FastQCFastQC Report
Sat 18 Jun 2016
SRR3551719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585124
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC137912.356936307517723No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC132382.2624264258516145No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG123932.1180125922026782No Hit
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC51530.8806680293407892RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43400.7417231219365468No Hit
GAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT31980.5465508165790499RNA PCR Primer, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT29760.5086101407564892No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACCATTT24600.4204237050608076No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTA23000.3930790738373405No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTC22320.3814576055673669No Hit
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT14250.24353812183400442Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC13390.2288403825513908Illumina PCR Primer Index 11 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT7910.13518502061101578No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC7720.13193784565322908No Hit
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC7280.12441807206677559RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATG7240.12373445628618891No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5860.10014971185594848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAG551.8189894E-1245.0000041
CTATGCG253.8884493E-545.01
CAGCGCG207.030444E-445.01
CGAACCA253.8884493E-545.015
GCGAACC253.8884493E-545.033
TTTCGCG351.2105556E-745.01
CGGTTAG253.8884493E-545.02
TAGCACG253.8884493E-545.01
GTCAACG207.030444E-445.01
CGAATGT207.030444E-445.045
CGGTCTA207.030444E-445.031
ACCCGTC207.030444E-445.039
ACACTAC207.030444E-445.015
CCGAAAT253.8884493E-545.045
CCGATCG302.163668E-644.99999640
CGATCGA302.163668E-644.99999641
CGATGAA10900.043.76146719
CGTTTTT27800.043.5431631
CCGATGA11000.043.36363618
TACGGCT14750.042.406787
Produced by FastQC (version 0.11.5)