##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323079
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26431306274936	31.0	31.0	33.0	28.0	34.0
2	31.198691341746137	31.0	31.0	34.0	28.0	34.0
3	31.10829549429087	31.0	31.0	34.0	28.0	34.0
4	34.97539920576701	35.0	35.0	37.0	32.0	37.0
5	35.23674395426506	35.0	35.0	37.0	33.0	37.0
6	35.16249895536386	36.0	35.0	37.0	33.0	37.0
7	35.40898975173255	36.0	35.0	37.0	33.0	37.0
8	34.735850983815105	36.0	35.0	37.0	32.0	37.0
9	36.539403675262086	39.0	35.0	39.0	32.0	39.0
10	36.33116977581334	37.0	35.0	39.0	32.0	39.0
11	36.694480297388566	38.0	35.0	39.0	32.0	39.0
12	36.77480740004766	39.0	35.0	39.0	33.0	39.0
13	36.674513044797095	39.0	35.0	39.0	32.0	39.0
14	37.75601633037121	39.0	37.0	41.0	33.0	41.0
15	37.64912916036016	40.0	36.0	41.0	32.0	41.0
16	37.93623540991522	39.0	37.0	41.0	33.0	41.0
17	37.58013674673996	39.0	36.0	41.0	32.0	41.0
18	37.5506083651367	39.0	36.0	40.0	32.0	41.0
19	37.49112136660074	39.0	36.0	40.0	32.0	41.0
20	37.13370723569158	39.0	35.0	40.0	31.0	41.0
21	37.26216807653856	39.0	35.0	40.0	32.0	41.0
22	37.35590985486522	39.0	35.0	41.0	32.0	41.0
23	37.29712547086007	39.0	35.0	41.0	32.0	41.0
24	37.165925361908386	39.0	35.0	41.0	32.0	41.0
25	37.00900089451806	39.0	35.0	40.0	32.0	41.0
26	37.028828862290645	39.0	35.0	40.0	31.0	41.0
27	36.98309082298756	39.0	35.0	41.0	31.0	41.0
28	36.80587100987684	39.0	35.0	41.0	31.0	41.0
29	36.945771777181434	39.0	35.0	41.0	31.0	41.0
30	36.62900714685882	39.0	35.0	41.0	30.0	41.0
31	36.544758402743604	39.0	35.0	40.0	30.0	41.0
32	36.24775674061143	39.0	35.0	40.0	30.0	41.0
33	36.044933282571755	39.0	35.0	41.0	28.0	41.0
34	35.922650497246806	39.0	35.0	41.0	27.0	41.0
35	35.815664280253436	39.0	35.0	41.0	27.0	41.0
36	35.703168574868684	39.0	35.0	41.0	26.0	41.0
37	35.70093382733016	39.0	35.0	41.0	26.0	41.0
38	35.38453443275484	39.0	35.0	40.0	24.0	41.0
39	35.37035214297432	38.0	35.0	40.0	25.0	41.0
40	35.34467730802683	38.0	35.0	40.0	25.0	41.0
41	35.09774699067411	38.0	34.0	40.0	24.0	41.0
42	35.16456346590153	38.0	35.0	40.0	24.0	41.0
43	35.20843199341338	38.0	35.0	40.0	24.0	41.0
44	35.40014361812436	39.0	35.0	40.0	25.0	41.0
45	35.306547315052974	39.0	35.0	40.0	24.0	41.0
46	35.2399134576992	38.0	35.0	40.0	24.0	41.0
47	34.71710943762981	38.0	34.0	40.0	23.0	41.0
48	34.809433606022054	38.0	34.0	40.0	23.0	41.0
49	35.01051755143479	38.0	34.0	40.0	24.0	41.0
50	34.92376168057967	38.0	34.0	40.0	24.0	41.0
51	33.993082187328795	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	19.0
11	9.0
12	10.0
13	17.0
14	18.0
15	15.0
16	28.0
17	61.0
18	115.0
19	237.0
20	532.0
21	1207.0
22	2085.0
23	2928.0
24	3672.0
25	4301.0
26	5019.0
27	5025.0
28	4899.0
29	5142.0
30	6249.0
31	7845.0
32	9802.0
33	13299.0
34	20871.0
35	24055.0
36	27384.0
37	40915.0
38	69705.0
39	67595.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.76776887386676	14.797000114523073	15.605471107685737	18.82975990392443
2	27.112563800185097	37.92570857282584	18.992258859288285	15.969468767700778
3	24.918673141863135	37.048523735680746	25.04433900067785	12.988464121778264
4	23.91767957682177	19.77968236870858	40.962736668121416	15.339901386348231
5	34.163780375697584	22.270404452161856	24.705412608061806	18.860402564078754
6	21.079054967979967	39.93667183568106	25.913166748689946	13.071106447649028
7	62.39588459788473	5.2869422029906	26.740828094676534	5.576345104448138
8	59.511450759721306	17.514911213666007	15.901064445538088	7.072573581074598
9	54.93424208939609	7.093621064816964	15.608256804063403	22.36388004172354
10	39.05391560578063	21.96893019973443	23.218779307847306	15.758374886637633
11	32.840884118125906	20.577320098180323	27.740893094258677	18.84090268943509
12	24.982434636729717	18.199263957112656	30.980038937844924	25.838262468312706
13	25.66462072743818	19.943419411351403	37.310069673361625	17.081890187848792
14	19.687754388245597	29.829546333868805	27.57777509525534	22.904924182630253
15	15.592780713076367	22.664425728691747	39.93512422658235	21.807669331649535
16	17.591672624961696	25.02979147515004	28.48560259255476	28.8929333073335
17	17.967122592307145	27.50379938033732	32.799098672460914	21.729979354894624
18	17.444340238765133	25.126671804728872	31.21527552084784	26.213712435658152
19	18.403238836321766	25.588478359781973	28.67843468625321	27.329848117643053
20	23.308231113752363	24.97841085307309	35.009703509048876	16.703654524125678
21	21.114959499069887	31.923770966234265	29.289121236601574	17.672148298094275
22	18.79168872009632	21.240625357884603	33.86602038510705	26.101665536912023
23	21.631241894397345	28.357151037362378	31.12984749859941	18.881759569640863
24	22.326737423354658	24.405176442913344	27.71798847959787	25.55009765413413
25	17.56226805208633	34.752181355024625	26.136331980722982	21.549218612166065
26	18.26859684473457	22.891924266201148	33.673497813228344	25.16598107583594
27	24.4398428867243	26.02490412561633	26.391377960189306	23.143875027470063
28	15.830802992456952	27.951677453502082	33.65956933134001	22.55795022270095
29	20.2424174892209	22.43135579842701	33.29495262768549	24.0312740846666
30	17.473435289820756	28.732601004707824	29.35969221150245	24.434271493968968
31	21.106911931756628	26.645185852376663	24.459342761367964	27.788559454498746
32	25.004101164111564	31.029871950823175	24.047988262932595	19.918038622132666
33	20.278941063950302	24.55003265455198	23.096208667229998	32.07481761426772
34	22.031763129141787	23.70070478118355	27.733464570584903	26.53406751908976
35	24.030964562846858	23.3716830867992	23.762609145131684	28.834743205222253
36	18.27293015021094	32.95509766961022	26.43502053677274	22.336951643406103
37	18.412524490913988	27.56384661336701	28.472602676125653	25.551026219593346
38	19.34511373379266	29.015503947950812	22.688877952451257	28.95050436580527
39	21.006317340340907	28.966289978612043	25.719715611352022	24.30767706969503
40	22.750163272759913	21.272506105317895	28.502007249001014	27.475323372921174
41	18.457095632956648	28.605697058614147	24.502675816131656	28.43453149229755
42	24.335224511651948	23.766632928788315	30.202520126656328	21.69562243290341
43	22.437546234821824	28.58031626939541	24.379176610055126	24.60296088572764
44	21.37991017676791	22.765329841927205	28.826386116089253	27.028373865215627
45	20.329393120568035	21.518575952011737	26.745780443792384	31.406250483627847
46	26.639923981441072	27.348109905007757	24.852435472438632	21.159530641112546
47	17.08127114420931	23.688323908393922	36.54028890766654	22.69011603973022
48	20.71722396070311	25.20714747786145	26.3700209546272	27.70560760680824
49	20.40986879370061	20.945960585491473	35.055512738370496	23.58865788243742
50	21.463171546278154	22.215309568248014	30.206853432132696	26.114665453341136
51	19.521541171044852	20.752199926333805	27.39608578706756	32.33017311555378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48.0
1	241.5
2	435.0
3	2804.0
4	5173.0
5	3557.5
6	1942.0
7	1832.5
8	1723.0
9	1679.0
10	1635.0
11	1555.5
12	1476.0
13	1471.5
14	1467.0
15	1381.5
16	1296.0
17	1265.5
18	1235.0
19	1203.5
20	1172.0
21	1209.5
22	1247.0
23	1219.0
24	1191.0
25	1342.5
26	1830.0
27	2166.0
28	2629.0
29	3092.0
30	3449.5
31	3807.0
32	4317.0
33	4827.0
34	5481.0
35	6135.0
36	6772.5
37	7410.0
38	8022.5
39	8635.0
40	9879.5
41	11124.0
42	11922.5
43	12721.0
44	14753.5
45	16786.0
46	40211.0
47	63636.0
48	46992.5
49	30349.0
50	30015.5
51	29682.0
52	25776.5
53	21871.0
54	19658.5
55	17446.0
56	15568.5
57	13691.0
58	12484.0
59	11277.0
60	10691.0
61	10105.0
62	9016.0
63	7927.0
64	6844.5
65	5762.0
66	4831.5
67	3901.0
68	3345.5
69	2790.0
70	2347.0
71	1904.0
72	1717.5
73	1531.0
74	1296.5
75	983.5
76	905.0
77	701.5
78	498.0
79	362.5
80	227.0
81	191.0
82	155.0
83	111.0
84	67.0
85	56.5
86	46.0
87	25.0
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.51692804703882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29596833678661	27.87356498276298
2	9.110767823943148	6.836160418083029
3	3.6764323796715055	4.137853471738187
4	2.329256712465044	3.49546225938533
5	1.7552140999058872	3.292512054665859
6	1.5070933876698334	3.3924908511226275
7	1.2191149365461622	3.2016213148830865
8	1.028035756524065	3.0854994805837173
9	0.8915661611447809	3.010394116515903
>10	4.102190841869924	22.361032994586147
>50	0.04962327263120628	1.253553250834128
>100	0.022330472684042826	1.680506041217237
>500	0.00413527271926719	1.0351122883125374
>1k	0.005789381806974065	5.276714500912173
>5k	0.0	0.0
>10k+	0.002481163631560314	10.067521974397058
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGC	11398	3.5279297014043003	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	11015	3.409382844443619	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCG	10033	3.105432417458269	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	3395	1.0508265780196173	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3003	0.9294940246812698	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCT	2345	0.7258286672919007	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT	2310	0.7149954036009768	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAACCGAT	2100	0.6499958214554336	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTA	1985	0.614400812185255	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTC	1868	0.5781867592755952	No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	728	0.22533188477121693	No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	703	0.2175938392776999	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATG	685	0.2120224465223676	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTT	668	0.206760575586776	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	552	0.17085604449685682	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAACCGATCGT	484	0.1498085607544904	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCAACCGA	454	0.14052290616226992	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTAT	434	0.13433246976745625	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	365	0.11297546420534915	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2225461883935508	0.0
2	0.0	0.0	0.0	1.716608012281826	0.0
3	0.0	0.0	0.0	2.080296150477128	0.0
4	0.0	0.0	0.0	3.2078841397924345	0.0
5	0.0	0.0	0.0	7.768378631851652	0.0
6	0.0	0.0	0.0	8.670634736395742	0.0
7	0.0	0.0	0.0	10.035316439632412	0.0
8	0.0	0.0	0.0	11.534640134456279	0.0
9	0.0	0.0	0.0	11.92061384367291	0.0
10	0.0	0.0	0.0	15.871350350842983	0.0
11	0.0	0.0	0.0	16.99553360014114	0.0
12	0.0	0.0	0.0	21.62845619801968	0.0
13	0.0	0.0	0.0	22.275666323097447	0.0
14	0.0	0.0	0.0	22.6077832356792	0.0
15	0.0	0.0	0.0	23.552134307708023	0.0
16	0.0	0.0	0.0	24.247939358485077	0.0
17	0.0	0.0	0.0	24.93043497101328	0.0
18	0.0	0.0	0.0	25.552883350511795	0.0
19	0.0	0.0	0.0	26.77394692938879	0.0
20	0.0	0.0	0.0	27.39206200341093	0.0
21	0.0	0.0	0.0	27.94146323345064	0.0
22	0.0	0.0	0.0	28.640982546064585	0.0
23	0.0	0.0	0.0	29.217621696241476	0.0
24	0.0	0.0	0.0	29.689023427706537	0.0
25	0.0	0.0	0.0	30.1170921044079	0.0
26	0.0	0.0	0.0	30.512041946397012	0.0
27	0.0	0.0	0.0	30.961777150480223	0.0
28	0.0	0.0	0.0	31.35889364520752	0.0
29	0.0	0.0	0.0	31.72629604523971	0.0
30	0.0	0.0	0.0	32.162412289254334	0.0
31	0.0	0.0	0.0	32.580885789543736	0.0
32	0.0	0.0	0.0	32.975835631532846	0.0
33	0.0	0.0	0.0	33.34695229340193	0.0
34	0.0	0.0	0.0	33.75799727001755	0.0
35	0.0	0.0	0.0	34.14892332835003	0.0
36	0.0	0.0	0.0	34.55842069586696	0.0
37	0.0	0.0	0.0	34.97967989253402	0.0
38	0.0	0.0	0.0	35.39536769644576	0.0
39	0.0	0.0	0.0	35.832722027739344	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.0257485E-4	45.000004	6
ACCGAAC	35	1.2083001E-7	45.000004	42
CGACAGG	40	6.7884685E-9	45.000004	2
ACGGACG	20	7.0257485E-4	45.000004	43
GACGTAG	25	3.8845596E-5	45.0	1
TAGTTGG	25	3.8845596E-5	45.0	2
CACGTGA	25	3.8845596E-5	45.0	43
TCGAATA	25	3.8845596E-5	45.0	44
AACACGT	25	3.8845596E-5	45.0	41
ATTAGTC	30	2.1606393E-6	44.999996	31
TACGGCT	1255	0.0	44.46215	7
CGATGAA	845	0.0	43.136093	19
GATACCT	1065	0.0	42.88732	5
ACGGCTG	1305	0.0	42.758617	8
CGTTTTT	1705	0.0	42.7566	1
ATACCTG	1095	0.0	41.91781	6
ATGAATG	910	0.0	41.785713	21
CGGCTGT	1330	0.0	41.785713	9
TGATACC	1100	0.0	41.727272	4
GATGAAT	915	0.0	41.557377	20
>>END_MODULE