FastQCFastQC Report
Sat 18 Jun 2016
SRR3551747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698232
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC178192.5520170946046585No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG166722.387745047491378No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC165832.374998567811272No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC58830.8425566287423091TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT36620.5244675122308917No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT33870.48508232220809133No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT32620.46717996310681836No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.42192279929880044No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA27370.3919900548814721No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC25420.3640623746834863No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT14950.21411221485122425No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC13260.18990822534630322TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT12890.18460912705232643TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG9440.13519861593281315No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCATGCAC8080.11572084923062821No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC7090.10154218082242006TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG207.031381E-445.00000439
TAACGAA207.031381E-445.00000445
CATAGCG207.031381E-445.0000041
TTACGCG207.031381E-445.0000041
TACGTAA207.031381E-445.00000414
TACCGCT207.031381E-445.00000429
CGCTCGA302.1642736E-644.99999641
ACATGCG302.1642736E-644.9999961
CTTAGCG253.8892278E-544.9999961
CTCGAAT2300.044.02173643
ACGGCCA950.042.6315830
CCGATGA18000.042.62518
CGTTTTT20150.042.5434271
TAAGCCG850.042.35294345
ACTGCGA800.042.18750430
TGATACC19350.041.627914
CGATGAA18650.041.62198319
GATGAAT20250.041.55555720
GATACCT19400.041.404645
TACGGGT603.6379788E-1241.2499964