##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551724_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	770464
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.321135056277775	31.0	31.0	33.0	28.0	34.0
2	31.276286757071063	31.0	31.0	34.0	28.0	34.0
3	31.1543212921045	31.0	31.0	34.0	28.0	34.0
4	34.944676195123975	35.0	35.0	37.0	32.0	37.0
5	35.28707376334261	35.0	35.0	37.0	33.0	37.0
6	35.2186851040412	36.0	35.0	37.0	33.0	37.0
7	35.540674191136766	37.0	35.0	37.0	33.0	37.0
8	35.16209972172613	37.0	35.0	37.0	32.0	37.0
9	37.08262813058105	39.0	37.0	39.0	33.0	39.0
10	36.57532993105453	38.0	35.0	39.0	32.0	39.0
11	36.79461726959339	39.0	37.0	39.0	33.0	39.0
12	36.92949573244175	39.0	37.0	39.0	33.0	39.0
13	36.97825855588321	39.0	37.0	39.0	33.0	39.0
14	38.02630752377788	40.0	37.0	41.0	33.0	41.0
15	38.05301221082361	40.0	37.0	41.0	33.0	41.0
16	38.14119932923537	40.0	37.0	41.0	34.0	41.0
17	37.940785033434395	40.0	37.0	41.0	33.0	41.0
18	37.85571421896416	39.0	37.0	41.0	33.0	41.0
19	37.74894608962911	39.0	37.0	41.0	33.0	41.0
20	37.6567198467417	39.0	36.0	41.0	33.0	41.0
21	37.50865582298459	39.0	36.0	41.0	33.0	41.0
22	37.684952184657554	39.0	36.0	41.0	33.0	41.0
23	37.736798864061136	39.0	36.0	41.0	33.0	41.0
24	37.61288522241143	39.0	36.0	41.0	33.0	41.0
25	37.27369740831499	39.0	35.0	41.0	32.0	41.0
26	37.43060415541803	39.0	35.0	41.0	33.0	41.0
27	37.49621007600615	39.0	36.0	41.0	33.0	41.0
28	37.38557544544586	39.0	35.0	41.0	33.0	41.0
29	37.419740571915106	39.0	35.0	41.0	33.0	41.0
30	37.06199640735972	39.0	35.0	41.0	31.0	41.0
31	37.08216217759688	39.0	35.0	41.0	32.0	41.0
32	36.91948747767579	39.0	35.0	41.0	31.0	41.0
33	36.79555177140009	39.0	35.0	41.0	31.0	41.0
34	36.62760103002866	39.0	35.0	41.0	30.0	41.0
35	36.48912733106284	39.0	35.0	41.0	30.0	41.0
36	36.26427062133987	39.0	35.0	41.0	30.0	41.0
37	36.251088954188646	39.0	35.0	41.0	30.0	41.0
38	36.13065762968808	39.0	35.0	41.0	29.0	41.0
39	36.08762252357021	39.0	35.0	41.0	29.0	41.0
40	36.05644780288242	39.0	35.0	41.0	29.0	41.0
41	35.890820336835986	39.0	35.0	41.0	27.0	41.0
42	36.00865063130789	39.0	35.0	41.0	29.0	41.0
43	36.002585454998545	39.0	35.0	41.0	29.0	41.0
44	36.109431459484156	39.0	35.0	41.0	29.0	41.0
45	36.08322776924035	39.0	35.0	41.0	29.0	41.0
46	35.97822221414628	39.0	35.0	41.0	28.0	41.0
47	35.77098605515637	39.0	35.0	41.0	27.0	41.0
48	35.71826847198571	39.0	35.0	40.0	27.0	41.0
49	35.82873177721477	39.0	35.0	41.0	28.0	41.0
50	35.74300551356066	39.0	35.0	41.0	28.0	41.0
51	34.89354077542883	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	14.0
9	22.0
10	37.0
11	20.0
12	22.0
13	22.0
14	31.0
15	46.0
16	60.0
17	134.0
18	253.0
19	580.0
20	1147.0
21	2047.0
22	2970.0
23	3993.0
24	5433.0
25	7712.0
26	10195.0
27	10840.0
28	10809.0
29	11331.0
30	12880.0
31	15933.0
32	20530.0
33	27913.0
34	45336.0
35	51704.0
36	62153.0
37	96722.0
38	177499.0
39	192049.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.249911741496035	18.55115878224031	20.77228786808988	17.426641608173775
2	32.15646675250239	29.83072538106907	22.56614196120779	15.44666590522075
3	29.132964032063796	28.16510570253769	28.42858329526104	14.273346970137476
4	25.943846824770528	22.744216472151844	34.424061344851935	16.887875358225692
5	28.672202724591934	26.263654109731277	26.917545790588527	18.14659737508826
6	23.960756115795157	35.05290318561283	26.741547950326037	14.244792748265981
7	71.99778315404744	5.093294430369232	18.28339805623624	4.625524359347095
8	72.15625908543423	9.108018025501515	13.346502886572248	5.389220002492005
9	66.1348226523238	7.1063670723096735	14.889728786808988	11.869081488557546
10	36.0791678780579	27.89397038667608	21.94469825974997	14.082163475516051
11	26.32128172114466	22.978620675333307	31.738796361672968	18.961301241849068
12	22.986537982306764	21.160235909789428	34.09010674087303	21.763119367030775
13	23.160459151887693	22.22764464011297	36.770829006936076	17.841067201063254
14	19.81883644141712	27.915126469244505	31.49777277069402	20.76826431864435
15	17.631972421813348	25.63922519416871	37.08025293848902	19.64854944552893
16	20.573057274577398	26.701961415458737	30.576509739585493	22.14847157037837
17	20.911554595672218	27.327688250197284	31.812388378950867	19.94836877517963
18	20.397838185820493	25.905039041408813	31.888316650745523	21.80880612202517
19	20.298028201187858	27.76794243468871	29.941048511027123	21.992980853096316
20	22.839483739668566	27.176610458113554	32.60437866013208	17.379527142085806
21	22.021275491132617	29.10090542841716	31.49530672425967	17.382512356190556
22	20.844971341944593	23.646140507538316	32.83021659675209	22.678671553765003
23	20.798635627362213	27.3269094986917	32.215522075009346	19.658932798936746
24	21.44188437097645	25.548500643767913	31.070887153715166	21.938727831540476
25	19.866210491340283	30.707859160194378	28.829380736802758	20.59654961166258
26	20.70765149312622	26.111278398471573	31.41483573534909	21.76623437305312
27	22.860769614154588	26.206156290235494	30.07135959629522	20.861714499314697
28	19.961737342692196	26.556724259666904	32.4387641732774	21.0427742243635
29	23.36332391909291	23.347878680898784	31.967359928562527	21.32143747144578
30	21.398533870498817	25.935929517797067	33.631162520247535	19.034374091456577
31	24.492643394110562	23.62667171989866	29.420842505295507	22.459842380695267
32	24.485894214395483	25.220516467998504	31.80888399717573	18.484705320430287
33	21.516255139759938	23.1431968268472	32.78271275491132	22.557835278481537
34	23.15215246916144	23.48052602068364	30.921626448477802	22.44569506167712
35	23.259490385014743	23.06441313286539	32.0371879802301	21.63890850188977
36	24.117155376500392	25.196634755160524	31.424050961498523	19.262158906840554
37	20.980733687751794	24.087173443535324	34.810192299705115	20.121900569007767
38	22.573280516675666	24.852426589691408	30.993531170826934	21.580761722805995
39	22.383265149312624	23.65937928313328	32.03589006105412	21.921465506499977
40	22.89282821780122	23.39382501972837	32.392558250612616	21.32078851185779
41	20.839649873323086	24.883187274162065	30.407261079038083	23.869901773476762
42	23.305177140009135	24.01773476762055	31.968398263903307	20.708689828467
43	23.097639863770404	23.421989865847074	31.449490177347677	22.030880093034845
44	22.233874652157663	23.51634858994061	30.931750218050418	23.31802653985131
45	21.993759604601905	22.38534181999419	29.915090127507582	25.705808447896334
46	24.310934709473774	24.590246916144036	29.532074178676744	21.566744195705446
47	19.70280246708477	23.86821447854799	34.79786206753333	21.631120986833906
48	21.75675956306849	23.97905677617643	30.421538189973834	23.842645470781243
49	21.783496698093614	21.794269427254225	34.41938883581842	22.00284503883374
50	21.401389292685966	22.181568509365786	32.7271618141795	23.68988038376874
51	20.89623914939569	21.558697096814385	30.353657017070233	27.191406736719692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	159.0
1	458.0
2	757.0
3	5744.5
4	10732.0
5	7474.5
6	4217.0
7	4153.0
8	4089.0
9	4062.0
10	4035.0
11	3956.5
12	3878.0
13	3684.5
14	3491.0
15	3413.5
16	3336.0
17	3234.5
18	3133.0
19	3099.5
20	3066.0
21	3317.0
22	3568.0
23	3714.5
24	3861.0
25	4419.5
26	5954.0
27	6930.0
28	8525.5
29	10121.0
30	11634.0
31	13147.0
32	14865.0
33	16583.0
34	19055.5
35	21528.0
36	23573.0
37	25618.0
38	27644.0
39	29670.0
40	33079.0
41	36488.0
42	38481.0
43	40474.0
44	44363.0
45	48252.0
46	77680.0
47	107108.0
48	92007.0
49	76906.0
50	74846.5
51	72787.0
52	61279.5
53	49772.0
54	44361.0
55	38950.0
56	34938.5
57	30927.0
58	27691.5
59	24456.0
60	22323.5
61	20191.0
62	17812.5
63	15434.0
64	13229.0
65	11024.0
66	9104.0
67	7184.0
68	5958.0
69	4732.0
70	3986.0
71	3240.0
72	2719.5
73	2199.0
74	1881.0
75	1245.0
76	927.0
77	694.5
78	462.0
79	346.5
80	231.0
81	201.0
82	171.0
83	114.0
84	57.0
85	41.5
86	26.0
87	16.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	770464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.50171710105743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64106674392187	26.88025385306724
2	8.830098026800897	6.446274802977834
3	3.450417841944061	3.778385278412496
4	2.2034839249122338	3.2172378745549617
5	1.613278536845245	2.9443718378566497
6	1.348779045771664	2.953965069635488
7	1.1838978501788622	3.0250013082645233
8	1.0304422306132737	3.009032863266264
9	0.8890971483508487	2.9208215326013613
>10	5.721113705987711	32.45467214217436
>50	0.05601094270054443	1.3918296558101204
>100	0.02441502371285441	1.7135754445900435
>500	0.0025133112645584314	0.63208981791645
>1k	0.0039494891300203925	3.458211491885003
>5k	7.180889327309804E-4	2.0756870280242037
>10k+	7.180889327309804E-4	3.0985899989630163
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	13189	1.7118256011961623	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG	10454	1.3568447065664326	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC	9836	1.2766333014910496	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6002	0.7790110894214396	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	4855	0.6301397599368692	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	3909	0.5073566058894381	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	2565	0.33291626863811935	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT	2335	0.30306412759064666	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	2249	0.29190202267724386	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTTCGTT	2130	0.27645678448311667	No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	2047	0.26568405532250694	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC	1965	0.255041118079495	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTA	1702	0.22090584375129793	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCC	1351	0.17534888067450263	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	1279	0.16600386260746772	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	928	0.12044689953067242	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT	904	0.11733189350832746	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	794	0.10305478257257965	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2979191759770735E-4	0.0	0.0	0.27645678448311667	0.0
2	1.2979191759770735E-4	0.0	0.0	1.3874755991194916	0.0
3	1.2979191759770735E-4	0.0	0.0	1.8120249615815924	0.0
4	1.2979191759770735E-4	0.0	0.0	2.772355359887029	0.0
5	1.2979191759770735E-4	0.0	0.0	5.630243593470947	0.0
6	1.2979191759770735E-4	0.0	0.0	6.913755866594675	0.0
7	1.2979191759770735E-4	0.0	0.0	8.063452672675167	0.0
8	1.2979191759770735E-4	0.0	0.0	9.405111724882667	0.0
9	1.2979191759770735E-4	0.0	0.0	9.913247082277692	0.0
10	1.2979191759770735E-4	0.0	0.0	12.170588113137018	0.0
11	1.2979191759770735E-4	0.0	0.0	13.403611330315238	0.0
12	1.2979191759770735E-4	0.0	0.0	15.673931552934336	0.0
13	1.2979191759770735E-4	0.0	0.0	16.15766602982099	0.0
14	1.2979191759770735E-4	0.0	0.0	16.40284296216306	0.0
15	1.2979191759770735E-4	0.0	0.0	16.91136769531088	0.0
16	1.2979191759770735E-4	0.0	0.0	17.487254433691906	0.0
17	1.2979191759770735E-4	0.0	0.0	18.184237031191593	0.0
18	1.2979191759770735E-4	0.0	0.0	18.865904182414752	0.0
19	1.2979191759770735E-4	0.0	0.0	19.658283839348755	0.0
20	1.2979191759770735E-4	0.0	0.0	20.170572538106907	0.0
21	1.2979191759770735E-4	0.0	0.0	20.75126157743905	0.0
22	1.2979191759770735E-4	0.0	0.0	21.401519084603564	0.0
23	1.2979191759770735E-4	0.0	0.0	21.99895128130581	0.0
24	1.2979191759770735E-4	0.0	0.0	22.488396602566766	0.0
25	1.2979191759770735E-4	0.0	0.0	22.92527619720065	0.0
26	1.2979191759770735E-4	0.0	0.0	23.353978901025876	0.0
27	1.2979191759770735E-4	0.0	0.0	23.814350832744942	0.0
28	1.2979191759770735E-4	0.0	0.0	24.237212900278273	0.0
29	1.2979191759770735E-4	0.0	0.0	24.6997912945965	0.0
30	1.2979191759770735E-4	0.0	0.0	25.2094841550027	0.0
31	1.2979191759770735E-4	0.0	0.0	25.674009428084894	0.0
32	2.595838351954147E-4	0.0	0.0	26.117897786269054	0.0
33	2.595838351954147E-4	0.0	0.0	26.56139676870042	0.0
34	2.595838351954147E-4	0.0	0.0	27.00242970469743	0.0
35	2.595838351954147E-4	0.0	0.0	27.44099659426008	0.0
36	2.595838351954147E-4	0.0	0.0	27.868660962744528	0.0
37	2.595838351954147E-4	0.0	0.0	28.31358765626947	0.0
38	2.595838351954147E-4	0.0	0.0	28.77136374963658	0.0
39	2.595838351954147E-4	0.0	0.0	29.24834904680816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.031836E-4	45.000004	1
TAACGAG	20	7.031836E-4	45.000004	1
CTACCCG	20	7.031836E-4	45.000004	27
TCGTCGA	25	3.8896047E-5	45.0	38
GCGATTC	45	3.8380676E-10	45.0	9
CCGACGA	25	3.8896047E-5	45.0	18
CGTTTTT	4440	0.0	43.277027	1
CCGATCG	85	0.0	42.352943	40
TACGGCT	1170	0.0	41.153847	7
CCGATGA	775	0.0	40.35484	18
CGATCGA	95	0.0	40.263157	41
ACTTGCG	45	1.9268555E-8	40.0	1
TCGAATG	90	0.0	40.0	44
ATCGAAT	85	0.0	39.705883	43
TAGAACG	40	3.4562436E-7	39.375004	1
AAGCGCG	40	3.4562436E-7	39.375004	1
CGGCTGT	1225	0.0	39.306126	9
CGATGAA	800	0.0	39.09375	19
TGATACC	1370	0.0	39.08759	4
ACGGCTG	1240	0.0	38.830643	8
>>END_MODULE