FastQCFastQC Report
Sat 18 Jun 2016
SRR3551728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573350
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC113171.9738379698264585No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC108981.900758698875033TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG105831.8458184355105958No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC37980.6624226039940699TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35130.6127147466643412No Hit
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT24060.4196389639836051TruSeq Adapter, Index 27 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT22580.3938257608790442No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCT20040.349524723118514No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA19020.3317345426005058No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC18180.31708380570332256No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC15300.26685270777012293TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT9080.158367489317171TruSeq Adapter, Index 27 (95% over 24bp)
CTGAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC8690.15156536147205024No Hit
GAATCTGTCTCTTATACACATCTGATGCTCTCTCTCTCGTATGCCGTCTTC7340.1280195343158629TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGAGGCTCTCTCTCTCGTATGC7300.1273218801779018No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG5890.10272957181477282No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5790.10098543646987006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.030325E-445.0000041
TCTCGCG207.030325E-445.00000431
AACGACG207.030325E-445.00000413
CTCGAAT1800.045.00000443
TACCGGT207.030325E-445.00000439
TTGGACG253.8883518E-545.01
TTTCGCG253.8883518E-545.01
TTGCGAG253.8883518E-545.01
CGATGAA11200.042.58928319
CGTTTTT22850.042.5382921
CCGATGA11400.041.2518
GATGAAT12500.041.2220
TACGGCT15200.041.1513147
ACGGCTG15300.040.882358
ACGACCA1050.040.71428728
GTACGAG501.0786607E-940.51
CACGTGA950.040.26315743
GCTCGAA451.9254003E-840.00000442
ATGAATG13100.039.6755721
CCACCGA15000.039.44999715
Produced by FastQC (version 0.11.5)