SRR3551727_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495773
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	10460	2.10983655826356	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	9774	1.971466780159468	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	8983	1.8119179543863824	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3318	0.6692579063402	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	2843	0.5734479287899906	TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	2297	0.46331688091122347	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	2003	0.4040155474380412	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	1904	0.3840467310644186	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	1593	0.32131640892101826	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	1475	0.2975151934453873	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	874	0.17629035869238543	TruSeq Adapter, Index 15 (95% over 23bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	759	0.15309425886443997	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	658	0.132722032059027	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	525	0.10589523834496836	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATG	516	0.10407989140191176	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAA	35	1.2100645E-7	45.000004	12
TCACGAC	25	3.8875856E-5	45.0	25
ACGTTAG	25	3.8875856E-5	45.0	1
CTATCGA	25	3.8875856E-5	45.0	41
ACCTAGA	25	3.8875856E-5	45.0	30
CGTACGT	20	7.0294004E-4	45.0	8
TTTGACG	20	7.0294004E-4	45.0	1
CCAGTCG	20	7.0294004E-4	45.0	28
CGATATT	20	7.0294004E-4	45.0	9
TATCGAG	20	7.0294004E-4	45.0	1
GCGAGTA	20	7.0294004E-4	45.0	26
TCGAATA	60	0.0	44.999996	44
CGATCGA	170	0.0	43.676468	41
TCGAATG	220	0.0	42.954544	44
CGATGAA	1640	0.0	42.667683	19
CCGATGA	1625	0.0	42.36923	18
CCGATCG	85	0.0	42.35294	40
TACGGCT	1175	0.0	42.31915	7
CGTTTTT	1790	0.0	42.23464	1
ACGGCTG	1180	0.0	42.139828	8