##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551763_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350232
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.304406793211356	31.0	31.0	33.0	28.0	34.0
2	31.227620548664884	31.0	31.0	34.0	28.0	34.0
3	31.064474405536902	31.0	31.0	34.0	27.0	34.0
4	34.88390267023002	35.0	35.0	37.0	32.0	37.0
5	34.53351778249846	35.0	35.0	37.0	32.0	37.0
6	34.82008211699674	36.0	35.0	37.0	32.0	37.0
7	35.48200050252404	37.0	35.0	37.0	33.0	37.0
8	35.13982731446584	37.0	35.0	37.0	32.0	37.0
9	37.16589289385322	39.0	37.0	39.0	34.0	39.0
10	36.59216176705726	38.0	35.0	39.0	32.0	39.0
11	36.81633031818909	39.0	37.0	39.0	33.0	39.0
12	36.919984467438724	39.0	37.0	39.0	33.0	39.0
13	37.01893316430252	39.0	37.0	39.0	33.0	39.0
14	37.984707279746	40.0	37.0	41.0	33.0	41.0
15	38.18007206651591	40.0	37.0	41.0	34.0	41.0
16	38.14817035564997	40.0	37.0	41.0	34.0	41.0
17	38.029509011169736	40.0	37.0	41.0	33.0	41.0
18	38.00773201763403	39.0	37.0	41.0	34.0	41.0
19	37.94339180885813	39.0	37.0	41.0	34.0	41.0
20	38.034736974348434	40.0	37.0	41.0	34.0	41.0
21	37.75629011626579	39.0	36.0	41.0	33.0	41.0
22	37.92520386486672	40.0	36.0	41.0	33.0	41.0
23	38.05200267251422	40.0	37.0	41.0	34.0	41.0
24	37.94917654583248	40.0	36.0	41.0	34.0	41.0
25	37.473548961831014	39.0	35.0	41.0	33.0	41.0
26	37.67968375248407	39.0	36.0	41.0	33.0	41.0
27	37.824619109618766	40.0	36.0	41.0	34.0	41.0
28	37.74375271248772	40.0	36.0	41.0	33.0	41.0
29	37.78961945224879	40.0	36.0	41.0	33.0	41.0
30	37.4293182804541	39.0	36.0	41.0	33.0	41.0
31	37.46749868658489	39.0	36.0	41.0	33.0	41.0
32	37.50487962265013	39.0	36.0	41.0	33.0	41.0
33	37.54596381826903	40.0	36.0	41.0	33.0	41.0
34	37.415498869320906	40.0	36.0	41.0	33.0	41.0
35	37.38363142145778	40.0	36.0	41.0	33.0	41.0
36	37.202899792137785	40.0	35.0	41.0	33.0	41.0
37	37.295033006692705	40.0	36.0	41.0	33.0	41.0
38	37.0556488270632	39.0	35.0	41.0	32.0	41.0
39	37.08674535736312	39.0	35.0	41.0	32.0	41.0
40	37.012269010256055	39.0	35.0	41.0	32.0	41.0
41	36.869280933781035	39.0	35.0	41.0	31.0	41.0
42	36.990146531442015	39.0	35.0	41.0	32.0	41.0
43	36.98499280476941	39.0	35.0	41.0	32.0	41.0
44	36.9820804495306	39.0	35.0	41.0	32.0	41.0
45	37.021225930240526	39.0	35.0	41.0	32.0	41.0
46	36.868444345462436	39.0	35.0	41.0	32.0	41.0
47	36.80040087713287	39.0	35.0	41.0	32.0	41.0
48	36.74480915507435	39.0	35.0	41.0	32.0	41.0
49	36.78389182027913	39.0	35.0	41.0	32.0	41.0
50	36.741879668334136	39.0	35.0	41.0	32.0	41.0
51	35.85219797163023	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	20.0
10	19.0
11	21.0
12	21.0
13	15.0
14	18.0
15	28.0
16	42.0
17	66.0
18	98.0
19	158.0
20	254.0
21	407.0
22	575.0
23	904.0
24	1329.0
25	1990.0
26	2781.0
27	3187.0
28	3437.0
29	3945.0
30	4819.0
31	6101.0
32	8370.0
33	12658.0
34	22836.0
35	25940.0
36	29375.0
37	46463.0
38	83097.0
39	91235.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.89821603965372	22.963064482971287	25.373752255647688	12.76496722172731
2	33.22569039950661	23.751399072614724	26.000194157015923	17.022716370862742
3	33.36274241074488	22.689531510541585	27.867813335160697	16.079912743552846
4	29.754848214897557	25.869709221316157	25.499954315996256	18.875488247790038
5	25.450272961922384	31.080826423627766	24.966022522213844	18.502878092236003
6	26.279437629913886	33.664827885515884	24.57057036478677	15.485164119783457
7	77.9292012151945	7.104719157586971	10.056191324607688	4.909888302610841
8	79.08700518513443	6.087107974142854	9.736117773361658	5.089769067361064
9	71.57227209392632	9.8354804815094	12.128246419516206	6.4640010050480825
10	39.01471024920624	29.482171817538088	18.331848603211586	13.171269330044085
11	29.215776970693714	25.97678110509605	26.702014664565205	18.105427259645033
12	26.87018890335549	23.829918454053313	30.152299047488523	19.147593595102673
13	24.49547728362914	25.52193974279906	30.723063569291213	19.259519404280592
14	20.236586034400055	27.105175997624432	30.510061901825075	22.148176066150437
15	20.35336576897599	27.50548208044953	32.366260079033324	19.77489207154115
16	22.874266200689828	27.418396948308548	30.161435848237737	19.545901002763884
17	23.014458987185638	27.6054158386441	27.789293953722105	21.59083122044816
18	21.965154526142673	27.655953767788212	30.06721259051143	20.311679115557688
19	23.765389798761962	27.896936887548822	27.963464218004063	20.374209095685146
20	24.213664085520456	28.716679229767696	28.42887000616734	18.640786678544508
21	24.079752849539734	28.187030311336486	28.947383448685443	18.785833390438338
22	22.88568720162635	25.842013294045092	29.484456017725392	21.78784348660317
23	22.635281756093104	25.31122227552023	30.440679321135704	21.612816647250966
24	21.86122341762032	26.283434980241672	30.6331231869161	21.22221841522191
25	20.93498024166838	28.6884122524498	28.214155188560724	22.16245231732109
26	20.02958039242559	29.342550081089108	28.65729002489778	21.97057950158752
27	22.193003494826286	27.752175700678407	29.820804495305968	20.234016309189336
28	19.23696292743096	28.49054341122456	30.66281778935106	21.60967587199342
29	20.050138194111334	27.855821284177345	29.44105621416661	22.652984307544713
30	21.79498161218849	27.79928732954156	29.57011352474931	20.835617533520637
31	22.176443043468332	27.3844194705224	29.08300783480664	21.356129651202632
32	22.009410904771695	27.504911030402706	27.966033943214786	22.519644121610817
33	22.081648735695197	27.20054135544439	28.19702368715594	22.52078622170447
34	20.218597957925034	27.142579775691544	30.14458987185637	22.494232394527057
35	21.388393978848306	25.945658877544027	28.762077708490374	23.903869435117294
36	21.507172388588135	28.716393704744288	28.267262842915553	21.509171063752028
37	20.214029557550422	27.83669110760867	30.624557436213713	21.324721898627196
38	20.409328673564954	27.804141254939584	27.842401608076933	23.944128463418537
39	23.293416935060186	25.501096416089908	28.15476598369081	23.050720665159094
40	22.53392037278147	26.12354096713036	29.17094954201786	22.171589118070308
41	19.96105438680646	26.430480367299392	28.288106169624704	25.320359076269444
42	19.65811233696521	26.238893076589232	29.034754105849835	25.068240480595723
43	20.994083921514882	25.37774960597547	28.7123963544165	24.91577011809315
44	20.77879805386144	26.217764224856666	28.193026336828158	24.810411384453733
45	22.142465565682176	25.55191987025743	27.777872952785582	24.527741611274813
46	21.903766646108867	26.901311130907512	28.257840517142924	22.9370817058407
47	20.94440256744101	24.688777724479774	30.853262979967568	23.513556728111652
48	19.920509833481805	25.023984101966697	30.509205326754834	24.54630073779666
49	21.047191575869707	24.790139107791408	31.4271682770278	22.735501039311085
50	20.564083236254827	23.893019484227597	30.68366111606021	24.859236163457364
51	20.385915621645083	24.052056922268665	28.595045569793736	26.966981886292512
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	305.5
2	480.0
3	1659.5
4	2839.0
5	1982.0
6	1125.0
7	1086.0
8	1047.0
9	1025.5
10	1004.0
11	995.0
12	986.0
13	914.5
14	843.0
15	866.0
16	889.0
17	918.5
18	948.0
19	1053.0
20	1158.0
21	1410.0
22	1662.0
23	1680.5
24	1699.0
25	2379.0
26	3820.0
27	4581.0
28	5359.0
29	6137.0
30	6808.5
31	7480.0
32	8171.5
33	8863.0
34	9784.5
35	10706.0
36	11685.5
37	12665.0
38	13404.5
39	14144.0
40	15655.0
41	17166.0
42	19052.5
43	20939.0
44	22506.0
45	24073.0
46	27199.0
47	30325.0
48	31882.5
49	33440.0
50	32095.0
51	30750.0
52	26486.0
53	22222.0
54	20251.0
55	18280.0
56	16908.0
57	15536.0
58	14609.5
59	13683.0
60	12750.5
61	11818.0
62	10330.5
63	8843.0
64	7747.0
65	6651.0
66	5807.0
67	4963.0
68	4141.0
69	3319.0
70	2906.0
71	2493.0
72	2032.5
73	1572.0
74	1158.5
75	618.5
76	492.0
77	392.0
78	292.0
79	199.0
80	106.0
81	79.5
82	53.0
83	33.0
84	13.0
85	10.0
86	7.0
87	4.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	350232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.147689513966004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4180993896637	20.92795307049214
2	10.770460309767188	6.494089866827106
3	3.816224818903829	3.4515108286741127
4	1.9681764418301642	2.373438891079926
5	1.380336841282431	2.080698325783565
6	1.0464745224564604	1.8929273392375927
7	0.9080341692465923	1.9162592541762824
8	0.88711812607536	2.1395649461705037
9	0.695953504562985	1.8883251154549252
>10	8.963758253382203	51.151048785959865
>50	0.09856848397316065	2.071423713310953
>100	0.04281257384692836	2.3835930600495985
>500	0.001991282504508296	0.43553627126709066
>1k	0.001991282504508296	0.7936305315163251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1566	0.44713218666483934	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1078	0.30779597523927	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	738	0.2107174672788323	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	713	0.203579341693506	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	477	0.13619543616802576	TruSeq Adapter, Index 22 (95% over 23bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	434	0.12391786016126453	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	407	0.11620868452911212	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCC	392	0.11192580917791636	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	388	0.11078370908426416	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	379	0.10821398387354667	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20928984216176705	0.0
2	0.0	0.0	0.0	0.9653601041595286	0.0
3	0.0	0.0	0.0	1.2782955298202334	0.0
4	0.0	0.0	0.0	1.7508394435688344	0.0
5	0.0	0.0	0.0	3.0516914502386987	0.0
6	0.0	0.0	0.0	3.724959455446675	0.0
7	0.0	0.0	0.0	4.4219260375979355	0.0
8	0.0	0.0	0.0	5.44125037118253	0.0
9	0.0	0.0	0.0	5.802154000776628	0.0
10	0.0	0.0	0.0	6.64902122021974	0.0
11	0.0	0.0	0.0	7.962721852943192	0.0
12	0.0	0.0	0.0	8.945784508554329	0.0
13	0.0	0.0	0.0	9.340094565887755	0.0
14	0.0	0.0	0.0	9.509125379748282	0.0
15	0.0	0.0	0.0	9.838050206720117	0.0
16	0.0	0.0	0.0	10.507320861600311	0.0
17	0.0	0.0	0.0	11.285947600447702	0.0
18	0.0	0.0	0.0	12.158226546974577	0.0
19	0.0	0.0	0.0	12.704721441787159	0.0
20	0.0	0.0	0.0	13.246933461248544	0.0
21	0.0	0.0	0.0	13.891648964115214	0.0
22	0.0	0.0	0.0	14.52265926585806	0.0
23	0.0	0.0	0.0	15.131969665821513	0.0
24	0.0	0.0	0.0	15.660190959135658	0.0
25	0.0	0.0	0.0	16.132163822837434	0.0
26	0.0	0.0	0.0	16.549315882043903	0.0
27	0.0	0.0	0.0	16.97760341716348	0.0
28	0.0	0.0	0.0	17.415884328102514	0.0
29	0.0	0.0	0.0	17.886429566687223	0.0
30	0.0	0.0	0.0	18.383528632449348	0.0
31	0.0	0.0	0.0	18.8680645971813	0.0
32	0.0	0.0	0.0	19.314340208775896	0.0
33	0.0	0.0	0.0	19.749194819433974	0.0
34	0.0	0.0	0.0	20.194613855958337	0.0
35	0.0	0.0	0.0	20.657735443934307	0.0
36	0.0	0.0	0.0	21.116574156559082	0.0
37	0.0	0.0	0.0	21.562278718106853	0.0
38	0.0	0.0	0.0	22.043959432604673	0.0
39	0.0	0.0	0.0	22.514790196212797	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTA	25	3.885232E-5	45.0	9
CGATTAC	20	7.026563E-4	45.0	12
AGGCGGT	25	3.885232E-5	45.0	3
GCGTTGG	25	3.885232E-5	45.0	2
GTAATCT	20	7.026563E-4	45.0	30
TATTGCG	35	1.2087003E-7	45.0	1
GCGATAT	20	7.026563E-4	45.0	9
TAGTGCG	20	7.026563E-4	45.0	1
CGTTTTT	955	0.0	40.052357	1
AATAGCG	45	1.9217623E-8	40.000004	1
CGTTTGG	45	1.9217623E-8	40.000004	2
CGAGGGC	85	0.0	39.705883	4
AATACGG	40	3.4490404E-7	39.375	2
ACGACAG	40	3.4490404E-7	39.375	1
CGAAAGG	35	6.235856E-6	38.571426	2
ATAGCGG	70	0.0	38.571426	2
TAAGTAG	35	6.235856E-6	38.571426	1
TAGCATA	35	6.235856E-6	38.571426	30
CGCACGG	35	6.235856E-6	38.571426	2
GCGTATG	35	6.235856E-6	38.571426	1
>>END_MODULE