SRR3551745_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306807
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC	9529	3.105861339539189	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	8633	2.813821066664059	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	8629	2.812517315445867	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3457	1.1267669903229065	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2074	0.6759950066328343	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	1935	0.6306896518006434	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCTCGTTT	1864	0.6075480676777257	No Hit
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1622	0.5286711189770767	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTA	1573	0.5127001665542181	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC	1479	0.4820620129266933	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1090	0.35527220695746836	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	762	0.24836460706567973	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATG	505	0.16459859129680873	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	393	0.1280935571874175	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	388	0.1264638681646768	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACCTCGTT	384	0.12516011694648427	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	383	0.12483417914193613	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTAT	358	0.11668573402823273	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGT	339	0.11049291574181815	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	332	0.1082113511099812	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	30	2.160281E-6	45.000004	34
ATACTCC	30	2.160281E-6	45.000004	28
CCCCGTA	30	2.160281E-6	45.000004	40
CACAACG	95	0.0	45.000004	12
ACTGCGA	25	3.884099E-5	45.0	30
GTTTCGA	20	7.0251944E-4	45.0	44
ACGTTAT	20	7.0251944E-4	45.0	21
GCGCGAA	20	7.0251944E-4	45.0	13
TCGATCA	20	7.0251944E-4	45.0	17
TCCTACG	20	7.0251944E-4	45.0	44
ACGGGCC	20	7.0251944E-4	45.0	5
CGTTATT	25	3.884099E-5	45.0	1
AAACGCC	20	7.0251944E-4	45.0	5
CCCGTAA	20	7.0251944E-4	45.0	41
TCGTGAA	20	7.0251944E-4	45.0	43
GAAACGC	20	7.0251944E-4	45.0	4
GCGATTA	25	3.884099E-5	45.0	9
CCGACGG	20	7.0251944E-4	45.0	40
CGATCGA	55	1.8189894E-12	45.0	41
CGATCAC	50	2.1827873E-11	45.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content