##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573350
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308244527775354	31.0	31.0	33.0	28.0	34.0
2	31.25880177901805	31.0	31.0	34.0	28.0	34.0
3	31.167402110403767	31.0	31.0	34.0	28.0	34.0
4	35.020948809627626	35.0	35.0	37.0	32.0	37.0
5	35.296565797505885	35.0	35.0	37.0	33.0	37.0
6	35.21381704020232	36.0	35.0	37.0	33.0	37.0
7	35.51630766547484	37.0	35.0	37.0	33.0	37.0
8	34.929446237027996	37.0	35.0	37.0	32.0	37.0
9	36.78541030783989	39.0	37.0	39.0	32.0	39.0
10	36.4102816778582	37.0	35.0	39.0	32.0	39.0
11	36.672638004709164	38.0	35.0	39.0	32.0	39.0
12	36.75043516176855	39.0	35.0	39.0	33.0	39.0
13	36.72220807534665	39.0	35.0	39.0	32.0	39.0
14	37.76493067062004	39.0	37.0	41.0	33.0	41.0
15	37.75418156448941	40.0	37.0	41.0	33.0	41.0
16	37.928652655446065	40.0	37.0	41.0	33.0	41.0
17	37.63097758786082	39.0	36.0	41.0	32.0	41.0
18	37.619506409697394	39.0	36.0	41.0	33.0	41.0
19	37.55604430103776	39.0	36.0	40.0	33.0	41.0
20	37.35198220981948	39.0	35.0	41.0	32.0	41.0
21	37.35972268248016	39.0	35.0	41.0	32.0	41.0
22	37.44244876602424	39.0	35.0	41.0	32.0	41.0
23	37.36190459579663	39.0	35.0	41.0	32.0	41.0
24	37.22880439522107	39.0	35.0	41.0	32.0	41.0
25	36.873473445539375	39.0	35.0	40.0	31.0	41.0
26	36.71419377343682	39.0	35.0	40.0	31.0	41.0
27	36.749890991540944	39.0	35.0	41.0	31.0	41.0
28	36.40069591000262	39.0	35.0	41.0	30.0	41.0
29	36.62917764018488	39.0	35.0	41.0	30.0	41.0
30	36.16971657800645	38.0	35.0	40.0	30.0	41.0
31	36.17571814772826	38.0	35.0	40.0	30.0	41.0
32	35.997315775704195	38.0	35.0	40.0	29.0	41.0
33	35.5801360425569	38.0	35.0	40.0	25.0	41.0
34	35.777793668788696	39.0	35.0	41.0	27.0	41.0
35	35.52551670009593	39.0	35.0	41.0	25.0	41.0
36	35.59050492718235	38.0	35.0	41.0	25.0	41.0
37	35.83160722072033	39.0	35.0	41.0	28.0	41.0
38	35.58554983866748	38.0	35.0	40.0	26.0	41.0
39	35.60339059911049	39.0	35.0	40.0	27.0	41.0
40	35.52073253684486	38.0	35.0	40.0	26.0	41.0
41	35.48427487573036	38.0	35.0	40.0	26.0	41.0
42	35.461660416848346	38.0	35.0	40.0	26.0	41.0
43	35.47945931804308	38.0	35.0	40.0	26.0	41.0
44	35.61189325891689	39.0	35.0	40.0	26.0	41.0
45	35.591601988314295	39.0	35.0	40.0	27.0	41.0
46	35.480202319700005	38.0	35.0	40.0	26.0	41.0
47	35.26040638353536	38.0	35.0	40.0	26.0	41.0
48	35.250276445452165	38.0	35.0	40.0	26.0	41.0
49	35.417080317432635	38.0	35.0	40.0	27.0	41.0
50	35.32376907648033	38.0	35.0	40.0	27.0	41.0
51	34.45990930496207	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	19.0
10	23.0
11	16.0
12	19.0
13	19.0
14	17.0
15	28.0
16	36.0
17	118.0
18	207.0
19	418.0
20	840.0
21	1797.0
22	2931.0
23	4344.0
24	5609.0
25	6478.0
26	7575.0
27	8221.0
28	8755.0
29	10227.0
30	11877.0
31	14700.0
32	18347.0
33	24563.0
34	39246.0
35	44024.0
36	49628.0
37	75139.0
38	119926.0
39	118169.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.33391471178163	15.304089997383796	18.37900061044737	18.982994680387197
2	27.29188104996948	32.749280544170226	22.815383273742043	17.143455132118252
3	28.391732798465164	31.847039330252024	25.333914711781635	14.427313159501177
4	25.970175285602163	21.682218540158715	35.450946193424606	16.896659980814512
5	31.789308450335746	25.109793319961625	23.856632074648992	19.244266155053634
6	22.689456701840065	37.436818697130896	25.958663992325803	13.915060608703236
7	68.40027906165518	5.755297811110142	20.47405598674457	5.370367140490102
8	67.08991017702974	13.954129240429058	12.768117205895177	6.187843376646028
9	62.54870497950641	7.1772913578093664	13.651696171622918	16.622307491061306
10	42.02825499258743	21.31891514781547	21.24444056858812	15.40838929100898
11	32.93782157495422	22.673585070201447	25.164035929188106	19.22455742565623
12	26.99066887590477	19.89151478154705	29.220197087293975	23.897619255254206
13	26.410395046655623	20.509810761315077	33.965989360774394	19.113804831254903
14	20.454696084416153	28.594052498473882	28.192378128542778	22.758873288567194
15	18.280108136391384	23.535711171186886	36.22516787302695	21.959012819394786
16	20.462021452864743	24.513299032004884	29.022412139182002	26.002267375948374
17	20.387895700706373	26.510857242522018	31.435248975320484	21.66599808145112
18	20.325106828289876	25.35536757652394	29.34507717798901	24.974448417197173
19	21.16176855323973	27.409610185750417	26.78625621348217	24.64236504752769
20	25.367925351007237	25.91732798465161	31.573733321705767	17.14101334263539
21	23.40071509549141	30.3587686404465	27.877038458184355	18.363477805877736
22	20.43533618208773	23.1049097409959	32.35807098630854	24.101683090607832
23	23.263277230313072	27.356065230661898	30.15854190285166	19.222115636173367
24	23.51983953954827	24.190808406732362	27.976279759309325	24.313072294410045
25	20.268073602511556	31.301648207900932	26.296328595098977	22.133949594488534
26	19.581930757826807	23.509200313944362	32.51731054329816	24.39155838493067
27	23.94785035318741	26.11929885759135	26.967297462283074	22.96555332693817
28	17.60739513386239	27.85924827766635	31.910351443272	22.62300514519927
29	21.92901369146246	23.113456004185924	31.47745705066713	23.480073253684488
30	20.150170053196128	26.708118950030524	30.559693032179297	22.582017964594055
31	22.010988052672886	22.797418679689542	28.599982558646552	26.59161070899102
32	24.249411354321097	25.932676375686754	27.080491846167266	22.73742042382489
33	21.88645678904683	22.67881747623616	28.62370279933723	26.811022935379786
34	20.817999476759397	22.26458533182175	31.201186012034533	25.71622917938432
35	20.078311676986136	22.398360512775792	29.98657015784425	27.536757652393828
36	18.455044911485132	26.278015173977497	30.665736461149386	24.60120345338798
37	19.06793407168396	23.631987442225515	29.33129850876428	27.968779977326243
38	19.18409348565449	24.049533443795237	30.18435510595622	26.58201796459405
39	21.857155315252463	22.99154094357722	27.044039417458794	28.107264323711522
40	20.344292317083806	20.863346995726868	34.40987180605215	24.382488881137178
41	18.121566233539724	24.37219848260225	27.248103252812417	30.25813203104561
42	19.792273480422082	22.531089212522893	33.18217493677509	24.494462370279933
43	21.60879044213831	23.044388244527774	26.85253335658847	28.494287956745445
44	21.41292404290573	22.027731751983954	29.742391209557862	26.816952995552455
45	20.783291183395832	20.691026423650474	29.299206418418066	29.226475974535624
46	24.400627888724166	24.769512514171097	27.018226214354236	23.811633382750504
47	17.87529432283945	23.59727914886195	35.55803610360164	22.969390424696957
48	20.935728612540334	23.43995814075172	29.05049271823493	26.573820528473007
49	19.53239731403157	21.0403767332345	34.78241911572338	24.644806837010552
50	20.247492805441702	20.930845033574606	31.420947065492282	27.400715095491414
51	18.83980116857068	20.63591174675155	29.855934420511034	30.668352664166736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	431.0
2	743.0
3	3823.0
4	6903.0
5	4696.0
6	2489.0
7	2421.0
8	2353.0
9	2249.5
10	2146.0
11	2117.5
12	2089.0
13	1954.0
14	1819.0
15	1793.0
16	1767.0
17	1698.0
18	1629.0
19	1681.5
20	1734.0
21	1882.0
22	2030.0
23	2161.5
24	2293.0
25	2682.5
26	3770.0
27	4468.0
28	5205.5
29	5943.0
30	6987.5
31	8032.0
32	9044.5
33	10057.0
34	11406.5
35	12756.0
36	13515.0
37	14274.0
38	15502.5
39	16731.0
40	18918.5
41	21106.0
42	22788.0
43	24470.0
44	28525.5
45	32581.0
46	58156.0
47	83731.0
48	67014.5
49	50298.0
50	50654.0
51	51010.0
52	45101.0
53	39192.0
54	36323.0
55	33454.0
56	31819.0
57	30184.0
58	28070.0
59	25956.0
60	24579.0
61	23202.0
62	20963.0
63	18724.0
64	15490.5
65	12257.0
66	9938.0
67	7619.0
68	6520.0
69	5421.0
70	4602.0
71	3783.0
72	3212.0
73	2641.0
74	2236.5
75	1462.0
76	1092.0
77	911.5
78	731.0
79	534.5
80	338.0
81	239.0
82	140.0
83	111.5
84	83.0
85	56.5
86	30.0
87	24.0
88	18.0
89	14.0
90	10.0
91	5.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	573350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.376720318131206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5874804916292	23.825212747934145
2	8.80958803392179	5.704511357844822
3	3.237168682203954	3.1442671513899234
4	1.980407068848554	2.564763431366386
5	1.3961462434435397	2.26013182235905
6	1.1000174215722403	2.1368973841989747
7	1.0148294339130794	2.299979412668783
8	0.9081425134718148	2.352214093414532
9	0.7980726807897452	2.32550783815237
>10	7.044834506537121	39.12745017302616
>50	0.07734100037133579	1.7612387525793367
>100	0.034614154012344876	2.244712623713853
>500	0.005408461564428887	1.1427564226816915
>1k	0.0043267692515431095	3.3671564896944908
>5k	0.0	0.0
>10k+	0.001622538469328666	5.743200298975501
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	11317	1.9738379698264585	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	10898	1.900758698875033	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	10583	1.8458184355105958	No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	3798	0.6624226039940699	TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3513	0.6127147466643412	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	2406	0.4196389639836051	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	2258	0.3938257608790442	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCT	2004	0.349524723118514	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA	1902	0.3317345426005058	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC	1818	0.31708380570332256	No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1530	0.26685270777012293	TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	908	0.158367489317171	TruSeq Adapter, Index 27 (95% over 24bp)
CTGAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	869	0.15156536147205024	No Hit
GAATCTGTCTCTTATACACATCTGATGCTCTCTCTCTCGTATGCCGTCTTC	734	0.1280195343158629	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGAGGCTCTCTCTCTCGTATGC	730	0.1273218801779018	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG	589	0.10272957181477282	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	579	0.10098543646987006	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.488270689805529E-4	0.0	0.0	0.1679602337141362	0.0
2	3.488270689805529E-4	0.0	0.0	1.283160373244964	0.0
3	3.488270689805529E-4	0.0	0.0	1.5869887503270255	0.0
4	3.488270689805529E-4	0.0	0.0	2.4086509113107177	0.0
5	3.488270689805529E-4	0.0	0.0	5.529432283945234	0.0
6	3.488270689805529E-4	0.0	0.0	6.329467166652132	0.0
7	3.488270689805529E-4	0.0	0.0	7.508677073340891	0.0
8	3.488270689805529E-4	0.0	0.0	8.666608528821836	0.0
9	3.488270689805529E-4	0.0	0.0	9.004796372198483	0.0
10	3.488270689805529E-4	0.0	0.0	11.688846254469347	0.0
11	3.488270689805529E-4	0.0	0.0	12.672364175460016	0.0
12	3.488270689805529E-4	0.0	0.0	15.950989796808232	0.0
13	3.488270689805529E-4	0.0	0.0	16.415278625621347	0.0
14	3.488270689805529E-4	0.0	0.0	16.692770558995377	0.0
15	3.488270689805529E-4	0.0	0.0	17.33618208773001	0.0
16	3.488270689805529E-4	0.0	0.0	17.8906427138746	0.0
17	3.488270689805529E-4	0.0	0.0	18.51853143803959	0.0
18	3.488270689805529E-4	0.0	0.0	19.088166041684836	0.0
19	3.488270689805529E-4	0.0	0.0	20.028952646725386	0.0
20	3.488270689805529E-4	0.0	0.0	20.513996686142846	0.0
21	3.488270689805529E-4	0.0	0.0	21.04735327461411	0.0
22	3.488270689805529E-4	0.0	0.0	21.69111363041772	0.0
23	3.488270689805529E-4	0.0	0.0	22.200052324060348	0.0
24	3.488270689805529E-4	0.0	0.0	22.63085375425133	0.0
25	3.488270689805529E-4	0.0	0.0	23.011598500043604	0.0
26	3.488270689805529E-4	0.0	0.0	23.37472747885236	0.0
27	3.488270689805529E-4	0.0	0.0	23.778494811197348	0.0
28	3.488270689805529E-4	0.0	0.0	24.11371762448766	0.0
29	3.488270689805529E-4	0.0	0.0	24.476672189761924	0.0
30	3.488270689805529E-4	0.0	0.0	24.87119560477893	0.0
31	3.488270689805529E-4	0.0	0.0	25.24949856108834	0.0
32	3.488270689805529E-4	0.0	0.0	25.629894479811632	0.0
33	3.488270689805529E-4	0.0	0.0	25.998430278189588	0.0
34	3.488270689805529E-4	0.0	0.0	26.36679166303305	0.0
35	3.488270689805529E-4	0.0	0.0	26.744920205807972	0.0
36	3.488270689805529E-4	0.0	0.0	27.109095665823666	0.0
37	3.488270689805529E-4	0.0	0.0	27.510072381616812	0.0
38	3.488270689805529E-4	0.0	0.0	27.908258480858116	0.0
39	3.488270689805529E-4	0.0	0.0	28.328943926048662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.030325E-4	45.000004	1
TCTCGCG	20	7.030325E-4	45.000004	31
AACGACG	20	7.030325E-4	45.000004	13
CTCGAAT	180	0.0	45.000004	43
TACCGGT	20	7.030325E-4	45.000004	39
TTGGACG	25	3.8883518E-5	45.0	1
TTTCGCG	25	3.8883518E-5	45.0	1
TTGCGAG	25	3.8883518E-5	45.0	1
CGATGAA	1120	0.0	42.589283	19
CGTTTTT	2285	0.0	42.538292	1
CCGATGA	1140	0.0	41.25	18
GATGAAT	1250	0.0	41.22	20
TACGGCT	1520	0.0	41.151314	7
ACGGCTG	1530	0.0	40.88235	8
ACGACCA	105	0.0	40.714287	28
GTACGAG	50	1.0786607E-9	40.5	1
CACGTGA	95	0.0	40.263157	43
GCTCGAA	45	1.9254003E-8	40.000004	42
ATGAATG	1310	0.0	39.67557	21
CCACCGA	1500	0.0	39.449997	15
>>END_MODULE