##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988267
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36328239230896	31.0	31.0	33.0	28.0	34.0
2	31.315582732196866	31.0	31.0	34.0	28.0	34.0
3	31.128741524304665	31.0	31.0	34.0	28.0	34.0
4	34.884885359928035	35.0	35.0	37.0	32.0	37.0
5	34.62259288228788	35.0	35.0	37.0	32.0	37.0
6	34.91106249626872	37.0	35.0	37.0	32.0	37.0
7	35.52356701174885	37.0	35.0	37.0	33.0	37.0
8	35.39106840560294	37.0	35.0	37.0	33.0	37.0
9	37.49563528884401	39.0	37.0	39.0	34.0	39.0
10	36.70391402323461	39.0	35.0	39.0	32.0	39.0
11	36.90426271442839	39.0	37.0	39.0	33.0	39.0
12	37.10070254293627	39.0	37.0	39.0	34.0	39.0
13	37.248970166969045	39.0	37.0	39.0	34.0	39.0
14	38.27281089017442	40.0	38.0	41.0	34.0	41.0
15	38.447296125439784	40.0	38.0	41.0	34.0	41.0
16	38.44278924622597	40.0	38.0	41.0	34.0	41.0
17	38.294878813114266	40.0	38.0	41.0	34.0	41.0
18	38.211981175127775	39.0	38.0	41.0	34.0	41.0
19	38.06130630689884	39.0	37.0	41.0	34.0	41.0
20	38.074084230273805	40.0	37.0	41.0	34.0	41.0
21	37.810992373518495	39.0	36.0	41.0	33.0	41.0
22	37.985591950353495	40.0	36.0	41.0	34.0	41.0
23	38.06535379608952	40.0	36.0	41.0	34.0	41.0
24	37.98743558167985	40.0	36.0	41.0	34.0	41.0
25	37.517567620895974	39.0	35.0	41.0	33.0	41.0
26	37.74441421194879	39.0	36.0	41.0	33.0	41.0
27	37.89469748559853	40.0	36.0	41.0	34.0	41.0
28	37.79853015430041	40.0	36.0	41.0	34.0	41.0
29	37.83002063207615	40.0	36.0	41.0	34.0	41.0
30	37.43260879903912	39.0	36.0	41.0	33.0	41.0
31	37.38709478308999	39.0	36.0	41.0	33.0	41.0
32	37.287376791899355	40.0	36.0	41.0	33.0	41.0
33	37.13132382240832	40.0	36.0	41.0	32.0	41.0
34	36.890635830195684	40.0	36.0	41.0	31.0	41.0
35	36.79973327046233	40.0	36.0	41.0	31.0	41.0
36	36.61060118368821	40.0	36.0	41.0	30.0	41.0
37	36.67431169916632	40.0	36.0	41.0	30.0	41.0
38	36.4551877174893	40.0	35.0	41.0	30.0	41.0
39	36.4619591669053	40.0	35.0	41.0	30.0	41.0
40	36.4134388783598	40.0	35.0	41.0	30.0	41.0
41	36.2805001077644	39.0	35.0	41.0	29.0	41.0
42	36.38127348176151	40.0	35.0	41.0	30.0	41.0
43	36.33872425164454	40.0	35.0	41.0	30.0	41.0
44	36.33487711316881	40.0	35.0	41.0	30.0	41.0
45	36.34070549760338	40.0	35.0	41.0	30.0	41.0
46	36.21290096704635	39.0	35.0	41.0	29.0	41.0
47	36.149357410497366	39.0	35.0	41.0	29.0	41.0
48	36.08794890449646	39.0	35.0	41.0	29.0	41.0
49	36.092822081482026	39.0	35.0	41.0	29.0	41.0
50	36.038255855957956	39.0	35.0	41.0	28.0	41.0
51	35.18654675305358	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	22.0
9	38.0
10	52.0
11	52.0
12	39.0
13	45.0
14	47.0
15	72.0
16	104.0
17	180.0
18	354.0
19	589.0
20	940.0
21	1552.0
22	2391.0
23	3818.0
24	6401.0
25	10623.0
26	14360.0
27	14939.0
28	13508.0
29	12917.0
30	13622.0
31	16427.0
32	21292.0
33	30432.0
34	52298.0
35	61061.0
36	74802.0
37	123118.0
38	231844.0
39	280290.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.76934775723564	22.794346062349547	22.88602169251832	16.550284487896487
2	35.24644655745866	23.551125353775852	25.51102080713006	15.691407281635428
3	30.726311816543507	22.640136724184863	31.61392619605835	15.019625263213282
4	27.337045555502716	25.29852762461966	29.98531773296083	17.379109086916795
5	24.57898523374756	30.20438808540607	28.299032548896196	16.91759413195017
6	26.044479882460912	31.944808437395967	27.93961550876433	14.07109617137879
7	78.67124977359357	4.936115442486696	12.38410267670579	4.0085321072139415
8	81.00007386667774	3.9684619642262673	11.556087575523618	3.4753765935723844
9	74.88300226558208	7.010656027166748	13.284365459941494	4.821976247309685
10	34.675649394343836	31.94885592658664	20.99473118094604	12.380763498123484
11	24.14023740547848	26.09638893133131	32.97701936824765	16.78635429494256
12	22.627387133234237	24.379342829417556	34.329589068541196	18.66368096880701
13	21.66378114416448	24.660238579250343	35.70441995938344	17.971560317201728
14	18.99375371230649	27.907033220779404	33.78550533408482	19.313707732829286
15	18.342209139837713	28.363590001487456	35.7485375915618	17.54566326711304
16	21.592747708868153	28.380994205007354	33.20742269042678	16.81883539569772
17	21.8163714866529	27.304868016436856	32.22550181276922	18.65325868414103
18	21.624722873474475	27.034799300189118	33.52909689385561	17.811380932480798
19	21.086811560033876	28.047278721236264	32.12876682111211	18.737142897617748
20	22.53338419678083	28.116693160856325	32.553651998903135	16.796270643459714
21	22.151807153330022	27.33107550894647	33.615510788076506	16.901606549647006
22	22.241155477214154	24.610656836664585	33.45543259058534	19.692755095535926
23	19.920527549741113	26.827972602545668	33.902073022776236	19.349426824936984
24	19.673428334650453	26.40976578191926	34.587818878906205	19.32898700452408
25	19.66583929241794	28.573351128794144	32.41138275385093	19.349426824936984
26	19.479755976876696	29.709177782927082	31.814884034375325	18.996182205820897
27	19.820959315650526	28.935904973048782	32.52005783862054	18.723077872680154
28	18.092681431232652	28.579624737039687	33.53759662115603	19.790097210571638
29	19.636596183015318	27.793096400061927	32.71302188578593	19.857285531136828
30	20.110253605553964	27.586775638567314	32.59372214189081	19.70924861398792
31	19.631840383216275	28.811647054895083	31.617063000181123	19.939449561707516
32	19.783014104487957	28.859508614574807	31.30166240499784	20.055814875939397
33	20.30342002717889	28.254206606109484	30.96228043635981	20.480092930351816
34	19.48026191302553	27.774579137014594	31.824901570122243	20.920257379837633
35	19.51304657546999	28.460223805914797	30.850164985778132	21.176564632837078
36	20.675687845491147	29.55628387874937	30.081749162928638	19.686279112830846
37	20.223380928433308	28.547447197973824	30.94679879020548	20.282373083387384
38	20.045594965732942	28.74739316399313	30.412024280887657	20.794987589386267
39	21.422449601170534	27.12101081995048	29.991591341206377	21.464948237672612
40	21.263383275977038	27.09105939993949	31.110823289657553	20.53473403442592
41	19.890070193581288	28.203005867847452	30.434083096976828	21.472840841594426
42	21.24537194907854	28.10414594436524	30.140741317882718	20.509740788673504
43	21.057770825090792	28.185399289868023	30.173525980327177	20.583303904714008
44	20.965285697083885	27.753835754912387	29.581175937272015	21.699702610731716
45	20.762202926941807	26.903660650411275	29.779604094844814	22.5545323278021
46	20.617909937294275	27.90106317422316	29.598984889710977	21.882041998771587
47	19.71501628608463	28.17153663938996	30.998707839075877	21.114739235449527
48	20.087992415005257	27.251845908039023	30.93081120790232	21.729350469053404
49	20.90335911246657	26.461573643559888	31.355392824004042	21.279674419969503
50	19.715825783922767	26.38467134893708	31.242973811733066	22.65652905540709
51	19.802340865373427	25.98730909764264	30.04491701129351	24.165433025690426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	227.0
1	631.0
2	1035.0
3	8291.0
4	15547.0
5	11138.0
6	6729.0
7	6721.0
8	6713.0
9	6668.0
10	6623.0
11	6391.5
12	6160.0
13	6197.0
14	6234.0
15	5896.5
16	5559.0
17	5572.5
18	5586.0
19	5566.5
20	5547.0
21	5612.5
22	5678.0
23	6237.0
24	6796.0
25	8023.5
26	10991.5
27	12732.0
28	15129.0
29	17526.0
30	19638.5
31	21751.0
32	25316.0
33	28881.0
34	33470.5
35	38060.0
36	40371.0
37	42682.0
38	47108.0
39	51534.0
40	53639.5
41	55745.0
42	59960.0
43	64175.0
44	68566.5
45	72958.0
46	79045.5
47	85133.0
48	87368.0
49	89603.0
50	87894.0
51	86185.0
52	75086.0
53	63987.0
54	56556.5
55	49126.0
56	42310.5
57	35495.0
58	30932.5
59	26370.0
60	23683.5
61	20997.0
62	18103.5
63	15210.0
64	12288.5
65	9367.0
66	7380.0
67	5393.0
68	4235.5
69	3078.0
70	2492.0
71	1906.0
72	1488.0
73	1070.0
74	922.5
75	560.5
76	346.0
77	239.0
78	132.0
79	188.0
80	244.0
81	153.5
82	63.0
83	47.5
84	32.0
85	23.0
86	14.0
87	11.5
88	9.0
89	5.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	988267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.41549692259055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05654520247084	25.080517552334424
2	8.576359298185025	5.731666154710651
3	3.122407794065283	3.130104241009836
4	1.7368211341740585	2.321469650563339
5	1.1967531358041639	1.9995050363282312
6	0.9199432926205753	1.844421735811239
7	0.7843888855958804	1.834752107492036
8	0.6668730707057758	1.7827116033541888
9	0.5911328672508491	1.777769865581659
>10	7.218502613804685	48.752809727231785
>50	0.09900736459464055	2.070962721394443
>100	0.026360973555262998	1.4512763511725615
>500	0.003065229483115624	0.6402659659168847
>1k	0.001532614741557812	0.7538565844102179
>5k	3.065229483115624E-4	0.8279107026885585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8083	0.8178963782054849	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	1791	0.18122632851243642	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	1560	0.157852078436293	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	1512	0.15299509140748402	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	1361	0.13771581971268898	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	1136	0.11494869301514672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.011872297668545E-4	0.0	0.0	0.15805445289582673	0.0
2	1.011872297668545E-4	0.0	0.0	0.613396986846672	0.0
3	1.011872297668545E-4	0.0	0.0	0.9187800462830389	0.0
4	1.011872297668545E-4	0.0	0.0	1.2834588223627825	0.0
5	1.011872297668545E-4	0.0	0.0	2.1672280871464897	0.0
6	1.011872297668545E-4	0.0	0.0	2.820796404210603	0.0
7	1.011872297668545E-4	0.0	0.0	3.4018134775318813	0.0
8	1.011872297668545E-4	0.0	0.0	4.243387667502811	0.0
9	1.011872297668545E-4	0.0	0.0	4.591775299590091	0.0
10	1.011872297668545E-4	0.0	0.0	5.3176924859375045	0.0
11	1.011872297668545E-4	0.0	0.0	6.277149798586819	0.0
12	1.011872297668545E-4	0.0	0.0	7.048095302180483	0.0
13	2.02374459533709E-4	0.0	0.0	7.393346130144991	0.0
14	2.02374459533709E-4	0.0	0.0	7.548870902296646	0.0
15	2.02374459533709E-4	0.0	0.0	7.788482262384558	0.0
16	2.02374459533709E-4	0.0	0.0	8.24554497924144	0.0
17	2.02374459533709E-4	0.0	0.0	8.845281690069587	0.0
18	2.02374459533709E-4	0.0	0.0	9.472743701853851	0.0
19	2.02374459533709E-4	0.0	0.0	9.916348517151741	0.0
20	2.02374459533709E-4	0.0	0.0	10.344168124606002	0.0
21	2.02374459533709E-4	0.0	0.0	10.863258613309966	0.0
22	2.02374459533709E-4	0.0	0.0	11.41614563675606	0.0
23	2.02374459533709E-4	0.0	0.0	11.990079603993658	0.0
24	2.02374459533709E-4	0.0	0.0	12.423768070774395	0.0
25	2.02374459533709E-4	0.0	0.0	12.815261462742356	0.0
26	2.02374459533709E-4	0.0	0.0	13.172958319968187	0.0
27	2.02374459533709E-4	0.0	0.0	13.546541572267413	0.0
28	2.02374459533709E-4	0.0	0.0	13.937933777005608	0.0
29	2.02374459533709E-4	0.0	0.0	14.350777674454374	0.0
30	2.02374459533709E-4	0.0	0.0	14.807840391311254	0.0
31	2.02374459533709E-4	0.0	0.0	15.253468951204482	0.0
32	2.02374459533709E-4	0.0	0.0	15.66823540601882	0.0
33	2.02374459533709E-4	0.0	0.0	16.071365329409968	0.0
34	2.02374459533709E-4	0.0	0.0	16.456888674821684	0.0
35	2.02374459533709E-4	0.0	0.0	16.88197622707224	0.0
36	2.02374459533709E-4	0.0	0.0	17.302915102902354	0.0
37	2.02374459533709E-4	0.0	0.0	17.703717720008864	0.0
38	2.02374459533709E-4	0.0	0.0	18.12779339996175	0.0
39	2.02374459533709E-4	0.0	0.0	18.569678032353604	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGTT	20	7.032806E-4	45.0	10
AACGCGC	20	7.032806E-4	45.0	5
ACGTGCG	20	7.032806E-4	45.0	1
AGCGCGC	25	3.8904076E-5	45.0	25
GTCAACG	20	7.032806E-4	45.0	1
CCGCATT	20	7.032806E-4	45.0	21
CGTTTTT	6875	0.0	43.429092	1
TTTAGCG	60	3.6379788E-12	41.249996	1
TATTGCG	55	6.002665E-11	40.909092	1
CGCCTAA	55	6.002665E-11	40.909092	14
CTACGAG	50	1.0804797E-9	40.5	1
GAGTACG	45	1.927765E-8	40.0	1
CGCCGGT	210	0.0	39.64286	28
GCGTAAC	40	3.4575714E-7	39.375	22
TAGTACG	40	3.4575714E-7	39.375	1
CGTTATT	180	0.0	38.75	1
ATTAGCG	70	0.0	38.571426	1
CGAAACG	35	6.247401E-6	38.571426	2
GGCGCCG	35	6.247401E-6	38.571426	43
GCGTCCC	35	6.247401E-6	38.571426	7
>>END_MODULE