FastQCFastQC Report
Sat 18 Jun 2016
SRR3551740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690646
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC18930.27409121315406154No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.25338596039070665No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT12000.17375037283934172No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC10110.1463846891171454No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC7280.10540855952253397No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG253.889182E-545.0000041
CCTGTCG253.889182E-545.00000439
ACGATAG302.164239E-645.0000041
TATACGG502.1827873E-1145.0000042
TCGGCAT207.031329E-445.032
TGTTACG207.031329E-445.01
GTATACG406.8084773E-945.01
GCCACGC207.031329E-445.011
GTGCGAA207.031329E-445.034
CGAAGTA207.031329E-445.019
TACGGGT603.6379788E-1241.2500044
TAAGCGG800.039.3752
TACGTGT403.4555524E-739.37533
CACGACC1950.039.2307727
CGTTTTT9600.039.140631
ACAACGA2950.038.89830413
CACAACG2950.038.89830412
CATACGA1450.038.79310218
GCGAATG356.244667E-638.5714261
TAGTAAC356.244667E-638.57142611