FastQCFastQC Report
Sat 18 Jun 2016
SRR3551736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323079
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGC113983.5279297014043003No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC110153.409382844443619No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCG100333.105432417458269No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC33951.0508265780196173No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30030.9294940246812698No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCT23450.7258286672919007No Hit
GAACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT23100.7149954036009768No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAACCGAT21000.6499958214554336No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTA19850.614400812185255No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTC18680.5781867592755952No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC7280.22533188477121693No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT7030.2175938392776999Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATG6850.2120224465223676No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTT6680.206760575586776No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC5520.17085604449685682No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAACCGATCGT4840.1498085607544904No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCAACCGA4540.14052290616226992No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTAT4340.13433246976745625No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC3650.11297546420534915No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.0257485E-445.0000046
ACCGAAC351.2083001E-745.00000442
CGACAGG406.7884685E-945.0000042
ACGGACG207.0257485E-445.00000443
GACGTAG253.8845596E-545.01
TAGTTGG253.8845596E-545.02
CACGTGA253.8845596E-545.043
TCGAATA253.8845596E-545.044
AACACGT253.8845596E-545.041
ATTAGTC302.1606393E-644.99999631
TACGGCT12550.044.462157
CGATGAA8450.043.13609319
GATACCT10650.042.887325
ACGGCTG13050.042.7586178
CGTTTTT17050.042.75661
ATACCTG10950.041.917816
ATGAATG9100.041.78571321
CGGCTGT13300.041.7857139
TGATACC11000.041.7272724
GATGAAT9150.041.55737720
Produced by FastQC (version 0.11.5)