FastQCFastQC Report
Sat 18 Jun 2016
SRR3551714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379103
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC90642.390906956684595No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG80002.110244445440949No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC76112.0076338092813826No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34800.9179563337668127No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC31440.8293260670582928No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT17720.4674191446651701No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT16510.4355016974278758No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA14610.3853833918486533No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT14200.3745683890657684No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC13560.35768643350224083No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC7930.20917798065433404No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT6580.17356760563751802No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4470.11790990838901301No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATG4120.10867758894020886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT302.1616397E-645.0000044
ACGGGTA302.1616397E-645.0000045
TAGTCGG351.2090459E-745.0000042
TTTAGTC207.027299E-445.029
TTAGGCG453.8380676E-1045.01
CGTATTC207.027299E-445.023
CGACGGT207.027299E-445.028
CGTGCCC253.8858438E-545.028
ACACCGC253.8858438E-545.037
CTATACG207.027299E-445.01
CCAATAG207.027299E-445.013
TAAACGG207.027299E-445.02
TCGGGAT502.1827873E-1145.05
CGACCCG253.8858438E-545.032
CGACAAG253.8858438E-545.042
CGTTAGG253.8858438E-545.02
AGACGTG253.8858438E-545.024
CTAACGG207.027299E-445.02
CTACGCG207.027299E-445.01
CTACGAA453.8380676E-1045.011