##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495773
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2355654704875	31.0	31.0	33.0	28.0	34.0
2	31.180009802873492	31.0	31.0	34.0	28.0	34.0
3	31.10299068323608	31.0	31.0	34.0	28.0	34.0
4	34.93375193889139	35.0	35.0	37.0	32.0	37.0
5	35.20763333219034	35.0	35.0	37.0	33.0	37.0
6	35.120770998017235	36.0	35.0	37.0	32.0	37.0
7	35.44260982344743	37.0	35.0	37.0	33.0	37.0
8	34.80620969677655	37.0	35.0	37.0	32.0	37.0
9	36.55336414044331	39.0	35.0	39.0	32.0	39.0
10	36.27142865787366	37.0	35.0	39.0	32.0	39.0
11	36.58511052437305	38.0	35.0	39.0	32.0	39.0
12	36.645906493496014	39.0	35.0	39.0	32.0	39.0
13	36.60251365040049	39.0	35.0	39.0	32.0	39.0
14	37.670070778360255	39.0	37.0	41.0	32.0	41.0
15	37.700138974893754	40.0	36.0	41.0	32.0	41.0
16	37.8699102210084	39.0	37.0	41.0	33.0	41.0
17	37.552966781167996	39.0	36.0	41.0	32.0	41.0
18	37.57228207264212	39.0	36.0	41.0	33.0	41.0
19	37.48895563090366	39.0	36.0	40.0	32.0	41.0
20	37.20618105463589	39.0	35.0	41.0	31.0	41.0
21	37.260193677348305	39.0	35.0	40.0	32.0	41.0
22	37.317324662698454	39.0	35.0	41.0	32.0	41.0
23	37.267769725257324	39.0	35.0	41.0	32.0	41.0
24	37.16100312037969	39.0	35.0	41.0	31.0	41.0
25	36.970409844828175	39.0	35.0	40.0	31.0	41.0
26	36.981646842405695	39.0	35.0	41.0	31.0	41.0
27	36.929495958836	39.0	35.0	41.0	31.0	41.0
28	36.78818935278847	39.0	35.0	41.0	30.0	41.0
29	36.828596958688756	39.0	35.0	41.0	31.0	41.0
30	36.51429384012441	39.0	35.0	41.0	30.0	41.0
31	36.604528685507276	39.0	35.0	40.0	30.0	41.0
32	36.28712737482679	39.0	35.0	41.0	30.0	41.0
33	36.24126969399302	39.0	35.0	41.0	29.0	41.0
34	36.1146109207238	39.0	35.0	41.0	29.0	41.0
35	35.88486262866272	39.0	35.0	41.0	27.0	41.0
36	35.777948778977475	39.0	35.0	41.0	27.0	41.0
37	35.668434949059346	39.0	35.0	41.0	26.0	41.0
38	35.49308655372519	39.0	35.0	40.0	25.0	41.0
39	35.45129525004387	39.0	35.0	40.0	26.0	41.0
40	35.32786174317682	38.0	35.0	40.0	25.0	41.0
41	35.218132088677685	38.0	35.0	40.0	24.0	41.0
42	35.12730422996008	38.0	35.0	40.0	24.0	41.0
43	35.23385299320455	38.0	35.0	40.0	25.0	41.0
44	35.4834107545187	38.0	35.0	40.0	26.0	41.0
45	35.42839969098761	38.0	35.0	40.0	26.0	41.0
46	35.26697298965454	38.0	35.0	40.0	26.0	41.0
47	34.989438714895726	38.0	34.0	40.0	24.0	41.0
48	34.94415589392726	38.0	34.0	40.0	24.0	41.0
49	35.01117446896059	38.0	34.0	40.0	25.0	41.0
50	34.95939068888382	38.0	34.0	40.0	25.0	41.0
51	34.06047929193401	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	30.0
10	30.0
11	37.0
12	16.0
13	23.0
14	30.0
15	35.0
16	66.0
17	115.0
18	226.0
19	635.0
20	1488.0
21	2895.0
22	3933.0
23	4601.0
24	5233.0
25	5966.0
26	6464.0
27	6775.0
28	7065.0
29	7760.0
30	9145.0
31	11338.0
32	14546.0
33	20236.0
34	33145.0
35	36913.0
36	42258.0
37	62187.0
38	102670.0
39	109884.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.22912905704829	15.451022948002413	17.299046136034033	19.02080185891527
2	27.716918831804072	33.764242909557396	21.785575253190473	16.733263005448055
3	29.07762221823294	32.212121273244	24.337751349912157	14.372505158610895
4	26.32495113691145	21.322661782711037	35.27683032355534	17.075556756822174
5	32.963069792021756	24.876707686784073	23.164028698618118	18.996193822576057
6	23.501279819594856	38.63663410472131	23.64872633241423	14.213359743269601
7	67.5617671797375	6.313171552303171	20.69132445695913	5.433736811000196
8	66.90541840721458	14.969754302876517	11.99440066320675	6.13042662670214
9	61.81195829542956	7.889699519739881	12.93515379014186	17.3631883946887
10	42.58420688500584	21.17622379597114	20.333499403961085	15.906069915061932
11	33.75456105919443	22.812053096881034	24.7919914961081	18.64139434781644
12	26.22288829766849	19.859693851823316	28.12819576701434	25.789222083493858
13	26.70012283847648	21.816032740790643	32.46505961397656	19.018784806756315
14	20.612256012328224	30.33908663844138	26.359241023613627	22.689416325616765
15	18.465910809987633	23.624320001290915	34.28403725091927	23.62573193780218
16	20.585025808182376	24.659067758833174	27.1678772341374	27.588029198847053
17	20.107791267374385	28.852922607725713	29.23152329796096	21.80776282693894
18	19.915969607058067	25.24945892575836	28.54471703783788	26.289854429345688
19	21.996155498585036	25.21496733384029	26.37920983998725	26.40966732758742
20	26.360249549693105	24.842014389650103	30.92746075320762	17.870275307449177
21	23.141236009221963	30.830843954793828	27.301809497491796	18.726110538492417
22	21.829143579823832	21.95420081367884	32.131439186885935	24.085216419611395
23	25.43462431395013	25.956234002255062	28.292787223184806	20.316354460609997
24	22.602683082781837	26.747926974643637	27.362926177908037	23.28646376466649
25	20.62032422096403	32.85838478497215	25.3900474612373	21.131243532826517
26	21.314593574075232	24.42710676055372	30.643258104011313	23.615041561359735
27	26.45323565422078	25.502800676922703	26.874597850225808	21.169365818630702
28	20.508982941789892	28.028351685146223	29.561916441597262	21.90074893146662
29	24.43981418915512	22.48448382626726	29.946769993525262	23.128931991052358
30	20.931151958658496	28.890238072666317	30.005264506134864	20.173345462540315
31	26.405834928485415	22.884868679819192	24.367805427080537	26.341490964614856
32	26.704560353226174	26.049421811998634	25.486462554435196	21.759555280339995
33	24.337751349912157	26.59382418969972	24.262313599167364	24.80611086122076
34	27.722969988280926	23.050468662069132	25.22364065812378	24.00292069152616
35	24.66471550487824	22.975434321756126	29.046963025416872	23.312887147948757
36	26.03449562602239	29.621621185502235	24.371436120966653	19.97244706750872
37	21.287565075145277	24.19292700489942	30.380839618131684	24.138668301823618
38	22.91169547353325	26.594832715779198	24.78271305617692	25.710758754510632
39	25.25329132486037	24.907971995247824	25.426354400098433	24.412382279793373
40	21.229272267751572	25.463871570254938	29.922565367617842	23.38429079437565
41	22.760618266827763	27.107365669368843	25.649238663662604	24.48277740014079
42	22.209156206570345	23.56542207824952	31.468434142238483	22.756987572941647
43	23.779229607098408	27.527315928862606	25.19963773743225	23.493816726606735
44	23.59850173365633	23.17653442200362	29.3263247494317	23.898639094908354
45	22.588160307237384	22.42901489189609	27.21225238163434	27.770572419232188
46	26.52403418499999	26.17468075106954	25.468712495436417	21.83257256849405
47	20.010569353312906	26.271700959915123	32.83196140168989	20.885768285082086
48	23.015775364935163	25.61918458649422	26.83566874355804	24.529371305012575
49	21.81825149816549	22.777561504962957	32.712148503448155	22.692038493423404
50	22.35781295068509	24.346626379411546	29.013883370010063	24.281677299893296
51	22.32453159006239	22.73883410351108	27.174533506261938	27.76210080016459
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	427.0
2	783.0
3	3286.0
4	5789.0
5	4027.0
6	2265.0
7	2196.0
8	2127.0
9	2036.0
10	1945.0
11	1814.0
12	1683.0
13	1650.5
14	1618.0
15	1487.5
16	1357.0
17	1264.5
18	1172.0
19	1231.0
20	1290.0
21	2036.5
22	2783.0
23	2515.0
24	2247.0
25	2520.0
26	3214.0
27	3635.0
28	4468.0
29	5301.0
30	6292.5
31	7284.0
32	7989.5
33	8695.0
34	10017.5
35	11340.0
36	11971.5
37	12603.0
38	13698.0
39	14793.0
40	16432.0
41	18071.0
42	19742.5
43	21414.0
44	23899.5
45	26385.0
46	48239.5
47	70094.0
48	54296.0
49	38498.0
50	38968.0
51	39438.0
52	35082.0
53	30726.0
54	29361.5
55	27997.0
56	26478.5
57	24960.0
58	23282.5
59	21605.0
60	20365.0
61	19125.0
62	17794.0
63	16463.0
64	14734.0
65	13005.0
66	11279.5
67	9554.0
68	8489.0
69	7424.0
70	6941.5
71	6459.0
72	5599.5
73	4740.0
74	3981.0
75	2603.5
76	1985.0
77	1697.5
78	1410.0
79	1124.5
80	839.0
81	656.5
82	474.0
83	318.0
84	162.0
85	128.5
86	95.0
87	64.0
88	33.0
89	22.5
90	12.0
91	6.5
92	1.0
93	4.0
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.577820867578332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.58387078002511	22.19456598587061
2	8.133024086276171	4.973803072437054
3	3.18162184283904	2.9186118833611987
4	1.990324539448188	2.4343914894236818
5	1.53982976081746	2.3542319296421144
6	1.253761070755712	2.3002368859386824
7	1.1285857370692047	2.4156784751264198
8	1.0398156866347075	2.543623824097124
9	0.9490001551528333	2.611652107281065
>10	8.07318140387249	41.76348610844631
>50	0.0780428259316317	1.629614103785915
>100	0.03902141296581585	2.1564377250768447
>500	0.003306899403882699	0.6738488699199143
>1k	0.004629659165435779	3.1211013233715597
>5k	0.0013227597615530797	3.793332308849889
>10k+	6.613798807765398E-4	2.1153839073716396
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	10460	2.10983655826356	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	9774	1.971466780159468	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	8983	1.8119179543863824	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3318	0.6692579063402	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	2843	0.5734479287899906	TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	2297	0.46331688091122347	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	2003	0.4040155474380412	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	1904	0.3840467310644186	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	1593	0.32131640892101826	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	1475	0.2975151934453873	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	874	0.17629035869238543	TruSeq Adapter, Index 15 (95% over 23bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	759	0.15309425886443997	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	658	0.132722032059027	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	525	0.10589523834496836	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATG	516	0.10407989140191176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0170521589517784E-4	0.0	0.0	0.1109378687423478	0.0
2	2.0170521589517784E-4	0.0	0.0	0.9599151224451513	0.0
3	2.0170521589517784E-4	0.0	0.0	1.1396344698077547	0.0
4	2.0170521589517784E-4	0.0	0.0	1.830676539464634	0.0
5	2.0170521589517784E-4	0.0	0.0	4.5466372714932035	0.0
6	2.0170521589517784E-4	0.0	0.0	5.165468873859609	0.0
7	2.0170521589517784E-4	0.0	0.0	5.965028349668094	0.0
8	2.0170521589517784E-4	0.0	0.0	6.800491353905921	0.0
9	2.0170521589517784E-4	0.0	0.0	7.014500587970704	0.0
10	2.0170521589517784E-4	0.0	0.0	9.320999731732062	0.0
11	2.0170521589517784E-4	0.0	0.0	9.97573486252781	0.0
12	2.0170521589517784E-4	0.0	0.0	12.632595966299093	0.0
13	2.0170521589517784E-4	0.0	0.0	12.980940874150065	0.0
14	2.0170521589517784E-4	0.0	0.0	13.168325019716685	0.0
15	2.0170521589517784E-4	0.0	0.0	13.665931787330088	0.0
16	2.0170521589517784E-4	0.0	0.0	14.063492767859485	0.0
17	2.0170521589517784E-4	0.0	0.0	14.493931698579793	0.0
18	2.0170521589517784E-4	0.0	0.0	14.901577939903948	0.0
19	2.0170521589517784E-4	0.0	0.0	15.668057760305624	0.0
20	4.034104317903557E-4	0.0	0.0	16.031328854132838	0.0
21	4.034104317903557E-4	0.0	0.0	16.373622605506956	0.0
22	4.034104317903557E-4	0.0	0.0	16.822820121305515	0.0
23	4.034104317903557E-4	0.0	0.0	17.16148317879352	0.0
24	4.034104317903557E-4	0.0	0.0	17.456981320079954	0.0
25	4.034104317903557E-4	0.0	0.0	17.732107234560978	0.0
26	4.034104317903557E-4	0.0	0.0	18.00864508555327	0.0
27	6.051156476855335E-4	0.0	0.0	18.337021177030618	0.0
28	6.051156476855335E-4	0.0	0.0	18.608112987193735	0.0
29	6.051156476855335E-4	0.0	0.0	18.868514420914412	0.0
30	6.051156476855335E-4	0.0	0.0	19.168651782166435	0.0
31	6.051156476855335E-4	0.0	0.0	19.459712408703176	0.0
32	6.051156476855335E-4	0.0	0.0	19.742906531820008	0.0
33	6.051156476855335E-4	0.0	0.0	20.014603457630812	0.0
34	6.051156476855335E-4	0.0	0.0	20.270769081817686	0.0
35	6.051156476855335E-4	0.0	0.0	20.558199014468315	0.0
36	6.051156476855335E-4	0.0	0.0	20.851074987948113	0.0
37	6.051156476855335E-4	0.0	0.0	21.13729468930337	0.0
38	6.051156476855335E-4	0.0	0.0	21.416858118534087	0.0
39	6.051156476855335E-4	0.0	0.0	21.707918745070828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAA	35	1.2100645E-7	45.000004	12
TCACGAC	25	3.8875856E-5	45.0	25
ACGTTAG	25	3.8875856E-5	45.0	1
CTATCGA	25	3.8875856E-5	45.0	41
ACCTAGA	25	3.8875856E-5	45.0	30
CGTACGT	20	7.0294004E-4	45.0	8
TTTGACG	20	7.0294004E-4	45.0	1
CCAGTCG	20	7.0294004E-4	45.0	28
CGATATT	20	7.0294004E-4	45.0	9
TATCGAG	20	7.0294004E-4	45.0	1
GCGAGTA	20	7.0294004E-4	45.0	26
TCGAATA	60	0.0	44.999996	44
CGATCGA	170	0.0	43.676468	41
TCGAATG	220	0.0	42.954544	44
CGATGAA	1640	0.0	42.667683	19
CCGATGA	1625	0.0	42.36923	18
CCGATCG	85	0.0	42.35294	40
TACGGCT	1175	0.0	42.31915	7
CGTTTTT	1790	0.0	42.23464	1
ACGGCTG	1180	0.0	42.139828	8
>>END_MODULE