FastQCFastQC Report
Sat 18 Jun 2016
SRR3551722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547927
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCG52290.9543242074217915No Hit
GAATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC50880.9285908524310721No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC49940.911435282437259No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.4500599532419465No Hit
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC20750.3787000823102348Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT11570.21115951577491163No Hit
GAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT11170.2038592732243529No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC11060.20185170652294923Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTC8910.16261290281369598No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTA8760.15987531185723647No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATATCGCT8090.14764740558505057No Hit
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT7320.133594438675225Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC5610.10238590177158635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCACG302.1634132E-645.0000041
TACGAAT351.2103737E-745.00000412
TTACGCG253.888124E-545.01
ACGTAGG406.8030204E-945.02
GCTACGA253.888124E-545.010
TCGACGG207.030052E-445.02
AAGTGCG207.030052E-445.01
CTTACGA207.030052E-445.029
CGTAAGG850.042.352942
TACGGCT6250.041.0399977
ATGACGG556.002665E-1140.909092
CGTTTTT13800.040.760871
GATACCT6050.040.5371935
CGATGAA4950.040.45454419
CCGATGA4950.040.018
TGATACC6200.039.5564544
AAATGCG403.453806E-739.3751
ACGGCTG6600.038.863648
AACGTAG356.242304E-638.571431
CTCGAAT356.242304E-638.5714343