1287.0
8	1208.0
9	1192.0
10	1176.0
11	1178.0
12	1180.0
13	1154.5
14	1129.0
15	1114.5
16	1100.0
17	1130.0
18	1160.0
19	1200.5
20	1241.0
21	1701.5
22	2162.0
23	1878.5
24	1595.0
25	2182.0
26	3388.5
27	4008.0
28	4966.0
29	5924.0
30	6858.5
31	7793.0
32	8536.0
33	9279.0
34	10145.0
35	11011.0
36	10637.5
37	10264.0
38	11126.0
39	11988.0
40	13596.5
41	15205.0
42	16601.5
43	17998.0
44	20035.5
45	22073.0
46	25701.5
47	29330.0
48	30663.0
49	31996.0
50	31118.5
51	30241.0
52	26669.0
53	23097.0
54	21058.0
55	19019.0
56	18046.0
57	17073.0
58	15998.0
59	14923.0
60	14351.0
61	13779.0
62	12710.0
63	11641.0
64	10005.5
65	8370.0
66	7111.5
67	5853.0
68	4933.5
69	4014.0
70	3305.5
71	2597.0
72	2191.0
73	1785.0
74	1284.0
75	683.0
76	583.0
77	485.0
78	387.0
79	284.5
80	182.0
81	129.0
82	76.0
83	50.5
84	25.0
85	14.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	351890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.537600184359036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.122829988983	21.3033531637908
2	10.411951660663947	6.150881305831296
3	3.595113722380821	3.185730952469623
4	1.8064773430637764	2.134360220060841
5	1.1422935694995546	1.6870305374521093
6	0.8489356269642799	1.5045312678917444
7	0.7062759712009183	1.4603188080006824
8	0.602528386710297	1.4237794069100498
9	0.5636232922784885	1.4983271515722716
>10	7.998462648966307	51.354756130459336
>50	0.13766373723674816	2.726454402198596
>100	0.05386841891872754	2.9465626307128456
>500	0.007980506506478153	1.54606141233503
>1k	0.0019951266266195382	1.077852610314759
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2211	0.6283213504220069	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	1447	0.411208047969536	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	813	0.2310381084998153	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	737	0.20944045014066895	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	674	0.19153712807979767	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	661	0.18784279178152263	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	655	0.18613771349001107	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	609	0.17306544658842252	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC	564	0.16027735940208587	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	534	0.1517519679445281	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	493	0.14010059961919918	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	490	0.1392480604734434	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	475	0.13498536474466452	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	471	0.13384864588365683	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	468	0.13299610673790105	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	403	0.11452442524652591	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24609963340816732	0.0
2	0.0	0.0	0.0	1.1355821421466936	0.0
3	0.0	0.0	0.0	1.487680809343829	0.0
4	0.0	0.0	0.0	2.00886640711586	0.0
5	0.0	0.0	0.0	3.3774759157691325	0.0
6	0.0	0.0	0.0	4.029384182557049	0.0
7	0.0	0.0	0.0	4.7614311290460085	0.0
8	0.0	0.0	0.0	5.839608968711813	0.0
9	0.0	0.0	0.0	6.232345335189974	0.0
10	0.0	0.0	0.0	7.255108130381653	0.0
11	0.0	0.0	0.0	8.538179544744096	0.0
12	0.0	0.0	0.0	9.65614254454517	0.0
13	0.0	0.0	0.0	10.06933985052147	0.0
14	0.0	0.0	0.0	10.265139674330046	0.0
15	0.0	0.0	0.0	10.615249083520418	0.0
16	0.0	0.0	0.0	11.302679814714825	0.0
17	0.0	0.0	0.0	12.052345903549405	0.0
18	0.0	0.0	0.0	12.871351842905453	0.0
19	0.0	0.0	0.0	13.429196623944982	0.0
20	0.0	0.0	0.0	13.951803120293274	0.0
21	0.0	0.0	0.0	14.561368609508653	0.0
22	0.0	0.0	0.0	15.178606951035835	0.0
23	0.0	0.0	0.0	15.773679274773366	0.0
24	0.0	0.0	0.0	16.258774048708403	0.0
25	0.0	0.0	0.0	16.722839523714796	0.0
26	0.0	0.0	0.0	17.148824916877434	0.0
27	0.0	0.0	0.0	17.560885503992726	0.0
28	0.0	0.0	0.0	17.996248827758674	0.0
29	0.0	0.0	0.0	18.405183438006194	0.0
30	0.0	0.0	0.0	18.870101452158345	0.0
31	0.0	0.0	0.0	19.30887493250732	0.0
32	0.0	0.0	0.0	19.745943334564778	0.0
33	0.0	0.0	0.0	20.151751967944527	0.0
34	0.0	0.0	0.0	20.563528375344568	0.0
35	0.0	0.0	0.0	21.005712012276565	0.0
36	0.0	0.0	0.0	21.447895649208558	0.0
37	0.0	0.0	0.0	21.853135923157804	0.0
38	0.0	0.0	0.0	22.258944556537553	0.0
39	0.0	0.0	0.0	22.728977805564238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	20	7.026607E-4	45.0	11
TCACACG	20	7.026607E-4	45.0	39
TCGGGCA	20	7.026607E-4	45.0	27
AAGGTAC	20	7.026607E-4	45.0	27
TCGATCA	20	7.026607E-4	45.0	17
ACGGGTC	20	7.026607E-4	45.0	5
AGTATAT	20	7.026607E-4	45.0	39
TGTTGCG	20	7.026607E-4	45.0	1
TACGCCC	20	7.026607E-4	45.0	28
CTTGCGT	35	1.2087185E-7	45.0	15
CGGGCCC	20	7.026607E-4	45.0	6
ACGTCGG	20	7.026607E-4	45.0	43
GCGTGCA	20	7.026607E-4	45.0	18
GCTACGA	55	1.8189894E-12	45.0	10
ATCGGTA	20	7.026607E-4	45.0	33
CGATAGA	20	7.026607E-4	45.0	10
AATACGC	20	7.026607E-4	45.0	26
CGCCCCC	20	7.026607E-4	45.0	27
CTATCGG	30	2.161194E-6	44.999996	2
GTTAGCG	25	3.885271E-5	44.999996	1
>>END_MODULE