##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600872
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295535488423493	31.0	31.0	33.0	28.0	34.0
2	31.222559879641587	31.0	31.0	34.0	28.0	34.0
3	31.068051099069354	31.0	31.0	34.0	27.0	34.0
4	34.92152738020743	35.0	35.0	37.0	32.0	37.0
5	34.55006224287369	35.0	35.0	37.0	32.0	37.0
6	34.80634810741722	36.0	35.0	37.0	32.0	37.0
7	35.465866274347945	37.0	35.0	37.0	33.0	37.0
8	35.056150061910024	37.0	35.0	37.0	32.0	37.0
9	37.00304390951817	39.0	37.0	39.0	32.0	39.0
10	36.47327717051219	38.0	35.0	39.0	32.0	39.0
11	36.694036333861455	39.0	35.0	39.0	33.0	39.0
12	36.71351302773303	39.0	35.0	39.0	33.0	39.0
13	36.76795057849259	39.0	35.0	39.0	33.0	39.0
14	37.69301282136628	39.0	36.0	41.0	33.0	41.0
15	37.848483537259185	40.0	36.0	41.0	33.0	41.0
16	37.88610386238666	39.0	36.0	41.0	33.0	41.0
17	37.688965370328454	39.0	36.0	41.0	33.0	41.0
18	37.70024397875088	39.0	36.0	41.0	33.0	41.0
19	37.641584230917736	39.0	36.0	41.0	33.0	41.0
20	37.70989162417287	39.0	36.0	41.0	33.0	41.0
21	37.46939947276625	39.0	35.0	41.0	33.0	41.0
22	37.65554727129904	39.0	35.0	41.0	33.0	41.0
23	37.742011609793764	39.0	36.0	41.0	33.0	41.0
24	37.64736749257745	39.0	35.0	41.0	33.0	41.0
25	37.222651413279365	39.0	35.0	41.0	32.0	41.0
26	37.3623184305476	39.0	35.0	41.0	33.0	41.0
27	37.48388675125484	39.0	35.0	41.0	33.0	41.0
28	37.365969790571036	39.0	35.0	41.0	33.0	41.0
29	37.414512575057586	39.0	35.0	41.0	33.0	41.0
30	37.0429708823177	39.0	35.0	41.0	32.0	41.0
31	37.0892519538271	39.0	35.0	41.0	32.0	41.0
32	37.09801089083865	39.0	35.0	41.0	32.0	41.0
33	37.143754410257095	39.0	35.0	41.0	33.0	41.0
34	36.977371220492884	39.0	35.0	41.0	32.0	41.0
35	36.97472506623707	39.0	35.0	41.0	32.0	41.0
36	36.850923324768004	39.0	35.0	41.0	31.0	41.0
37	36.872393787695216	39.0	35.0	41.0	32.0	41.0
38	36.67508720659308	39.0	35.0	41.0	31.0	41.0
39	36.71213669467041	39.0	35.0	41.0	31.0	41.0
40	36.60540680877125	39.0	35.0	41.0	31.0	41.0
41	36.45601392642693	39.0	35.0	40.0	31.0	41.0
42	36.570083145828065	39.0	35.0	41.0	31.0	41.0
43	36.51135017108469	39.0	35.0	41.0	31.0	41.0
44	36.51970136734612	39.0	35.0	41.0	31.0	41.0
45	36.542980867805454	39.0	35.0	41.0	31.0	41.0
46	36.39484449267065	38.0	35.0	40.0	31.0	41.0
47	36.29156126429589	38.0	35.0	40.0	31.0	41.0
48	36.257515743785696	38.0	35.0	40.0	31.0	41.0
49	36.28046405890106	38.0	35.0	40.0	31.0	41.0
50	36.209953201347375	38.0	35.0	40.0	31.0	41.0
51	35.331008933683044	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	22.0
9	45.0
10	47.0
11	54.0
12	51.0
13	44.0
14	51.0
15	72.0
16	88.0
17	155.0
18	221.0
19	371.0
20	636.0
21	1126.0
22	1533.0
23	2095.0
24	2930.0
25	3720.0
26	4716.0
27	5483.0
28	6213.0
29	7354.0
30	9299.0
31	12278.0
32	16699.0
33	25532.0
34	47874.0
35	50194.0
36	51443.0
37	79558.0
38	134061.0
39	136889.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67685630217417	22.559213942403705	21.748725186062924	12.015204569359197
2	32.12830686069579	25.70064839100507	24.099475429043125	18.071569319256014
3	33.03898334420642	25.118328029929838	25.18356654994741	16.659122075916336
4	29.358332556684285	24.94008707345325	26.244358199416844	19.45722217044562
5	26.786570184664953	30.47354511443369	22.99258411109188	19.747300589809477
6	26.72715653250609	34.36738606558468	24.07800663036387	14.827450771545353
7	77.27502696081694	6.875507595627687	10.76086088218456	5.088604561370808
8	77.65347694683726	7.65154641920409	9.356568453847075	5.338408180111571
9	71.30453740563713	8.194257678840087	11.639750229666218	8.861454685856556
10	43.5220812419284	23.446091680091598	18.196554341024378	14.835272736955623
11	33.52094955331585	24.479090388635182	22.9915855623161	19.00837449573287
12	30.497843134644313	20.919430427778295	27.654309070817078	20.928417366760307
13	26.552909771132622	23.33641773955185	28.17821432850923	21.932458160806295
14	21.366114580143524	27.168681516196465	29.06925268609621	22.395951217563805
15	21.703124791969007	25.01231543490128	32.38293679851949	20.901622974610234
16	25.22434062495839	25.172915363005764	29.299917453301198	20.302826558734637
17	23.78043909518167	25.92116124565631	27.66412813377891	22.63427152538311
18	25.089037265840314	26.104394946011794	27.43179911861428	21.374768669533612
19	25.099189178394067	28.25393761067249	25.777536646740074	20.86933656419337
20	27.859843693831632	26.43524744038664	27.264209349079337	18.44069951670239
21	26.191102264708622	28.195522507289404	26.748958180777272	18.8644170472247
22	24.31516196461143	24.34228920635343	28.381086154788377	22.96146267424676
23	25.338008760601262	25.295736862426605	27.727535981040884	21.638718395931246
24	25.633246348639975	24.139417380074292	28.14276584696907	22.08457042431666
25	22.877418152285344	26.15482165918865	26.909890958473685	24.057869230052322
26	21.841423797414425	26.57321359624013	28.689637726504145	22.895724879841296
27	22.85628220319802	27.06965876259836	27.424809277183826	22.6492497570198
28	20.095627687760455	29.098876299777654	28.737900917333477	22.067595095128414
29	23.651792727902116	27.778295543809662	27.154868258131515	21.415043470156707
30	23.66660453474284	27.12025190057117	29.02348586720633	20.189657697479664
31	25.91417140422586	26.263996325340504	26.366181150061912	21.455651120371726
32	26.439907334673606	28.18021142606079	26.09840365335712	19.28147758590848
33	26.143837622655074	26.465703178047907	25.74674805948688	21.643711139810144
34	23.07230158835825	27.41732016136548	28.57114327177835	20.939234978497918
35	23.69140182934136	29.102204795696924	26.078266253045573	21.128127121916148
36	24.751028505239052	30.87080110239785	24.937257851921874	19.440912540441225
37	25.16243060086008	28.965570038211137	25.908180111571184	19.963819249357602
38	23.831198657950445	28.655687068127655	25.290577693751747	22.222536580170154
39	26.29445206300177	25.903353792488247	25.73642972213716	22.06576442237282
40	25.90751441238733	24.359597385133604	27.338934082466814	22.39395412001225
41	22.3674925774541	26.815694523958513	26.640282789013302	24.176530109574085
42	23.95202305981973	25.77320960204503	26.913385879188912	23.361381458946333
43	24.195336111517925	25.229666218429216	26.604335033085245	23.970662636967607
44	22.963293347002356	25.87622655074625	27.30015710500739	23.860322997244005
45	24.213476414277917	25.02146879867925	26.550080549601248	24.214974237441584
46	24.321486106858035	27.324288700422056	26.152158862453234	22.20206633026668
47	22.552889800157104	25.94279646913153	29.086560864876382	22.417752865834988
48	23.13770653317179	25.305555925388433	29.01266825546872	22.544069285971055
49	24.502223435274068	23.738000772211056	29.227522667057208	22.532253125457668
50	22.067595095128414	24.604408259995473	28.57513746688147	24.752859177994647
51	21.595780798572743	23.42062868630923	28.425521575310547	26.558068939807477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	304.0
1	719.5
2	1135.0
3	2339.5
4	3544.0
5	2476.5
6	1409.0
7	1361.5
8	1314.0
9	1261.5
10	1209.0
11	1177.0
12	1145.0
13	1227.5
14	1310.0
15	1244.0
16	1178.0
17	1249.0
18	1320.0
19	1357.0
20	1394.0
21	1675.5
22	1957.0
23	2085.5
24	2214.0
25	2910.0
26	4227.0
27	4848.0
28	5602.0
29	6356.0
30	7774.0
31	9192.0
32	10957.5
33	12723.0
34	14392.0
35	16061.0
36	16312.5
37	16564.0
38	18868.0
39	21172.0
40	22915.0
41	24658.0
42	27517.5
43	30377.0
44	33057.5
45	35738.0
46	44208.0
47	52678.0
48	52737.0
49	52796.0
50	52910.5
51	53025.0
52	47538.5
53	42052.0
54	39372.5
55	36693.0
56	34651.5
57	32610.0
58	31231.0
59	29852.0
60	29681.0
61	29510.0
62	26332.0
63	23154.0
64	19537.0
65	15920.0
66	13294.5
67	10669.0
68	8968.5
69	7268.0
70	6074.0
71	4880.0
72	4264.0
73	3648.0
74	3234.0
75	1929.0
76	1038.0
77	953.0
78	868.0
79	595.0
80	322.0
81	233.0
82	144.0
83	118.0
84	92.0
85	92.5
86	93.0
87	50.0
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	600872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.588409711683298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74623400963962	21.082875525612504
2	9.040867589483277	5.16928053594453
3	3.048287495705315	2.614370755386738
4	1.619719394800062	1.8522080670601557
5	1.0890427974406365	1.556700084339532
6	0.8386346549107897	1.4385138667803476
7	0.7225337281186689	1.4459263174976544
8	0.6343562322171463	1.4508188695826791
9	0.6109392994113308	1.5719204700485891
>10	8.376848692804455	52.636044061102496
>50	0.20292731662605873	3.6326015715941358
>100	0.06429964393093138	3.1907530981690355
>500	0.0017697149705760927	0.34319146695422764
>1k	0.0035394299411521855	2.0147953099273503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC	2470	0.4110692460291044	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG	2414	0.40174945745516516	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC	2170	0.36114180724014433	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	1964	0.32685829927172505	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1733	0.28841417140422587	No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	1196	0.19904405597198738	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	920	0.15311081228614412	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15943495453274575	0.0
2	0.0	0.0	0.0	0.8402787948181976	0.0
3	0.0	0.0	0.0	1.107557017135097	0.0
4	0.0	0.0	0.0	1.5164627408166798	0.0
5	0.0	0.0	0.0	2.7653144097245335	0.0
6	1.6642479596320014E-4	0.0	0.0	3.313517687627315	0.0
7	1.6642479596320014E-4	0.0	0.0	3.897335871866221	0.0
8	1.6642479596320014E-4	0.0	0.0	4.645248904924842	0.0
9	1.6642479596320014E-4	0.0	0.0	4.945146387250529	0.0
10	1.6642479596320014E-4	0.0	0.0	5.973485201507143	0.0
11	1.6642479596320014E-4	0.0	0.0	6.97236682687827	0.0
12	1.6642479596320014E-4	0.0	0.0	8.10705108575537	0.0
13	1.6642479596320014E-4	0.0	0.0	8.481174027080643	0.0
14	1.6642479596320014E-4	0.0	0.0	8.648597371819623	0.0
15	1.6642479596320014E-4	0.0	0.0	8.987937530788587	0.0
16	1.6642479596320014E-4	0.0	0.0	9.549787641960352	0.0
17	1.6642479596320014E-4	0.0	0.0	10.165392962228228	1.6642479596320014E-4
18	1.6642479596320014E-4	0.0	0.0	10.859051511802846	1.6642479596320014E-4
19	3.328495919264003E-4	0.0	0.0	11.393275106844719	1.6642479596320014E-4
20	3.328495919264003E-4	0.0	0.0	11.84628340145655	1.6642479596320014E-4
21	3.328495919264003E-4	0.0	0.0	12.367026588025404	1.6642479596320014E-4
22	3.328495919264003E-4	0.0	0.0	12.878449986020318	1.6642479596320014E-4
23	3.328495919264003E-4	0.0	0.0	13.390372658403122	1.6642479596320014E-4
24	3.328495919264003E-4	0.0	0.0	13.834394014032938	1.6642479596320014E-4
25	3.328495919264003E-4	0.0	0.0	14.231317152405172	1.6642479596320014E-4
26	3.328495919264003E-4	0.0	0.0	14.57548363045707	1.6642479596320014E-4
27	3.328495919264003E-4	0.0	0.0	14.928304197899052	1.6642479596320014E-4
28	3.328495919264003E-4	0.0	0.0	15.301595015244512	1.6642479596320014E-4
29	3.328495919264003E-4	0.0	0.0	15.653749883502643	1.6642479596320014E-4
30	4.992743878896004E-4	0.0	0.0	16.059659960856887	1.6642479596320014E-4
31	4.992743878896004E-4	0.0	0.0	16.44360196514399	1.6642479596320014E-4
32	4.992743878896004E-4	0.0	0.0	16.839193705148517	1.6642479596320014E-4
33	4.992743878896004E-4	0.0	0.0	17.219307939128466	1.6642479596320014E-4
34	4.992743878896004E-4	0.0	0.0	17.589103835758696	1.6642479596320014E-4
35	4.992743878896004E-4	0.0	0.0	17.988356921274413	1.6642479596320014E-4
36	4.992743878896004E-4	0.0	0.0	18.352827224433824	1.6642479596320014E-4
37	4.992743878896004E-4	0.0	0.0	18.744591194131196	1.6642479596320014E-4
38	4.992743878896004E-4	0.0	0.0	19.20725212690889	1.6642479596320014E-4
39	4.992743878896004E-4	0.0	0.0	19.644283641108256	1.6642479596320014E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	30	2.163766E-6	45.000004	31
AACCGTC	20	7.0305966E-4	45.0	39
CGACGTT	25	3.8885755E-5	45.0	27
TTAGCCG	20	7.0305966E-4	45.0	1
AACCCGA	25	3.8885755E-5	45.0	24
TCGATTG	20	7.0305966E-4	45.0	1
CGTTCTA	20	7.0305966E-4	45.0	23
TACGAAT	90	0.0	45.0	12
AGTTACG	25	3.8885755E-5	45.0	1
TATAGCG	25	3.8885755E-5	45.0	1
ATAACGG	45	3.8380676E-10	45.0	2
CACGTCA	25	3.8885755E-5	45.0	34
TCAACCG	20	7.0305966E-4	45.0	37
ACGAATG	20	7.0305966E-4	45.0	1
ACACGAC	125	0.0	43.2	26
AAACACG	120	0.0	43.125004	40
CGTTTTT	930	0.0	40.64516	1
CGAATAT	100	0.0	40.5	14
AACGACG	50	1.0804797E-9	40.5	13
CACGACC	135	0.0	40.000004	27
>>END_MODULE