FastQCFastQC Report
Sat 18 Jun 2016
SRR3551717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382709
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG64991.6981570854095411No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC62871.6427625166902267No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC57351.4985276019116351No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.8852679189671525No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC21500.5617845412571953TruSeq Adapter, Index 20 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT14240.3720842729070913No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT12730.33262870745135337No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA10340.27017917007439074No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC10220.26704362844876917No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT9680.2529336911334722No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC9280.24248188571473367TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT5940.15520931046826703TruSeq Adapter, Index 23 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5780.15102858830077162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG302.1616925E-645.0000041
AGAACGG302.1616925E-645.0000042
TACTAGA302.1616925E-645.00000430
TACACGG351.2090823E-745.0000042
CCGATCG207.027384E-445.040
CTATCGA207.027384E-445.041
GCGCGAG207.027384E-445.01
AGTTGCG207.027384E-445.01
ACTACGG207.027384E-445.02
GTGTACG207.027384E-445.01
GCTACGA502.1827873E-1145.010
CGCAATC207.027384E-445.022
TCGAATG502.1827873E-1145.044
TAATCGC207.027384E-445.018
CGTTTTT19300.043.0181351
TACGGCT6450.042.20937
ACGGCTG6650.040.9398468
ACGGGTC556.002665E-1140.9090925
CGAATAT556.002665E-1140.90909214
ACGGGTA501.0768417E-940.55
Produced by FastQC (version 0.11.5)