##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551726_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419398 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.211829813208457 31.0 31.0 33.0 28.0 34.0 2 31.14991010925183 31.0 31.0 34.0 28.0 34.0 3 31.056166219199902 31.0 31.0 34.0 28.0 34.0 4 34.894527394026674 35.0 35.0 37.0 32.0 37.0 5 35.182311312881794 35.0 35.0 37.0 33.0 37.0 6 35.09810251837157 36.0 35.0 37.0 32.0 37.0 7 35.40757943528581 37.0 35.0 37.0 33.0 37.0 8 34.86555491442496 37.0 35.0 37.0 32.0 37.0 9 36.63294770122891 39.0 37.0 39.0 32.0 39.0 10 36.304495968030366 37.0 35.0 39.0 32.0 39.0 11 36.581397622306255 38.0 35.0 39.0 32.0 39.0 12 36.616662454279705 39.0 35.0 39.0 32.0 39.0 13 36.560028898564134 39.0 35.0 39.0 32.0 39.0 14 37.48511676259782 39.0 36.0 41.0 32.0 41.0 15 37.50075822965298 39.0 36.0 41.0 32.0 41.0 16 37.69434999690032 39.0 36.0 41.0 33.0 41.0 17 37.43492339019261 39.0 36.0 41.0 32.0 41.0 18 37.41956089442487 39.0 36.0 40.0 32.0 41.0 19 37.3584018044912 39.0 36.0 40.0 32.0 41.0 20 37.09915640990181 39.0 35.0 40.0 31.0 41.0 21 37.09569907343383 39.0 35.0 40.0 32.0 41.0 22 37.22355376038989 39.0 35.0 41.0 32.0 41.0 23 37.22133152757047 39.0 35.0 41.0 32.0 41.0 24 37.13889670432382 39.0 35.0 41.0 32.0 41.0 25 36.90870247354542 39.0 35.0 40.0 31.0 41.0 26 36.97097983299873 39.0 35.0 40.0 31.0 41.0 27 36.92880271245929 39.0 35.0 41.0 31.0 41.0 28 36.778799612778315 39.0 35.0 41.0 31.0 41.0 29 36.82750752268728 39.0 35.0 41.0 31.0 41.0 30 36.490221698720546 39.0 35.0 40.0 30.0 41.0 31 36.50165713713466 39.0 35.0 40.0 30.0 41.0 32 36.24469120024416 39.0 35.0 40.0 30.0 41.0 33 36.19257602563675 39.0 35.0 41.0 30.0 41.0 34 35.982207831224756 39.0 35.0 41.0 29.0 41.0 35 35.90351170010348 39.0 35.0 41.0 28.0 41.0 36 35.71541590565525 39.0 35.0 40.0 27.0 41.0 37 35.6825163686999 39.0 35.0 40.0 27.0 41.0 38 35.43748181917892 38.0 35.0 40.0 26.0 41.0 39 35.43376458638334 38.0 35.0 40.0 26.0 41.0 40 35.297197411527954 38.0 35.0 40.0 25.0 41.0 41 35.1235747428457 38.0 34.0 40.0 24.0 41.0 42 35.11784748615873 38.0 35.0 40.0 24.0 41.0 43 35.224466974091435 38.0 35.0 40.0 25.0 41.0 44 35.432195670937865 38.0 35.0 40.0 26.0 41.0 45 35.31011592806833 38.0 35.0 40.0 26.0 41.0 46 35.22528481299386 38.0 35.0 40.0 26.0 41.0 47 34.917591404823106 38.0 34.0 40.0 25.0 41.0 48 34.93119423554714 38.0 34.0 40.0 25.0 41.0 49 35.02947319729708 38.0 34.0 40.0 26.0 41.0 50 34.954320239962996 38.0 34.0 40.0 26.0 41.0 51 34.06492877886876 37.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 17.0 10 18.0 11 25.0 12 16.0 13 24.0 14 24.0 15 33.0 16 58.0 17 95.0 18 191.0 19 451.0 20 1097.0 21 2121.0 22 2943.0 23 3535.0 24 4277.0 25 4976.0 26 5575.0 27 5944.0 28 6157.0 29 6879.0 30 7981.0 31 10398.0 32 13554.0 33 18675.0 34 30392.0 35 31291.0 36 35401.0 37 52341.0 38 87292.0 39 87607.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.81138679726656 15.878234993967544 17.548486163501018 18.76189204526488 2 28.71973638405524 33.91265575896881 21.459568238284398 15.908039618691552 3 28.4743847133272 32.2524189433426 24.749283496821633 14.523912846508566 4 25.62124759774725 21.20920939060272 35.72286944620623 17.446673565443803 5 31.85184478705192 23.863728487021874 24.611228475100024 19.673198250826186 6 22.49963042265342 37.85974182041879 25.82606497885064 13.81456277807715 7 67.25258584924106 5.556774233544271 21.85537365461924 5.335266262595435 8 66.09473578796275 13.723956718916162 13.585901697194549 6.595405795926543 9 61.686512572782895 6.12473116228499 14.610703913704882 17.578052351227235 10 42.24579039480398 19.58306906566078 21.8265227778864 16.344617761648838 11 33.314655768506285 20.92141593426769 26.234745993066248 19.52918230415977 12 25.742135155627828 19.053977367560172 30.153219614781186 25.050667862030817 13 27.206376759068952 19.753551519082112 35.06263739931998 17.977434322528957 14 21.05470221603346 27.943147082246455 29.38139905292824 21.62075164879184 15 17.34653002637113 22.28909055360302 37.83017563269257 22.534203787333272 16 20.78789121550413 24.102403921811742 28.058789026175614 27.050915836508523 17 21.484127249056982 27.07475953628773 29.90071483411938 21.540398380535912 18 21.515124058769953 23.34894300878879 30.545210039151353 24.590722893289907 19 21.834391198813538 24.98915111660046 27.50036957734658 25.676088107239426 20 25.164402309977635 26.114096872183463 31.44530970581643 17.27619111202247 21 22.260001239872388 30.181832054516235 29.626750723656286 17.931415981955087 22 20.796236510426848 21.376592163052756 33.42815177945531 24.39901954706508 23 23.183467732321088 26.061879169667 31.157516249481397 19.597136848530514 24 22.695148760842923 25.07427312481223 27.83322762626431 24.397350488080534 25 20.957658357932083 31.236438895750574 26.376854443750332 21.429048302567015 26 21.289085784863065 22.544218141240542 31.335867123829868 24.830828950066525 27 26.27361122370636 24.02658095651386 27.384727633417423 22.31508018636236 28 19.114063491003773 25.80555939704052 31.45103219376344 23.629344918192267 29 25.883528295318527 22.141021177974142 29.07619969575439 22.899250830952937 30 24.371360855321196 25.918101659998378 30.260993137783203 19.44954434689722 31 24.7592978507289 21.436201412500775 28.295795401980932 25.50870533478939 32 27.42931535200454 25.1894381947458 25.975088102470682 21.406158350778973 33 25.718291455848618 21.638395986628453 28.102661433769356 24.54065112375357 34 27.368752354565352 22.140305866980768 27.79650832860433 22.694433449849548 35 25.611948554833358 26.21018698229367 25.620055412758287 22.557809050114688 36 25.413807409668145 28.926938135136552 27.10361041302057 18.555644042174734 37 24.213277125785055 24.611228475100024 27.98940385981812 23.186090539296803 38 20.880643207645242 25.57236801319987 28.492744362157186 25.054244416997694 39 25.363974077129598 23.59286405753008 26.435033071211596 24.608128794128728 40 22.932155136648245 23.615754009318117 30.999432519945252 22.452658334088387 41 23.005832168965995 26.829407865559684 25.610517932846605 24.55424203262772 42 22.280029947686923 25.89187359024125 29.692797772044692 22.135298690027135 43 23.805311422562816 25.573560198188833 26.39664471456707 24.22448366468128 44 24.058054640222416 21.300530760757084 29.887600799240815 24.753813799779685 45 22.772640785125347 20.49175246424637 28.05497403421094 28.680632716417342 46 27.057592072446695 24.559726083576937 26.28648682158713 22.096195022389235 47 19.485786770561614 24.77074282662292 34.4331637251489 21.31030667766656 48 22.414985288437236 24.811277116247574 27.92240306343855 24.851334531876642 49 22.420469339386454 21.615506034840415 33.49109914687242 22.47292547890071 50 22.676073801019555 22.247840952984994 30.404293773456242 24.671791472539212 51 22.165818625744517 20.693708601376258 28.428366372753327 28.712106400125897 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 95.0 1 334.5 2 574.0 3 2755.5 4 4937.0 5 3342.5 6 1748.0 7 1741.5 8 1735.0 9 1677.0 10 1619.0 11 1638.0 12 1657.0 13 1574.0 14 1491.0 15 1428.0 16 1365.0 17 1376.0 18 1387.0 19 1337.5 20 1288.0 21 1357.5 22 1427.0 23 1508.0 24 1589.0 25 1878.5 26 2523.0 27 2878.0 28 3380.0 29 3882.0 30 4305.0 31 4728.0 32 5277.5 33 5827.0 34 6939.5 35 8052.0 36 9116.5 37 10181.0 38 10888.0 39 11595.0 40 12831.5 41 14068.0 42 14925.5 43 15783.0 44 17950.0 45 20117.0 46 41368.5 47 62620.0 48 50228.0 49 37836.0 50 38757.5 51 39679.0 52 34918.5 53 30158.0 54 28188.0 55 26218.0 56 24654.0 57 23090.0 58 21282.5 59 19475.0 60 17714.5 61 15954.0 62 14397.5 63 12841.0 64 11402.5 65 9964.0 66 8297.0 67 6630.0 68 5929.5 69 5229.0 70 4442.5 71 3656.0 72 2981.0 73 2306.0 74 1913.0 75 1236.0 76 952.0 77 752.5 78 553.0 79 413.0 80 273.0 81 202.5 82 132.0 83 109.0 84 86.0 85 55.0 86 24.0 87 16.0 88 8.0 89 5.0 90 2.0 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 419398.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.45167299303059 #Duplication Level Percentage of deduplicated Percentage of total 1 73.1222239964024 28.116718456327987 2 8.709016948332607 6.697525435760932 3 3.776116654071108 4.355940082976371 4 2.5159344790361784 3.869675594391596 5 2.0128024104480406 3.8697810043065903 6 1.705904846446415 3.9356937187670167 7 1.404914464756297 3.781491810739141 8 1.1863576988633815 3.64939506315672 9 1.0137031393746396 3.5080723464517938 >10 4.468922924621979 24.152163711225015 >50 0.050461462588199404 1.2961867849968092 >100 0.024919240784296 1.7666563554521286 >500 0.0018689430588222002 0.5155043358553195 >1k 0.0049838481568592 4.006896387477662 >5k 0.0018689430588222002 6.478298912114898 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC 9771 2.329767905426349 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG 8686 2.0710637628219493 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC 8587 2.047458500040534 No Hit GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC 3110 0.7415390631333483 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2777 0.6621395428685878 No Hit GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT 2314 0.5517432128908578 No Hit GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT 2217 0.5286148241050268 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTA 1776 0.4234641080787223 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCTGAAT 1712 0.40820414022002965 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTC 1678 0.40009728229509917 No Hit CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC 1143 0.2725334884763399 No Hit CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT 991 0.23629106481194473 No Hit GAATGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT 649 0.15474561156705566 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC 512 0.12207974286954158 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATG 500 0.1192184988960367 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA 430 0.10252790905059157 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGT 423 0.10085885006604704 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.243682611743499 0.0 2 0.0 0.0 0.0 1.4301451127568563 0.0 3 0.0 0.0 0.0 1.7486969418070664 0.0 4 0.0 0.0 0.0 2.7079289839245777 0.0 5 0.0 0.0 0.0 6.077759073719951 0.0 6 0.0 0.0 0.0 7.0248308289500665 0.0 7 0.0 0.0 0.0 8.22464580183978 0.0 8 0.0 0.0 0.0 9.546540517599034 0.0 9 0.0 0.0 0.0 9.913256620203244 0.0 10 0.0 0.0 0.0 12.75781000386268 0.0 11 0.0 0.0 0.0 13.94927968182967 0.0 12 0.0 0.0 0.0 17.021778835378328 0.0 13 0.0 0.0 0.0 17.54753241550985 0.0 14 0.0 0.0 0.0 17.814581853036973 0.0 15 0.0 0.0 0.0 18.52727003943748 0.0 16 0.0 0.0 0.0 19.153405595639462 0.0 17 0.0 0.0 0.0 19.75212089709536 0.0 18 0.0 0.0 0.0 20.345829021597623 0.0 19 0.0 0.0 0.0 21.245451814267117 0.0 20 0.0 0.0 0.0 21.761667914486956 0.0 21 0.0 0.0 0.0 22.268584971792905 0.0 22 0.0 0.0 0.0 22.87564556817152 0.0 23 0.0 0.0 0.0 23.35633455572034 0.0 24 0.0 0.0 0.0 23.765969317927123 0.0 25 0.0 0.0 0.0 24.127916680575492 0.0 26 0.0 0.0 0.0 24.474604075365168 0.0 27 0.0 0.0 0.0 24.929780304150235 0.0 28 0.0 0.0 0.0 25.282667060882503 0.0 29 0.0 0.0 0.0 25.645091297526456 0.0 30 0.0 0.0 0.0 26.058064177702327 0.0 31 0.0 0.0 0.0 26.41858091836394 0.0 32 0.0 0.0 0.0 26.78315108798802 0.0 33 0.0 0.0 0.0 27.146052198627558 0.0 34 0.0 0.0 0.0 27.51109924224722 0.0 35 0.0 0.0 0.0 27.892360001716746 0.0 36 0.0 0.0 0.0 28.244054573460055 0.0 37 0.0 0.0 0.0 28.616493164011274 0.0 38 0.0 0.0 0.0 29.02302824524676 0.0 39 0.0 0.0 0.0 29.600761090896953 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAC 25 3.886554E-5 45.0 25 AACCGTA 20 7.028156E-4 45.0 40 TACGCGG 25 3.886554E-5 45.0 2 TCTACGG 20 7.028156E-4 45.0 2 CGGATGG 25 3.886554E-5 45.0 2 CGAATGC 110 0.0 45.0 45 CGTACGA 20 7.028156E-4 45.0 23 GCTACGA 25 3.886554E-5 45.0 10 TTACCGG 20 7.028156E-4 45.0 2 CAATCGA 50 2.1827873E-11 45.0 41 CCGTTAG 20 7.028156E-4 45.0 1 GCGAACC 30 2.1621927E-6 44.999996 33 ATCGAAT 145 0.0 43.448273 43 CCGATGA 1240 0.0 42.64113 18 ACGGGCG 80 0.0 42.1875 5 CGGGAGT 80 0.0 42.1875 6 CGTTTTT 1830 0.0 41.92623 1 CGATGAA 1285 0.0 41.673153 19 CCGATCG 60 3.6379788E-12 41.249996 40 CGATCGA 60 3.6379788E-12 41.249996 41 >>END_MODULE