SRR3551756_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743255
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	26698	3.5920377259486984	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC	20428	2.7484510699558027	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG	20119	2.7068771821245736	No Hit
GAACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT	7956	1.070426704159407	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	6611	0.889465930266194	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5820	0.7830421591513007	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	4715	0.6343717835736052	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCTGTGT	4270	0.5745000033635831	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC	3791	0.5100537500588628	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA	3717	0.5000975439115781	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT	2865	0.38546662989149083	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1193	0.16051018829338518	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG	1190	0.1601065583144412	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	1158	0.1558011718723722	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGT	888	0.11947447376741496	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTAT	869	0.11691815056743649	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATGCTGTG	762	0.10252201465176825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCC	757	0.10184929802019495	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.1644628E-6	45.000004	1
GCGACGA	20	7.031675E-4	45.000004	33
TACGCTT	20	7.031675E-4	45.000004	29
TTACGCG	20	7.031675E-4	45.000004	1
GACGATT	20	7.031675E-4	45.000004	27
CCGCAAT	20	7.031675E-4	45.000004	42
CGCGCAT	90	0.0	45.0	41
TCGATAG	25	3.889471E-5	45.0	1
CCGCGTA	105	0.0	45.0	40
CGGTCGA	165	0.0	45.0	41
CCCGGTC	105	0.0	45.0	39
GCGAACC	135	0.0	43.333332	33
CCGATGA	3115	0.0	43.12199	18
GATACCT	2380	0.0	43.014706	5
CCCGTCG	225	0.0	43.0	40
CGATGAA	3185	0.0	42.88069	19
ACGGCTG	2135	0.0	42.786884	8
GATGAAT	3405	0.0	42.753304	20
CGTTTTT	3390	0.0	42.67699	1
ACGGCTA	95	0.0	42.63158	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content