##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553774
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350422735628612	31.0	31.0	33.0	30.0	34.0
2	31.284903227670494	31.0	31.0	34.0	28.0	34.0
3	31.201611126560653	31.0	31.0	34.0	28.0	34.0
4	35.06002268073257	35.0	35.0	37.0	32.0	37.0
5	35.33438189586366	35.0	35.0	37.0	33.0	37.0
6	35.254751938516435	36.0	35.0	37.0	33.0	37.0
7	35.491547093218536	37.0	35.0	37.0	33.0	37.0
8	34.81556374983296	37.0	35.0	37.0	32.0	37.0
9	36.73191951951518	39.0	37.0	39.0	32.0	39.0
10	36.438489347640015	37.0	35.0	39.0	32.0	39.0
11	36.78118871597439	39.0	35.0	39.0	33.0	39.0
12	36.88776829536959	39.0	37.0	39.0	33.0	39.0
13	36.81254627338951	39.0	37.0	39.0	32.0	39.0
14	37.89175006410557	40.0	37.0	41.0	33.0	41.0
15	37.83563330889496	40.0	37.0	41.0	33.0	41.0
16	38.09011076720828	40.0	37.0	41.0	33.0	41.0
17	37.77282790452423	40.0	37.0	41.0	32.0	41.0
18	37.77147175562594	39.0	37.0	41.0	33.0	41.0
19	37.70830699888402	39.0	37.0	41.0	33.0	41.0
20	37.43384304788596	39.0	35.0	41.0	32.0	41.0
21	37.51736809601029	39.0	36.0	41.0	33.0	41.0
22	37.6336032388664	39.0	36.0	41.0	33.0	41.0
23	37.60946162152791	39.0	36.0	41.0	33.0	41.0
24	37.485100420026946	39.0	35.0	41.0	32.0	41.0
25	37.26507745036784	39.0	35.0	41.0	32.0	41.0
26	37.32880922542409	39.0	35.0	41.0	32.0	41.0
27	37.31768735982549	39.0	35.0	41.0	32.0	41.0
28	37.19180026509009	39.0	35.0	41.0	32.0	41.0
29	37.202875180127634	39.0	35.0	41.0	32.0	41.0
30	36.965301729586436	39.0	35.0	41.0	31.0	41.0
31	36.9810717007299	39.0	35.0	41.0	31.0	41.0
32	36.690330351370775	39.0	35.0	41.0	30.0	41.0
33	36.56489651012868	39.0	35.0	41.0	30.0	41.0
34	36.37826441833672	39.0	35.0	41.0	30.0	41.0
35	36.24510359821877	39.0	35.0	41.0	30.0	41.0
36	36.16657336747482	39.0	35.0	41.0	29.0	41.0
37	36.04281349431356	39.0	35.0	41.0	29.0	41.0
38	35.92376673516633	39.0	35.0	41.0	28.0	41.0
39	35.8342085399459	39.0	35.0	40.0	28.0	41.0
40	35.79395565700087	39.0	35.0	40.0	27.0	41.0
41	35.68575086587669	39.0	35.0	40.0	26.0	41.0
42	35.81119554186365	39.0	35.0	40.0	28.0	41.0
43	35.78999194617299	39.0	35.0	41.0	28.0	41.0
44	36.00119904509782	39.0	35.0	41.0	29.0	41.0
45	36.00124238407726	39.0	35.0	41.0	29.0	41.0
46	35.869560506632666	39.0	35.0	40.0	28.0	41.0
47	35.575306894148156	39.0	35.0	40.0	26.0	41.0
48	35.537502663541446	39.0	35.0	40.0	26.0	41.0
49	35.72040579731082	39.0	35.0	40.0	28.0	41.0
50	35.62104396378306	39.0	35.0	40.0	27.0	41.0
51	34.84952164601444	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	15.0
9	22.0
10	21.0
11	20.0
12	20.0
13	19.0
14	12.0
15	30.0
16	35.0
17	73.0
18	170.0
19	394.0
20	964.0
21	1964.0
22	2954.0
23	3652.0
24	4293.0
25	5556.0
26	6871.0
27	7592.0
28	7586.0
29	8093.0
30	9577.0
31	12210.0
32	15624.0
33	21221.0
34	34483.0
35	38617.0
36	45447.0
37	70478.0
38	125290.0
39	130453.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.41497072813097	15.59390653949084	17.048832195083193	16.942290537294998
2	28.598670215647537	35.53055939787711	20.33464915290353	15.536121233571818
3	28.351457453762723	33.80512627895134	24.399303687063675	13.444112580222257
4	25.376778252500117	20.712962327592123	37.64893259705222	16.261326822855533
5	33.46455413219111	23.90253063524109	23.863525553745752	18.769389678822044
6	21.97466836651775	39.76062437023046	25.186267322048344	13.07843994120345
7	64.57327357369613	6.261579633568928	24.008349976705297	5.156796816029644
8	62.6618440013435	16.481994459833796	14.428087992574588	6.4280735462481084
9	57.42775933864718	8.138338022370137	15.27987951763716	19.15402312134553
10	39.627898745697706	21.80275708140866	23.16594856385457	15.403395609039066
11	31.080549104869498	21.811966614539504	28.25141664289042	18.856067637700576
12	24.27849628187672	20.293115964274236	30.764535713124847	24.663852040724194
13	24.445351352717896	21.4934251156609	36.714255273812064	17.34696825780914
14	20.20770205896268	29.531902906239733	28.45095652739204	21.80943850740555
15	16.591244803836943	24.078956397375105	38.80337466186567	20.526424136922284
16	18.45518207788737	26.03083568387104	28.757940965086842	26.75604127315475
17	18.871958596828307	28.288796512656788	32.76733830046192	20.071906590052983
18	18.431706797357766	25.808542835163806	31.755192551474064	24.00455781600436
19	19.298486386143086	26.748456951752882	29.093456897579156	24.85959976452488
20	22.47108026017834	26.135390971768267	35.192695937331834	16.200832830721556
21	20.999721908215268	31.73225900818746	29.612441176364367	17.655577907232914
22	19.08793117770065	23.053447796393474	33.43620321647459	24.422417809431284
23	20.972635046065726	28.547385756644406	31.4817958228447	18.998183374445173
24	21.071411803371053	25.020856883855146	29.269340922470178	24.638390390303623
25	18.144224900410638	33.91690473008845	27.22970020260973	20.709170166891187
26	18.309815917684833	26.091329676005014	32.20284809326549	23.39600631304467
27	23.363863236627218	28.306673841675483	27.343645602718798	20.9858173189785
28	17.49233441801168	27.963573587781298	33.20740952085146	21.336682473355555
29	20.96523130374485	26.71378576820147	29.995088248996883	22.3258946790568
30	18.372838016952763	33.653801009075906	29.442335682065245	18.531025291906083
31	24.202472488777012	24.444267878231916	25.610989320553152	25.742270312437928
32	23.0166096638701	29.720427466800537	28.06090571243865	19.202057156890717
33	21.58497870972635	27.446575678887054	25.289558556378594	25.678887055007998
34	22.177458674477315	26.731843676301164	26.40553727693969	24.685160372281835
35	20.435592859180822	27.686384698450993	25.48115296131635	26.396869481051837
36	24.40147063603564	31.27864435672314	24.16581493533463	20.15407007190659
37	19.38841476847956	28.126455918840538	31.319094070866456	21.166035241813447
38	22.841809113465057	28.07824130421435	24.720915030319226	24.359034552001358
39	22.376276242654946	24.852557180366	30.052692975834905	22.718473601144147
40	18.830967145442003	27.5737033519089	28.45709621614594	25.13823328650316
41	21.72095475771705	26.33583375167487	24.729943984369072	27.21326750623901
42	20.2951023341652	23.643580233091477	31.12099881901281	24.94031861373051
43	25.777483233232328	25.723309508933244	25.48819554547523	23.011011712359196
44	21.692062104757536	24.643085446409547	29.578672888217937	24.08617956061498
45	20.263139836828742	22.737073246486833	28.32744043599013	28.672346480694287
46	25.841408227905248	26.737622206893064	26.68399021983697	20.736979345364716
47	18.120930198962032	25.185003268481367	35.47006540574314	21.224001126813466
48	21.228154445676395	25.82172510807658	27.144105718217176	25.806014728029847
49	19.768714313059117	22.202739745816885	34.9055751985467	23.122970742577298
50	22.00193580774829	22.51839197939954	30.057388031940828	25.422284180911348
51	20.10152155933648	22.2079765391658	28.011607623326483	29.678894278171242
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	424.5
2	742.0
3	4572.0
4	8402.0
5	5726.0
6	3050.0
7	2860.0
8	2670.0
9	2658.0
10	2646.0
11	2535.0
12	2424.0
13	2326.0
14	2228.0
15	2083.5
16	1939.0
17	1851.0
18	1763.0
19	1812.0
20	1861.0
21	2328.0
22	2795.0
23	2593.5
24	2392.0
25	2821.5
26	4154.0
27	5057.0
28	6121.0
29	7185.0
30	8339.0
31	9493.0
32	10366.0
33	11239.0
34	12525.5
35	13812.0
36	14767.0
37	15722.0
38	17080.5
39	18439.0
40	20947.0
41	23455.0
42	24997.5
43	26540.0
44	28773.0
45	31006.0
46	65335.5
47	99665.0
48	75781.5
49	51898.0
50	50128.0
51	48358.0
52	41471.0
53	34584.0
54	30261.5
55	25939.0
56	23691.5
57	21444.0
58	19903.5
59	18363.0
60	17429.0
61	16495.0
62	14635.5
63	12776.0
64	10998.0
65	9220.0
66	7450.0
67	5680.0
68	4834.0
69	3988.0
70	3366.0
71	2744.0
72	2355.0
73	1966.0
74	1557.5
75	897.0
76	645.0
77	489.5
78	334.0
79	260.5
80	187.0
81	141.0
82	95.0
83	54.5
84	14.0
85	10.0
86	6.0
87	4.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.4776499816933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66162657603911	23.92356525018795
2	9.223643028816934	5.991244996920036
3	3.3291638164361967	3.243702514857991
4	1.838918801116911	2.388950446697204
5	1.3487166283922285	2.1901573290706153
6	1.1105243071410005	2.164033184609272
7	0.9541219066478954	2.169134612678334
8	0.8506491668503017	2.2101668718546494
9	0.7699892974196123	2.2506698602119695
>10	6.776896772285132	35.10804231674516
>50	0.08156154746001171	1.7905660558614078
>100	0.04357397741014299	2.4059880906655207
>500	0.0039104851521923195	0.8766861061832286
>1k	0.005027766624247268	4.994861031296416
>5k	0.0	0.0
>10k+	0.0016759222080824226	8.292231332160242
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	16743	3.023435553131783	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	15692	2.833646939003998	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	13269	2.39610382574841	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	4906	0.8859209713709925	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4703	0.8492634179286135	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT	3594	0.6490012171030058	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT	3178	0.5738803194082784	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA	3132	0.5655736816824192	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC	2856	0.5157338553272635	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	2761	0.49857884263255403	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	1368	0.24703218280381528	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	1032	0.1863576115888431	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATG	882	0.15927074943930197	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	748	0.13507315258571187	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGT	731	0.13200330820876388	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTAT	700	0.12640535669785868	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	647	0.11683466540502081	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGTACCG	594	0.10726397411218296	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25389418788169904	0.0
2	0.0	0.0	0.0	1.4948336324926774	0.0
3	0.0	0.0	0.0	1.801998649268474	0.0
4	0.0	0.0	0.0	2.715187061870005	0.0
5	0.0	0.0	0.0	6.143480914596929	0.0
6	0.0	0.0	0.0	6.993827807011525	0.0
7	0.0	0.0	0.0	8.288760396840589	0.0
8	0.0	0.0	0.0	9.632991075781817	0.0
9	0.0	0.0	0.0	9.962728477682232	0.0
10	0.0	0.0	0.0	13.556613347683351	0.0
11	0.0	0.0	0.0	14.647852734147865	0.0
12	0.0	0.0	0.0	18.67639145210862	0.0
13	0.0	0.0	0.0	19.228241123635275	0.0
14	0.0	0.0	0.0	19.51897344404035	0.0
15	0.0	0.0	0.0	20.420243637296082	0.0
16	0.0	0.0	0.0	21.109513989461405	0.0
17	0.0	0.0	0.0	21.8121471936205	0.0
18	0.0	0.0	0.0	22.448327295972724	0.0
19	0.0	0.0	0.0	23.57496018231264	0.0
20	0.0	0.0	0.0	24.14522892010098	0.0
21	0.0	0.0	0.0	24.72922166804509	0.0
22	0.0	0.0	0.0	25.383459678497005	0.0
23	0.0	0.0	0.0	25.95571478617631	0.0
24	0.0	0.0	0.0	26.447973360973972	0.0
25	0.0	0.0	0.0	26.889308634930494	0.0
26	0.0	0.0	0.0	27.284054505989808	0.0
27	0.0	0.0	0.0	27.719791828435426	0.0
28	0.0	0.0	0.0	28.13097039586546	0.0
29	0.0	0.0	0.0	28.531675376597672	0.0
30	0.0	0.0	0.0	28.986012344385976	0.0
31	0.0	0.0	0.0	29.41849924337365	0.0
32	0.0	0.0	0.0	29.86200146630214	0.0
33	0.0	0.0	0.0	30.262525867953354	0.0
34	0.0	0.0	0.0	30.696457399589	0.0
35	0.0	0.0	0.0	31.122443451660786	0.0
36	0.0	0.0	0.0	31.53940054968272	0.0
37	0.0	0.0	0.0	31.960691545648586	0.0
38	0.0	0.0	0.0	32.37150895491663	0.0
39	0.0	0.0	0.0	32.823859552813964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	45	3.8380676E-10	45.000004	4
GATTGCG	45	3.8380676E-10	45.000004	1
CTCACGT	20	7.030118E-4	45.0	45
GGTACGA	20	7.030118E-4	45.0	8
AGATCGC	20	7.030118E-4	45.0	29
CGACGGT	20	7.030118E-4	45.0	28
TTTCGCG	25	3.8881783E-5	45.0	1
TAACGCC	25	3.8881783E-5	45.0	12
TCGAATA	20	7.030118E-4	45.0	44
ATACCGG	20	7.030118E-4	45.0	2
CGTTTTT	2650	0.0	43.556606	1
TACGGCT	1860	0.0	43.306454	7
ACGGCTG	1875	0.0	42.960003	8
CGGCTGT	1900	0.0	42.631577	9
CGATGAA	1110	0.0	42.567566	19
GTCGAAT	155	0.0	42.096775	43
CGCGCAA	65	0.0	41.53846	41
TGATACC	1775	0.0	41.450703	4
GATACCT	1775	0.0	41.450703	5
CCGATGA	1125	0.0	41.4	18
>>END_MODULE