##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	959366
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198498800249332	31.0	31.0	33.0	28.0	34.0
2	31.16064046464019	31.0	31.0	34.0	28.0	34.0
3	30.96811852827805	31.0	31.0	34.0	27.0	34.0
4	34.753148433444586	35.0	35.0	37.0	32.0	37.0
5	34.54957336407586	35.0	35.0	37.0	32.0	37.0
6	34.79006135301856	36.0	35.0	37.0	32.0	37.0
7	35.39432917155705	37.0	35.0	37.0	33.0	37.0
8	35.15314905885762	37.0	35.0	37.0	32.0	37.0
9	37.260747201797855	39.0	37.0	39.0	34.0	39.0
10	36.54445227368908	38.0	35.0	39.0	32.0	39.0
11	36.7919125755968	39.0	37.0	39.0	33.0	39.0
12	36.964953938330105	39.0	37.0	39.0	33.0	39.0
13	37.10991634058326	39.0	37.0	39.0	33.0	39.0
14	38.09433313250626	40.0	37.0	41.0	33.0	41.0
15	38.264459028149844	40.0	38.0	41.0	34.0	41.0
16	38.23590579611952	40.0	38.0	41.0	34.0	41.0
17	38.07591888809902	40.0	37.0	41.0	33.0	41.0
18	38.00294986480655	39.0	37.0	41.0	33.0	41.0
19	37.858521148341715	39.0	37.0	41.0	34.0	41.0
20	37.895079667196875	39.0	36.0	41.0	34.0	41.0
21	37.59487515713502	39.0	36.0	41.0	33.0	41.0
22	37.78393960177868	39.0	36.0	41.0	33.0	41.0
23	37.86979526062004	39.0	36.0	41.0	34.0	41.0
24	37.777323774242575	39.0	36.0	41.0	33.0	41.0
25	37.28468384328817	39.0	35.0	41.0	32.0	41.0
26	37.50213370079823	39.0	35.0	41.0	33.0	41.0
27	37.644611128599514	39.0	36.0	41.0	33.0	41.0
28	37.534433156897364	39.0	36.0	41.0	33.0	41.0
29	37.573072216442945	40.0	36.0	41.0	33.0	41.0
30	37.14859084020072	39.0	35.0	41.0	32.0	41.0
31	37.11783511193851	39.0	35.0	41.0	32.0	41.0
32	37.056795842254154	39.0	35.0	41.0	32.0	41.0
33	36.94439244250891	39.0	35.0	41.0	31.0	41.0
34	36.725098658905985	40.0	35.0	41.0	31.0	41.0
35	36.63336203284252	40.0	35.0	41.0	30.0	41.0
36	36.4252402107225	39.0	35.0	41.0	30.0	41.0
37	36.493990823106095	40.0	35.0	41.0	30.0	41.0
38	36.251955979261304	39.0	35.0	41.0	29.0	41.0
39	36.25312758634348	39.0	35.0	41.0	30.0	41.0
40	36.20922463376855	39.0	35.0	41.0	29.0	41.0
41	36.07063831738878	39.0	35.0	41.0	29.0	41.0
42	36.173122666427616	39.0	35.0	41.0	29.0	41.0
43	36.14727434576585	39.0	35.0	41.0	29.0	41.0
44	36.10754810989758	39.0	35.0	41.0	29.0	41.0
45	36.110589701948996	39.0	35.0	41.0	29.0	41.0
46	35.971283118226	39.0	35.0	41.0	28.0	41.0
47	35.91760808700746	39.0	35.0	41.0	28.0	41.0
48	35.8482456122064	39.0	35.0	41.0	28.0	41.0
49	35.86130110927425	39.0	35.0	41.0	28.0	41.0
50	35.792552581600766	39.0	35.0	41.0	28.0	41.0
51	34.86435729429644	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	53.0
10	43.0
11	40.0
12	48.0
13	52.0
14	68.0
15	95.0
16	130.0
17	245.0
18	421.0
19	665.0
20	1144.0
21	1588.0
22	2482.0
23	3814.0
24	5985.0
25	9724.0
26	13177.0
27	14621.0
28	13933.0
29	14005.0
30	15285.0
31	18559.0
32	23718.0
33	33573.0
34	56059.0
35	66454.0
36	80632.0
37	126031.0
38	219735.0
39	236936.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.75434401469304	21.47689203077866	24.32158321224642	15.447180742281882
2	36.712683167842094	22.739705180296156	23.92121463549886	16.62639701636289
3	32.15508992397062	21.86975565112793	30.07705088568909	15.898103539212356
4	27.940223022287636	24.858708772251674	28.63922632238374	18.561841883076948
5	24.398925957351	30.4116468584461	27.629496980297404	17.5599302039055
6	25.731055718047124	32.52293702299227	27.259356700154058	14.486650558806547
7	77.16460662562568	6.062128530717161	12.394018549750564	4.379246293906601
8	79.17134857812347	4.99288071497218	11.825309631569182	4.0104610753351695
9	72.63098754802651	8.162057025160365	13.93587014757663	5.271085279236496
10	35.20376998976407	30.977124475955996	21.08590464952896	12.73320088475097
11	25.697387649760362	25.622546556788546	31.27992861952581	17.40013717392528
12	23.960198714567746	23.767154558322893	33.253627916770036	19.01901881033933
13	23.13225609412883	23.72660694667103	34.35977510147327	18.781361857726875
14	19.988721718301463	26.573487073754958	33.3719352155486	20.065855992394976
15	19.647663144201484	26.813645678500176	34.91701811404615	18.62167306325219
16	22.882299351863626	27.143759524519318	32.39681206129882	17.57712906231824
17	22.766076763195695	26.489681727307406	31.461819576678764	19.28242193281813
18	23.019994454671107	26.085664907866235	32.293723146327885	18.60061749113477
19	22.434503620099107	27.709758319556876	31.090011528446908	18.765726531897105
20	23.910999555956746	27.65972527690162	30.783767613194545	17.645507553947084
21	23.628417100460094	26.991054508915262	31.782343756188986	17.59818463443566
22	23.068672435754443	24.510041006247878	31.980182745688296	20.44110381230938
23	21.355353431328606	26.18635640620993	32.55577120723478	19.902518955226682
24	20.89192237373432	26.181040395427814	32.874940325173085	20.052096905664783
25	21.33033691000098	27.526825007348606	30.943769114185827	20.19906896846459
26	21.44812303125189	27.812847234527805	30.86548824953146	19.873541484688847
27	21.695890827900925	27.168254868319288	31.177986295115733	19.957868008664057
28	20.284437847495116	27.96857507979228	31.518106749665925	20.228880323046678
29	22.08812903521701	26.620184580233197	30.833800655849803	20.457885728699996
30	21.9703429139661	26.09890281706877	31.237817475290974	20.692936793674154
31	21.883827444374724	26.92872167660726	30.04015151673084	21.14729936228718
32	22.034968927395802	27.214014255247736	30.3470208450164	20.403995972340066
33	21.31741170731504	26.72014643003817	30.440103151456277	21.522338711190518
34	20.727438746005173	27.139694339803576	31.39156484595035	20.7413020682409
35	21.869234473600272	25.876881190286085	31.144109755817905	21.10977458029574
36	21.080275932230244	27.892170454237487	30.035043976959784	20.992509636572485
37	21.3202260659644	28.352370211160288	29.562127488362105	20.765276234513212
38	20.84324439265098	28.70082950615302	29.842312527231524	20.61361357396447
39	21.686613867908598	25.900542650041796	30.554553736530167	21.85828974551944
40	21.732790196859174	25.620566082183444	31.961733061209173	20.68491065974821
41	20.16863220084931	26.384820808742443	30.602189362558192	22.84435762785006
42	21.065161783928136	27.118743003191693	29.70847413812872	22.107621074751453
43	21.306675450245265	26.56817106297284	29.700448004202773	22.424705482579117
44	21.37849371355666	26.93445462941151	29.29382529712331	22.39322635990852
45	22.018291246510717	25.728032888386704	29.405044581525715	22.84863128357686
46	21.258414411184052	26.466437209573822	29.957075818822016	22.31807256042011
47	20.94518671706106	26.563793171740503	30.520468726221274	21.970551384977163
48	20.55023838660115	26.044700354192248	31.06822630779077	22.33683495141583
49	21.469491309885903	25.666012762595297	30.782412551622635	22.082083375896165
50	20.426719312545995	25.462753526808328	31.280241326042407	22.83028583460327
51	20.655516247188245	24.599058127972015	30.274264462155216	24.471161162684524
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	631.0
2	1047.0
3	8206.0
4	15365.0
5	10764.0
6	6163.0
7	6101.0
8	6039.0
9	5925.0
10	5811.0
11	5727.5
12	5644.0
13	5441.5
14	5239.0
15	5007.0
16	4775.0
17	4632.5
18	4490.0
19	4500.5
20	4511.0
21	4939.0
22	5367.0
23	5727.0
24	6087.0
25	7153.5
26	10058.0
27	11896.0
28	13933.5
29	15971.0
30	17879.0
31	19787.0
32	22599.0
33	25411.0
34	27915.0
35	30419.0
36	32880.5
37	35342.0
38	38202.5
39	41063.0
40	45451.5
41	49840.0
42	52916.0
43	55992.0
44	59229.5
45	62467.0
46	69494.0
47	76521.0
48	81640.0
49	86759.0
50	83988.5
51	81218.0
52	73244.0
53	65270.0
54	57726.5
55	50183.0
56	44776.0
57	39369.0
58	36456.0
59	33543.0
60	31194.0
61	28845.0
62	26118.5
63	23392.0
64	20219.0
65	17046.0
66	13779.5
67	10513.0
68	8720.0
69	6927.0
70	5954.5
71	4982.0
72	4016.0
73	3050.0
74	2564.5
75	1707.5
76	1336.0
77	969.5
78	603.0
79	489.5
80	376.0
81	232.0
82	88.0
83	77.0
84	66.0
85	45.5
86	25.0
87	18.0
88	11.0
89	6.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	959366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.29616642425908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31272858611577	25.076251448683646
2	8.392810854285353	5.588968539432262
3	3.048879661383404	3.045480138388814
4	1.7522312403319695	2.333703319675166
5	1.22616095423131	2.041322959750701
6	0.9610388705035668	1.9199346103481234
7	0.7961593688046349	1.8556338390766525
8	0.6818829657697186	1.8163270968108725
9	0.6003483794258992	1.7990369598509077
>10	7.062549898488046	47.88406245330014
>50	0.1295139859074074	2.736007771712292
>100	0.0319045903543975	1.911404742337391
>500	0.0015794351660501445	0.33362547970472295
>1k	0.0018953221992601733	0.7432948502753081
>5k	3.158870332100289E-4	0.9149457906530218
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8699	0.9067446626209393	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	1537	0.16020997200234322	Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT	1320	0.1375908673019473	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGC	1099	0.11455482057942433	Illumina PCR Primer Index 2 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCC	1069	0.11142775541347098	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTG	1030	0.10736257069773163	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTT	1012	0.10548633159815962	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0423550553177828E-4	0.0	0.0	0.14374076212832224	0.0
2	1.0423550553177828E-4	0.0	0.0	0.5346239078724908	0.0
3	1.0423550553177828E-4	0.0	0.0	0.8085548164100042	0.0
4	1.0423550553177828E-4	0.0	0.0	1.1670207199337896	0.0
5	1.0423550553177828E-4	0.0	0.0	1.9501420729940397	0.0
6	1.0423550553177828E-4	0.0	0.0	2.7353481361649257	0.0
7	1.0423550553177828E-4	0.0	0.0	3.3624289374441036	0.0
8	1.0423550553177828E-4	0.0	0.0	4.26667194793228	0.0
9	1.0423550553177828E-4	0.0	0.0	4.7041483646491535	0.0
10	1.0423550553177828E-4	0.0	0.0	5.464025199975818	0.0
11	1.0423550553177828E-4	0.0	0.0	6.395369441902257	0.0
12	1.0423550553177828E-4	0.0	0.0	7.095832039075806	0.0
13	1.0423550553177828E-4	0.0	0.0	7.434597432054086	0.0
14	1.0423550553177828E-4	0.0	0.0	7.60543942562067	0.0
15	1.0423550553177828E-4	0.0	0.0	7.845389559354824	0.0
16	1.0423550553177828E-4	0.0	0.0	8.25951722283258	0.0
17	1.0423550553177828E-4	0.0	0.0	8.79184794958337	0.0
18	1.0423550553177828E-4	0.0	0.0	9.364622052480492	0.0
19	1.0423550553177828E-4	0.0	0.0	9.738723281834044	0.0
20	1.0423550553177828E-4	0.0	0.0	10.111782156132278	0.0
21	1.0423550553177828E-4	0.0	0.0	10.577923336870391	0.0
22	1.0423550553177828E-4	0.0	0.0	11.070331865002512	0.0
23	1.0423550553177828E-4	0.0	0.0	11.57483171177632	0.0
24	1.0423550553177828E-4	0.0	0.0	11.974157933468561	0.0
25	1.0423550553177828E-4	0.0	0.0	12.341275383951485	0.0
26	1.0423550553177828E-4	0.0	0.0	12.684835610184226	0.0
27	1.0423550553177828E-4	0.0	0.0	13.0462201078629	0.0
28	1.0423550553177828E-4	0.0	0.0	13.403643656331369	0.0
29	1.0423550553177828E-4	0.0	0.0	13.791816678931712	0.0
30	1.0423550553177828E-4	0.0	0.0	14.23252439632007	0.0
31	1.0423550553177828E-4	0.0	0.0	14.642169933059959	0.0
32	2.0847101106355657E-4	0.0	0.0	15.050668879238998	0.0
33	2.0847101106355657E-4	0.0	0.0	15.459167825418037	0.0
34	2.0847101106355657E-4	0.0	0.0	15.852239916778373	0.0
35	2.0847101106355657E-4	0.0	0.0	16.25135766745955	0.0
36	2.0847101106355657E-4	0.0	0.0	16.653081305779025	0.0
37	2.0847101106355657E-4	0.0	0.0	17.06147601645253	0.0
38	2.0847101106355657E-4	0.0	0.0	17.46757754600434	0.0
39	2.0847101106355657E-4	0.0	0.0	17.904637020699088	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.0327026E-4	45.0	21
ACCGAAT	20	7.0327026E-4	45.0	24
GTTAACG	20	7.0327026E-4	45.0	1
CGTAGAC	20	7.0327026E-4	45.0	43
TATCGCG	25	3.8903214E-5	45.0	1
TAGTCCG	25	3.8903214E-5	45.0	1
GATAGCG	45	3.8380676E-10	45.0	1
TAGCGCG	30	2.165125E-6	44.999996	1
CGTTTTT	6395	0.0	43.487095	1
CGCACGG	55	6.002665E-11	40.909092	2
CGACGGT	50	1.0804797E-9	40.5	28
TCGTTAG	40	3.457426E-7	39.375	1
TATTGCG	40	3.457426E-7	39.375	1
ATTGCGG	115	0.0	39.130432	2
CGGCGAT	35	6.24721E-6	38.571426	28
TCGATTG	65	9.094947E-12	38.07692	1
TCAAGCG	155	0.0	37.741936	17
GTCGAGA	30	1.1397814E-4	37.499996	9
CGACGTT	30	1.1397814E-4	37.499996	27
TCGATAG	30	1.1397814E-4	37.499996	1
>>END_MODULE