FastQCFastQC Report
Sat 18 Jun 2016
SRR3551731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553774
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC167433.023435553131783No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG156922.833646939003998No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC132692.39610382574841No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC49060.8859209713709925No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47030.8492634179286135No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT35940.6490012171030058No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT31780.5738803194082784No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA31320.5655736816824192No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC28560.5157338553272635No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT27610.49857884263255403No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT13680.24703218280381528No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC10320.1863576115888431No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATG8820.15927074943930197No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT7480.13507315258571187No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGT7310.13200330820876388No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTAT7000.12640535669785868No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC6470.11683466540502081No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGTACCG5940.10726397411218296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG453.8380676E-1045.0000044
GATTGCG453.8380676E-1045.0000041
CTCACGT207.030118E-445.045
GGTACGA207.030118E-445.08
AGATCGC207.030118E-445.029
CGACGGT207.030118E-445.028
TTTCGCG253.8881783E-545.01
TAACGCC253.8881783E-545.012
TCGAATA207.030118E-445.044
ATACCGG207.030118E-445.02
CGTTTTT26500.043.5566061
TACGGCT18600.043.3064547
ACGGCTG18750.042.9600038
CGGCTGT19000.042.6315779
CGATGAA11100.042.56756619
GTCGAAT1550.042.09677543
CGCGCAA650.041.5384641
TGATACC17750.041.4507034
GATACCT17750.041.4507035
CCGATGA11250.041.418