FastQCFastQC Report
Sat 18 Jun 2016
SRR3551739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600872
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC24700.4110692460291044No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG24140.40174945745516516No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC21700.36114180724014433No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC19640.32685829927172505No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.28841417140422587No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC11960.19904405597198738No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT9200.15311081228614412Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA302.163766E-645.00000431
AACCGTC207.0305966E-445.039
CGACGTT253.8885755E-545.027
TTAGCCG207.0305966E-445.01
AACCCGA253.8885755E-545.024
TCGATTG207.0305966E-445.01
CGTTCTA207.0305966E-445.023
TACGAAT900.045.012
AGTTACG253.8885755E-545.01
TATAGCG253.8885755E-545.01
ATAACGG453.8380676E-1045.02
CACGTCA253.8885755E-545.034
TCAACCG207.0305966E-445.037
ACGAATG207.0305966E-445.01
ACACGAC1250.043.226
AAACACG1200.043.12500440
CGTTTTT9300.040.645161
CGAATAT1000.040.514
AACGACG501.0804797E-940.513
CACGACC1350.040.00000427