FastQCFastQC Report
Sat 18 Jun 2016
SRR3551721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527163
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC88241.6738655785781629No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTC85141.6150602375356389No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCG79781.51338390592663No Hit
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC32320.6130931040304423No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.557512572012831No Hit
GAACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCT18930.359091969656444No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGCATCAT18260.3463824282053179No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCT18220.34562364961122083No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTA17310.3283614365955122No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTC15520.2944060945096678No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT12170.23085838725403718No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC7990.15156602417089213No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCC7170.13601106299190194No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTC253.887923E-545.044
GTAGCGT253.887923E-545.014
CGGTCTA253.887923E-545.031
GTAAGCG207.0298085E-445.01
CCGTTCC253.887923E-545.045
TTGTGCG302.1632568E-644.9999961
TAGCGCG302.1632568E-644.9999961
TACGGCT8450.042.8698237
CGTTTTT17050.042.4926721
AACGAGC1300.041.5384615
TGATACC9550.040.9947664
GATGAAT7100.040.88028320
TTACGGG1000.040.53
CGATCAT501.0786607E-940.510
CGATGAA6950.040.46762519
ACGGCTG9000.040.2500048
TACGGGT451.9248546E-840.04
GATACCT9850.039.9746175
TCGTTCA403.4534787E-739.37516
GCGAACC403.4534787E-739.37533