##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743255
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08397925341908	31.0	30.0	33.0	28.0	34.0
2	30.93993582283335	31.0	30.0	34.0	28.0	34.0
3	30.837211993192106	31.0	30.0	34.0	27.0	34.0
4	34.81512805161082	35.0	35.0	37.0	32.0	37.0
5	34.497074355369286	35.0	35.0	37.0	32.0	37.0
6	34.642134933501964	35.0	35.0	37.0	32.0	37.0
7	35.13642827831632	35.0	35.0	37.0	32.0	37.0
8	34.53501960968981	35.0	35.0	37.0	31.0	37.0
9	36.25681630126942	39.0	35.0	39.0	32.0	39.0
10	35.9962543137954	37.0	35.0	39.0	32.0	39.0
11	36.382736745800564	37.0	35.0	39.0	32.0	39.0
12	36.49842247949896	38.0	35.0	39.0	32.0	39.0
13	36.42676335847051	39.0	35.0	39.0	32.0	39.0
14	37.43097591001742	39.0	36.0	41.0	32.0	41.0
15	37.34882375496969	39.0	36.0	41.0	31.0	41.0
16	37.66424578374851	39.0	36.0	41.0	33.0	41.0
17	37.27201431540992	39.0	36.0	41.0	31.0	41.0
18	37.27828537984944	39.0	36.0	40.0	32.0	41.0
19	37.22913939361323	39.0	36.0	40.0	32.0	41.0
20	36.79617358779961	39.0	35.0	40.0	30.0	41.0
21	37.029173029444806	39.0	35.0	40.0	31.0	41.0
22	37.08893448412725	39.0	35.0	40.0	31.0	41.0
23	37.01626225185165	39.0	35.0	40.0	31.0	41.0
24	36.95686002785047	39.0	35.0	41.0	31.0	41.0
25	36.84262870750953	39.0	35.0	40.0	31.0	41.0
26	36.78952983834619	39.0	35.0	40.0	31.0	41.0
27	36.72543205225663	39.0	35.0	40.0	31.0	41.0
28	36.54923680298148	39.0	35.0	40.0	30.0	41.0
29	36.60478570611701	39.0	35.0	40.0	30.0	41.0
30	36.29320219843795	39.0	35.0	40.0	30.0	41.0
31	36.359140537231504	39.0	35.0	40.0	30.0	41.0
32	35.984982273916756	39.0	35.0	40.0	28.0	41.0
33	35.918712958540475	39.0	35.0	41.0	27.0	41.0
34	35.60177866277388	39.0	35.0	41.0	25.0	41.0
35	35.490671438469974	39.0	35.0	41.0	25.0	41.0
36	35.40823136070393	39.0	35.0	40.0	25.0	41.0
37	35.297854706661916	39.0	35.0	41.0	23.0	41.0
38	35.115579444470605	39.0	35.0	40.0	23.0	41.0
39	35.06874222171395	39.0	35.0	40.0	23.0	41.0
40	34.94381605236426	38.0	34.0	40.0	23.0	41.0
41	34.77188986283308	38.0	34.0	40.0	22.0	41.0
42	34.828317333889444	38.0	35.0	40.0	22.0	41.0
43	34.86623298867818	38.0	35.0	40.0	22.0	41.0
44	35.16270593537884	39.0	35.0	40.0	23.0	41.0
45	35.24873966539075	39.0	35.0	40.0	23.0	41.0
46	35.11204230042179	39.0	35.0	40.0	23.0	41.0
47	34.751162117981046	38.0	34.0	40.0	22.0	41.0
48	34.819325803391834	38.0	34.0	40.0	22.0	41.0
49	34.91586467632239	38.0	35.0	40.0	23.0	41.0
50	34.7896024917424	38.0	34.0	40.0	22.0	41.0
51	33.966792016199015	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	29.0
10	33.0
11	23.0
12	15.0
13	27.0
14	35.0
15	56.0
16	81.0
17	171.0
18	416.0
19	1184.0
20	2964.0
21	5482.0
22	7157.0
23	8247.0
24	9317.0
25	10562.0
26	11440.0
27	11240.0
28	11101.0
29	11870.0
30	14324.0
31	18321.0
32	24324.0
33	33074.0
34	48850.0
35	53356.0
36	63460.0
37	92151.0
38	151592.0
39	152303.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.0261686769682	15.413552549259677	17.39618300583245	17.16409576793967
2	27.5264882173682	38.268831020309314	20.255632319997847	13.94904844232464
3	25.242211623197957	37.14189611909775	24.80158222951746	12.814310028186826
4	23.28904615508809	19.57793758535092	41.130433027695744	16.00258323186524
5	34.02587268165031	23.138626716268305	24.356109276089633	18.479391325991752
6	21.797364296237497	39.28651674055338	25.944662329886782	12.971456633322346
7	64.4007776604261	4.714532697391878	26.29985671135747	4.584832930824549
8	62.76405809580831	16.780916374595527	14.302359217226929	6.152666312369241
9	57.8372160294919	6.478395705377024	14.49717795373055	21.187210311400527
10	39.78970878097019	23.53404955230708	21.79574977632172	14.880491890401007
11	31.358551237462244	20.79420925523542	29.624018674613694	18.223220832688646
12	23.19190587348891	18.396512636981925	32.618818575051634	25.792762914477535
13	26.296224041546978	19.44487423562573	37.923189215006964	16.33571250782033
14	19.100577863586523	30.417151583238596	29.234784831585394	21.247485721589495
15	15.34264821629185	23.66052027904286	38.68053359883216	22.316297905833128
16	18.798124466031176	25.22256829755602	27.420602619558565	28.558704616854243
17	19.096407020470767	29.643123826950372	31.15404538146397	20.106423771114894
18	18.501994604812616	25.084661388083497	30.323240341470964	26.090103665632924
19	18.257798467551513	26.858346058889616	27.869304612817942	27.014550860740933
20	23.98571149874538	26.578899570134073	33.950259332261474	15.485129598859073
21	21.070695790812035	31.88717196655253	30.435987648922648	16.60614459371279
22	18.953522007924605	20.74200644462533	36.542101970387016	23.76236957706305
23	23.291737021614388	26.43466912432476	32.294972788612256	17.978621065448603
24	22.30203631324377	27.104560346045435	27.81642908557628	22.776974255134512
25	18.028402096185022	36.13389751834835	25.89447766917142	19.943222716295214
26	18.574244371043587	24.477198269772824	33.31911658851942	23.62944077066417
27	25.979509051402278	25.16942368366173	27.925005549912214	20.92606171502378
28	17.354070944695966	29.213392442701362	31.500898076703148	21.931638535899523
29	20.442782086901534	25.2719456983135	32.02238800949876	22.262884205286205
30	19.412718380636523	29.5588997046774	33.591432280980285	17.436949633705794
31	25.721993124836022	21.972001533793918	25.84671478832971	26.459290553040343
32	25.767334225804067	25.66642673106807	27.625781192188413	20.94045785093945
33	25.66844488096279	20.686305507531063	29.598320899287593	24.046928712218552
34	25.754956239783116	24.14460716712299	27.658206133830248	22.442230459263644
35	23.50364276055997	21.874188535563167	32.90795218330182	21.71421652057504
36	27.10752029922436	28.84797276843075	26.285460575441803	17.75904635690308
37	20.018836065684052	22.104526710213857	33.99196776341902	23.884669460683078
38	23.930279648303745	22.01734263476196	28.13852580877357	25.913851908160723
39	25.816038909929972	23.434487490834236	26.557238094597412	24.192235504638383
40	20.259803163113602	21.30574298188374	35.96262386394979	22.471829991052868
41	25.31311595616578	24.379519814868384	26.544591021923836	23.762773207042
42	21.22945691586333	22.155384087560797	35.5854989202898	21.029660076286067
43	26.78865261585862	23.81282332443105	26.46265413619821	22.93586992351212
44	23.331965476182468	21.396828813798763	30.923572663486958	24.347633046531808
45	21.512266987776737	20.27702470888188	28.925604267714313	29.285104035627075
46	27.90764946081762	24.87154475920108	26.48068294192437	20.740122838056926
47	17.949559706964635	24.07410646413411	37.86789190789164	20.10844192100961
48	22.92429919744906	24.718703540507633	27.91774021029122	24.43925705175209
49	20.9583521133393	20.19966229625095	36.74902960625895	22.092955984150798
50	22.863485613954833	21.289194152747037	31.66356095821757	24.183759275080558
51	20.61715023780533	21.117785955022168	29.329099703331966	28.93596410384054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	445.5
2	757.0
3	5423.5
4	10090.0
5	6898.0
6	3706.0
7	3623.0
8	3540.0
9	3512.5
10	3485.0
11	3349.5
12	3214.0
13	3105.5
14	2997.0
15	2841.5
16	2686.0
17	2552.0
18	2418.0
19	2387.0
20	2356.0
21	2422.5
22	2489.0
23	2735.0
24	2981.0
25	3650.5
26	4996.0
27	5672.0
28	6931.0
29	8190.0
30	9733.0
31	11276.0
32	12906.5
33	14537.0
34	16778.5
35	19020.0
36	20626.0
37	22232.0
38	23731.5
39	25231.0
40	27141.0
41	29051.0
42	30840.5
43	32630.0
44	36635.0
45	40640.0
46	90620.0
47	140600.0
48	103264.5
49	65929.0
50	64215.0
51	62501.0
52	54172.0
53	45843.0
54	42607.0
55	39371.0
56	36985.5
57	34600.0
58	31621.0
59	28642.0
60	25212.0
61	21782.0
62	18748.0
63	15714.0
64	13832.0
65	11950.0
66	9706.0
67	7462.0
68	6456.0
69	5450.0
70	4525.0
71	3600.0
72	3108.5
73	2617.0
74	2064.0
75	1215.0
76	919.0
77	802.0
78	685.0
79	470.5
80	256.0
81	174.0
82	92.0
83	69.0
84	46.0
85	33.5
86	21.0
87	14.5
88	8.0
89	4.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	743255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.37594539158392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5651177612876	25.288704708874626
2	8.48661072156088	5.834705334480147
3	3.6210458305216875	3.7343062119130845
4	2.3632895454195193	3.249612494313703
5	1.8113031171314082	3.1132628521057515
6	1.4788697016067538	3.0502526462221065
7	1.2638470757254123	3.041215664091486
8	1.0738743585917274	2.953235704669714
9	0.921873161592527	2.852123531478437
>10	5.323057124264392	27.73691947350781
>50	0.051893999731648606	1.254180747505576
>100	0.030502579773931445	2.085807427198988
>500	0.003169099197291578	0.7540063801267085
>1k	0.003169099197291578	3.1182936786204625
>5k	0.0011884121989843416	2.776219626448114
>10k+	0.0011884121989843416	9.157153518443293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	26698	3.5920377259486984	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC	20428	2.7484510699558027	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG	20119	2.7068771821245736	No Hit
GAACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT	7956	1.070426704159407	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	6611	0.889465930266194	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5820	0.7830421591513007	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	4715	0.6343717835736052	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCTGTGT	4270	0.5745000033635831	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC	3791	0.5100537500588628	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA	3717	0.5000975439115781	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT	2865	0.38546662989149083	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1193	0.16051018829338518	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG	1190	0.1601065583144412	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	1158	0.1558011718723722	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGT	888	0.11947447376741496	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTAT	869	0.11691815056743649	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATGCTGTG	762	0.10252201465176825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCC	757	0.10184929802019495	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16468103140913953	0.0
2	0.0	0.0	0.0	1.3492004762833751	0.0
3	0.0	0.0	0.0	1.6149235457548217	0.0
4	0.0	0.0	0.0	2.9960107903747706	0.0
5	0.0	0.0	0.0	7.39826842739033	0.0
6	0.0	0.0	0.0	8.425103093823788	0.0
7	0.0	0.0	0.0	9.60921890871908	0.0
8	0.0	0.0	0.0	10.787280273930213	0.0
9	0.0	0.0	0.0	11.123100416411594	0.0
10	0.0	0.0	0.0	14.50350149006734	0.0
11	0.0	0.0014799765894612212	0.0	15.332153836839309	0.0
12	1.3454332631465646E-4	0.0014799765894612212	0.0	18.92513336607221	0.0
13	1.3454332631465646E-4	0.0014799765894612212	0.0	19.379217092384174	0.0
14	1.3454332631465646E-4	0.0014799765894612212	0.0	19.660547187708122	0.0
15	1.3454332631465646E-4	0.0014799765894612212	0.0	20.397171899280867	0.0
16	1.3454332631465646E-4	0.0014799765894612212	0.0	20.88072061405574	0.0
17	1.3454332631465646E-4	0.0014799765894612212	0.0	21.383374481167298	0.0
18	1.3454332631465646E-4	0.0014799765894612212	0.0	21.825752938089888	0.0
19	1.3454332631465646E-4	0.0014799765894612212	0.0	22.82756254582882	0.0
20	1.3454332631465646E-4	0.0014799765894612212	0.0	23.27047917605667	0.0
21	1.3454332631465646E-4	0.0014799765894612212	0.0	23.673974611674325	0.0
22	1.3454332631465646E-4	0.0014799765894612212	0.0	24.192908221269956	0.0
23	1.3454332631465646E-4	0.0014799765894612212	0.0	24.594923680298148	0.0
24	1.3454332631465646E-4	0.0014799765894612212	0.0	24.928591129558495	0.0
25	1.3454332631465646E-4	0.0014799765894612212	0.0	25.234273566945397	0.0
26	1.3454332631465646E-4	0.0014799765894612212	0.0	25.533901554648136	0.0
27	1.3454332631465646E-4	0.0014799765894612212	0.0	25.921117247781716	0.0
28	1.3454332631465646E-4	0.0014799765894612212	0.0	26.212941722558206	0.0
29	1.3454332631465646E-4	0.0014799765894612212	0.0	26.529253082723965	0.0
30	1.3454332631465646E-4	0.0014799765894612212	0.0	26.89870905678401	0.0
31	1.3454332631465646E-4	0.0014799765894612212	0.0	27.230223812823326	0.0
32	1.3454332631465646E-4	0.0014799765894612212	0.0	27.557971355725826	0.0
33	1.3454332631465646E-4	0.0014799765894612212	0.0	27.879933535596802	0.0
34	1.3454332631465646E-4	0.0014799765894612212	0.0	28.21360098485715	0.0
35	2.6908665262931293E-4	0.0014799765894612212	0.0	28.560453680096334	0.0
36	2.6908665262931293E-4	0.0014799765894612212	0.0	28.8759577803042	0.0
37	2.6908665262931293E-4	0.0014799765894612212	0.0	29.213257899375048	0.0
38	2.6908665262931293E-4	0.0014799765894612212	0.0	29.579619376929855	0.0
39	2.6908665262931293E-4	0.0014799765894612212	0.0	30.09290216682027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.1644628E-6	45.000004	1
GCGACGA	20	7.031675E-4	45.000004	33
TACGCTT	20	7.031675E-4	45.000004	29
TTACGCG	20	7.031675E-4	45.000004	1
GACGATT	20	7.031675E-4	45.000004	27
CCGCAAT	20	7.031675E-4	45.000004	42
CGCGCAT	90	0.0	45.0	41
TCGATAG	25	3.889471E-5	45.0	1
CCGCGTA	105	0.0	45.0	40
CGGTCGA	165	0.0	45.0	41
CCCGGTC	105	0.0	45.0	39
GCGAACC	135	0.0	43.333332	33
CCGATGA	3115	0.0	43.12199	18
GATACCT	2380	0.0	43.014706	5
CCCGTCG	225	0.0	43.0	40
CGATGAA	3185	0.0	42.88069	19
ACGGCTG	2135	0.0	42.786884	8
GATGAAT	3405	0.0	42.753304	20
CGTTTTT	3390	0.0	42.67699	1
ACGGCTA	95	0.0	42.63158	30
>>END_MODULE