FastQCFastQC Report
Sat 18 Jun 2016
SRR3551763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350232
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.44713218666483934No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC10780.30779597523927TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC7380.2107174672788323TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT7130.203579341693506TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC4770.13619543616802576TruSeq Adapter, Index 22 (95% over 23bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4340.12391786016126453No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4070.11620868452911212No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCC3920.11192580917791636No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3880.11078370908426416No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3790.10821398387354667No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA253.885232E-545.09
CGATTAC207.026563E-445.012
AGGCGGT253.885232E-545.03
GCGTTGG253.885232E-545.02
GTAATCT207.026563E-445.030
TATTGCG351.2087003E-745.01
GCGATAT207.026563E-445.09
TAGTGCG207.026563E-445.01
CGTTTTT9550.040.0523571
AATAGCG451.9217623E-840.0000041
CGTTTGG451.9217623E-840.0000042
CGAGGGC850.039.7058834
AATACGG403.4490404E-739.3752
ACGACAG403.4490404E-739.3751
CGAAAGG356.235856E-638.5714262
ATAGCGG700.038.5714262
TAAGTAG356.235856E-638.5714261
TAGCATA356.235856E-638.57142630
CGCACGG356.235856E-638.5714262
GCGTATG356.235856E-638.5714261
Produced by FastQC (version 0.11.5)