##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547927
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394311651004603	31.0	31.0	33.0	28.0	34.0
2	31.362599397364978	31.0	31.0	34.0	28.0	34.0
3	31.20746559304433	31.0	31.0	34.0	28.0	34.0
4	35.039129300071	35.0	35.0	37.0	32.0	37.0
5	35.35092995964791	35.0	35.0	37.0	33.0	37.0
6	35.25891040229811	37.0	35.0	37.0	33.0	37.0
7	35.62834647681169	37.0	35.0	37.0	35.0	37.0
8	35.209819921266885	37.0	35.0	37.0	32.0	37.0
9	37.10196796288557	39.0	37.0	39.0	33.0	39.0
10	36.59646084241149	38.0	35.0	39.0	32.0	39.0
11	36.716051590814104	39.0	35.0	39.0	33.0	39.0
12	36.6186152534918	39.0	35.0	39.0	33.0	39.0
13	36.62493361341931	39.0	35.0	39.0	33.0	39.0
14	37.56416091924654	39.0	36.0	41.0	33.0	41.0
15	37.64898973768403	39.0	36.0	41.0	33.0	41.0
16	37.71069686290327	39.0	36.0	41.0	33.0	41.0
17	37.466445347646676	39.0	35.0	41.0	32.0	41.0
18	37.458657813905866	39.0	36.0	41.0	33.0	41.0
19	37.409742538695845	39.0	35.0	41.0	33.0	41.0
20	37.41236697589277	39.0	35.0	41.0	32.0	41.0
21	37.23527769210132	39.0	35.0	41.0	32.0	41.0
22	37.415272472427894	39.0	35.0	41.0	33.0	41.0
23	37.492556490189386	39.0	35.0	41.0	33.0	41.0
24	37.398089526524515	39.0	35.0	41.0	33.0	41.0
25	37.049557696554466	39.0	35.0	41.0	32.0	41.0
26	37.15828933416313	39.0	35.0	41.0	32.0	41.0
27	37.243839051552484	39.0	35.0	41.0	33.0	41.0
28	37.1415352775096	39.0	35.0	41.0	32.0	41.0
29	37.20768825044212	39.0	35.0	41.0	33.0	41.0
30	36.88352097998456	39.0	35.0	41.0	32.0	41.0
31	36.92837914539711	39.0	35.0	41.0	32.0	41.0
32	36.83611137980059	39.0	35.0	41.0	32.0	41.0
33	36.83103588616732	39.0	35.0	41.0	32.0	41.0
34	36.642649477028876	39.0	35.0	41.0	31.0	41.0
35	36.617532992533675	39.0	35.0	41.0	31.0	41.0
36	36.509018537140896	39.0	35.0	41.0	31.0	41.0
37	36.572848207881705	39.0	35.0	41.0	31.0	41.0
38	36.43570767638755	39.0	35.0	41.0	31.0	41.0
39	36.410293707008414	39.0	35.0	41.0	31.0	41.0
40	36.30675984209575	39.0	35.0	41.0	30.0	41.0
41	36.2083854236057	38.0	35.0	40.0	30.0	41.0
42	36.29404281957268	39.0	35.0	41.0	31.0	41.0
43	36.27836372363472	39.0	35.0	41.0	31.0	41.0
44	36.3470553559142	39.0	35.0	41.0	31.0	41.0
45	36.27082257307999	39.0	35.0	41.0	31.0	41.0
46	36.14829347705078	38.0	35.0	40.0	31.0	41.0
47	35.9478707930071	38.0	35.0	40.0	30.0	41.0
48	35.90475373544286	38.0	35.0	40.0	30.0	41.0
49	36.00395125628049	38.0	35.0	40.0	30.0	41.0
50	35.92275613357254	38.0	35.0	40.0	30.0	41.0
51	35.04356237235982	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	30.0
10	34.0
11	32.0
12	27.0
13	29.0
14	22.0
15	44.0
16	78.0
17	104.0
18	204.0
19	364.0
20	646.0
21	1272.0
22	1825.0
23	2471.0
24	3162.0
25	4072.0
26	5068.0
27	5698.0
28	6443.0
29	7436.0
30	9432.0
31	11793.0
32	15927.0
33	23945.0
34	48914.0
35	46184.0
36	41896.0
37	63770.0
38	115925.0
39	131051.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1162782633453	18.791919361520787	21.983767910688833	15.10803446444508
2	31.64618644454462	28.02563115159501	23.398737423050882	16.92944498080949
3	31.391225473466356	27.352183776305967	25.47583893474861	15.78075181547907
4	28.668235002107945	24.000824927408214	29.172681762351555	18.15825830813229
5	28.50160696589144	27.725408676703285	24.062146964832905	19.71083739257237
6	25.81895033462487	34.92125775878904	24.87302140613622	14.386770500449877
7	74.34147249542366	6.453231908630164	14.076327685987366	5.128967909958808
8	75.05306363803938	9.070733875133001	10.513444309187173	5.362758177640452
9	69.01558054266353	7.792826416657693	12.233381454098813	10.958211586579965
10	45.59293482525957	21.23914317053184	18.842656047247168	14.32526595696142
11	37.13250122735327	21.938871419002897	23.154726815798455	17.773900537845368
12	32.7251622935172	20.001934564275896	26.74662865673712	20.52627448546978
13	28.67115509912817	20.77977540803793	29.665265628450506	20.883803864383392
14	22.621809109607664	24.98763521417999	30.00490941311525	22.385646263097094
15	22.770369045511536	22.60501855174138	34.52175198520971	20.10286041753737
16	27.314405021106825	23.764114562706347	28.372575178810315	20.54890523737651
17	25.7377351362499	25.480401586342705	29.05624289367014	19.72562038373725
18	26.248387102661486	25.182551690279908	28.28497226820361	20.284088938854993
19	24.43007918938107	28.922100936803623	25.771498758046235	20.876321115769073
20	27.9431384107737	27.569548498248853	28.01030064223884	16.47701244873861
21	25.930826551712183	29.959465403238024	26.870732780096617	17.238975264953176
22	23.423740753786547	24.30451501751146	31.05176419486537	21.219980033836624
23	25.70908168423896	25.12798237721448	29.903801053790012	19.259134884756545
24	27.553487964637625	22.639512197792772	27.9026220646181	21.904377772951506
25	24.175118218302803	26.541491841066417	25.394806242437408	23.888583698193372
26	21.884484612001234	24.741799546289926	30.008377028326766	23.365338813382074
27	22.84994168931263	25.67020789265723	27.804798814440613	23.675051603589527
28	19.502415467753917	27.937115710669485	29.57711519965251	22.98335362192409
29	26.253862284574403	25.98941099818041	26.401509690159457	21.355217027085725
30	24.080397571209303	27.308747333130146	28.91370565787048	19.69714943779007
31	26.95760566644827	24.307252608467916	27.120401075325727	21.614740649758087
32	29.00860881102775	26.76049911758318	25.93776178213521	18.293130289253863
33	26.712682528877025	24.337731121116498	26.980783936546292	21.968802413460185
34	24.359084330576884	24.68084252099276	29.51415060765394	21.445922540776415
35	23.27499831181891	29.045840048035597	26.83970674925674	20.83945489088875
36	22.010961314189665	31.40892856165146	26.04288527486326	20.53722484929562
37	23.370448983167467	31.235730307139452	25.898887990553487	19.494932719139594
38	22.72419501137925	28.806209586313503	25.622756316078604	22.84683908622864
39	24.533012609343945	27.210741576888893	25.53989856312976	22.716347250637405
40	24.78760856829468	23.39362725326549	27.437049095956212	24.381715082483616
41	20.99750514210835	26.25678238159463	24.87393393645504	27.871778539841984
42	21.89397492731696	25.038371899906377	26.810505779054512	26.257147393722157
43	21.952011855593902	24.191726270105324	26.21444097480139	27.641820899499386
44	21.102811140900158	24.162342793839326	27.736906558720413	26.997939506540103
45	23.008904470851043	23.571935677562887	26.901393798808964	26.5177660527771
46	23.324274949035182	27.53067470666713	25.991236058818057	23.153814285479633
47	20.277336214495726	24.90057982176458	30.52176658569481	24.300317378044888
48	21.190231545443098	24.27951168677579	29.673478401319887	24.856778366461228
49	23.196520704400402	22.34184480779374	30.874368300886797	23.58726618691906
50	20.68341220637056	22.567787314733533	29.838828895090042	26.90997158380587
51	19.91779926888071	22.01716652035764	29.76345389075552	28.30158032000613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	498.0
2	890.0
3	2966.0
4	5042.0
5	3280.5
6	1519.0
7	1465.0
8	1411.0
9	1422.0
10	1433.0
11	1407.5
12	1382.0
13	1353.0
14	1324.0
15	1317.5
16	1311.0
17	1350.5
18	1390.0
19	1394.0
20	1398.0
21	1700.0
22	2002.0
23	2088.0
24	2174.0
25	2569.0
26	3473.5
27	3983.0
28	5183.5
29	6384.0
30	6983.5
31	7583.0
32	8782.0
33	9981.0
34	10597.5
35	11214.0
36	12960.5
37	14707.0
38	15963.5
39	17220.0
40	18543.0
41	19866.0
42	21884.5
43	23903.0
44	26459.5
45	29016.0
46	42180.5
47	55345.0
48	50082.5
49	44820.0
50	45975.5
51	47131.0
52	43563.5
53	39996.0
54	37170.5
55	34345.0
56	32773.5
57	31202.0
58	31124.0
59	31046.0
60	30631.0
61	30216.0
62	27581.0
63	24946.0
64	20943.5
65	16941.0
66	13166.0
67	9391.0
68	7475.5
69	5560.0
70	4583.0
71	3606.0
72	2870.5
73	2135.0
74	1654.5
75	1027.0
76	880.0
77	695.5
78	511.0
79	392.5
80	274.0
81	202.0
82	130.0
83	77.0
84	24.0
85	31.5
86	39.0
87	21.0
88	3.0
89	5.0
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	547927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.07015969649792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.05100479702038	24.547892397276218
2	8.424132468864999	5.7402307703736755
3	3.4167243298118546	3.4922503066679913
4	2.169081400230021	2.956037988021605
5	1.7513102610948208	2.9833710136808005
6	1.4647065094558143	2.9941670811395777
7	1.2695192809470575	3.0276907247754616
8	1.1025251862518162	3.0050567332008393
9	0.9409145104680648	2.885139686915922
>10	7.32007253868014	40.54316268510876
>50	0.05659230720586995	1.3474727077890891
>100	0.026409743362737357	1.5009869056237932
>500	0.002694871771707894	0.7104622385439756
>1k	0.0032338461260494724	2.3715740012394533
>5k	0.0010779487086831575	1.8945047596428524
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCG	5229	0.9543242074217915	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC	5088	0.9285908524310721	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC	4994	0.911435282437259	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2466	0.4500599532419465	No Hit
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	2075	0.3787000823102348	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT	1157	0.21115951577491163	No Hit
GAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT	1117	0.2038592732243529	No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	1106	0.20185170652294923	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTC	891	0.16261290281369598	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTA	876	0.15987531185723647	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATATCGCT	809	0.14764740558505057	No Hit
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT	732	0.133594438675225	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC	561	0.10238590177158635	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1399821509069639	0.0
2	0.0	0.0	0.0	0.8373378205490878	0.0
3	0.0	0.0	0.0	1.0813484278015137	0.0
4	0.0	0.0	0.0	1.602585745911408	0.0
5	0.0	0.0	0.0	3.402095534624138	0.0
6	0.0	0.0	0.0	4.104196361924125	0.0
7	0.0	0.0	0.0	4.938249073325461	0.0
8	0.0	0.0	0.0	5.89476335351242	0.0
9	0.0	0.0	0.0	6.322557566975163	0.0
10	0.0	0.0	0.0	8.022966563064058	0.0
11	0.0	0.0	0.0	9.110520197033546	0.0
12	0.0	0.0	0.0	10.87663137607747	0.0
13	0.0	0.0	0.0	11.293840237841902	0.0
14	0.0	0.0	0.0	11.492589341280864	0.0
15	0.0	0.0	0.0	11.91874100016973	0.0
16	0.0	0.0	0.0	12.428662942326259	0.0
17	0.0	0.0	0.0	12.986218967125183	0.0
18	0.0	0.0	0.0	13.507821297362606	0.0
19	0.0	0.0	0.0	14.123049238310942	0.0
20	0.0	0.0	0.0	14.56617396112986	0.0
21	0.0	0.0	0.0	15.08321363977318	0.0
22	0.0	0.0	0.0	15.621606527876889	0.0
23	0.0	0.0	0.0	16.093202196642984	0.0
24	0.0	0.0	0.0	16.49216045203102	0.0
25	0.0	0.0	0.0	16.858997640196595	0.0
26	0.0	0.0	0.0	17.21743954942903	0.0
27	0.0	0.0	0.0	17.559820925050236	0.0
28	0.0	0.0	0.0	17.925563076833228	0.0
29	0.0	0.0	0.0	18.280537370854148	0.0
30	0.0	0.0	0.0	18.65686487433545	0.0
31	0.0	0.0	0.0	19.018591892715637	0.0
32	0.0	0.0	0.0	19.381048935350876	0.0
33	0.0	0.0	0.0	19.777817117973743	0.0
34	0.0	0.0	0.0	20.17038766113004	0.0
35	0.0	0.0	0.0	20.550000273759096	0.0
36	0.0	0.0	0.0	20.940745756277753	0.0
37	0.0	0.0	0.0	21.34353663900483	0.0
38	0.0	0.0	0.0	21.878461911897023	0.0
39	0.0	0.0	0.0	22.38874886618108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCACG	30	2.1634132E-6	45.000004	1
TACGAAT	35	1.2103737E-7	45.000004	12
TTACGCG	25	3.888124E-5	45.0	1
ACGTAGG	40	6.8030204E-9	45.0	2
GCTACGA	25	3.888124E-5	45.0	10
TCGACGG	20	7.030052E-4	45.0	2
AAGTGCG	20	7.030052E-4	45.0	1
CTTACGA	20	7.030052E-4	45.0	29
CGTAAGG	85	0.0	42.35294	2
TACGGCT	625	0.0	41.039997	7
ATGACGG	55	6.002665E-11	40.90909	2
CGTTTTT	1380	0.0	40.76087	1
GATACCT	605	0.0	40.537193	5
CGATGAA	495	0.0	40.454544	19
CCGATGA	495	0.0	40.0	18
TGATACC	620	0.0	39.556454	4
AAATGCG	40	3.453806E-7	39.375	1
ACGGCTG	660	0.0	38.86364	8
AACGTAG	35	6.242304E-6	38.57143	1
CTCGAAT	35	6.242304E-6	38.57143	43
>>END_MODULE