##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698232
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114222779821034	31.0	30.0	33.0	28.0	34.0
2	30.98900651932309	31.0	31.0	34.0	28.0	34.0
3	30.8666875766221	31.0	30.0	34.0	27.0	34.0
4	34.822623426024585	35.0	35.0	37.0	32.0	37.0
5	34.51519552240516	35.0	35.0	37.0	32.0	37.0
6	34.707766186597006	35.0	35.0	37.0	32.0	37.0
7	35.243832995336795	36.0	35.0	37.0	33.0	37.0
8	34.65392448355274	36.0	35.0	37.0	32.0	37.0
9	36.48789227649263	39.0	35.0	39.0	32.0	39.0
10	36.0996502595126	37.0	35.0	39.0	32.0	39.0
11	36.47175294171565	38.0	35.0	39.0	32.0	39.0
12	36.637438559103565	39.0	35.0	39.0	32.0	39.0
13	36.61339927130238	39.0	35.0	39.0	32.0	39.0
14	37.63610232702025	39.0	37.0	41.0	32.0	41.0
15	37.61705135255904	39.0	36.0	41.0	32.0	41.0
16	37.85391388535616	39.0	37.0	41.0	33.0	41.0
17	37.508318152132816	39.0	36.0	41.0	32.0	41.0
18	37.54263769062432	39.0	36.0	40.0	32.0	41.0
19	37.502507762462905	39.0	36.0	40.0	32.0	41.0
20	37.24382010563824	39.0	35.0	41.0	32.0	41.0
21	37.32045938885643	39.0	35.0	40.0	32.0	41.0
22	37.40899443165023	39.0	36.0	41.0	32.0	41.0
23	37.37658400073328	39.0	36.0	41.0	32.0	41.0
24	37.309729430905485	39.0	35.0	41.0	32.0	41.0
25	37.088712634196085	39.0	35.0	40.0	32.0	41.0
26	37.145172378235316	39.0	35.0	40.0	32.0	41.0
27	37.09742893479531	39.0	35.0	41.0	31.0	41.0
28	36.926280949598414	39.0	35.0	41.0	31.0	41.0
29	37.08186963645321	39.0	35.0	41.0	31.0	41.0
30	36.747542364142575	39.0	35.0	41.0	30.0	41.0
31	36.795295832903676	39.0	35.0	40.0	30.0	41.0
32	36.60950944671685	39.0	35.0	41.0	30.0	41.0
33	36.592550899987394	39.0	35.0	41.0	30.0	41.0
34	36.44188321360235	39.0	35.0	41.0	30.0	41.0
35	36.34206538800857	39.0	35.0	41.0	30.0	41.0
36	36.254087466630004	39.0	35.0	41.0	29.0	41.0
37	36.338462001168665	39.0	35.0	41.0	30.0	41.0
38	35.991373927290645	39.0	35.0	41.0	27.0	41.0
39	35.97742584126766	39.0	35.0	41.0	28.0	41.0
40	35.88910562678308	39.0	35.0	41.0	27.0	41.0
41	35.897814766438664	39.0	35.0	40.0	27.0	41.0
42	35.87406191638309	39.0	35.0	41.0	28.0	41.0
43	35.93748066545217	39.0	35.0	41.0	28.0	41.0
44	36.128004731951556	39.0	35.0	41.0	29.0	41.0
45	36.160208927691656	39.0	35.0	41.0	29.0	41.0
46	36.02812818661992	39.0	35.0	40.0	29.0	41.0
47	35.77985397403728	39.0	35.0	40.0	28.0	41.0
48	35.8380151582855	39.0	35.0	40.0	28.0	41.0
49	35.956611842482154	39.0	35.0	40.0	29.0	41.0
50	35.80358677345066	39.0	35.0	40.0	28.0	41.0
51	34.989620928287444	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	34.0
10	41.0
11	24.0
12	36.0
13	18.0
14	37.0
15	61.0
16	66.0
17	117.0
18	258.0
19	537.0
20	1305.0
21	2981.0
22	4809.0
23	5960.0
24	6527.0
25	7240.0
26	7768.0
27	8155.0
28	8592.0
29	9632.0
30	11891.0
31	15684.0
32	20978.0
33	28818.0
34	44593.0
35	50532.0
36	59473.0
37	90565.0
38	153912.0
39	157551.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.74010357588882	17.728061733062937	19.667102052040008	13.864732639008237
2	27.697527469379807	35.37334295764158	21.304236987133216	15.624892585845393
3	27.57178129905246	34.184339875571446	24.40807639867551	13.83580242670058
4	25.549101158354244	21.26800261231224	36.63395547611682	16.548940753216694
5	32.806574319137475	26.351413283836887	22.935213510695583	17.906798886330044
6	22.83954330365838	39.91925319950962	23.810710480184234	13.430493016647763
7	68.11217474993984	5.748948773473573	21.440867791794133	4.698008684792447
8	67.38834656675718	15.140956014619784	11.618774275598941	5.851923143024095
9	62.2581033238236	7.664357978436967	11.963931759071484	18.11360693866795
10	40.23777197263947	24.745070406397872	20.109505150150667	14.907652470811994
11	30.839749538835232	22.26065261975962	27.849912350049838	19.04968549135531
12	24.42841347861456	19.500108846343338	30.354094341135895	25.717383333906206
13	25.26624388455413	21.536394779958524	35.49994844120579	17.697412894281555
14	19.954112673151617	30.16533186677208	27.83544724389601	22.045108216180296
15	17.134276286391916	24.67918972490519	36.481427376573976	21.70510661212892
16	19.242601313030626	25.84341594197917	27.675328544094224	27.23865420089598
17	19.28642628811054	29.363162960162235	30.491870896779293	20.858539854947924
18	18.616591619977314	25.86676061824723	30.204717056794877	25.31193070498058
19	19.753463032344552	27.050178164277778	27.768277592548035	25.42808121082964
20	23.804695287526208	26.791381661109774	32.50982481467478	16.89409823668924
21	21.90017071689639	31.645355698392514	28.95298410843387	17.501489476277225
22	19.367201732375484	22.75275266673541	33.315860630850494	24.56418497003861
23	21.97836822145075	27.92123534870931	30.381449145842645	19.718947283997295
24	21.634786145579117	26.37275289588561	28.11501048362149	23.877450474913783
25	18.19824929249877	34.749051891061995	26.032321635215805	21.02037718122343
26	18.27587392156189	25.203657237136078	33.007367178817354	23.513101662484676
27	24.201698002956036	27.14642124680622	26.388506971894728	22.263373778343016
28	17.220637266696457	29.107087615577633	32.203336426860986	21.468938690864928
29	20.445496625763358	22.83854077154871	33.80065651531296	22.91530608737497
30	19.490369962992244	29.11682649892872	28.91990054881472	22.472902989264313
31	21.102011938725237	26.27865809644932	25.685159087523918	26.93417087730153
32	23.919986480138405	28.50313362893709	28.042398515106726	19.53448137581778
33	23.939464246840593	24.39060369619267	24.30467237250656	27.36525968446018
34	20.590863781665693	24.79534023075425	28.92720471132804	25.68659127625202
35	20.766593338603787	26.077722017896633	26.205330033570505	26.95035460992908
36	19.004571546420102	29.680822420055225	27.05375863609803	24.260847397426645
37	18.032401837784576	25.432377777013944	31.45859828824803	25.07662209695345
38	21.022811902061207	28.73443210852553	24.635221530952464	25.607534458460794
39	21.804357290986374	23.999043297929628	29.333516653490534	24.863082757593467
40	22.29903527767275	25.661384754637428	28.16270236826728	23.876877599422542
41	17.91553523757147	26.06010609654098	25.137346899024966	30.88701176686259
42	18.954301722063725	26.992174520789654	30.81354048511097	23.239983272035655
43	22.201503225289017	23.381053861753685	26.798399385877474	27.619043527079825
44	21.17935013004274	23.03374809518899	30.182088474890868	25.604813299877407
45	19.989487734735732	23.01799401917987	27.92452938278395	29.06798886330045
46	25.07791106680874	25.887384135931896	27.071231338580876	21.96347345867849
47	18.207844956977052	24.799350359192935	34.843289909371094	22.14951477445892
48	20.45108216180295	25.806322253921333	27.048316318931242	26.694279265344473
49	19.533622062580918	22.561698690406626	34.34503145086447	23.559647796147985
50	21.519351734094112	22.359473641998648	29.777781596947715	26.34339302695952
51	20.053936227500316	21.41737989665326	28.118161298823313	30.41052257702311
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	423.0
2	736.0
3	3225.0
4	5714.0
5	4097.0
6	2480.0
7	2326.0
8	2172.0
9	2168.5
10	2165.0
11	2075.0
12	1985.0
13	1960.0
14	1935.0
15	1882.0
16	1829.0
17	1785.0
18	1741.0
19	1846.0
20	1951.0
21	2045.5
22	2140.0
23	2557.5
24	2975.0
25	3700.5
26	5597.5
27	6769.0
28	7740.0
29	8711.0
30	10003.5
31	11296.0
32	13104.0
33	14912.0
34	16158.5
35	17405.0
36	19529.5
37	21654.0
38	23035.5
39	24417.0
40	27524.0
41	30631.0
42	33129.5
43	35628.0
44	39105.5
45	42583.0
46	80391.0
47	118199.0
48	93327.5
49	68456.0
50	66575.0
51	64694.0
52	54220.0
53	43746.0
54	39937.5
55	36129.0
56	33105.0
57	30081.0
58	26878.5
59	23676.0
60	21544.5
61	19413.0
62	17354.5
63	15296.0
64	13235.5
65	11175.0
66	9155.0
67	7135.0
68	5846.0
69	4557.0
70	3993.5
71	3430.0
72	2944.5
73	2459.0
74	1943.0
75	1157.0
76	887.0
77	750.5
78	614.0
79	459.5
80	305.0
81	207.5
82	110.0
83	73.0
84	36.0
85	35.5
86	35.0
87	20.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	698232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.68633578291084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74723706402607	23.68466052455569
2	9.332761899446224	5.9144205465561965
3	3.2762522691712204	3.1143728853144865
4	1.8211511883091933	2.308224322568488
5	1.2280939676247506	1.9456898915562544
6	0.8916305400868076	1.695150281249324
7	0.7908435307762078	1.7541253567542392
8	0.6683579726865951	1.6942252116586405
9	0.6003305712163483	1.712004845427709
>10	6.495938452400911	39.27545904599497
>50	0.08944550673312601	1.9213275607658657
>100	0.04837359037310207	2.6890198930009084
>500	0.0036508370092907224	0.7805619155957682
>1k	0.004107191635452062	3.274658232786544
>5k	4.563546261613403E-4	0.8506939143108381
>10k+	0.0013690638784840208	7.385405571904087
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	17819	2.5520170946046585	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	16672	2.387745047491378	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	16583	2.374998567811272	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	5883	0.8425566287423091	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT	3662	0.5244675122308917	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	3387	0.48508232220809133	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	3262	0.46717996310681836	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2946	0.42192279929880044	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA	2737	0.3919900548814721	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	2542	0.3640623746834863	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	1495	0.21411221485122425	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1326	0.18990822534630322	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1289	0.18460912705232643	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG	944	0.13519861593281315	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCATGCAC	808	0.11572084923062821	No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	709	0.10154218082242006	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4321887281018344E-4	0.0	0.0	0.19076753858316434	0.0
2	1.4321887281018344E-4	0.0	0.0	1.4496614305846767	0.0
3	1.4321887281018344E-4	0.0	0.0	1.7683234225873348	0.0
4	1.4321887281018344E-4	0.0	0.0	2.62334009326413	0.0
5	1.4321887281018344E-4	0.0	0.0	6.039826304151056	0.0
6	1.4321887281018344E-4	0.0	0.0	6.944683142565795	0.0
7	1.4321887281018344E-4	0.0	0.0	8.032430481559137	0.0
8	1.4321887281018344E-4	0.0	0.0	9.199091419470893	0.0
9	1.4321887281018344E-4	0.0	0.0	9.491974014367717	0.0
10	1.4321887281018344E-4	0.0	0.0	12.626462264691392	0.0
11	1.4321887281018344E-4	0.0	0.0	13.73268483827725	0.0
12	1.4321887281018344E-4	0.0	0.0	17.26231395868422	0.0
13	1.4321887281018344E-4	0.0	0.0	17.774321428980624	0.0
14	1.4321887281018344E-4	0.0	0.0	18.01149188235429	0.0
15	1.4321887281018344E-4	0.0	0.0	18.687198524272734	0.0
16	1.4321887281018344E-4	0.0	0.0	19.305331179321485	0.0
17	1.4321887281018344E-4	0.0	0.0	20.022857732100505	0.0
18	1.4321887281018344E-4	0.0	0.0	20.681950984772968	0.0
19	1.4321887281018344E-4	0.0	0.0	21.789605747086927	0.0
20	1.4321887281018344E-4	0.0	0.0	22.38396406924919	0.0
21	1.4321887281018344E-4	0.0	0.0	22.960133594564557	0.0
22	1.4321887281018344E-4	0.0	0.0	23.655890878676427	0.0
23	1.4321887281018344E-4	0.0	0.0	24.253829672658945	0.0
24	1.4321887281018344E-4	0.0	0.0	24.732467145590576	0.0
25	1.4321887281018344E-4	0.0	0.0	25.147372220121678	0.0
26	1.4321887281018344E-4	0.0	0.0	25.56872214392924	0.0
27	1.4321887281018344E-4	0.0	0.0	26.03747751463697	0.0
28	1.4321887281018344E-4	0.0	0.0	26.450807181567157	0.0
29	1.4321887281018344E-4	0.0	0.0	26.88375783407234	0.0
30	1.4321887281018344E-4	0.0	0.0	27.348932732959817	0.0
31	1.4321887281018344E-4	0.0	0.0	27.799069650202224	0.0
32	1.4321887281018344E-4	0.0	0.0	28.23359571030832	0.0
33	1.4321887281018344E-4	0.0	0.0	28.63761615050585	0.0
34	1.4321887281018344E-4	0.0	0.0	29.039774745356844	0.0
35	1.4321887281018344E-4	0.0	0.0	29.460695012545973	0.0
36	1.4321887281018344E-4	0.0	0.0	29.88104240424386	0.0
37	1.4321887281018344E-4	0.0	0.0	30.305256705507624	0.0
38	1.4321887281018344E-4	0.0	0.0	30.714289806253507	0.0
39	1.4321887281018344E-4	0.0	0.0	31.156120028872923	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	20	7.031381E-4	45.000004	39
TAACGAA	20	7.031381E-4	45.000004	45
CATAGCG	20	7.031381E-4	45.000004	1
TTACGCG	20	7.031381E-4	45.000004	1
TACGTAA	20	7.031381E-4	45.000004	14
TACCGCT	20	7.031381E-4	45.000004	29
CGCTCGA	30	2.1642736E-6	44.999996	41
ACATGCG	30	2.1642736E-6	44.999996	1
CTTAGCG	25	3.8892278E-5	44.999996	1
CTCGAAT	230	0.0	44.021736	43
ACGGCCA	95	0.0	42.63158	30
CCGATGA	1800	0.0	42.625	18
CGTTTTT	2015	0.0	42.543427	1
TAAGCCG	85	0.0	42.352943	45
ACTGCGA	80	0.0	42.187504	30
TGATACC	1935	0.0	41.62791	4
CGATGAA	1865	0.0	41.621983	19
GATGAAT	2025	0.0	41.555557	20
GATACCT	1940	0.0	41.40464	5
TACGGGT	60	3.6379788E-12	41.249996	4
>>END_MODULE