FastQCFastQC Report
Sat 18 Jun 2016
SRR3551715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569262
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC51030.896423790802829No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG47150.8282653681433154No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC45070.791726832284607No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39870.7003804926378363No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC32410.5693336284522769TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC14250.25032410383970827TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT12280.21571789439660474TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT11720.20588059628079863No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT9600.16863939627096133No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT9350.16424774532640507No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC8800.15458611324838123No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTA8770.15405911513503448No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC7100.12472288682539849TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC6260.10996693965168937No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAC207.030281E-445.00000426
CCGTCGA207.030281E-445.00000441
TATTGCG207.030281E-445.0000041
CCATCGC207.030281E-445.00000429
CGGATCA207.030281E-445.00000442
CGTCGAA207.030281E-445.00000442
GCAATCG207.030281E-445.00000421
AATAGCG207.030281E-445.0000041
ATCGGGT207.030281E-445.0000044
CGCAATC207.030281E-445.00000420
GCGCTAG302.1635642E-645.0000041
GTTGCGT207.030281E-445.00000427
TGCGACT253.888317E-545.021
CGTTTTT22900.042.936681
CGAGGGT750.042.04
TCGAAGG650.041.538462
ACGGCTG5500.040.9090928
TACGGCT5550.040.540547
GCGAGAC451.9254003E-840.021
CACGACC451.9254003E-840.027