##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408841
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3068405565978	31.0	31.0	33.0	28.0	34.0
2	31.264083103211274	31.0	31.0	34.0	28.0	34.0
3	31.161500436600047	31.0	31.0	34.0	28.0	34.0
4	34.97457935970218	35.0	35.0	37.0	32.0	37.0
5	35.29272749063817	35.0	35.0	37.0	33.0	37.0
6	35.22315521192835	36.0	35.0	37.0	33.0	37.0
7	35.48690811342307	37.0	35.0	37.0	33.0	37.0
8	34.92180089570273	37.0	35.0	37.0	32.0	37.0
9	36.75616682279908	39.0	37.0	39.0	32.0	39.0
10	36.44132804684462	37.0	35.0	39.0	32.0	39.0
11	36.682986784593524	38.0	35.0	39.0	32.0	39.0
12	36.702057768178825	39.0	35.0	39.0	33.0	39.0
13	36.626916087183034	39.0	35.0	39.0	32.0	39.0
14	37.63852206603545	39.0	36.0	41.0	33.0	41.0
15	37.62955036309959	39.0	36.0	41.0	32.0	41.0
16	37.82073715698768	39.0	36.0	41.0	33.0	41.0
17	37.47942843305833	39.0	36.0	41.0	32.0	41.0
18	37.460450395141386	39.0	36.0	40.0	32.0	41.0
19	37.402672432559356	39.0	36.0	40.0	32.0	41.0
20	37.19029402628406	39.0	35.0	40.0	32.0	41.0
21	37.19535956520995	39.0	35.0	40.0	32.0	41.0
22	37.310147465640675	39.0	35.0	41.0	32.0	41.0
23	37.312314567276765	39.0	35.0	41.0	32.0	41.0
24	37.19027445877493	39.0	35.0	41.0	32.0	41.0
25	36.97676847478604	39.0	35.0	40.0	32.0	41.0
26	37.05935559300559	39.0	35.0	40.0	32.0	41.0
27	37.07960796495459	39.0	35.0	41.0	32.0	41.0
28	36.930985395300375	39.0	35.0	41.0	31.0	41.0
29	37.005195173673876	39.0	35.0	41.0	32.0	41.0
30	36.6696759865082	39.0	35.0	41.0	31.0	41.0
31	36.69449492590029	39.0	35.0	40.0	31.0	41.0
32	36.45307589013822	39.0	35.0	40.0	30.0	41.0
33	36.23613091641983	39.0	35.0	41.0	30.0	41.0
34	36.1433711393916	39.0	35.0	41.0	30.0	41.0
35	36.00345611129999	39.0	35.0	41.0	29.0	41.0
36	35.88236258105229	39.0	35.0	41.0	28.0	41.0
37	35.839986694093795	39.0	35.0	41.0	28.0	41.0
38	35.64044212786878	39.0	35.0	41.0	26.0	41.0
39	35.597895025205396	39.0	35.0	40.0	27.0	41.0
40	35.49224759747677	39.0	35.0	40.0	26.0	41.0
41	35.3965991669133	38.0	35.0	40.0	25.0	41.0
42	35.46777598137173	39.0	35.0	40.0	26.0	41.0
43	35.52586947003847	39.0	35.0	40.0	27.0	41.0
44	35.70235372675441	39.0	35.0	41.0	27.0	41.0
45	35.6285475282567	39.0	35.0	40.0	27.0	41.0
46	35.49456634730861	38.0	35.0	40.0	26.0	41.0
47	35.215071384719245	38.0	35.0	40.0	25.0	41.0
48	35.21479499365279	38.0	35.0	40.0	25.0	41.0
49	35.371078243131194	38.0	35.0	40.0	26.0	41.0
50	35.2694715060378	38.0	35.0	40.0	26.0	41.0
51	34.42696060326631	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	20.0
10	19.0
11	21.0
12	10.0
13	11.0
14	18.0
15	18.0
16	34.0
17	65.0
18	130.0
19	298.0
20	659.0
21	1349.0
22	2202.0
23	2804.0
24	3584.0
25	4733.0
26	5628.0
27	5999.0
28	6408.0
29	6735.0
30	7723.0
31	9619.0
32	12520.0
33	17561.0
34	31430.0
35	31839.0
36	30946.0
37	47500.0
38	86282.0
39	92663.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.17266125461977	15.320625866779505	17.58458667305872	17.922126205542007
2	29.83458116969678	33.20777514975259	20.877553865683726	16.080089814866906
3	27.36198179732463	31.971597760498582	26.527672126817027	14.138748315359761
4	25.038829275928787	20.45171594825372	37.59603366589946	16.913421109918037
5	32.3389777444043	23.536289168649915	25.271438040705313	18.85329504624047
6	22.638140499607427	37.49770693252389	26.633092082252023	13.231060485616657
7	65.80969129808409	5.635931817014439	23.19581450979721	5.358562375104258
8	64.23255006224913	14.276454660858379	15.039342923043433	6.451652353849051
9	59.34458628170853	6.780631101088199	15.729831401449463	18.144951215753803
10	42.23646845595231	19.259810048405125	23.13515523149586	15.368566264146697
11	33.97824582172532	21.311218786765515	27.09782042407684	17.61271496743233
12	29.032557889252793	18.333043897260794	29.87542834500454	22.75896986848188
13	26.242231087390945	19.49266340704577	35.682820460765925	18.582285044797366
14	20.641031599081305	27.246288899596667	29.345638035324246	22.767041465997785
15	18.21221452838634	22.42730058873743	39.312593404281856	20.04789147859437
16	21.609134113261636	23.567352589392943	29.3116394882118	25.511873809133622
17	20.47397398988849	25.82030667178683	33.12608079913708	20.579638539187606
18	20.472017238975543	24.136522511196283	32.142324277653174	23.249135972175004
19	21.15247736895272	26.860075188153832	28.89974342103654	23.087704021856908
20	24.414381140834703	25.980760246648455	33.90975953977218	15.69509907274466
21	23.22883468145318	30.23962860867672	29.240218079889246	17.291318629980847
22	20.00386458305307	22.297176653026483	34.17123038051467	23.527728383405773
23	22.508994939352952	26.820940169894897	32.202249774362166	18.46781511638999
24	24.61910620510174	22.255595696126367	28.803618032438038	24.321680066333855
25	19.539136241228253	30.957756193728127	27.155789169872886	22.34731839517074
26	18.51917982785484	22.836750717271507	32.9849501395408	25.659119315332855
27	22.369331842941385	24.702512712766087	29.85439327269036	23.073762171602162
28	16.361617352467096	25.856995751404583	34.49213753023791	23.289249365890406
29	23.07669729797158	22.257552447039313	30.772354044726434	23.89339621026267
30	20.422364684559525	25.976846744822556	35.21393402325109	18.386854547366823
31	23.60697679538011	21.12801798254089	30.133964059377604	25.131041162701393
32	30.205630061564275	23.965551400177574	27.158235108514067	18.67058342974408
33	22.71347540975587	22.38205072387554	30.5517303792917	24.352743487076882
34	22.881022206676924	22.02861259022456	28.908059612416565	26.182305590681953
35	23.61700514380896	23.26968185676094	30.224708382965503	22.88860461646459
36	18.85182748305576	28.91980011789424	29.584117052839616	22.644255346210386
37	23.75006420588933	24.17785887423228	32.336531805763116	19.735545114115265
38	21.875986019014725	25.764783864631973	29.917498489632887	22.44173162672041
39	26.02160742195621	24.171499433765202	26.724570187432278	23.082322956846305
40	23.705548122619795	21.117255852519683	31.723090394554358	23.454105630306156
41	19.078810588957566	27.411145164012417	26.099388270745838	27.410655976284175
42	23.207555015274885	23.25402784945737	30.497675135321558	23.040741999946192
43	21.93395476481077	25.409633573932165	26.88844807639156	25.767963584865512
44	21.42299818266759	21.72947429440785	30.729794712369845	26.117732810554717
45	21.351821368209155	20.305204223646843	29.576534643051943	28.766439765092056
46	26.591755719216025	25.055217064824713	26.967941082230013	21.38508613372925
47	17.84899264017063	23.05492844406505	36.865187199913905	22.230891715850415
48	21.327851169525562	23.21880633302433	29.19961549844561	26.2537269990045
49	21.635794844450533	19.5851198876825	35.50206559518248	23.27701967268449
50	21.5032249700984	20.996181889781113	31.53059502349324	25.969998116627245
51	19.8067219285737	19.830936721121414	29.847055456766814	30.515285893538074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	322.0
2	536.0
3	3697.5
4	6859.0
5	4588.0
6	2317.0
7	2279.5
8	2242.0
9	2165.0
10	2088.0
11	2079.0
12	2070.0
13	1968.0
14	1866.0
15	1728.0
16	1590.0
17	1525.5
18	1461.0
19	1387.0
20	1313.0
21	1530.5
22	1748.0
23	1703.0
24	1658.0
25	1971.5
26	2443.0
27	2601.0
28	3258.5
29	3916.0
30	4154.0
31	4392.0
32	5384.5
33	6377.0
34	7321.5
35	8266.0
36	8896.5
37	9527.0
38	10313.5
39	11100.0
40	12806.0
41	14512.0
42	15573.0
43	16634.0
44	18894.5
45	21155.0
46	43638.0
47	66121.0
48	51290.5
49	36460.0
50	37057.0
51	37654.0
52	32892.0
53	28130.0
54	25426.0
55	22722.0
56	21281.0
57	19840.0
58	19009.0
59	18178.0
60	17352.0
61	16526.0
62	14980.0
63	13434.0
64	11329.5
65	9225.0
66	7146.0
67	5067.0
68	4129.0
69	3191.0
70	2725.5
71	2260.0
72	1807.0
73	1354.0
74	1184.5
75	754.5
76	494.0
77	399.0
78	304.0
79	219.0
80	134.0
81	99.0
82	64.0
83	41.0
84	18.0
85	12.0
86	6.0
87	12.5
88	19.0
89	10.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	408841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.35058538764001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25146252941465	26.248326663032252
2	8.601045037458729	6.081039540392443
3	3.184864147301659	3.377604359616617
4	1.9118067223287516	2.7033394712938685
5	1.5000119303628803	2.651314991338586
6	1.274489419461019	2.70323682289803
7	1.1710237958345262	2.8977463679924718
8	1.0174310623359	2.877342691611401
9	0.922796075166793	2.9359243305506504
>10	6.077038096265505	32.50686444196701
>50	0.05475955544486952	1.346961458982942
>100	0.024260562538866243	1.7397537490956683
>500	0.002079476789045678	0.5523105564030473
>1k	0.004852112507773248	3.985751335604245
>5k	6.931589296818926E-4	2.23840147858999
>10k+	0.0013863178593637852	5.154081740630744
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	10789	2.6389231999726053	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	10245	2.505864137892237	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	9135	2.2343649487208963	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	3554	0.8692865930765261	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3075	0.7521261321638485	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	2443	0.5975428100410674	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGTCGAT	1945	0.4757350657101416	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTA	1849	0.4522540547547824	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	1715	0.4194784769629269	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	1685	0.41214066103937713	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	894	0.2186669145217823	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	852	0.20839397222881267	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATG	508	0.12425368297210897	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGT	479	0.11716046091267754	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	434	0.10615373702735292	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTAT	428	0.10468617384264299	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	413	0.10101726588086811	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22576013658121372	0.0
2	0.0	0.0	0.0	1.507187390697117	0.0
3	0.0	0.0	0.0	1.825893195643294	0.0
4	0.0	0.0	0.0	2.6496853299938117	0.0
5	0.0	0.0	0.0	5.934091737374676	0.0
6	0.0	0.0	0.0	6.672031425419662	0.0
7	0.0	0.0	0.0	7.876656206202411	0.0
8	0.0	0.0	0.0	9.143163234607096	0.0
9	0.0	0.0	0.0	9.483148705731567	0.0
10	0.0	0.0	0.0	12.806690131371365	0.0
11	0.0	0.0	0.0	13.992236590752885	0.0
12	0.0	0.0	0.0	17.61222577970409	0.0
13	0.0	0.0	0.0	18.079644654034208	0.0
14	0.0	0.0	0.0	18.36312894254735	0.0
15	0.0	0.0	0.0	19.134088802248307	0.0
16	0.0	0.0	0.0	19.79033413967777	0.0
17	0.0	0.0	0.0	20.421875496831287	0.0
18	0.0	0.0	0.0	21.061733045364825	0.0
19	0.0	0.0	0.0	22.101990749460057	0.0
20	0.0	0.0	0.0	22.655995851688065	0.0
21	0.0	0.0	0.0	23.24033059306674	0.0
22	0.0	0.0	0.0	23.88923811457266	0.0
23	0.0	0.0	0.0	24.43027974200239	0.0
24	0.0	0.0	0.0	24.90674858930489	0.0
25	0.0	0.0	0.0	25.35190942200024	0.0
26	0.0	0.0	0.0	25.74032447822014	0.0
27	0.0	0.0	0.0	26.186219092507844	0.0
28	0.0	0.0	0.0	26.58955437443896	0.0
29	0.0	0.0	0.0	26.998270721380685	0.0
30	0.0	0.0	0.0	27.440741021570734	0.0
31	2.445938641183247E-4	0.0	0.0	27.861197873990132	0.0
32	2.445938641183247E-4	0.0	0.0	28.255483182948872	0.0
33	2.445938641183247E-4	0.0	0.0	28.686946759253598	0.0
34	2.445938641183247E-4	0.0	0.0	29.11131711349889	0.0
35	2.445938641183247E-4	0.0	0.0	29.5305509965977	0.0
36	2.445938641183247E-4	0.0	0.0	29.947828128783563	0.0
37	2.445938641183247E-4	0.0	0.0	30.3567890695894	0.0
38	2.445938641183247E-4	0.0	0.0	30.821762005278334	0.0
39	2.445938641183247E-4	0.0	0.0	31.321222675807956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	20	7.027948E-4	45.0	34
CCGATCG	145	0.0	45.0	40
GCGAACC	20	7.027948E-4	45.0	33
GCGCGAC	35	1.2093551E-7	45.0	9
CGACAAA	25	3.886381E-5	45.0	12
CTACGAT	25	3.886381E-5	45.0	41
CGAATGT	20	7.027948E-4	45.0	45
GCGATCA	25	3.886381E-5	45.0	33
TATAGCG	20	7.027948E-4	45.0	1
CAATACC	25	3.886381E-5	45.0	17
CTAGCGG	20	7.027948E-4	45.0	2
GCGAATG	30	2.162058E-6	44.999996	1
TTATGCG	30	2.162058E-6	44.999996	1
TTGCGTC	30	2.162058E-6	44.999996	16
CGATCGA	125	0.0	44.999996	41
TACGGCT	1190	0.0	43.487396	7
CGTTTTT	2170	0.0	43.444702	1
GATCGAA	130	0.0	43.269234	42
GATACCT	1115	0.0	42.78027	5
TGATACC	1110	0.0	42.77027	4
>>END_MODULE