FastQCFastQC Report
Sat 18 Jun 2016
SRR3551726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419398
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC97712.329767905426349No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG86862.0710637628219493No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC85872.047458500040534No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC31100.7415390631333483No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.6621395428685878No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT23140.5517432128908578No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT22170.5286148241050268No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTA17760.4234641080787223No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCTGAAT17120.40820414022002965No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTC16780.40009728229509917No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC11430.2725334884763399No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT9910.23629106481194473No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT6490.15474561156705566No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC5120.12207974286954158No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATG5000.1192184988960367No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA4300.10252790905059157No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGT4230.10085885006604704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC253.886554E-545.025
AACCGTA207.028156E-445.040
TACGCGG253.886554E-545.02
TCTACGG207.028156E-445.02
CGGATGG253.886554E-545.02
CGAATGC1100.045.045
CGTACGA207.028156E-445.023
GCTACGA253.886554E-545.010
TTACCGG207.028156E-445.02
CAATCGA502.1827873E-1145.041
CCGTTAG207.028156E-445.01
GCGAACC302.1621927E-644.99999633
ATCGAAT1450.043.44827343
CCGATGA12400.042.6411318
ACGGGCG800.042.18755
CGGGAGT800.042.18756
CGTTTTT18300.041.926231
CGATGAA12850.041.67315319
CCGATCG603.6379788E-1241.24999640
CGATCGA603.6379788E-1241.24999641