##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692613
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334505705206226	31.0	31.0	33.0	30.0	34.0
2	31.31668334264589	31.0	31.0	34.0	28.0	34.0
3	31.224197351190348	31.0	31.0	34.0	28.0	34.0
4	35.060618267344104	35.0	35.0	37.0	32.0	37.0
5	35.277200976591544	35.0	35.0	37.0	33.0	37.0
6	35.184316494203834	36.0	35.0	37.0	33.0	37.0
7	35.50016098456136	37.0	35.0	37.0	33.0	37.0
8	35.02792179759837	37.0	35.0	37.0	32.0	37.0
9	36.84209363670621	39.0	37.0	39.0	32.0	39.0
10	36.460258470458975	38.0	35.0	39.0	32.0	39.0
11	36.675537421330525	38.0	35.0	39.0	32.0	39.0
12	36.68695794043715	39.0	35.0	39.0	33.0	39.0
13	36.61223078400203	39.0	35.0	39.0	32.0	39.0
14	37.63479605493977	39.0	36.0	41.0	33.0	41.0
15	37.63593521923499	40.0	36.0	41.0	32.0	41.0
16	37.8588721262812	40.0	36.0	41.0	33.0	41.0
17	37.55090360706484	39.0	36.0	41.0	32.0	41.0
18	37.58565750281903	39.0	36.0	41.0	33.0	41.0
19	37.49713909499244	39.0	36.0	41.0	33.0	41.0
20	37.18702363368865	39.0	35.0	41.0	32.0	41.0
21	37.236212719079774	39.0	35.0	40.0	32.0	41.0
22	37.353614500449744	39.0	35.0	41.0	32.0	41.0
23	37.31834083391447	39.0	35.0	41.0	32.0	41.0
24	37.289015655207166	39.0	35.0	41.0	32.0	41.0
25	37.062752215162	39.0	35.0	40.0	32.0	41.0
26	37.02862060053739	39.0	35.0	41.0	32.0	41.0
27	37.04860578707012	39.0	35.0	41.0	32.0	41.0
28	36.92118686770245	39.0	35.0	41.0	31.0	41.0
29	36.93765782623196	39.0	35.0	41.0	31.0	41.0
30	36.66344841924711	39.0	35.0	41.0	31.0	41.0
31	36.56499805807861	39.0	35.0	40.0	30.0	41.0
32	36.329855200523234	39.0	35.0	40.0	30.0	41.0
33	36.308662990732195	39.0	35.0	41.0	30.0	41.0
34	36.04663354571745	39.0	35.0	41.0	29.0	41.0
35	35.986885894431666	39.0	35.0	41.0	29.0	41.0
36	35.880783352319405	39.0	35.0	41.0	27.0	41.0
37	35.68728424098306	39.0	35.0	41.0	27.0	41.0
38	35.53339166316543	39.0	35.0	41.0	26.0	41.0
39	35.54556007467374	39.0	35.0	40.0	26.0	41.0
40	35.39802169465488	38.0	35.0	40.0	25.0	41.0
41	35.35828088701771	38.0	35.0	40.0	25.0	41.0
42	35.294027111821464	38.0	35.0	40.0	25.0	41.0
43	35.32994904802538	38.0	35.0	40.0	26.0	41.0
44	35.5287902479451	39.0	35.0	40.0	26.0	41.0
45	35.4590528910084	39.0	35.0	40.0	26.0	41.0
46	35.436561254264646	38.0	35.0	40.0	26.0	41.0
47	35.10769650584093	38.0	35.0	40.0	24.0	41.0
48	35.146459855648104	38.0	35.0	40.0	25.0	41.0
49	35.259807424925604	38.0	35.0	40.0	26.0	41.0
50	35.11790278265063	38.0	35.0	40.0	26.0	41.0
51	34.345116248178996	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	12.0
9	24.0
10	30.0
11	30.0
12	24.0
13	21.0
14	26.0
15	37.0
16	74.0
17	143.0
18	273.0
19	636.0
20	1830.0
21	3692.0
22	4988.0
23	5828.0
24	6925.0
25	8150.0
26	9178.0
27	9700.0
28	9905.0
29	10385.0
30	12430.0
31	15486.0
32	20305.0
33	28782.0
34	49379.0
35	51367.0
36	53676.0
37	81960.0
38	144456.0
39	162833.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.09381285075504	15.471699202873754	18.19024476872366	18.244243177647547
2	28.845545780977254	33.227502227073415	22.121011300683065	15.805940691266262
3	28.448498656536913	32.39023812720812	25.30431857328696	13.856944642968006
4	25.978576780972922	21.383658695404215	35.931032192580844	16.70673233104201
5	31.567267723822685	24.308091242873004	25.163980462393866	18.960660570910452
6	23.743851183850147	37.32170779352972	25.441913449502103	13.492527573118032
7	68.76365300680179	5.785481935799646	20.887999503330143	4.562865554068433
8	68.60844367633874	13.119736418461681	13.017370450742332	5.254449454457251
9	63.07187419237006	6.682519675489776	14.00017036931158	16.245435762828592
10	43.32448279197762	19.912563004159612	21.5427663067254	15.220187897137363
11	33.8526709721013	22.208072906514893	26.147213523280676	17.792042598103126
12	27.150371130775774	19.98244329806111	29.383219777855746	23.483965793307373
13	27.519408385346505	20.093472112131884	33.78538953210523	18.60172997041638
14	20.662476736648028	28.00496092334392	30.276359236687732	21.056203103320325
15	18.9694677980344	23.4458492693611	36.29848125865382	21.28620167395068
16	22.7993121699997	25.178562920418763	27.52417295083979	24.49795195874175
17	22.296145177754386	28.122198110633214	30.16648546879715	19.41517124281525
18	21.987747847643632	25.416502433537918	29.223534643444466	23.37221507537398
19	21.385246883901978	27.11846298004802	27.51421067753565	23.982079458514352
20	26.14779104637077	26.499502608238657	31.48164992571609	15.871056419674478
21	23.077100776335413	30.637311167997133	29.38740682025893	16.89818123540852
22	21.254293523222927	21.615389835304853	34.976964047743834	22.153352593728386
23	24.641755208175418	25.39614474461207	31.60408482081624	18.358015226396272
24	23.90411384135152	25.31269265809334	28.696544823732733	22.08664867682241
25	20.807001889944313	31.802319621491364	26.54859207089674	20.842086417667584
26	20.2576330504914	24.600606688006145	31.882595331014578	23.259164930487877
27	25.295800107708054	25.469923319371713	27.526194281655126	21.708082291265107
28	18.463701951883664	29.843938822979066	29.94688231378851	21.745476911348764
29	23.110452734788403	27.573406794270394	29.15018921100239	20.16595125993881
30	24.26030120716764	29.207363996921803	28.392478916797693	18.13985587911287
31	23.7666633459089	26.67882352771317	25.372177536373126	24.182335590004808
32	27.910824659658424	29.803223445127365	24.566677206463062	17.71927468875115
33	28.130283433894544	24.585302326118626	24.670342601135122	22.61407163885171
34	22.432585007789342	27.218807616952034	26.720838332517584	23.62776904274104
35	25.25450720676626	28.431750486924155	25.391813321436356	20.921928984873226
36	23.8304796473644	33.81166683270456	23.73403329131853	18.623820228612516
37	24.25423721472164	28.78317328724699	26.57342556377082	20.389163934260544
38	23.981935077741827	29.008984815474154	23.492773020431322	23.516307086352697
39	24.03189082503505	28.047553251238426	25.181594916641757	22.738961007084765
40	24.101915499709072	25.89627974063438	26.963975553447593	23.03782920620895
41	21.711547429805677	27.01941777009672	25.27529803800968	25.99373676208792
42	21.56500094569406	27.17376081592462	29.55936432033473	21.701873918046584
43	25.489414723662417	25.396866648474692	25.916059906470135	23.197658721392756
44	23.644805973898844	23.45624468498281	28.272354114057922	24.62659522706042
45	21.959016073911407	23.398492375973305	27.42483897934344	27.217652570771843
46	25.380551621179503	27.648629176755275	25.607806957131906	21.36301224493332
47	19.92295841978132	26.316139027133477	32.63684048667871	21.124062066406495
48	21.353627494719273	26.759243618008906	27.727605459325773	24.159523427946052
49	22.083327919054362	23.735043956726194	32.04747817323671	22.134149950982728
50	22.32126743217352	24.185945109317903	29.408486413047402	24.084301045461174
51	20.502647221464223	23.66963946677293	28.248964428909073	27.57874888285377
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	521.5
2	932.0
3	5561.0
4	10190.0
5	6788.5
6	3387.0
7	3248.5
8	3110.0
9	3067.0
10	3024.0
11	2937.0
12	2850.0
13	2699.5
14	2549.0
15	2536.5
16	2524.0
17	2508.5
18	2493.0
19	2408.0
20	2323.0
21	2364.0
22	2405.0
23	2648.0
24	2891.0
25	3330.0
26	4679.5
27	5590.0
28	6388.5
29	7187.0
30	8409.5
31	9632.0
32	11143.5
33	12655.0
34	14850.0
35	17045.0
36	18129.5
37	19214.0
38	20481.0
39	21748.0
40	23607.5
41	25467.0
42	27971.0
43	30475.0
44	33430.0
45	36385.0
46	67769.0
47	99153.0
48	78030.5
49	56908.0
50	57418.0
51	57928.0
52	52931.5
53	47935.0
54	44905.5
55	41876.0
56	39495.0
57	37114.0
58	34355.0
59	31596.0
60	29985.5
61	28375.0
62	25369.5
63	22364.0
64	19028.0
65	15692.0
66	12820.0
67	9948.0
68	7851.0
69	5754.0
70	4913.0
71	4072.0
72	3285.5
73	2499.0
74	2086.0
75	1287.5
76	902.0
77	701.5
78	501.0
79	360.0
80	219.0
81	154.0
82	89.0
83	62.5
84	36.0
85	23.5
86	11.0
87	8.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	692613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.41986153781582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57797656642005	24.63704042573204
2	8.207817958878728	5.650239153444078
3	3.5772550801891616	3.693858736366775
4	2.458017624129179	3.3841850512014946
5	1.998956688294539	3.4401906215594447
6	1.7014114547119745	3.513740801402397
7	1.4910650760435535	3.5925577422904356
8	1.2966249798967813	3.570372181961635
9	1.162853646733417	3.6022735358377562
>10	6.449700347764072	31.92958876753239
>50	0.04529892833094752	1.0896793717825206
>100	0.026247977098392817	1.6668684554558444
>500	0.002116772346644582	0.46542336276999446
>1k	0.0029634812853024145	2.8253267316053297
>5k	4.233544693289163E-4	0.7686625668790611
>10k+	0.001270063407986749	6.169992494178806
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	16379	2.3648126731666888	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	13178	1.9026498203181288	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	12785	1.8459081767162906	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5275	0.7616085750628417	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	4297	0.620404179534603	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCT	4138	0.5974476367033249	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCT	2657	0.3836197125956342	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAAGCAGT	2651	0.38275342796049167	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTC	1987	0.286884595004714	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTA	1965	0.28370821800919127	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTT	1694	0.24458102865525191	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	832	0.12012480273976955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4438077252376146E-4	0.0	0.0	0.12719946059343384	0.0
2	1.4438077252376146E-4	0.0	0.0	0.9273577019201199	0.0
3	1.4438077252376146E-4	0.0	0.0	1.1346884912642414	0.0
4	1.4438077252376146E-4	0.0	0.0	1.970797544949344	0.0
5	1.4438077252376146E-4	0.0	0.0	4.992542733099148	0.0
6	1.4438077252376146E-4	0.0	0.0	5.949642874159163	0.0
7	1.4438077252376146E-4	0.0	0.0	6.818237601662111	0.0
8	1.4438077252376146E-4	0.0	0.0	7.689286802297964	0.0
9	1.4438077252376146E-4	0.0	0.0	8.038110748715372	0.0
10	1.4438077252376146E-4	0.0	0.0	10.510487097412263	0.0
11	1.4438077252376146E-4	0.0	0.0	11.301838111615	0.0
12	1.4438077252376146E-4	0.0	0.0	13.78605368365884	0.0
13	1.4438077252376146E-4	0.0	0.0	14.12578164140725	0.0
14	1.4438077252376146E-4	0.0	0.0	14.327914722940516	0.0
15	1.4438077252376146E-4	0.0	0.0	14.806392603084262	0.0
16	1.4438077252376146E-4	0.0	0.0	15.2040172506147	0.0
17	1.4438077252376146E-4	0.0	0.0	15.60164189814514	0.0
18	1.4438077252376146E-4	0.0	0.0	15.963748875634733	0.0
19	1.4438077252376146E-4	0.0	0.0	16.704855380999202	0.0
20	1.4438077252376146E-4	0.0	0.0	17.071582543209555	0.0
21	1.4438077252376146E-4	0.0	0.0	17.41982896653687	0.0
22	1.4438077252376146E-4	0.0	0.0	17.854270711060867	0.0
23	1.4438077252376146E-4	0.0	0.0	18.2002070420278	0.0
24	1.4438077252376146E-4	0.0	0.0	18.492578106388414	0.0
25	1.4438077252376146E-4	0.0	0.0	18.76459148182318	0.0
26	1.4438077252376146E-4	0.0	0.0	19.029097057086712	0.0
27	1.4438077252376146E-4	0.0	0.0	19.336194960244754	0.0
28	1.4438077252376146E-4	0.0	0.0	19.611384712675044	0.0
29	1.4438077252376146E-4	0.0	0.0	19.89480416913919	0.0
30	1.4438077252376146E-4	0.0	0.0	20.2369866000205	0.0
31	1.4438077252376146E-4	0.0	0.0	20.530801472106358	0.0
32	1.4438077252376146E-4	0.0	0.0	20.84223079844011	0.0
33	1.4438077252376146E-4	0.0	0.0	21.132291770440347	0.0
34	1.4438077252376146E-4	0.0	0.0	21.441122242868673	0.0
35	1.4438077252376146E-4	0.0	0.0	21.747065099846523	0.0
36	1.4438077252376146E-4	0.0	0.0	22.067157272531702	0.0
37	1.4438077252376146E-4	0.0	0.0	22.39981057242645	0.0
38	1.4438077252376146E-4	0.0	0.0	22.82400128210126	0.0
39	1.4438077252376146E-4	0.0	0.0	23.346082155547183	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	20	7.031342E-4	45.000004	1
GCGCATA	20	7.031342E-4	45.000004	1
GCGATAT	20	7.031342E-4	45.000004	9
TTAACGG	30	2.1642481E-6	45.000004	2
CAATCGA	40	6.8084773E-9	45.000004	41
CGATCGA	40	6.8084773E-9	45.000004	41
TCGACAC	30	2.1642481E-6	45.000004	34
ACCGCGA	25	3.8891954E-5	45.0	30
CCGTCGA	100	0.0	45.0	41
ACGCGTA	25	3.8891954E-5	45.0	40
TACGGCT	1250	0.0	42.84	7
CGTTTTT	3220	0.0	42.6941	1
GTCGAAT	260	0.0	42.403843	43
CGATGAA	2115	0.0	41.595745	19
GATACCT	1485	0.0	41.515152	5
CGGCGAA	190	0.0	41.44737	31
ACGGCTG	1310	0.0	41.39313	8
CCGATGA	2100	0.0	41.14286	18
CGAATGC	350	0.0	41.142857	45
CCCGTCG	115	0.0	41.08696	40
>>END_MODULE