##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306807
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04353225317545	31.0	30.0	33.0	28.0	34.0
2	30.934730954639235	31.0	30.0	34.0	28.0	34.0
3	30.816184115747035	31.0	30.0	34.0	27.0	34.0
4	34.82719429478467	35.0	35.0	37.0	32.0	37.0
5	34.511181296385026	35.0	35.0	37.0	32.0	37.0
6	34.68988321648463	35.0	35.0	37.0	32.0	37.0
7	35.12323056514356	35.0	35.0	37.0	32.0	37.0
8	34.37213948834283	35.0	35.0	37.0	30.0	37.0
9	35.970088687676615	39.0	35.0	39.0	31.0	39.0
10	35.92367188493092	37.0	35.0	39.0	32.0	39.0
11	36.38492602841526	37.0	35.0	39.0	32.0	39.0
12	36.402976464031134	38.0	35.0	39.0	32.0	39.0
13	36.28656451775872	38.0	35.0	39.0	32.0	39.0
14	37.267174477766154	39.0	36.0	40.0	32.0	41.0
15	37.21763519085288	39.0	36.0	41.0	31.0	41.0
16	37.49320908584224	39.0	36.0	40.0	33.0	41.0
17	37.08357371246419	39.0	35.0	40.0	31.0	41.0
18	37.14901876423941	39.0	36.0	40.0	32.0	41.0
19	37.07466909164393	39.0	35.0	40.0	32.0	41.0
20	36.70127474275359	39.0	35.0	40.0	30.0	41.0
21	36.885393749164784	39.0	35.0	40.0	31.0	41.0
22	36.91252155263732	39.0	35.0	40.0	31.0	41.0
23	36.853458363075156	39.0	35.0	40.0	31.0	41.0
24	36.82014426007229	39.0	35.0	40.0	31.0	41.0
25	36.7041853673482	38.0	35.0	40.0	31.0	41.0
26	36.515415228466104	38.0	35.0	40.0	30.0	41.0
27	36.42180589099988	38.0	35.0	40.0	30.0	41.0
28	36.21172267907838	38.0	35.0	40.0	30.0	41.0
29	36.12685173415209	38.0	35.0	40.0	30.0	41.0
30	35.882818840508854	38.0	35.0	40.0	29.0	41.0
31	36.07536333916762	38.0	35.0	40.0	30.0	41.0
32	35.593115541692335	38.0	35.0	40.0	26.0	41.0
33	35.53908157245434	38.0	35.0	40.0	26.0	41.0
34	35.10542132350305	38.0	34.0	40.0	24.0	41.0
35	34.991867851776526	38.0	34.0	40.0	23.0	41.0
36	35.06457805721512	38.0	34.0	40.0	25.0	41.0
37	35.200106255724286	38.0	35.0	40.0	25.0	41.0
38	35.267715534521706	38.0	35.0	40.0	25.0	41.0
39	34.863722144540375	38.0	34.0	40.0	23.0	41.0
40	35.0190771397002	38.0	34.0	40.0	25.0	41.0
41	34.750455498081855	38.0	34.0	40.0	23.0	41.0
42	34.59827187776028	38.0	34.0	40.0	23.0	41.0
43	34.617381611240944	38.0	34.0	40.0	22.0	41.0
44	34.889885172111455	38.0	35.0	40.0	23.0	41.0
45	34.95066931328164	38.0	35.0	40.0	23.0	41.0
46	34.79134113628437	38.0	34.0	40.0	23.0	41.0
47	34.53862200014993	37.0	34.0	40.0	23.0	41.0
48	34.58043656109541	38.0	34.0	40.0	23.0	41.0
49	34.675988487876744	38.0	34.0	40.0	23.0	41.0
50	34.50771331814463	37.0	34.0	40.0	23.0	41.0
51	33.68216500927293	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	8.0
10	17.0
11	9.0
12	16.0
13	9.0
14	22.0
15	24.0
16	36.0
17	81.0
18	138.0
19	280.0
20	644.0
21	1522.0
22	2864.0
23	3743.0
24	4551.0
25	4827.0
26	4726.0
27	4587.0
28	4707.0
29	5402.0
30	6624.0
31	8387.0
32	10899.0
33	14737.0
34	24236.0
35	24994.0
36	26543.0
37	38624.0
38	60914.0
39	52622.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.02100017274703	15.508772616009415	16.79427131714726	16.67595589409629
2	27.97621957778017	36.779799678625324	18.87375451016437	16.370226233430138
3	25.233778890312152	36.46755126186821	23.97305146231996	14.325618385499677
4	24.85438728581812	19.821907583594896	39.61513264038956	15.70857249019742
5	33.8551597584149	23.73511686499982	23.361918078792204	19.047805297793076
6	21.198668870006227	40.391190553018674	24.948257373527984	13.461883203447117
7	61.30661947087257	5.750194747838218	27.149315367641545	5.793870413647668
8	58.99343887199444	18.71860811519946	14.62450335227032	7.663449660535776
9	54.428028043688705	7.219848308545764	15.321358378394233	23.030765269371297
10	42.97750703210813	18.740445948104185	22.58064516129032	15.701401858497361
11	36.17388129997034	20.763215963129916	24.94727956011434	18.115623176785405
12	26.66431991447392	17.393670939711285	28.990212087729418	26.951797058085376
13	27.84942977181094	20.004758691946403	34.97051892557862	17.175292610664034
14	20.059842180915037	30.263325152294442	26.92800359835336	22.74882906843716
15	16.474526330885542	21.642922097605336	39.049304611694	22.833246959815128
16	19.463375998591946	22.372370904184063	27.779027206028545	30.385225891195443
17	19.30953335484523	28.056400277699012	31.082080917319356	21.551985450136403
18	19.038027163656633	24.020964319588536	30.16032880605723	26.7806797106976
19	19.468916941269267	26.141189738174162	26.39476935011261	27.99512397044396
20	25.912381399381367	24.767687829808317	33.31410300286499	16.00582776794532
21	21.987438357012714	32.67037583888243	27.987627400939353	17.354558403165505
22	18.64462023356703	21.948651758271488	34.77528218065429	24.631445827507196
23	24.031068391529526	26.8605344728119	29.93771328555085	19.17068385010772
24	24.03237214274772	24.44533534111021	27.09292812745472	24.42936438868735
25	19.204907319585278	33.73814808658212	24.936197674759704	22.120746919072904
26	18.059561874403126	23.24588422037307	33.436003741765994	25.25855016345781
27	24.97693990032822	25.06266154292438	25.56069450827393	24.39970404847347
28	15.528328884282303	28.637547383208332	31.424641549899444	24.409482182609914
29	21.321221484516325	24.348205875354864	31.030908682005297	23.299663958123514
30	18.62147864944411	29.1945750911811	27.133344415218687	25.050601844156102
31	23.08161156688081	21.80980225353398	23.533687301789072	31.574898877796137
32	26.680290866896776	25.154250066002405	28.19492384463197	19.97053522246885
33	24.386340598486996	19.972490849296136	24.78594034686301	30.855228205353853
34	24.438816585019246	23.59496360904412	26.983087087321998	24.983132718614634
35	21.50016133921325	21.300035527220697	29.640783945607502	27.559019187958555
36	19.78833598972644	30.07428122565652	26.38531715378071	23.752065630836324
37	18.942853324728574	22.485471322362265	32.81672191312454	25.75495343978462
38	18.120186306049078	23.54313949812097	24.519649160547186	33.81702503528277
39	24.937501425977896	23.54965825421193	30.24768013767613	21.265160182134046
40	21.034396216513965	19.78116535802638	30.22812386940324	28.956314556056412
41	21.177156974906048	23.218179506986477	27.546307613581178	28.0583559045263
42	19.82353727261764	21.27819769431597	34.769415300172426	24.12884973289397
43	22.082612195940772	22.56011107960379	28.915246392683347	26.44203033177209
44	21.95680020338519	20.27267956728497	30.542979788596742	27.2275404407331
45	20.514851356064238	19.277591449999512	28.423406245620207	31.784150948316043
46	26.634985512064585	24.732812484721663	27.36345650522967	21.268745497984074
47	16.161951976323877	23.32052397761459	36.27785545962119	24.239668586440335
48	21.829358521806867	22.937547057270532	27.802820665760557	27.430273755162037
49	21.25179673214757	18.367247161896564	37.22079352817895	23.16016257777691
50	21.282434885775096	20.201951063698026	30.755817174966673	27.759796875560205
51	19.399818126705064	19.914799857891115	29.002923662106795	31.682458353297022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	252.0
2	414.0
3	2259.5
4	4105.0
5	2769.0
6	1433.0
7	1343.0
8	1253.0
9	1222.5
10	1192.0
11	1156.0
12	1120.0
13	1129.0
14	1138.0
15	1052.0
16	966.0
17	940.0
18	914.0
19	891.5
20	869.0
21	1010.0
22	1151.0
23	1198.5
24	1246.0
25	1410.0
26	1740.5
27	1907.0
28	2233.5
29	2560.0
30	3152.0
31	3744.0
32	3966.0
33	4188.0
34	5041.5
35	5895.0
36	6013.0
37	6131.0
38	6866.5
39	7602.0
40	8784.0
41	9966.0
42	10797.0
43	11628.0
44	13406.0
45	15184.0
46	34581.5
47	53979.0
48	40600.5
49	27222.0
50	27514.0
51	27806.0
52	24364.5
53	20923.0
54	19791.0
55	18659.0
56	17255.5
57	15852.0
58	14997.0
59	14142.0
60	12899.0
61	11656.0
62	10790.5
63	9925.0
64	8138.5
65	6352.0
66	5377.0
67	4402.0
68	3619.0
69	2836.0
70	2558.0
71	2280.0
72	1907.0
73	1534.0
74	1408.0
75	1051.0
76	820.0
77	628.0
78	436.0
79	364.0
80	292.0
81	195.0
82	98.0
83	64.5
84	31.0
85	17.0
86	3.0
87	2.0
88	1.0
89	2.0
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	306807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.394190390084994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2807426893467	24.471510732948726
2	9.286503788456947	6.202305511399538
3	3.484759401767624	3.4911215677880016
4	2.14056672634132	2.8592997120849204
5	1.589852466364258	2.6545917976957116
6	1.340026704964056	2.6849464148020763
7	1.1151895697937069	2.606859697030962
8	0.9513086326656253	2.5414545279173852
9	0.7984197428940922	2.3996322814867224
>10	5.890718877398826	31.77151848922719
>50	0.07094843770888831	1.6274519028634866
>100	0.037972403280813465	2.2895114836059838
>500	0.0019985475410954456	0.4227971213713426
>1k	0.007994190164381782	5.036858524056073
>5k	0.002997821311643168	8.940140235721893
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC	9529	3.105861339539189	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	8633	2.813821066664059	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	8629	2.812517315445867	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3457	1.1267669903229065	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2074	0.6759950066328343	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	1935	0.6306896518006434	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCTCGTTT	1864	0.6075480676777257	No Hit
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1622	0.5286711189770767	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTA	1573	0.5127001665542181	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC	1479	0.4820620129266933	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1090	0.35527220695746836	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	762	0.24836460706567973	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATG	505	0.16459859129680873	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	393	0.1280935571874175	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	388	0.1264638681646768	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACCTCGTT	384	0.12516011694648427	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	383	0.12483417914193613	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTAT	358	0.11668573402823273	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGT	339	0.11049291574181815	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	332	0.1082113511099812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.259378045481361E-4	0.0	0.0	0.2623799326612496	0.0
2	3.259378045481361E-4	0.0	0.0	2.0243997040484736	0.0
3	3.259378045481361E-4	0.0	0.0	2.3910797341651264	0.0
4	3.259378045481361E-4	0.0	0.0	3.3721525258550162	0.0
5	3.259378045481361E-4	0.0	0.0	7.32186684136933	0.0
6	3.259378045481361E-4	0.0	0.0	8.144207922244277	0.0
7	3.259378045481361E-4	0.0	0.0	9.456107585550525	0.0
8	3.259378045481361E-4	0.0	0.0	10.79147477078424	0.0
9	3.259378045481361E-4	0.0	0.0	11.136642905800715	0.0
10	3.259378045481361E-4	0.0	0.0	14.827888542308356	0.0
11	3.259378045481361E-4	0.0	0.0	15.957588972872196	0.0
12	3.259378045481361E-4	0.0	0.0	20.109384727206354	0.0
13	3.259378045481361E-4	0.0	0.0	20.675538693706468	0.0
14	3.259378045481361E-4	0.0	0.0	20.980290540958975	0.0
15	3.259378045481361E-4	0.0	0.0	21.84532947422973	0.0
16	3.259378045481361E-4	0.0	0.0	22.51285009794431	0.0
17	3.259378045481361E-4	0.0	0.0	23.153317883881396	0.0
18	3.259378045481361E-4	0.0	0.0	23.768362521063732	0.0
19	3.259378045481361E-4	0.0	0.0	24.93489392354151	0.0
20	3.259378045481361E-4	0.0	0.0	25.528752603428213	0.0
21	3.259378045481361E-4	0.0	0.0	26.084476560182786	0.0
22	3.259378045481361E-4	0.0	0.0	26.70603995345608	0.0
23	3.259378045481361E-4	0.0	0.0	27.229822005364937	0.0
24	3.259378045481361E-4	0.0	0.0	27.642785203727424	0.0
25	3.259378045481361E-4	0.0	0.0	27.9967536594667	0.0
26	3.259378045481361E-4	0.0	0.0	28.340292105460435	0.0
27	3.259378045481361E-4	0.0	0.0	28.79595315621873	0.0
28	3.259378045481361E-4	0.0	0.0	29.14568442049888	0.0
29	3.259378045481361E-4	0.0	0.0	29.501608503065444	0.0
30	3.259378045481361E-4	0.0	0.0	29.884911361214055	0.0
31	3.259378045481361E-4	0.0	0.0	30.265932654730825	0.0
32	3.259378045481361E-4	0.0	0.0	30.629353306801995	0.0
33	3.259378045481361E-4	0.0	0.0	30.96637299670477	0.0
34	3.259378045481361E-4	0.0	0.0	31.3112151939167	0.0
35	3.259378045481361E-4	0.0	0.0	31.641716127728508	0.0
36	3.259378045481361E-4	0.0	0.0	31.965046429840257	0.0
37	3.259378045481361E-4	0.0	0.0	32.2909842343884	0.0
38	3.259378045481361E-4	0.0	0.0	32.65375301085047	0.0
39	3.259378045481361E-4	0.0	0.0	33.01717366292164	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	30	2.160281E-6	45.000004	34
ATACTCC	30	2.160281E-6	45.000004	28
CCCCGTA	30	2.160281E-6	45.000004	40
CACAACG	95	0.0	45.000004	12
ACTGCGA	25	3.884099E-5	45.0	30
GTTTCGA	20	7.0251944E-4	45.0	44
ACGTTAT	20	7.0251944E-4	45.0	21
GCGCGAA	20	7.0251944E-4	45.0	13
TCGATCA	20	7.0251944E-4	45.0	17
TCCTACG	20	7.0251944E-4	45.0	44
ACGGGCC	20	7.0251944E-4	45.0	5
CGTTATT	25	3.884099E-5	45.0	1
AAACGCC	20	7.0251944E-4	45.0	5
CCCGTAA	20	7.0251944E-4	45.0	41
TCGTGAA	20	7.0251944E-4	45.0	43
GAAACGC	20	7.0251944E-4	45.0	4
GCGATTA	25	3.884099E-5	45.0	9
CCGACGG	20	7.0251944E-4	45.0	40
CGATCGA	55	1.8189894E-12	45.0	41
CGATCAC	50	2.1827873E-11	45.0	34
>>END_MODULE