##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551717_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382709
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.606975534936467	33.0	31.0	33.0	30.0	34.0
2	31.55642799098009	31.0	31.0	34.0	30.0	34.0
3	31.439242348625196	31.0	31.0	34.0	28.0	34.0
4	35.22911402658417	37.0	35.0	37.0	33.0	37.0
5	35.53281213663645	37.0	35.0	37.0	33.0	37.0
6	35.49246032886606	37.0	35.0	37.0	33.0	37.0
7	35.69834260495573	37.0	35.0	37.0	35.0	37.0
8	35.1986783692048	37.0	35.0	37.0	32.0	37.0
9	37.16385034059821	39.0	37.0	39.0	34.0	39.0
10	36.813171365188694	38.0	35.0	39.0	33.0	39.0
11	37.113130341852425	39.0	37.0	39.0	34.0	39.0
12	37.18629559273495	39.0	37.0	39.0	34.0	39.0
13	37.166633133790945	39.0	37.0	39.0	34.0	39.0
14	38.24301492779109	40.0	38.0	41.0	34.0	41.0
15	38.29528963259291	40.0	38.0	41.0	34.0	41.0
16	38.44004713764244	40.0	38.0	41.0	34.0	41.0
17	38.239197405861894	40.0	37.0	41.0	34.0	41.0
18	38.18418432804037	40.0	37.0	41.0	34.0	41.0
19	38.09030621176925	40.0	37.0	41.0	34.0	41.0
20	37.94763384190077	40.0	36.0	41.0	34.0	41.0
21	37.89951372975289	39.0	36.0	41.0	34.0	41.0
22	38.01030286719152	40.0	36.0	41.0	34.0	41.0
23	38.061265870413294	40.0	36.0	41.0	34.0	41.0
24	37.943664768793	40.0	36.0	41.0	34.0	41.0
25	37.6374242570726	39.0	36.0	41.0	33.0	41.0
26	37.76225278213995	39.0	36.0	41.0	33.0	41.0
27	37.830662461556955	40.0	36.0	41.0	34.0	41.0
28	37.71982629099394	40.0	36.0	41.0	33.0	41.0
29	37.790485721527325	40.0	36.0	41.0	33.0	41.0
30	37.51218811159393	40.0	36.0	41.0	33.0	41.0
31	37.549205793435746	40.0	36.0	41.0	33.0	41.0
32	37.388551092344315	40.0	36.0	41.0	33.0	41.0
33	37.26260422409716	40.0	36.0	41.0	33.0	41.0
34	37.120950905257	40.0	36.0	41.0	32.0	41.0
35	37.075373194777235	40.0	36.0	41.0	32.0	41.0
36	36.91016673242594	40.0	35.0	41.0	31.0	41.0
37	36.9809254551108	40.0	36.0	41.0	32.0	41.0
38	36.84073800198062	40.0	35.0	41.0	31.0	41.0
39	36.70793213642742	40.0	35.0	41.0	31.0	41.0
40	36.67200928120321	39.0	35.0	41.0	31.0	41.0
41	36.450269003341965	39.0	35.0	41.0	30.0	41.0
42	36.566877183447474	39.0	35.0	41.0	31.0	41.0
43	36.55788079193329	39.0	35.0	41.0	31.0	41.0
44	36.65248530868101	39.0	35.0	41.0	31.0	41.0
45	36.60888037647403	39.0	35.0	41.0	31.0	41.0
46	36.52459963052868	39.0	35.0	41.0	31.0	41.0
47	36.2798366382813	39.0	35.0	41.0	30.0	41.0
48	36.25557015905035	39.0	35.0	41.0	30.0	41.0
49	36.38330951192682	39.0	35.0	41.0	31.0	41.0
50	36.31135144457016	39.0	35.0	41.0	31.0	41.0
51	35.55379936191728	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	21.0
10	19.0
11	24.0
12	14.0
13	25.0
14	30.0
15	24.0
16	39.0
17	47.0
18	92.0
19	192.0
20	315.0
21	753.0
22	1217.0
23	1706.0
24	2356.0
25	3042.0
26	4062.0
27	4461.0
28	4205.0
29	4317.0
30	4765.0
31	5923.0
32	7897.0
33	11715.0
34	22607.0
35	26172.0
36	28008.0
37	45685.0
38	91054.0
39	111894.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.196480877115505	19.12001024276931	19.728305318139892	15.955203561975287
2	31.646499037127423	30.7941543052293	20.78289248489061	16.77645417275267
3	29.521908290633352	29.393089788847398	25.963068545552886	15.121933374966359
4	27.267192566675984	22.88213760324424	32.888957406279964	16.961712423799806
5	29.816910498577247	26.158778602018767	25.286575439825036	18.73773545957895
6	23.853110326645048	36.86665325351638	25.521218471475716	13.759017948362857
7	69.55441340548563	6.636373850628023	18.978910869616342	4.830301874270006
8	69.21028771207367	11.672053701376242	13.422469813879475	5.69518877267062
9	63.484527408553234	8.22295791319253	14.510764053105623	13.78175062514861
10	39.76310983018429	22.473472011371566	22.581386902320038	15.182031256124104
11	31.301328163173586	23.135071294377713	26.838668544507705	18.724931997940995
12	26.31764604438359	21.138515164263186	30.285412676472202	22.25842611488102
13	24.53430674481133	22.56936732608849	34.597566297108244	18.29875963199193
14	20.157874520850044	29.62329080319512	28.64369534032385	21.57513933563099
15	18.074045815489054	24.76450775915905	37.02944012291323	20.13200630243867
16	19.96869684277088	25.80132685669791	30.50855872216227	23.721417578368946
17	20.199943037660468	26.971406473325686	31.291398948025783	21.537251540988063
18	20.375272073559806	26.217308712363703	30.896843293468407	22.510575920608087
19	21.922661865804045	27.18984920657732	28.754484477762475	22.133004449856156
20	23.882636676952984	26.955206174926644	31.78472416379024	17.37743298433013
21	22.623978009401398	29.631390952394636	29.821875106151147	17.922755932052816
22	20.472473863954075	24.272489019071934	31.31099608318592	23.944041033788075
23	21.784698034276694	27.447747505284692	30.66977782074631	20.0977766396923
24	22.02535085404315	24.887316472829223	29.98701363176722	23.100319041360407
25	19.612290277991896	31.03924914229872	28.200277495433866	21.14818308427552
26	19.244909317523234	26.445680660763138	31.506967434787263	22.802442586926357
27	22.807929784771197	27.23766621636805	28.447201398451565	21.50720260040919
28	18.033022479220502	27.09421518699586	31.964233921857076	22.908528411926554
29	21.53542247503978	23.892565892100787	31.84011873250956	22.731892900349873
30	19.515088487597627	26.501597819753393	30.035875822099822	23.947437870549166
31	21.61720785244141	24.49040916205263	27.660441745556025	26.231941239949936
32	24.544235959959135	26.126116710085206	28.44615621790969	20.883491112045967
33	20.410808211983518	24.428743510082075	27.274247535333636	27.886200742600774
34	20.270492724236952	24.528819546966492	32.45050416896388	22.750183559832667
35	20.677067955025883	22.957651897394626	31.137757408370327	25.227522739209164
36	19.00059836586022	25.637494806759182	31.34653222160963	24.015374605770965
37	21.237023430334798	24.444943808481117	31.2697114517819	23.048321309402183
38	19.96477741573885	25.010909071905807	27.425276123634408	27.59903738872093
39	23.418837811496463	23.674123158849152	30.889265734539823	22.017773295114566
40	20.232866224729495	22.848691825904275	33.210873013177114	23.707568936189116
41	18.59114888858114	24.965443718334296	32.78914266453101	23.65426472855355
42	19.549579445479466	23.82070972984696	34.482073847231185	22.147636977442392
43	22.261039066235703	23.66994243668166	30.3144164365092	23.754602060573436
44	21.490741006874675	23.35482050330669	29.855059588355644	25.299378901462994
45	20.376317254101682	22.561789767159905	29.18274720479529	27.879145773943126
46	23.488342317531075	25.746193583114064	28.318644191801077	22.44681990755378
47	18.731203081192238	24.326577112113903	35.04908429119775	21.89313551549611
48	20.488935457488587	24.11179251075883	30.18899477148434	25.210277260268242
49	19.895011614568773	22.452568400534087	34.17426817764934	23.478151807247805
50	20.20333987442156	22.141627189326616	31.94150124507132	25.713531691180506
51	19.766715703053755	21.696118983352886	29.8919022024567	28.64526311113666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	352.5
2	624.0
3	3161.5
4	5699.0
5	3849.5
6	2000.0
7	1924.0
8	1848.0
9	1781.5
10	1715.0
11	1724.0
12	1733.0
13	1645.5
14	1558.0
15	1448.0
16	1338.0
17	1380.5
18	1423.0
19	1320.0
20	1217.0
21	1416.5
22	1616.0
23	1674.5
24	1733.0
25	2128.0
26	3028.0
27	3533.0
28	4370.0
29	5207.0
30	6104.0
31	7001.0
32	7484.5
33	7968.0
34	8941.0
35	9914.0
36	10337.0
37	10760.0
38	11966.0
39	13172.0
40	14385.0
41	15598.0
42	17046.0
43	18494.0
44	20588.0
45	22682.0
46	37333.0
47	51984.0
48	44298.5
49	36613.0
50	35502.0
51	34391.0
52	29810.0
53	25229.0
54	22699.0
55	20169.0
56	18248.5
57	16328.0
58	15444.5
59	14561.0
60	13791.0
61	13021.0
62	11701.0
63	10381.0
64	8928.5
65	7476.0
66	6044.5
67	4613.0
68	3755.0
69	2897.0
70	2581.0
71	2265.0
72	1893.0
73	1521.0
74	1151.0
75	644.5
76	508.0
77	394.5
78	281.0
79	210.5
80	140.0
81	104.5
82	69.0
83	50.5
84	32.0
85	19.0
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.32751173286958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16291587533811	24.383379372462816
2	9.306018628897586	6.202928900817743
3	3.3727642151191666	3.3721751685476002
4	1.874314892470459	2.4986500627960564
5	1.3717905091707603	2.2859182144713825
6	1.059727472649243	2.119084786701712
7	0.946262486020787	2.2075601873662483
8	0.8307412064571527	2.2149229844143195
9	0.7600512645246846	2.279755569242591
>10	7.192927241438318	40.029520215249555
>50	0.07145028794965808	1.5980611772440072
>100	0.040828735971233196	2.4657819671148324
>500	0.0031406719977871683	0.80282490450051
>1k	0.004711007996680753	2.692917565910935
>5k	0.0023555039983403766	4.846518923159696
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	6499	1.6981570854095411	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	6287	1.6427625166902267	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	5735	1.4985276019116351	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3388	0.8852679189671525	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	2150	0.5617845412571953	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT	1424	0.3720842729070913	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT	1273	0.33262870745135337	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA	1034	0.27017917007439074	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC	1022	0.26704362844876917	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT	968	0.2529336911334722	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	928	0.24248188571473367	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	594	0.15520931046826703	TruSeq Adapter, Index 23 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	578	0.15102858830077162	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1601739180421678	0.0
2	0.0	0.0	0.0	1.11860447494049	0.0
3	0.0	0.0	0.0	1.464036644029798	0.0
4	0.0	0.0	0.0	2.143926586518739	0.0
5	0.0	0.0	0.0	4.816453232090178	0.0
6	0.0	0.0	0.0	5.63718125259662	0.0
7	0.0	0.0	0.0	6.671909989051734	0.0
8	0.0	0.0	0.0	7.851657525691844	0.0
9	0.0	0.0	0.0	8.213551288315665	0.0
10	0.0	0.0	0.0	10.695071189859659	0.0
11	0.0	0.0	0.0	11.892848090847092	0.0
12	0.0	0.0	0.0	14.517557726627803	0.0
13	0.0	0.0	0.0	15.058699952182991	0.0
14	0.0	0.0	0.0	15.286288015176021	0.0
15	0.0	0.0	0.0	15.848333851568686	0.0
16	0.0	0.0	0.0	16.528485089193094	0.0
17	0.0	0.0	0.0	17.222484968997332	0.0
18	0.0	0.0	0.0	17.937911049909985	0.0
19	0.0	0.0	0.0	18.798094635872165	0.0
20	0.0	0.0	0.0	19.33244318790517	0.0
21	0.0	0.0	0.0	19.89004700699487	0.0
22	0.0	0.0	0.0	20.51637144671278	0.0
23	0.0	0.0	0.0	21.091743335014332	0.0
24	0.0	0.0	0.0	21.590817043759095	0.0
25	0.0	0.0	0.0	22.02717991999143	0.0
26	0.0	0.0	0.0	22.436106806999575	0.0
27	0.0	0.0	0.0	22.88396666919252	0.0
28	0.0	0.0	0.0	23.299948524858312	0.0
29	0.0	0.0	0.0	23.706001165376303	0.0
30	0.0	0.0	0.0	24.16770966974908	0.0
31	0.0	0.0	0.0	24.58891742812424	0.0
32	0.0	0.0	0.0	25.017964040563456	0.0
33	0.0	0.0	0.0	25.432378125416438	0.0
34	0.0	0.0	0.0	25.851495522707854	0.0
35	0.0	0.0	0.0	26.258854638903188	0.0
36	0.0	0.0	0.0	26.71585983083753	0.0
37	0.0	0.0	0.0	27.15561954383095	0.0
38	2.61295135468463E-4	0.0	0.0	27.593288895740628	0.0
39	2.61295135468463E-4	0.0	0.0	28.02442586926359	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	30	2.1616925E-6	45.000004	1
AGAACGG	30	2.1616925E-6	45.000004	2
TACTAGA	30	2.1616925E-6	45.000004	30
TACACGG	35	1.2090823E-7	45.000004	2
CCGATCG	20	7.027384E-4	45.0	40
CTATCGA	20	7.027384E-4	45.0	41
GCGCGAG	20	7.027384E-4	45.0	1
AGTTGCG	20	7.027384E-4	45.0	1
ACTACGG	20	7.027384E-4	45.0	2
GTGTACG	20	7.027384E-4	45.0	1
GCTACGA	50	2.1827873E-11	45.0	10
CGCAATC	20	7.027384E-4	45.0	22
TCGAATG	50	2.1827873E-11	45.0	44
TAATCGC	20	7.027384E-4	45.0	18
CGTTTTT	1930	0.0	43.018135	1
TACGGCT	645	0.0	42.2093	7
ACGGCTG	665	0.0	40.939846	8
ACGGGTC	55	6.002665E-11	40.909092	5
CGAATAT	55	6.002665E-11	40.909092	14
ACGGGTA	50	1.0768417E-9	40.5	5
>>END_MODULE