##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551751_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	226016
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07028263485771	31.0	30.0	33.0	28.0	34.0
2	30.976315836047007	31.0	31.0	34.0	28.0	34.0
3	30.895149015998868	31.0	30.0	34.0	27.0	34.0
4	34.79761167350984	35.0	35.0	37.0	32.0	37.0
5	34.48678854594365	35.0	35.0	37.0	32.0	37.0
6	34.67596541837746	35.0	35.0	37.0	32.0	37.0
7	35.191344860540845	36.0	35.0	37.0	32.0	37.0
8	34.50183615319269	36.0	35.0	37.0	30.0	37.0
9	36.21996672801926	39.0	35.0	39.0	32.0	39.0
10	35.98266936854028	37.0	35.0	39.0	32.0	39.0
11	36.3656511043466	37.0	35.0	39.0	32.0	39.0
12	36.442663351267164	38.0	35.0	39.0	32.0	39.0
13	36.35495717117372	39.0	35.0	39.0	32.0	39.0
14	37.333410024069096	39.0	36.0	40.0	32.0	41.0
15	37.39521538298174	39.0	36.0	41.0	32.0	41.0
16	37.6263140662608	39.0	36.0	41.0	33.0	41.0
17	37.269007503893526	39.0	36.0	41.0	32.0	41.0
18	37.34102895370239	39.0	36.0	40.0	32.0	41.0
19	37.26130008494974	39.0	36.0	40.0	32.0	41.0
20	36.927465312190286	39.0	35.0	40.0	31.0	41.0
21	37.034643565057344	39.0	35.0	40.0	31.0	41.0
22	37.03364806031431	39.0	35.0	40.0	31.0	41.0
23	36.99628787342489	39.0	35.0	41.0	31.0	41.0
24	36.98618681863231	39.0	35.0	41.0	31.0	41.0
25	36.831861461135496	39.0	35.0	40.0	31.0	41.0
26	36.79040864363585	39.0	35.0	40.0	31.0	41.0
27	36.63555677474161	39.0	35.0	40.0	30.0	41.0
28	36.425589338807875	39.0	35.0	40.0	30.0	41.0
29	36.5155077516636	39.0	35.0	41.0	30.0	41.0
30	36.24971683420643	38.0	35.0	40.0	30.0	41.0
31	36.324574366416535	39.0	35.0	40.0	30.0	41.0
32	35.93094736655812	39.0	35.0	40.0	28.0	41.0
33	35.79956286280617	39.0	35.0	40.0	27.0	41.0
34	35.662510618717256	39.0	35.0	40.0	26.0	41.0
35	35.54407652555571	39.0	35.0	41.0	25.0	41.0
36	35.5116894379159	39.0	35.0	40.0	25.0	41.0
37	35.341767839445	39.0	35.0	41.0	24.0	41.0
38	35.08017131530511	38.0	35.0	40.0	23.0	41.0
39	35.13956091604134	38.0	35.0	40.0	24.0	41.0
40	35.06103992637689	38.0	34.0	40.0	24.0	41.0
41	34.87813694605692	38.0	34.0	40.0	23.0	41.0
42	34.96244956109302	38.0	35.0	40.0	23.0	41.0
43	35.034948853178534	38.0	35.0	40.0	23.0	41.0
44	35.23590807730426	38.0	35.0	40.0	24.0	41.0
45	35.2599240761716	38.0	35.0	40.0	24.0	41.0
46	35.1150759238284	38.0	35.0	40.0	23.0	41.0
47	34.955892503185616	38.0	34.0	40.0	23.0	41.0
48	34.979523573552314	38.0	34.0	40.0	24.0	41.0
49	35.049496495823306	38.0	35.0	40.0	24.0	41.0
50	34.90600665439615	38.0	34.0	40.0	24.0	41.0
51	34.170921527679454	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	13.0
11	5.0
12	13.0
13	8.0
14	19.0
15	23.0
16	39.0
17	58.0
18	113.0
19	237.0
20	629.0
21	1366.0
22	1997.0
23	2477.0
24	2853.0
25	3227.0
26	3571.0
27	3494.0
28	3287.0
29	3573.0
30	4333.0
31	5464.0
32	7302.0
33	10145.0
34	16271.0
35	17474.0
36	19116.0
37	27639.0
38	45297.0
39	45955.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.596789607815374	15.359532068526121	17.74077941384681	15.302898909811693
2	27.984301996318845	34.7723170041059	20.32909174571712	16.914289253858133
3	27.790952852895366	33.436128415687385	24.04918235877106	14.723736372646185
4	25.14379512954835	20.71003822738213	37.13099957525131	17.015167067818208
5	32.98306314597197	25.107956958799377	23.32135777998018	18.58762211524848
6	23.004566048421353	39.33482585303695	24.300934447118788	13.359673651422908
7	65.69446410873566	5.871708197649724	23.243044740195383	5.190782953419227
8	64.27553801500778	16.966940393600453	12.436730142998725	6.3207914483930345
9	59.36438128274104	7.656537590259097	13.17296120628628	19.806119920713577
10	43.728762565482086	20.216267874840717	20.94055288121195	15.114416678465242
11	35.4085551465383	21.454233328613903	24.744265892680165	18.392945632167635
12	26.750318561517766	19.71807305677474	27.659103780263344	25.872504601444145
13	28.015715701543254	21.35910731983576	33.16535112558403	17.459825853036953
14	20.475984001132662	31.915882061446975	27.07640167067818	20.53173226674218
15	17.1686960215206	22.820065836047004	37.04737717683704	22.963860965595355
16	18.91724479682854	24.086347869177406	28.942641228939543	28.05376610505451
17	19.81718108452499	28.74309783378168	30.05008494973807	21.389636131955257
18	20.35032918023503	24.266866062579638	29.27978550191137	26.103019255273963
19	20.629955401387512	26.134432960498373	26.92641228939544	26.309199348718675
20	27.613974231912785	24.963276936146116	31.68404006795979	15.73870876398131
21	23.474001840577657	32.12117726178678	26.68970338383123	17.715117513804334
22	20.815782953419227	22.211701826419368	34.07103921846241	22.901476001698995
23	24.191650148662042	27.218869460569163	29.397918731417246	19.19156165935155
24	22.94572065694464	26.32601231771202	27.223736372646183	23.504530652697152
25	19.280935862947757	33.303394449950446	26.02957312756619	21.38609655953561
26	18.928305960639953	24.716834206427862	32.52734319694181	23.827516635990374
27	24.996902874132804	27.60954976638822	26.410077162678746	20.983470196800226
28	18.37436287696446	28.83910873566473	31.433615319269432	21.352913068101373
29	21.504229789041485	22.6452994478267	31.41326277785643	24.43720798527538
30	19.634008211808013	33.2113655670395	28.545766671386097	18.608859549766386
31	24.76107886167351	24.40490938694606	23.44480036811553	27.3892113832649
32	28.180748265609513	27.28036953136061	23.177562650431828	21.361319552598047
33	24.800456604842136	28.169687101798104	23.547890414837887	23.481965878521873
34	22.801925527396293	26.98393034121478	24.900891972249752	25.313252159139175
35	24.981417244796827	27.03923616027184	24.53852824578791	23.440818349143424
36	22.424076171598468	32.043306668554436	23.433739204304118	22.09887795554297
37	20.344134928500637	29.572242673085093	26.834825853036953	23.248796545377317
38	23.06341143989806	28.62717683703809	22.791306810137336	25.51810491292652
39	22.169669403936005	29.11121336542546	24.7947047996602	23.924412430978336
40	22.38691066119213	27.8272334701968	26.409192269573833	23.376663599037236
41	19.51631742885459	31.477859974515077	24.11422200198216	24.891600594648168
42	24.492513804332436	24.35447047996602	28.48692127990939	22.666094435792157
43	22.686889423757613	28.730709330312898	24.18412855727028	24.39827268865921
44	22.147104629760726	25.197773608948037	28.653281183633016	24.001840577658218
45	21.35601019396857	23.04969559677191	27.40248477983859	28.19180942942093
46	25.98621336542546	27.580348293926093	24.719931332295058	21.713507008353393
47	18.455773042616453	28.969188022086932	32.344170324224834	20.230868611071784
48	21.962161970833925	27.034811694747273	25.72826702534334	25.274759309075463
49	21.915705082825994	22.9908502052952	32.6910484213507	22.402396290528102
50	22.05153617443013	25.190694464108738	27.823693897777147	24.934075463683985
51	20.317145688800792	23.727523715135213	26.2286740761716	29.726656519892398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	191.0
2	312.0
3	1212.5
4	2113.0
5	1487.0
6	861.0
7	874.0
8	887.0
9	869.0
10	851.0
11	835.5
12	820.0
13	804.0
14	788.0
15	752.0
16	716.0
17	694.0
18	672.0
19	689.5
20	707.0
21	727.5
22	748.0
23	772.0
24	796.0
25	993.5
26	1379.5
27	1568.0
28	2055.0
29	2542.0
30	2906.0
31	3270.0
32	3706.0
33	4142.0
34	4424.0
35	4706.0
36	5484.5
37	6263.0
38	6773.0
39	7283.0
40	8266.0
41	9249.0
42	9701.5
43	10154.0
44	11667.5
45	13181.0
46	23731.0
47	34281.0
48	27055.5
49	19830.0
50	20195.0
51	20560.0
52	18228.0
53	15896.0
54	14353.0
55	12810.0
56	12288.5
57	11767.0
58	10900.5
59	10034.0
60	9122.0
61	8210.0
62	7177.5
63	6145.0
64	5305.0
65	4465.0
66	3652.5
67	2840.0
68	2392.5
69	1945.0
70	1597.5
71	1250.0
72	998.5
73	747.0
74	693.5
75	465.0
76	290.0
77	276.0
78	262.0
79	182.0
80	102.0
81	61.5
82	21.0
83	17.0
84	13.0
85	12.5
86	12.0
87	7.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	226016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.44541979328897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51328182876495	25.25573410731984
2	7.623822626732987	5.099638963613195
3	2.9421102762197058	2.952003397989523
4	1.8150068790348184	2.4281466798810705
5	1.329505767806117	2.223293926093728
6	1.1654672452111334	2.338772476284865
7	0.9683564398349032	2.2670961347869176
8	0.829452852153667	2.21931190712162
9	0.8069636998624193	2.4290315729859833
>10	6.921367340459309	40.15335197508141
>50	0.04497830458249551	0.952144980886309
>100	0.026457826224997354	1.645458728585587
>500	0.005291565244999471	1.5556420784369247
>1k	0.005291565244999471	3.7709719665864365
>5k	0.0026457826224997355	4.709401104346595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	5497	2.4321286988531785	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	5147	2.277272405493416	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	4643	2.054279343055359	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1712	0.7574684978054651	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAAGAT	1167	0.5163351267166927	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1001	0.44288899900891976	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	971	0.4296156024352258	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTA	885	0.39156519892396996	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	833	0.3685579781962339	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	827	0.36590329888149514	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	367	0.162377884751522	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	343	0.1517591674925669	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATG	257	0.11370876398131106	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT	237	0.10485983293218179	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4244655245646327E-4	0.0	0.0	0.15751097267450093	0.0
2	4.4244655245646327E-4	0.0	0.0	1.2738036245221578	0.0
3	4.4244655245646327E-4	0.0	0.0	1.5091851904289961	0.0
4	4.4244655245646327E-4	0.0	0.0	2.1657758742743876	0.0
5	4.4244655245646327E-4	0.0	0.0	5.01026476001699	0.0
6	4.4244655245646327E-4	0.0	0.0	5.5730567747416115	0.0
7	4.4244655245646327E-4	0.0	0.0	6.4473311623955825	0.0
8	4.4244655245646327E-4	0.0	0.0	7.357886167350984	0.0
9	4.4244655245646327E-4	0.0	0.0	7.58353390910378	0.0
10	4.4244655245646327E-4	0.0	0.0	10.43333215347586	0.0
11	4.4244655245646327E-4	0.0	0.0	11.219559677190995	0.0
12	4.4244655245646327E-4	0.0	0.0	14.40915687384964	0.0
13	4.4244655245646327E-4	0.0	0.0	14.793642927934306	0.0
14	4.4244655245646327E-4	0.0	0.0	14.980797819623389	0.0
15	4.4244655245646327E-4	0.0	0.0	15.634291377601587	0.0
16	4.4244655245646327E-4	0.0	0.0	16.087356647317005	0.0
17	4.4244655245646327E-4	0.0	0.0	16.565198923969984	0.0
18	4.4244655245646327E-4	0.0	0.0	17.02357355231488	0.0
19	4.4244655245646327E-4	0.0	0.0	17.971736514229082	0.0
20	4.4244655245646327E-4	0.0	0.0	18.414625513238	0.0
21	4.4244655245646327E-4	0.0	0.0	18.79999646042758	0.0
22	4.4244655245646327E-4	0.0	0.0	19.219435792156307	0.0
23	4.4244655245646327E-4	0.0	0.0	19.60082472037378	0.0
24	4.4244655245646327E-4	0.0	0.0	19.933986974373497	0.0
25	4.4244655245646327E-4	0.0	0.0	20.199454905847375	0.0
26	4.4244655245646327E-4	0.0	0.0	20.469789749398274	0.0
27	4.4244655245646327E-4	0.0	0.0	20.817110293076595	0.0
28	4.4244655245646327E-4	0.0	0.0	21.1047005521733	0.0
29	4.4244655245646327E-4	0.0	0.0	21.365744018122612	0.0
30	4.4244655245646327E-4	0.0	0.0	21.660413422058614	0.0
31	4.4244655245646327E-4	0.0	0.0	21.95862239841427	0.0
32	4.4244655245646327E-4	0.0	0.0	22.252406909245362	0.0
33	4.4244655245646327E-4	0.0	0.0	22.5355727028175	0.0
34	4.4244655245646327E-4	0.0	0.0	22.813871584312615	0.0
35	4.4244655245646327E-4	0.0	0.0	23.12756619000425	0.0
36	4.4244655245646327E-4	0.0	0.0	23.419580914625513	0.0
37	4.4244655245646327E-4	0.0	0.0	23.729735947897495	0.0
38	8.848931049129265E-4	0.0	0.0	24.05670395016282	0.0
39	8.848931049129265E-4	0.0	0.0	24.396502902449384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	35	1.2061355E-7	45.000004	40
ACGGGAT	35	1.2061355E-7	45.000004	5
TCGTTGG	20	7.021251E-4	45.0	12
CATATTC	20	7.021251E-4	45.0	33
CGTATGG	20	7.021251E-4	45.0	2
CAACGAG	45	3.8198777E-10	45.0	14
CGTGCGG	20	7.021251E-4	45.0	2
TAGACGG	20	7.021251E-4	45.0	2
CGTTGTG	20	7.021251E-4	45.0	34
ATCGTTG	20	7.021251E-4	45.0	11
ACGGCTA	20	7.021251E-4	45.0	30
CTAGTAG	20	7.021251E-4	45.0	1
CGCTAGG	25	3.880832E-5	45.0	2
GAGCGAT	20	7.021251E-4	45.0	25
TCTCGTA	20	7.021251E-4	45.0	28
CGCAGCG	20	7.021251E-4	45.0	13
TTTGCGG	25	3.880832E-5	45.0	2
CACGTGA	25	3.880832E-5	45.0	43
CACCGTG	20	7.021251E-4	45.0	16
ATACGCC	25	3.880832E-5	45.0	28
>>END_MODULE