FastQCFastQC Report
Sat 18 Jun 2016
SRR3551724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770464
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC131891.7118256011961623No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG104541.3568447065664326No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC98361.2766333014910496No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60020.7790110894214396No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC48550.6301397599368692No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT39090.5073566058894381No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT25650.33291626863811935No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT23350.30306412759064666No Hit
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC22490.29190202267724386No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTTCGTT21300.27645678448311667No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT20470.26568405532250694Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC19650.255041118079495No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTA17020.22090584375129793No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCC13510.17534888067450263No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG12790.16600386260746772No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC9280.12044689953067242No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT9040.11733189350832746No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT7940.10305478257257965No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.031836E-445.0000041
TAACGAG207.031836E-445.0000041
CTACCCG207.031836E-445.00000427
TCGTCGA253.8896047E-545.038
GCGATTC453.8380676E-1045.09
CCGACGA253.8896047E-545.018
CGTTTTT44400.043.2770271
CCGATCG850.042.35294340
TACGGCT11700.041.1538477
CCGATGA7750.040.3548418
CGATCGA950.040.26315741
ACTTGCG451.9268555E-840.01
TCGAATG900.040.044
ATCGAAT850.039.70588343
TAGAACG403.4562436E-739.3750041
AAGCGCG403.4562436E-739.3750041
CGGCTGT12250.039.3061269
CGATGAA8000.039.0937519
TGATACC13700.039.087594
ACGGCTG12400.038.8306438
Produced by FastQC (version 0.11.5)