##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551764_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336559
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33271135224433	31.0	31.0	33.0	28.0	34.0
2	31.29180916273224	31.0	31.0	34.0	28.0	34.0
3	31.11988388365784	31.0	31.0	34.0	28.0	34.0
4	34.94484473747545	35.0	35.0	37.0	32.0	37.0
5	34.519917755876385	35.0	35.0	37.0	32.0	37.0
6	34.8069877792601	37.0	35.0	37.0	32.0	37.0
7	35.46565386752397	37.0	35.0	37.0	33.0	37.0
8	35.121384363514274	37.0	35.0	37.0	32.0	37.0
9	37.0764293927662	39.0	37.0	39.0	33.0	39.0
10	36.51064746448617	38.0	35.0	39.0	32.0	39.0
11	36.736762350731965	39.0	37.0	39.0	33.0	39.0
12	36.82083676264786	39.0	35.0	39.0	33.0	39.0
13	36.880026978924946	39.0	37.0	39.0	33.0	39.0
14	37.83826609896036	40.0	37.0	41.0	33.0	41.0
15	37.994075333002534	40.0	37.0	41.0	33.0	41.0
16	38.0233124058486	40.0	37.0	41.0	33.0	41.0
17	37.880820301938144	40.0	37.0	41.0	33.0	41.0
18	37.8572701963103	39.0	37.0	41.0	33.0	41.0
19	37.77348993787122	39.0	36.0	41.0	33.0	41.0
20	37.84954495348512	39.0	36.0	41.0	33.0	41.0
21	37.63285783473329	39.0	36.0	41.0	33.0	41.0
22	37.801975285165454	39.0	36.0	41.0	33.0	41.0
23	37.861655757237216	40.0	36.0	41.0	33.0	41.0
24	37.79612192810176	40.0	36.0	41.0	33.0	41.0
25	37.334048413502536	39.0	35.0	41.0	32.0	41.0
26	37.51979593473953	39.0	35.0	41.0	33.0	41.0
27	37.621219459292426	39.0	36.0	41.0	33.0	41.0
28	37.52901868617389	39.0	35.0	41.0	33.0	41.0
29	37.59704836299133	40.0	36.0	41.0	33.0	41.0
30	37.25542624027288	39.0	35.0	41.0	32.0	41.0
31	37.260055443473505	39.0	35.0	41.0	33.0	41.0
32	37.29396925947605	39.0	35.0	41.0	33.0	41.0
33	37.320579749761556	39.0	35.0	41.0	33.0	41.0
34	37.190320270740045	39.0	35.0	41.0	32.0	41.0
35	37.17161329811415	40.0	35.0	41.0	32.0	41.0
36	37.00741326186493	39.0	35.0	41.0	32.0	41.0
37	37.030333463077795	39.0	35.0	41.0	32.0	41.0
38	36.84300523830888	39.0	35.0	41.0	31.0	41.0
39	36.84815440977659	39.0	35.0	41.0	31.0	41.0
40	36.751199641073335	39.0	35.0	41.0	31.0	41.0
41	36.5858081346807	39.0	35.0	41.0	31.0	41.0
42	36.68806360846092	39.0	35.0	41.0	31.0	41.0
43	36.650177234897896	39.0	35.0	41.0	31.0	41.0
44	36.678710122147976	39.0	35.0	41.0	31.0	41.0
45	36.68458724918959	39.0	35.0	41.0	31.0	41.0
46	36.52425874809469	39.0	35.0	41.0	31.0	41.0
47	36.43526989324309	39.0	35.0	41.0	31.0	41.0
48	36.39229971565164	39.0	35.0	40.0	31.0	41.0
49	36.391152814216824	39.0	35.0	40.0	31.0	41.0
50	36.33931346361262	38.0	35.0	40.0	31.0	41.0
51	35.42043148452426	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	20.0
10	26.0
11	18.0
12	18.0
13	19.0
14	21.0
15	38.0
16	41.0
17	64.0
18	109.0
19	189.0
20	309.0
21	550.0
22	742.0
23	1156.0
24	1507.0
25	2052.0
26	2694.0
27	3127.0
28	3482.0
29	4030.0
30	4925.0
31	6431.0
32	9039.0
33	13300.0
34	24371.0
35	26222.0
36	28510.0
37	44628.0
38	77066.0
39	81824.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93321230452907	22.81293918748273	21.831239099236686	13.422609408751512
2	33.1294067310635	25.32661435290692	24.164559557165312	17.379419358864272
3	32.663515163760295	24.681259452280283	26.064672167435727	16.590553216523702
4	28.773558276557747	25.638298188430557	26.617027029436148	18.971116505575544
5	26.700816201616952	29.88450761976355	23.645779788982022	19.768896389637476
6	26.95872046208837	33.46515766923481	24.43553730549473	15.140584563182086
7	77.11099688316165	6.848724889246759	11.230423194744459	4.809855032847139
8	77.62086290962358	7.187744199382575	9.821457753321111	5.369935137672741
9	70.92040325767547	8.739032383623673	11.889742957401229	8.450821401299624
10	42.48734991487377	23.14839300093	19.586461809073594	14.777795275122637
11	32.026479755406925	25.14447689706708	24.23111549535148	18.597927852174507
12	29.639379722426085	22.004462813355165	28.394427128675805	19.961730335542953
13	26.909100633172788	22.732121262542375	29.500028226848784	20.85874987743605
14	21.555210230598497	26.751030279980625	28.898350660656824	22.79540882876405
15	20.476647482313652	25.369697437893507	32.962422636149974	21.191232443642868
16	23.39322377354342	24.91806785734448	30.671591013759848	21.017117355352255
17	23.116897780181187	25.72357298423159	28.76375316066425	22.39577607492297
18	23.38044741040947	26.012081091279686	28.790197261104293	21.81727423720655
19	24.365712995344055	27.3940081828149	26.86393767511788	21.37634114672316
20	26.819963215959163	26.459848050416124	28.202187432218423	18.518001301406294
21	25.005125401489785	27.21543622366361	27.93655792892182	19.842880445924784
22	23.967862989847248	24.704732305479872	28.318957448768266	23.00844725590461
23	23.908140920314118	25.91878392792943	29.080190991772614	21.092884159983836
24	24.652735478771923	24.698789810998964	28.799111002825654	21.84936370740346
25	22.091817482224513	27.432337272216756	27.652209568010367	22.823635677548364
26	22.225820732768995	28.299644341705317	27.627548215914594	21.846986709611095
27	22.98527152742907	28.474056554719972	27.11292819386794	21.427743723983017
28	20.080877349885164	28.723344198194077	29.070980125327207	22.124798326593552
29	22.185708895022863	27.714308635335854	28.403043745673124	21.69693872396816
30	23.14334188062123	28.12463787924257	27.574660015034514	21.15736022510169
31	23.196230081501312	28.49990640571193	25.45348661007431	22.85037690271245
32	25.159036008545304	28.106810395799847	26.538883227012207	20.195270368642646
33	25.41396902177627	27.1028259532504	25.90511619062334	21.578088834349995
34	23.0966932989461	26.54482572149311	27.631707962051234	22.72677301750956
35	23.81365525806768	26.742116538259264	27.183049628742655	22.261178574930398
36	24.016591444590695	30.515006284187912	24.71780579333787	20.75059647788352
37	22.682204308902747	29.312542525976127	27.808200048134207	20.19705311698692
38	23.69955936403424	28.318066074596132	25.77497556149145	22.207398999878176
39	25.52241954605285	24.941540710544068	26.773314634284034	22.762725109119057
40	25.75982220056513	24.385026102407007	27.625468342846276	22.229683354181585
41	22.461440638937006	26.907912134276607	26.64971074908114	23.980936477705246
42	23.832671240406587	26.46638479434512	26.759944021701994	22.940999943546302
43	23.95746362450566	26.675857724797137	27.02468215082645	22.34199649987075
44	23.16057511461586	26.66159573804296	26.477378409134804	23.700450738206378
45	23.752150440190277	24.971847432396697	26.281573215988875	24.994428911424148
46	23.485926687445588	27.08024447422294	26.293755329674738	23.140073508656727
47	21.826187978927912	27.014282785484863	28.959855478534223	22.199673757053
48	23.02092649431452	25.396141538333545	28.326979816317493	23.25595215103444
49	22.953182057232162	24.53953095891062	29.486360489542697	23.02092649431452
50	22.167584286856094	24.757620506359952	28.845462459776737	24.229332747007213
51	21.12378513128456	24.59152778561857	27.062714115504267	27.221972967592606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	318.0
2	516.0
3	1412.0
4	2308.0
5	1595.0
6	882.0
7	891.0
8	900.0
9	894.0
10	888.0
11	867.0
12	846.0
13	813.0
14	780.0
15	831.0
16	882.0
17	858.0
18	834.0
19	848.5
20	863.0
21	1217.0
22	1571.0
23	1515.0
24	1459.0
25	1768.0
26	2536.5
27	2996.0
28	3493.5
29	3991.0
30	4818.0
31	5645.0
32	6303.5
33	6962.0
34	8062.0
35	9162.0
36	9782.0
37	10402.0
38	11467.5
39	12533.0
40	13488.5
41	14444.0
42	15575.5
43	16707.0
44	19095.5
45	21484.0
46	24903.5
47	28323.0
48	28717.5
49	29112.0
50	28886.5
51	28661.0
52	25960.5
53	23260.0
54	21496.5
55	19733.0
56	18573.0
57	17413.0
58	17246.5
59	17080.0
60	16197.0
61	15314.0
62	14044.0
63	12774.0
64	10882.5
65	8991.0
66	7211.0
67	5431.0
68	4837.5
69	4244.0
70	3565.0
71	2886.0
72	2329.5
73	1773.0
74	1412.0
75	835.0
76	619.0
77	486.0
78	353.0
79	277.5
80	202.0
81	131.5
82	61.0
83	40.5
84	20.0
85	10.5
86	1.0
87	1.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	336559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.097354715981606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03710662964463	21.779687481430862
2	9.634882556282506	5.992387209990815
3	3.4082509344102765	3.179627648052964
4	1.9479025551635911	2.4229846684035654
5	1.480906951386915	2.3026144384320917
6	1.2603768874310524	2.3516632286560957
7	1.074890129083973	2.339836773738209
8	0.956230456809344	2.378899016450024
9	0.9113599398739455	2.550679499177618
>10	9.182399148656069	49.565223066278485
>50	0.07278913416805322	1.5376633004045508
>100	0.02592489710095046	1.4492918463583122
>500	0.002991334280878899	0.6982895246039621
>1k	0.003988445707838533	1.4511522980224436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC	1230	0.3654634105758574	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG	1190	0.3535784216140409	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1166	0.346447428236951	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC	1094	0.3250544481056813	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	995	0.2956391004251855	No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	727	0.21600967438101493	No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	530	0.15747610374406865	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC	465	0.13816299668111684	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17381796356656634	0.0
2	0.0	0.0	0.0	0.8961281677209643	0.0
3	0.0	0.0	0.0	1.1786937802881516	0.0
4	0.0	0.0	0.0	1.6439910981432675	0.0
5	0.0	0.0	0.0	2.989668973344941	0.0
6	0.0	0.0	0.0	3.5714391830258587	0.0
7	0.0	0.0	0.0	4.215902709480359	0.0
8	0.0	0.0	0.0	5.064788046078102	0.0
9	0.0	0.0	0.0	5.3827115008066935	0.0
10	0.0	0.0	0.0	6.45681737823086	0.0
11	0.0	0.0	0.0	7.65957826116669	0.0
12	0.0	0.0	0.0	8.920278465291375	0.0
13	0.0	0.0	0.0	9.333578956438544	0.0
14	0.0	0.0	0.0	9.521361782035244	0.0
15	0.0	0.0	0.0	9.886528067887056	0.0
16	0.0	0.0	0.0	10.473646522600792	0.0
17	0.0	0.0	0.0	11.139503029186562	0.0
18	0.0	0.0	0.0	11.839231754313507	0.0
19	0.0	0.0	0.0	12.374947631767387	0.0
20	0.0	0.0	0.0	12.822714590903823	0.0
21	0.0	0.0	0.0	13.372098205663791	0.0
22	0.0	0.0	0.0	13.941983426382892	0.0
23	0.0	0.0	0.0	14.467002813771137	0.0
24	0.0	0.0	0.0	14.883571676882806	0.0
25	0.0	0.0	0.0	15.281124557655566	0.0
26	0.0	0.0	0.0	15.64629084350738	0.0
27	0.0	0.0	0.0	15.985904403091286	0.0
28	0.0	0.0	0.0	16.359093056492323	0.0
29	0.0	0.0	0.0	16.751297692232267	0.0
30	0.0	0.0	0.0	17.156575815830212	0.0
31	0.0	0.0	0.0	17.53748971205643	0.0
32	0.0	0.0	0.0	17.938013840069647	0.0
33	0.0	0.0	0.0	18.30288300119741	0.0
34	0.0	0.0	0.0	18.653490175571	0.0
35	0.0	0.0	0.0	19.041237940450262	0.0
36	0.0	0.0	0.0	19.41977483888412	0.0
37	0.0	0.0	0.0	19.792369242837065	0.0
38	0.0	0.0	0.0	20.18338537968083	0.0
39	0.0	0.0	0.0	20.595794496655863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	20	7.0261705E-4	45.0	17
ATTAGTC	40	6.7902874E-9	45.0	31
GACCGTT	20	7.0261705E-4	45.0	9
TTCCGAA	20	7.0261705E-4	45.0	45
TACGAAT	25	3.8849077E-5	45.0	12
CGAATAT	25	3.8849077E-5	45.0	14
ATCGAGT	20	7.0261705E-4	45.0	23
CGAGGGT	30	2.1609121E-6	44.999996	4
ACGGGTA	80	0.0	42.1875	5
CATGCGG	45	1.9213985E-8	40.0	2
ACGGGAC	85	0.0	39.705883	5
CGTTTTT	680	0.0	39.705883	1
ACGGGTC	40	3.448513E-7	39.375	5
CACGGGT	35	6.23513E-6	38.571426	4
GCGCGAC	35	6.23513E-6	38.571426	9
TCGTCCC	35	6.23513E-6	38.571426	38
TACGGGA	95	0.0	37.894737	4
TACGGCT	190	0.0	37.894737	7
AACCCCG	30	1.1382075E-4	37.499996	19
CTACGAA	30	1.1382075E-4	37.499996	11
>>END_MODULE