##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551715_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 569262 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31052309832731 31.0 31.0 33.0 28.0 34.0 2 31.27544434724257 31.0 31.0 34.0 28.0 34.0 3 31.116229433898628 31.0 31.0 34.0 28.0 34.0 4 34.96119712891428 35.0 35.0 37.0 32.0 37.0 5 35.31683829238558 35.0 35.0 37.0 33.0 37.0 6 35.23271358355204 37.0 35.0 37.0 33.0 37.0 7 35.548051687974954 37.0 35.0 37.0 33.0 37.0 8 35.09651794779908 37.0 35.0 37.0 32.0 37.0 9 37.015137142475695 39.0 37.0 39.0 32.0 39.0 10 36.52608816327104 38.0 35.0 39.0 32.0 39.0 11 36.76914847644846 39.0 37.0 39.0 33.0 39.0 12 36.78408711630146 39.0 35.0 39.0 33.0 39.0 13 36.80078241653228 39.0 35.0 39.0 33.0 39.0 14 37.73870555210079 39.0 36.0 41.0 33.0 41.0 15 37.86581925370041 40.0 36.0 41.0 33.0 41.0 16 37.88880691140459 39.0 36.0 41.0 33.0 41.0 17 37.67496161697075 39.0 36.0 41.0 33.0 41.0 18 37.653410907455616 39.0 36.0 41.0 33.0 41.0 19 37.588180837645936 39.0 36.0 41.0 33.0 41.0 20 37.568276118904826 39.0 35.0 41.0 33.0 41.0 21 37.361840417944634 39.0 35.0 41.0 33.0 41.0 22 37.54660771314439 39.0 35.0 41.0 33.0 41.0 23 37.644794488302395 39.0 35.0 41.0 33.0 41.0 24 37.5158415632872 39.0 35.0 41.0 33.0 41.0 25 37.13873225333853 39.0 35.0 41.0 32.0 41.0 26 37.307798518081306 39.0 35.0 41.0 33.0 41.0 27 37.41641809922321 39.0 35.0 41.0 33.0 41.0 28 37.297704747550334 39.0 35.0 41.0 33.0 41.0 29 37.368410327757694 39.0 35.0 41.0 33.0 41.0 30 37.0436600370304 39.0 35.0 41.0 32.0 41.0 31 37.06047303350654 39.0 35.0 41.0 32.0 41.0 32 36.96398670559426 39.0 35.0 41.0 32.0 41.0 33 36.90591327016383 39.0 35.0 41.0 31.0 41.0 34 36.75021167757553 39.0 35.0 41.0 31.0 41.0 35 36.71388218430178 39.0 35.0 41.0 31.0 41.0 36 36.549534309333836 39.0 35.0 41.0 31.0 41.0 37 36.61676697197424 39.0 35.0 41.0 31.0 41.0 38 36.44541880540068 39.0 35.0 41.0 30.0 41.0 39 36.44298758743777 39.0 35.0 41.0 30.0 41.0 40 36.34708974075206 39.0 35.0 41.0 30.0 41.0 41 36.22071910649227 39.0 35.0 41.0 30.0 41.0 42 36.30126022815505 39.0 35.0 41.0 30.0 41.0 43 36.3033629506273 39.0 35.0 41.0 30.0 41.0 44 36.37021441796572 39.0 35.0 41.0 31.0 41.0 45 36.336173853164276 39.0 35.0 41.0 30.0 41.0 46 36.24356974468698 39.0 35.0 41.0 30.0 41.0 47 36.08089596705911 39.0 35.0 41.0 30.0 41.0 48 36.04059817799185 39.0 35.0 41.0 30.0 41.0 49 36.154315587550194 39.0 35.0 41.0 30.0 41.0 50 36.088716619061174 39.0 35.0 41.0 30.0 41.0 51 35.27186251673219 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 16.0 9 30.0 10 38.0 11 25.0 12 22.0 13 18.0 14 21.0 15 28.0 16 59.0 17 98.0 18 184.0 19 342.0 20 651.0 21 1124.0 22 1635.0 23 2264.0 24 3198.0 25 4728.0 26 6483.0 27 7023.0 28 7124.0 29 7900.0 30 9374.0 31 11745.0 32 15668.0 33 22533.0 34 42859.0 35 44952.0 36 43896.0 37 67388.0 38 125778.0 39 142046.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70806061180967 19.055373448429723 20.74299707340381 15.49356886635679 2 33.94851579764678 26.53857099191585 22.67953947391535 16.833373736522024 3 31.179843376160715 25.967832035161315 27.11229627131268 15.74002831736529 4 28.902333196313823 23.55505900622209 29.49889506062235 18.043712736841737 5 27.66880627900685 26.840013912750194 26.1976032125805 19.293576595662454 6 25.535869248254755 34.38504590153567 26.19690054842937 13.882184301780198 7 72.28464222098084 6.7199286093222454 15.616535092804368 5.378894076892538 8 71.65382547930479 8.95106295519462 13.14333294686805 6.251778618632545 9 65.75425726642565 8.111027962519895 15.328618456879259 10.806096314175196 10 41.197726178806946 21.693701669881353 22.617529362578214 14.491042788733482 11 33.05525399552403 22.74506290600813 26.181793269180098 18.017889829287746 12 29.28036650962123 20.61177454318047 30.41429078350566 19.69356816369264 13 25.721021252077254 21.97178100769066 32.399141344407326 19.90805639582477 14 21.014752433852955 26.279112254111464 31.763757285748916 20.942378026286665 15 21.65944679251382 24.382270378138713 35.47259434144559 18.48568848790188 16 24.18060576676469 24.167782146006584 30.762987868503433 20.888624218725298 17 22.646338592774505 25.95799473704551 30.49790781749001 20.897758852689975 18 23.478644279786813 25.155200944380617 31.259595757313857 20.106559018518713 19 23.120812560824366 28.265895141428725 28.497422979225735 20.115869318521174 20 25.490020412393587 26.979844078824865 30.631589672242303 16.89854583653924 21 23.571571613773624 28.97066728501112 30.16466934381708 17.293091757398173 22 21.440918241512698 24.285829723396258 31.9480309593825 22.325221075708548 23 23.324058166538432 25.55571951052415 30.86996848551282 20.250253837424594 24 25.061746612280462 22.812342998478734 30.128306473996158 21.99760391524465 25 20.999820820641464 27.027449575063855 28.155401203663693 23.817328400630995 26 19.10297894466871 25.220724376473395 32.05202525374959 23.624271425108297 27 21.491510060393985 25.797435978512528 30.242489398554618 22.468564562538866 28 18.036334763254878 26.554732267391817 32.50612196141671 22.90281100793659 29 22.168878302082344 24.13844591769695 29.924533870168744 23.768141910051963 30 21.536480566066242 24.811598174478537 32.68758497844578 20.964336281009448 31 22.978347404182962 24.201510025260774 29.65330550783295 23.166837062723317 32 24.121933310145415 24.81089551032741 29.078526232209423 21.988644947317756 33 23.394851579764676 23.579652251511607 30.572214551471905 22.45328161725181 34 20.434351845020394 23.591948874156362 31.392223615839455 24.581475664983788 35 19.227877497531892 25.269735201014647 31.3769406705524 24.125446630901063 36 20.997712828188074 27.035530212801838 30.506866785416907 21.45989017359318 37 20.828897765879333 25.929009840811435 31.54329640833219 21.69879598497704 38 20.580154656379662 26.09712223896905 30.101429570215473 23.221293534435812 39 23.005399974001424 24.33677287435311 28.379726733911625 24.27810041773384 40 21.25172591882121 22.846070877732924 31.75936563480436 24.142837568641504 41 20.009942697738474 25.295909440644206 28.415035607505857 26.279112254111464 42 20.523941524289345 23.714388102490595 31.25801476297381 24.50365561024625 43 22.718361668265228 22.838868570183852 29.114713435992567 25.328056325558357 44 20.734916435665827 23.175269032536864 30.03081182302701 26.0590027087703 45 21.193053462201938 22.38073154364774 29.407021722862233 27.01919327128809 46 21.93120215296296 25.13412101984675 29.042866026539627 23.891810800650667 47 18.27032192558084 23.621460768503784 33.784443718358155 24.323773587557223 48 20.328249558199914 22.10142254357396 32.25421686323696 25.316111034989163 49 21.202012430128832 21.04953430933384 33.89985630518109 23.848596955356232 50 20.102343033611938 21.237321303723068 32.224529302851764 26.43580635981323 51 19.030780203140207 21.09257248859049 31.125913902561564 28.75073340570774 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 167.0 1 453.5 2 740.0 3 4107.5 4 7475.0 5 4964.0 6 2453.0 7 2446.0 8 2439.0 9 2383.0 10 2327.0 11 2256.0 12 2185.0 13 2156.5 14 2128.0 15 2136.5 16 2145.0 17 2116.0 18 2087.0 19 2013.0 20 1939.0 21 2165.5 22 2392.0 23 2588.5 24 2785.0 25 2925.0 26 3899.5 27 4734.0 28 5239.5 29 5745.0 30 6519.0 31 7293.0 32 8461.5 33 9630.0 34 11604.0 35 13578.0 36 14602.0 37 15626.0 38 17465.5 39 19305.0 40 21115.0 41 22925.0 42 25521.0 43 28117.0 44 31501.5 45 34886.0 46 49275.0 47 63664.0 48 60375.0 49 57086.0 50 56694.5 51 56303.0 52 48864.5 53 41426.0 54 36553.0 55 31680.0 56 29496.5 57 27313.0 58 26614.5 59 25916.0 60 24865.5 61 23815.0 62 21212.5 63 18610.0 64 15161.5 65 11713.0 66 9041.0 67 6369.0 68 4948.0 69 3527.0 70 2835.0 71 2143.0 72 1868.0 73 1593.0 74 1143.0 75 662.0 76 631.0 77 475.0 78 319.0 79 244.5 80 170.0 81 131.0 82 92.0 83 57.5 84 23.0 85 13.0 86 3.0 87 4.0 88 5.0 89 3.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 569262.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.32109458299404 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6066350265057 25.262602826806248 2 9.848619210794583 6.76030782891146 3 3.4061375529009132 3.5070710734740036 4 1.7894030833236627 2.4565708987941037 5 1.1856330996902251 2.034611287759831 6 0.9036813350285753 1.8609199543441237 7 0.7916116906058852 1.9018285794401881 8 0.708100356253465 1.944222344098156 9 0.6535970308562603 2.0188948963663607 >10 7.005209054936473 43.55545716719075 >50 0.06226436371380357 1.4607413894092618 >100 0.031904054134333754 1.8738322018652938 >500 0.0030874891097742345 0.8809307278891692 >1k 0.0036020706280699396 3.58076794465776 >5k 5.145815182957057E-4 0.9012408789932699 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC 5103 0.896423790802829 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG 4715 0.8282653681433154 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC 4507 0.791726832284607 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3987 0.7003804926378363 No Hit GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC 3241 0.5693336284522769 TruSeq Adapter, Index 16 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC 1425 0.25032410383970827 TruSeq Adapter, Index 16 (95% over 21bp) CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT 1228 0.21571789439660474 TruSeq Adapter, Index 13 (95% over 22bp) GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT 1172 0.20588059628079863 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT 960 0.16863939627096133 No Hit GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT 935 0.16424774532640507 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC 880 0.15458611324838123 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTA 877 0.15405911513503448 No Hit TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC 710 0.12472288682539849 TruSeq Adapter, Index 16 (95% over 21bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC 626 0.10996693965168937 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7566603778225142E-4 0.0 0.0 0.2230958679834593 0.0 2 1.7566603778225142E-4 0.0 0.0 1.2753354342991452 0.0 3 1.7566603778225142E-4 0.0 0.0 1.6575847325133242 0.0 4 1.7566603778225142E-4 0.0 0.0 2.3416282836374114 0.0 5 1.7566603778225142E-4 0.0 0.0 4.4577013747624115 0.0 6 1.7566603778225142E-4 0.0 0.0 5.325315935368951 0.0 7 1.7566603778225142E-4 0.0 0.0 6.33715231299472 0.0 8 1.7566603778225142E-4 0.0 0.0 7.595799473704551 0.0 9 1.7566603778225142E-4 0.0 0.0 7.9971963700369955 0.0 10 1.7566603778225142E-4 0.0 0.0 9.762288717673057 0.0 11 1.7566603778225142E-4 0.0 0.0 11.185359289747076 0.0 12 1.7566603778225142E-4 0.0 0.0 13.161075216684058 0.0 13 1.7566603778225142E-4 0.0 0.0 13.659791097947869 0.0 14 1.7566603778225142E-4 0.0 0.0 13.88148163762907 0.0 15 1.7566603778225142E-4 0.0 0.0 14.376332866061674 0.0 16 1.7566603778225142E-4 0.0 0.0 15.117116547389427 0.0 17 1.7566603778225142E-4 0.0 0.0 15.925882985338912 0.0 18 1.7566603778225142E-4 0.0 0.0 16.739041074232954 0.0 19 1.7566603778225142E-4 0.0 0.0 17.52655192161079 0.0 20 1.7566603778225142E-4 0.0 0.0 18.147355699133264 0.0 21 1.7566603778225142E-4 0.0 0.0 18.84229054459985 0.0 22 1.7566603778225142E-4 0.0 0.0 19.594843850459014 0.0 23 1.7566603778225142E-4 0.0 0.0 20.255172486482497 0.0 24 1.7566603778225142E-4 0.0 0.0 20.865260635700256 0.0 25 1.7566603778225142E-4 0.0 0.0 21.38523210753572 0.0 26 1.7566603778225142E-4 0.0 0.0 21.877624011439373 0.0 27 1.7566603778225142E-4 0.0 0.0 22.35894895496274 0.0 28 1.7566603778225142E-4 0.0 0.0 22.8393955682972 0.0 29 1.7566603778225142E-4 0.0 0.0 23.3233555023873 0.0 30 1.7566603778225142E-4 0.0 0.0 23.825233372331194 0.0 31 1.7566603778225142E-4 0.0 0.0 24.346434506431134 0.0 32 1.7566603778225142E-4 0.0 0.0 24.855163351848535 0.0 33 1.7566603778225142E-4 0.0 0.0 25.376364485948475 0.0 34 1.7566603778225142E-4 0.0 0.0 25.862432412491962 0.0 35 1.7566603778225142E-4 0.0 0.0 26.32970407299275 0.0 36 1.7566603778225142E-4 0.0 0.0 26.811380348591687 0.0 37 1.7566603778225142E-4 0.0 0.0 27.277949344941344 0.0 38 1.7566603778225142E-4 0.0 0.0 27.84324265452463 0.0 39 1.7566603778225142E-4 0.0 0.0 28.398522999954327 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCAC 20 7.030281E-4 45.000004 26 CCGTCGA 20 7.030281E-4 45.000004 41 TATTGCG 20 7.030281E-4 45.000004 1 CCATCGC 20 7.030281E-4 45.000004 29 CGGATCA 20 7.030281E-4 45.000004 42 CGTCGAA 20 7.030281E-4 45.000004 42 GCAATCG 20 7.030281E-4 45.000004 21 AATAGCG 20 7.030281E-4 45.000004 1 ATCGGGT 20 7.030281E-4 45.000004 4 CGCAATC 20 7.030281E-4 45.000004 20 GCGCTAG 30 2.1635642E-6 45.000004 1 GTTGCGT 20 7.030281E-4 45.000004 27 TGCGACT 25 3.888317E-5 45.0 21 CGTTTTT 2290 0.0 42.93668 1 CGAGGGT 75 0.0 42.0 4 TCGAAGG 65 0.0 41.53846 2 ACGGCTG 550 0.0 40.909092 8 TACGGCT 555 0.0 40.54054 7 GCGAGAC 45 1.9254003E-8 40.0 21 CACGACC 45 1.9254003E-8 40.0 27 >>END_MODULE