##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585124
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17667195329537	31.0	31.0	33.0	28.0	34.0
2	31.131946390850487	31.0	31.0	34.0	28.0	34.0
3	31.0167229510326	31.0	31.0	34.0	28.0	34.0
4	34.915816134699654	35.0	35.0	37.0	32.0	37.0
5	35.2040319658739	35.0	35.0	37.0	33.0	37.0
6	35.109809202835635	36.0	35.0	37.0	32.0	37.0
7	35.41510346524839	37.0	35.0	37.0	33.0	37.0
8	34.91538374771843	37.0	35.0	37.0	32.0	37.0
9	36.781316438908675	39.0	37.0	39.0	32.0	39.0
10	36.313695900356166	37.0	35.0	39.0	32.0	39.0
11	36.588776054306436	38.0	35.0	39.0	32.0	39.0
12	36.69742311031508	39.0	35.0	39.0	32.0	39.0
13	36.66458391725514	39.0	35.0	39.0	32.0	39.0
14	37.67317354953822	39.0	36.0	41.0	33.0	41.0
15	37.66754226454564	39.0	36.0	41.0	32.0	41.0
16	37.83875383679357	39.0	37.0	41.0	33.0	41.0
17	37.567443823873234	39.0	36.0	41.0	32.0	41.0
18	37.5242375975007	39.0	36.0	40.0	32.0	41.0
19	37.45970255877387	39.0	36.0	40.0	32.0	41.0
20	37.25986286667442	39.0	35.0	40.0	32.0	41.0
21	37.2667195329537	39.0	35.0	40.0	32.0	41.0
22	37.4216918123338	39.0	35.0	41.0	32.0	41.0
23	37.402425810597414	39.0	35.0	41.0	32.0	41.0
24	37.27258153827223	39.0	35.0	41.0	32.0	41.0
25	37.00983723108264	39.0	35.0	40.0	32.0	41.0
26	37.098763680860806	39.0	35.0	40.0	32.0	41.0
27	37.115819894586444	39.0	35.0	40.0	32.0	41.0
28	36.96979614577423	39.0	35.0	41.0	31.0	41.0
29	37.07285635181603	39.0	35.0	41.0	31.0	41.0
30	36.75388293763373	39.0	35.0	40.0	31.0	41.0
31	36.751823545094716	39.0	35.0	40.0	31.0	41.0
32	36.56315071677115	39.0	35.0	40.0	30.0	41.0
33	36.45749618884202	39.0	35.0	41.0	30.0	41.0
34	36.282263246764785	39.0	35.0	41.0	30.0	41.0
35	36.23953384239922	39.0	35.0	41.0	30.0	41.0
36	36.0732938659156	39.0	35.0	41.0	29.0	41.0
37	36.22634518495225	39.0	35.0	41.0	30.0	41.0
38	35.97145904116051	39.0	35.0	41.0	28.0	41.0
39	35.93009515931666	39.0	35.0	40.0	28.0	41.0
40	35.93466513080988	39.0	35.0	40.0	28.0	41.0
41	35.554412398055796	39.0	35.0	40.0	26.0	41.0
42	35.78595989909831	39.0	35.0	40.0	27.0	41.0
43	35.797702709169336	39.0	35.0	40.0	28.0	41.0
44	35.99035076325702	39.0	35.0	41.0	29.0	41.0
45	35.897279892809046	39.0	35.0	40.0	28.0	41.0
46	35.779697978548135	39.0	35.0	40.0	28.0	41.0
47	35.502466143928466	38.0	35.0	40.0	27.0	41.0
48	35.52926559156692	38.0	35.0	40.0	27.0	41.0
49	35.68995460791217	39.0	35.0	40.0	28.0	41.0
50	35.59454235341568	39.0	35.0	40.0	27.0	41.0
51	34.86588312904615	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	14.0
10	22.0
11	22.0
12	13.0
13	24.0
14	25.0
15	31.0
16	53.0
17	102.0
18	238.0
19	514.0
20	1052.0
21	1973.0
22	3111.0
23	3935.0
24	5121.0
25	6428.0
26	7664.0
27	7695.0
28	7936.0
29	8721.0
30	10450.0
31	13507.0
32	17373.0
33	23710.0
34	38724.0
35	43761.0
36	48416.0
37	73791.0
38	131121.0
39	129553.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.13223180043889	17.955168477109126	20.123939541020363	14.788660181431629
2	29.3339531449744	32.90225661569172	21.882199328689303	15.88159091064458
3	26.74988549435675	32.22120439428224	26.799105830558993	14.22980428080202
4	24.68741668432674	22.01225723094592	36.60950499381328	16.690821090914064
5	31.048461522685788	24.323049473274043	25.4899816107355	19.138507393304664
6	22.231014280733657	37.62142725302671	26.297160943663222	13.85039752257641
7	68.39917692660016	5.0052638415105175	21.53235895297407	5.063200278915239
8	67.46638319398966	12.79797102836322	13.459027488190536	6.276618289456594
9	62.71576623074767	6.468030708020864	14.380541560421381	16.435661500810085
10	39.44052884516786	23.431956303279307	22.222298179531176	14.905216672021657
11	30.065080222311853	22.700829225941852	28.329721563292566	18.90436898845373
12	25.026490111497736	19.16875739159563	31.99356033934687	23.811192157559766
13	24.866011307005014	21.31804540576015	36.02501350141167	17.790929785823177
14	19.94038870393284	28.797485661159	29.033504009406553	22.228621625501603
15	16.543501890197636	24.29843930517292	38.149520443529916	21.008538361099525
16	19.268394391616138	25.50997737231766	29.41701246231568	25.804615773750523
17	19.08962886499272	27.677893916503173	31.750193121458015	21.482284097046094
18	19.21541416862067	25.45204093491294	31.45743466342177	23.87511023304462
19	19.65822629049569	27.318141111969428	28.087550673019734	24.936081924515147
20	22.363806646112618	26.994961751697076	33.48879895543509	17.152432646755216
21	21.01041830449614	31.25252083319091	29.924084467565848	17.812976394747096
22	19.312829417354273	23.504419576021494	32.557714262276036	24.625036744348204
23	21.12236038856721	28.46353935234241	30.979587232791683	19.4345130262987
24	22.26058066324403	24.642981658588607	29.142540726410125	23.953896951757233
25	18.064034290167555	32.942419042801184	26.727667981487684	22.265878685543576
26	18.259548403415344	24.39910172886431	31.601164881290124	25.740184986430226
27	22.73842809387412	26.222646823579275	27.489386865006395	23.549538217540213
28	15.91748073912538	28.46097579316521	31.33882732548998	24.282716142219428
29	20.150087844627805	22.247079251577443	29.10357462691669	28.499258276878063
30	18.638784257695804	27.85836848257805	28.41483172797561	25.088015531750536
31	20.127528523868445	28.185991345424217	24.95676130187789	26.729718828829448
32	22.468228956597233	26.483104435982803	25.49852680799284	25.55013979942713
33	19.58695934536953	24.680580526520874	25.66242369138849	30.070036436721104
34	19.022634518495224	27.370608623129456	26.986416554439742	26.620340303935574
35	18.046089375927153	23.37726704083237	29.492210198180214	29.084433385060258
36	16.871979272769533	29.884263848346677	27.888447576923863	25.355309301959927
37	16.858819668993238	26.56120753891483	29.792317525857765	26.787655266234168
38	17.796740519958163	27.729506907937463	26.06900417689242	28.404748395211953
39	20.25348473144154	26.440036641805843	27.36889958367799	25.93757904307463
40	19.185335074274853	22.833621591320814	31.609026462766863	26.372016871637467
41	17.033825308823427	27.996971582092	29.636965839719444	25.332237269365127
42	18.340727777360012	23.47622042507229	34.15327349416534	24.029778303402356
43	20.587431040258135	23.9325339586139	29.04102378299301	26.43901121813496
44	20.10411468338335	23.32855941646557	28.558391041898812	28.008934858252267
45	19.207894395034213	22.14863857917296	27.622691942220793	31.020775083572026
46	23.691046684121655	25.995173672589054	25.987995706892896	24.325783936396387
47	16.047709545327145	24.435675173125695	35.248425974665196	24.26818930688196
48	18.955640171997732	25.717796569616013	28.36185150498014	26.964711753406117
49	18.76268961792714	21.079976210170837	35.29183557673245	24.865498595169573
50	19.713770072668357	21.400933819156283	31.555533527935957	27.329762580239404
51	17.89791565548499	21.134494568672622	28.345615630191208	32.62197414565118
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	296.5
2	527.0
3	4162.0
4	7797.0
5	5256.0
6	2715.0
7	2665.5
8	2616.0
9	2519.0
10	2422.0
11	2372.5
12	2323.0
13	2212.5
14	2102.0
15	2017.5
16	1933.0
17	1897.0
18	1861.0
19	1867.5
20	1874.0
21	2004.5
22	2135.0
23	2199.0
24	2263.0
25	2554.5
26	3656.0
27	4466.0
28	5078.5
29	5691.0
30	6925.5
31	8160.0
32	9179.0
33	10198.0
34	12067.0
35	13936.0
36	15903.5
37	17871.0
38	18503.0
39	19135.0
40	21292.0
41	23449.0
42	25781.5
43	28114.0
44	30932.5
45	33751.0
46	64915.5
47	96080.0
48	77966.0
49	59852.0
50	58979.0
51	58106.0
52	49243.5
53	40381.0
54	35856.5
55	31332.0
56	27759.5
57	24187.0
58	22241.5
59	20296.0
60	18991.5
61	17687.0
62	16079.5
63	14472.0
64	11760.0
65	9048.0
66	7173.0
67	5298.0
68	4415.5
69	3533.0
70	2962.0
71	2391.0
72	2022.5
73	1654.0
74	1391.0
75	863.0
76	598.0
77	543.5
78	489.0
79	357.0
80	225.0
81	145.5
82	66.0
83	48.5
84	31.0
85	20.5
86	10.0
87	8.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	585124.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.80354800559667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83710571156492	27.54271012655492
2	9.58539174352281	7.055528503703836
3	3.5184921736959724	3.884789868658076
4	1.953928258894774	2.876459699029029
5	1.4091755878764085	2.5931330698362154
6	1.0779106911890466	2.3802562721353175
7	0.9086279003793262	2.340851138558453
8	0.7744103156860167	2.2800837783503667
9	0.6795649930067901	2.2509362558743526
>10	5.149146392220281	31.12479980461143
>50	0.0657049066096007	1.6409280719313923
>100	0.03122148044504293	2.0065696531447372
>500	0.003727937963587215	0.901927333836596
>1k	0.003727937963587215	3.476339048653318
>5k	4.6599224544840187E-4	0.8837481557824642
>10k+	0.0013979767363452057	6.760939219339472
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	13791	2.356936307517723	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	13238	2.2624264258516145	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	12393	2.1180125922026782	No Hit
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	5153	0.8806680293407892	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4340	0.7417231219365468	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	3198	0.5465508165790499	RNA PCR Primer, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	2976	0.5086101407564892	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACCATTT	2460	0.4204237050608076	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTA	2300	0.3930790738373405	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTC	2232	0.3814576055673669	No Hit
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	1425	0.24353812183400442	Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	1339	0.2288403825513908	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	791	0.13518502061101578	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	772	0.13193784565322908	No Hit
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	728	0.12441807206677559	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATG	724	0.12373445628618891	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	586	0.10014971185594848	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25362145459765795	0.0
2	0.0	0.0	0.0	1.632816291931283	0.0
3	0.0	0.0	0.0	2.032902427519637	0.0
4	0.0	0.0	0.0	3.1042992596441095	0.0
5	0.0	0.0	0.0	6.840943116330897	0.0
6	0.0	0.0	0.0	7.845174698012729	0.0
7	0.0	0.0	0.0	9.184207108236887	0.0
8	0.0	0.0	0.0	10.779766340126196	0.0
9	0.0	0.0	0.0	11.226167444849297	0.0
10	1.7090394514666978E-4	0.0	0.0	14.35798223966202	0.0
11	1.7090394514666978E-4	0.0	0.0	15.792549955223166	0.0
12	1.7090394514666978E-4	0.0	0.0	19.30257518064547	0.0
13	1.7090394514666978E-4	0.0	0.0	19.9039861636166	0.0
14	1.7090394514666978E-4	0.0	0.0	20.20375168340386	0.0
15	1.7090394514666978E-4	0.0	0.0	20.961539776184193	0.0
16	1.7090394514666978E-4	0.0	0.0	21.695230412698848	0.0
17	1.7090394514666978E-4	0.0	0.0	22.554535448896303	0.0
18	1.7090394514666978E-4	0.0	0.0	23.363252917330342	0.0
19	1.7090394514666978E-4	0.0	0.0	24.45960172544623	0.0
20	1.7090394514666978E-4	0.0	0.0	25.089553667256855	0.0
21	1.7090394514666978E-4	0.0	0.0	25.803419446134495	0.0
22	1.7090394514666978E-4	0.0	0.0	26.61811855264867	0.0
23	1.7090394514666978E-4	0.0	0.0	27.34787839842495	0.0
24	3.4180789029333955E-4	0.0	0.0	27.90929785823176	0.0
25	3.4180789029333955E-4	0.0	0.0	28.445765342047157	0.0
26	3.4180789029333955E-4	0.0	0.0	28.94531757371087	0.0
27	3.4180789029333955E-4	0.0	0.0	29.487083079825815	0.0
28	3.4180789029333955E-4	0.0	0.0	30.000991242881852	0.0
29	3.4180789029333955E-4	0.0	0.0	30.48123132874399	0.0
30	3.4180789029333955E-4	0.0	0.0	31.03444739918376	0.0
31	3.4180789029333955E-4	0.0	0.0	31.56988945932828	0.0
32	3.4180789029333955E-4	0.0	0.0	32.116098468017036	0.0
33	3.4180789029333955E-4	0.0	0.0	32.62368318510264	0.0
34	3.4180789029333955E-4	0.0	0.0	33.10050519206185	0.0
35	3.4180789029333955E-4	0.0	0.0	33.6115079880504	0.0
36	3.4180789029333955E-4	0.0	0.0	34.10490767768883	0.0
37	3.4180789029333955E-4	0.0	0.0	34.61779041707399	0.0
38	3.4180789029333955E-4	0.0	0.0	35.13938925766163	0.0
39	3.4180789029333955E-4	0.0	0.0	35.670900527067765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAAG	55	1.8189894E-12	45.000004	1
CTATGCG	25	3.8884493E-5	45.0	1
CAGCGCG	20	7.030444E-4	45.0	1
CGAACCA	25	3.8884493E-5	45.0	15
GCGAACC	25	3.8884493E-5	45.0	33
TTTCGCG	35	1.2105556E-7	45.0	1
CGGTTAG	25	3.8884493E-5	45.0	2
TAGCACG	25	3.8884493E-5	45.0	1
GTCAACG	20	7.030444E-4	45.0	1
CGAATGT	20	7.030444E-4	45.0	45
CGGTCTA	20	7.030444E-4	45.0	31
ACCCGTC	20	7.030444E-4	45.0	39
ACACTAC	20	7.030444E-4	45.0	15
CCGAAAT	25	3.8884493E-5	45.0	45
CCGATCG	30	2.163668E-6	44.999996	40
CGATCGA	30	2.163668E-6	44.999996	41
CGATGAA	1090	0.0	43.761467	19
CGTTTTT	2780	0.0	43.543163	1
CCGATGA	1100	0.0	43.363636	18
TACGGCT	1475	0.0	42.40678	7
>>END_MODULE