##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690646
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173331924024755	31.0	31.0	33.0	28.0	34.0
2	31.09697587476073	31.0	31.0	34.0	28.0	34.0
3	30.920103786889378	31.0	31.0	34.0	27.0	34.0
4	34.7935440153132	35.0	35.0	37.0	32.0	37.0
5	34.5420157359921	35.0	35.0	37.0	32.0	37.0
6	34.75572435082517	36.0	35.0	37.0	32.0	37.0
7	35.39810988552746	37.0	35.0	37.0	33.0	37.0
8	35.11280598164617	37.0	35.0	37.0	32.0	37.0
9	37.04018846123774	39.0	37.0	39.0	32.0	39.0
10	36.404251961207336	37.0	35.0	39.0	32.0	39.0
11	36.57804866747943	38.0	35.0	39.0	32.0	39.0
12	36.55764168618945	39.0	35.0	39.0	33.0	39.0
13	36.62062909218326	39.0	35.0	39.0	33.0	39.0
14	37.480606562551586	39.0	36.0	41.0	33.0	41.0
15	37.670732908031034	39.0	36.0	41.0	33.0	41.0
16	37.64758935836883	39.0	36.0	41.0	33.0	41.0
17	37.46258285720905	39.0	36.0	41.0	33.0	41.0
18	37.44892318206433	39.0	36.0	41.0	33.0	41.0
19	37.412910521453824	39.0	35.0	41.0	33.0	41.0
20	37.551938330201	39.0	35.0	41.0	33.0	41.0
21	37.25995227656426	39.0	35.0	41.0	32.0	41.0
22	37.47321058834772	39.0	35.0	41.0	33.0	41.0
23	37.59958792203241	39.0	35.0	41.0	33.0	41.0
24	37.50761750592923	39.0	35.0	41.0	33.0	41.0
25	37.06782490595761	39.0	35.0	41.0	32.0	41.0
26	37.240742724927095	39.0	35.0	41.0	33.0	41.0
27	37.36965102237615	39.0	35.0	41.0	33.0	41.0
28	37.26790135612166	39.0	35.0	41.0	33.0	41.0
29	37.33136947148032	39.0	35.0	41.0	33.0	41.0
30	36.96279714933555	39.0	35.0	41.0	32.0	41.0
31	36.94728558480032	39.0	35.0	40.0	32.0	41.0
32	37.0013798675443	39.0	35.0	40.0	32.0	41.0
33	37.07427104478995	39.0	35.0	41.0	32.0	41.0
34	36.93292656440492	39.0	35.0	41.0	32.0	41.0
35	36.919329149810466	39.0	35.0	41.0	32.0	41.0
36	36.804021162795415	39.0	35.0	41.0	31.0	41.0
37	36.92075535078752	39.0	35.0	41.0	32.0	41.0
38	36.68222070351526	39.0	35.0	41.0	31.0	41.0
39	36.72486049292981	39.0	35.0	41.0	32.0	41.0
40	36.62824804603227	39.0	35.0	40.0	31.0	41.0
41	36.500418448814585	38.0	35.0	40.0	31.0	41.0
42	36.59386429516714	39.0	35.0	40.0	31.0	41.0
43	36.56737025914868	39.0	35.0	40.0	31.0	41.0
44	36.54129322402504	39.0	35.0	40.0	31.0	41.0
45	36.53818598819077	38.0	35.0	40.0	31.0	41.0
46	36.3980143228224	38.0	35.0	40.0	31.0	41.0
47	36.33214700439878	38.0	35.0	40.0	31.0	41.0
48	36.279519464385515	38.0	35.0	40.0	31.0	41.0
49	36.313292193106165	38.0	35.0	40.0	31.0	41.0
50	36.25095345517096	38.0	35.0	40.0	31.0	41.0
51	35.31902161165054	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	28.0
9	52.0
10	48.0
11	50.0
12	46.0
13	45.0
14	69.0
15	67.0
16	108.0
17	151.0
18	247.0
19	412.0
20	664.0
21	951.0
22	1443.0
23	2030.0
24	2930.0
25	3870.0
26	5222.0
27	6295.0
28	7530.0
29	9019.0
30	11573.0
31	15069.0
32	20880.0
33	31811.0
34	61795.0
35	61373.0
36	58679.0
37	88971.0
38	148489.0
39	150702.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93523165268459	22.324606238217555	24.57756940603435	11.16259270306351
2	33.129417965209385	24.11365591055331	24.165925814382476	18.591000309854834
3	33.757380771046236	23.13674443926411	26.173032204631607	16.932842585058047
4	29.906058965084863	25.851594014878827	24.260330183625186	19.98201683641113
5	25.477017169432674	30.189561656767722	23.815094853224373	20.51832632057523
6	27.342082629885645	32.91715292639065	23.986528554425856	15.754235889297846
7	78.35504730353901	7.023134862143558	9.370502399203065	5.251315435114371
8	79.43852567016967	5.671067377498748	9.307517889048803	5.5828890632827814
9	73.07984119215922	8.227804113829661	11.63664163696018	7.0557130570509345
10	45.01741847487715	22.797062460363197	17.772925637736265	14.412593427023396
11	35.7023714030053	23.580821433845994	22.196030962316442	18.520776200832266
12	32.52462187575111	22.037628539077907	26.437711939256868	19.000037645914112
13	28.193604248775785	22.945184653208734	27.54812161367763	21.31308948433785
14	22.3385062680447	25.08825071020465	29.56594261025185	23.0073004114988
15	23.19712269382578	24.474477518149673	32.334075633537296	19.99432415448725
16	28.452492304306404	24.367331454898746	28.854434833474745	18.325741407320102
17	26.339108602670542	24.998914060169756	26.939271348853104	21.7227059883066
18	27.2033719155689	25.402738885043856	27.984669425436476	19.409219773950763
19	26.56599763120325	28.22690640356999	25.443715014638467	19.76338095058829
20	28.94377148350964	27.722306362449068	25.88750242526562	17.446419728775666
21	27.357430579486454	28.384584866921692	26.056764246806612	18.201220306785242
22	25.22609267265719	24.907405530474367	28.238924137691377	21.62757765917706
23	25.57156633065275	25.164556082276597	28.9020713940282	20.361806193042455
24	27.842194119708214	22.43841273242732	28.943047523622813	20.776345624241653
25	24.90016593160606	24.345757450271197	25.884027417808834	24.87004920031391
26	22.22151435033288	25.35626066030933	28.08269359411334	24.33953139524445
27	22.51674519218239	25.362631507313445	28.84386501912702	23.27675828137715
28	20.206733985283343	28.330143083432034	28.152628119181173	23.310494812103453
29	23.93860240991767	27.07436226373569	27.295170029219022	21.691865297127617
30	27.0273048710917	24.224132189283658	28.23993768153294	20.5086252580917
31	26.87252224728732	25.344242926187942	26.614068567688804	21.16916625883593
32	27.55318933288544	25.27054380970859	26.70818335297678	20.468083504429185
33	27.63977493535038	25.356695036241433	26.338529434761078	20.665000593647108
34	23.013526466525544	26.387034747178728	29.999594582463374	20.599844203832355
35	21.94351375378993	27.428233856418483	28.566733174448274	22.061519215343317
36	23.689270624893215	31.307500514011522	25.375952369231126	19.62727649186414
37	24.280021892546976	30.75627745617871	24.79533653999299	20.168364111281324
38	23.377099121691867	29.80166394940389	24.72076867164944	22.100468257254803
39	24.951422291593666	26.506053752573678	26.26902928562534	22.273494670207313
40	24.551217266153717	24.47288480639865	27.058869522157515	23.917028405290118
41	21.31439261213415	26.09368620103497	25.79165013624925	26.80027105058163
42	22.237586259820517	26.0431538009342	25.34062312675379	26.378636812491496
43	22.967916993655216	23.969732685051387	26.7119479443883	26.3504023769051
44	22.103074512847392	24.402515905398715	27.394207741737446	26.100201840016446
45	23.52377339476374	24.715845744418992	26.510252719917293	25.250128140899967
46	22.932153375245782	26.431920260162222	26.631877980904832	24.004048383687156
47	21.02336073762825	25.977852619142077	28.62537392528155	24.373412717948124
48	21.36506980421229	23.828994883051518	30.3263900753787	24.479545237357488
49	23.625127778920024	23.218262322521234	29.62038439374151	23.536225504817228
50	20.334150925365527	23.81321255751861	29.891579767348254	25.961056749767607
51	20.11348795185956	23.09113496639378	29.822079618212516	26.97329746353414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	662.0
2	1083.0
3	2320.5
4	3558.0
5	2414.5
6	1271.0
7	1265.0
8	1259.0
9	1233.0
10	1207.0
11	1154.5
12	1102.0
13	1225.5
14	1349.0
15	1237.0
16	1125.0
17	1228.5
18	1332.0
19	1466.5
20	1601.0
21	2019.0
22	2437.0
23	2534.0
24	2631.0
25	3100.0
26	4784.5
27	6000.0
28	7009.5
29	8019.0
30	9289.0
31	10559.0
32	12389.5
33	14220.0
34	15650.5
35	17081.0
36	18244.5
37	19408.0
38	20172.5
39	20937.0
40	23387.0
41	25837.0
42	29249.0
43	32661.0
44	35576.5
45	38492.0
46	43600.0
47	48708.0
48	53347.5
49	57987.0
50	58333.5
51	58680.0
52	56024.5
53	53369.0
54	50057.0
55	46745.0
56	44919.5
57	43094.0
58	42473.0
59	41852.0
60	40293.0
61	38734.0
62	35711.5
63	32689.0
64	27258.0
65	21827.0
66	16917.0
67	12007.0
68	9310.0
69	6613.0
70	5486.5
71	4360.0
72	3765.5
73	3171.0
74	2483.5
75	1510.0
76	1224.0
77	827.5
78	431.0
79	292.5
80	154.0
81	121.0
82	88.0
83	87.5
84	87.0
85	58.0
86	29.0
87	23.5
88	18.0
89	11.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	690646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.209803861386895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.748923971653	21.541916042003187
2	9.133724496519022	5.335886021345308
3	3.1331385169756047	2.7455508465424208
4	1.6011593046254615	1.870781969557775
5	1.0737619288526712	1.5682187667805498
6	0.8136265835200253	1.425952375261816
7	0.691007383850641	1.4128933104333117
8	0.6498019000902852	1.5184468840315004
9	0.5812451176119646	1.528025029275082
>10	8.283113115294624	52.84316222860848
>50	0.23139642866417254	4.264781390869331
>100	0.055094387776978504	2.7129808964370947
>500	0.002003432282799218	0.374966028773095
>1k	0.002003432282799218	0.8564382100810372
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1893	0.27409121315406154	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1750	0.25338596039070665	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	1200	0.17375037283934172	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1011	0.1463846891171454	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC	728	0.10540855952253397	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17939725995662031	0.0
2	0.0	0.0	0.0	0.7928808680568628	0.0
3	1.447919773661181E-4	0.0	0.0	1.0692887528487822	0.0
4	1.447919773661181E-4	0.0	0.0	1.4499468613443067	0.0
5	1.447919773661181E-4	0.0	0.0	2.4365593951170355	0.0
6	1.447919773661181E-4	0.0	0.0	3.0278898306802615	0.0
7	1.447919773661181E-4	0.0	0.0	3.613283795171477	0.0
8	1.447919773661181E-4	0.0	0.0	4.4913023459196175	0.0
9	1.447919773661181E-4	0.0	0.0	4.852703121425448	0.0
10	1.447919773661181E-4	0.0	0.0	5.61575684214489	0.0
11	1.447919773661181E-4	0.0	0.0	6.726021724588284	0.0
12	1.447919773661181E-4	0.0	0.0	7.537580757725376	0.0
13	1.447919773661181E-4	0.0	0.0	7.877552320581021	0.0
14	1.447919773661181E-4	0.0	0.0	8.031900568453302	0.0
15	1.447919773661181E-4	0.0	0.0	8.349863750749298	0.0
16	1.447919773661181E-4	0.0	0.0	8.908326407450415	0.0
17	1.447919773661181E-4	0.0	0.0	9.54338402017821	0.0
18	1.447919773661181E-4	0.0	0.0	10.233462584305128	0.0
19	1.447919773661181E-4	0.0	0.0	10.703167758880817	0.0
20	1.447919773661181E-4	0.0	0.0	11.168818758090252	0.0
21	1.447919773661181E-4	0.0	0.0	11.699191771182342	0.0
22	1.447919773661181E-4	0.0	0.0	12.207411611737417	0.0
23	1.447919773661181E-4	0.0	0.0	12.720843963477671	0.0
24	1.447919773661181E-4	0.0	0.0	13.158115735123348	0.0
25	1.447919773661181E-4	0.0	0.0	13.585686444285495	0.0
26	1.447919773661181E-4	0.0	0.0	13.954182026682265	0.0
27	1.447919773661181E-4	0.0	0.0	14.31355571450497	0.0
28	1.447919773661181E-4	0.0	0.0	14.693200279158932	0.0
29	1.447919773661181E-4	0.0	0.0	15.0625646134199	0.0
30	1.447919773661181E-4	0.0	0.0	15.491438450378341	0.0
31	2.895839547322362E-4	0.0	0.0	15.902358082143385	0.0
32	2.895839547322362E-4	0.0	0.0	16.291124541371413	0.0
33	2.895839547322362E-4	0.0	0.0	16.73317444827017	0.0
34	2.895839547322362E-4	0.0	0.0	17.16060036545495	0.0
35	2.895839547322362E-4	0.0	0.0	17.568479365695307	0.0
36	2.895839547322362E-4	0.0	0.0	17.983163588871868	0.0
37	2.895839547322362E-4	0.0	0.0	18.390608213180123	0.0
38	2.895839547322362E-4	0.0	0.0	18.89433950243685	0.0
39	2.895839547322362E-4	0.0	0.0	19.437164625582426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.889182E-5	45.000004	1
CCTGTCG	25	3.889182E-5	45.000004	39
ACGATAG	30	2.164239E-6	45.000004	1
TATACGG	50	2.1827873E-11	45.000004	2
TCGGCAT	20	7.031329E-4	45.0	32
TGTTACG	20	7.031329E-4	45.0	1
GTATACG	40	6.8084773E-9	45.0	1
GCCACGC	20	7.031329E-4	45.0	11
GTGCGAA	20	7.031329E-4	45.0	34
CGAAGTA	20	7.031329E-4	45.0	19
TACGGGT	60	3.6379788E-12	41.250004	4
TAAGCGG	80	0.0	39.375	2
TACGTGT	40	3.4555524E-7	39.375	33
CACGACC	195	0.0	39.23077	27
CGTTTTT	960	0.0	39.14063	1
ACAACGA	295	0.0	38.898304	13
CACAACG	295	0.0	38.898304	12
CATACGA	145	0.0	38.793102	18
GCGAATG	35	6.244667E-6	38.571426	1
TAGTAAC	35	6.244667E-6	38.571426	11
>>END_MODULE