##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857438
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.321997625484293	31.0	31.0	33.0	28.0	34.0
2	31.287711764582397	31.0	31.0	34.0	28.0	34.0
3	31.132495877252932	31.0	31.0	34.0	28.0	34.0
4	34.930625887819296	35.0	35.0	37.0	32.0	37.0
5	35.29917498408048	35.0	35.0	37.0	33.0	37.0
6	35.25265150366557	37.0	35.0	37.0	33.0	37.0
7	35.55617199144428	37.0	35.0	37.0	33.0	37.0
8	35.16305785374569	37.0	35.0	37.0	32.0	37.0
9	37.07175679174471	39.0	37.0	39.0	33.0	39.0
10	36.5782109027125	38.0	35.0	39.0	32.0	39.0
11	36.83344218474105	39.0	37.0	39.0	33.0	39.0
12	36.98790932988741	39.0	37.0	39.0	33.0	39.0
13	37.043687123733726	39.0	37.0	39.0	33.0	39.0
14	38.06859388084036	40.0	37.0	41.0	33.0	41.0
15	38.150381718561576	40.0	37.0	41.0	33.0	41.0
16	38.19602933389936	40.0	37.0	41.0	34.0	41.0
17	38.00746059773418	40.0	37.0	41.0	33.0	41.0
18	37.957701897979796	39.0	37.0	41.0	33.0	41.0
19	37.882188566403634	39.0	37.0	41.0	33.0	41.0
20	37.83631936070013	39.0	36.0	41.0	33.0	41.0
21	37.686596581910294	39.0	36.0	41.0	33.0	41.0
22	37.862213944331835	39.0	36.0	41.0	33.0	41.0
23	37.88465871584884	40.0	36.0	41.0	33.0	41.0
24	37.74595014450024	39.0	36.0	41.0	33.0	41.0
25	37.31414166388707	39.0	35.0	41.0	32.0	41.0
26	37.45988631247974	39.0	36.0	41.0	33.0	41.0
27	37.56260510964058	39.0	36.0	41.0	33.0	41.0
28	37.3659763154887	39.0	36.0	41.0	32.0	41.0
29	37.50032888675333	39.0	36.0	41.0	33.0	41.0
30	37.21126192214481	39.0	36.0	41.0	32.0	41.0
31	37.17312388767468	39.0	36.0	41.0	32.0	41.0
32	37.101974720038065	39.0	36.0	41.0	32.0	41.0
33	36.94226871214012	39.0	36.0	41.0	31.0	41.0
34	36.87937670128919	39.0	36.0	41.0	31.0	41.0
35	36.774439667941	39.0	36.0	41.0	31.0	41.0
36	36.675100706989895	39.0	35.0	41.0	30.0	41.0
37	36.7497941542129	40.0	36.0	41.0	31.0	41.0
38	36.62320657586904	39.0	35.0	41.0	30.0	41.0
39	36.55786890713964	39.0	35.0	41.0	30.0	41.0
40	36.51779137383694	39.0	35.0	41.0	30.0	41.0
41	36.264194029189284	39.0	35.0	41.0	30.0	41.0
42	36.33948810292989	39.0	35.0	41.0	30.0	41.0
43	36.396601270295925	39.0	35.0	41.0	30.0	41.0
44	36.51839899794504	39.0	35.0	41.0	30.0	41.0
45	36.45943496789272	39.0	35.0	41.0	30.0	41.0
46	36.382728547136935	39.0	35.0	41.0	30.0	41.0
47	36.198723406240454	39.0	35.0	41.0	30.0	41.0
48	36.1775708564351	39.0	35.0	41.0	30.0	41.0
49	36.302639957641254	39.0	35.0	41.0	30.0	41.0
50	36.258780226675285	39.0	35.0	41.0	30.0	41.0
51	35.408198610278525	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	29.0
10	37.0
11	28.0
12	33.0
13	30.0
14	27.0
15	51.0
16	73.0
17	139.0
18	254.0
19	462.0
20	861.0
21	1457.0
22	2467.0
23	3663.0
24	5082.0
25	7179.0
26	9297.0
27	10527.0
28	10732.0
29	11413.0
30	13516.0
31	17141.0
32	22103.0
33	30686.0
34	49403.0
35	58258.0
36	70398.0
37	110264.0
38	204902.0
39	216883.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48299935388914	19.858462069560716	21.794578733389468	16.86395984316067
2	31.88883627737516	28.364849703418788	23.179985025156338	16.566328994049716
3	29.27535285350078	27.786732101912907	27.319992815807094	15.617922228779225
4	27.16348004170564	23.023705504071433	31.84860013202121	17.96421432220172
5	27.876184633757777	26.5717171387319	26.175070384097744	19.377027843412584
6	24.54451517194246	34.47981078515298	26.63411232065759	14.341561722246974
7	71.87680042172146	5.952150476186033	16.933119362566156	5.237929739526356
8	71.72425294890125	8.9086324608893	13.09085904753463	6.2762555426748055
9	65.56963885435448	8.043030516492154	14.80223642992263	11.585094199230731
10	35.25362766753981	28.468647295781153	21.794462106881195	14.483262929797839
11	26.096697370538745	23.727313228478327	30.969352886156198	19.206636514826727
12	23.354341655023454	21.381954147122006	33.902509569205	21.361194628649535
13	22.788353210377892	22.499002843354273	36.37767395426842	18.334969991999422
14	19.152055308955283	27.619256436033858	31.64520350159428	21.583484753416574
15	17.018606593129764	25.838253028207287	37.735323137066466	19.407817241596476
16	19.62485917349126	26.4246511118005	31.57301169297372	22.377478021734515
17	19.418780133374074	26.603439548981967	32.31312351446985	21.664656803174108
18	19.047557957543283	26.15221158847637	32.66300303928681	22.13722741469354
19	20.176852437144145	27.104467028519846	30.33140588590662	22.387274648429393
20	21.878316566328994	27.34844968382554	32.97509557542353	17.79813817442194
21	21.143103058180298	28.737821276873664	31.46081699201575	18.658258672930288
22	19.781138694576168	24.329805770213124	31.97537314651322	23.91368238869749
23	20.12833580970286	27.45236390269617	32.101912907988684	20.31738737961229
24	20.978309801991514	24.771237104023847	30.917220836958474	23.333232257026165
25	18.924866870840805	29.93697503492964	29.067174536234692	22.070983557994865
26	18.77208614500407	26.268138337699053	31.627243019320346	23.33253249797653
27	21.58686692215647	26.451941714736225	30.27414227034491	21.687049092762393
28	17.180134307086924	26.308374483053004	32.59979147180321	23.911699738056864
29	19.60468278756015	23.46023852453472	31.644970248577742	25.29010843932739
30	18.95518976299161	25.53397446812481	33.0523023238998	22.45853344498378
31	19.5623473650573	23.983308414136065	29.769032863017504	26.685311357789132
32	19.774490983604647	25.467613984917858	30.95010951229127	23.80778551918623
33	18.400747342665007	24.668022644202846	29.71316876555506	27.218061247577086
34	18.705375782272306	23.554239490202207	31.528810246338512	26.21157448118698
35	18.257063484473512	23.43516382525617	30.700528784588503	27.607243905681813
36	17.262006115894092	26.90142027761774	28.92524007566728	26.911333530820887
37	17.385513588154478	24.668955656269027	35.13980019546602	22.80573056011047
38	17.96164853901973	25.210452534177396	28.402403439082473	28.425495487720394
39	20.17976809985095	23.8622500985494	32.294113393621465	23.663868407978185
40	19.42659410942832	22.675575376878562	31.96277748361981	25.935053030073313
41	17.90683408013174	26.4052911114273	30.302249258838536	25.38562554960242
42	19.091292898145408	25.08542891730947	31.13193023868781	24.69134794585731
43	20.596124734383128	25.566863143457603	29.5082559905206	24.328756131638674
44	20.003195566326664	23.60217298510213	30.692831435042535	25.701800013528675
45	19.56607941332201	21.848110300686464	29.982109493631025	28.6037007923605
46	22.24615657341989	24.487718062413844	29.274536467942873	23.9915888962234
47	17.4519906978697	23.647657323328332	34.62372789636102	24.276624082440946
48	19.025165667955	24.091537813812778	30.410595285023522	26.4727012332087
49	19.209085671500446	21.959488616086528	34.37543006024926	24.45599565216377
50	19.19765627368976	21.998091990324664	32.33796496073185	26.46628677525372
51	18.468157464446406	21.603544512839413	29.697657439954845	30.23064058275934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185.0
1	548.5
2	912.0
3	6026.0
4	11140.0
5	7546.0
6	3952.0
7	3917.5
8	3883.0
9	3761.5
10	3640.0
11	3679.0
12	3718.0
13	3534.0
14	3350.0
15	3159.0
16	2968.0
17	2934.5
18	2901.0
19	2956.0
20	3011.0
21	3920.0
22	4829.0
23	4745.5
24	4662.0
25	5696.0
26	7546.5
27	8363.0
28	10580.5
29	12798.0
30	14241.0
31	15684.0
32	17506.5
33	19329.0
34	21704.0
35	24079.0
36	26368.5
37	28658.0
38	30896.0
39	33134.0
40	35989.0
41	38844.0
42	42416.5
43	45989.0
44	50594.0
45	55199.0
46	83572.0
47	111945.0
48	101190.5
49	90436.0
50	86793.0
51	83150.0
52	69750.0
53	56350.0
54	48330.0
55	40310.0
56	35970.0
57	31630.0
58	29211.5
59	26793.0
60	24793.0
61	22793.0
62	20495.0
63	18197.0
64	15255.0
65	12313.0
66	10095.0
67	7877.0
68	6750.5
69	5624.0
70	4899.0
71	4174.0
72	3581.0
73	2988.0
74	2598.0
75	1720.0
76	1232.0
77	1059.0
78	886.0
79	596.0
80	306.0
81	226.0
82	146.0
83	107.5
84	69.0
85	53.5
86	38.0
87	22.0
88	6.0
89	7.0
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	857438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.775734700959354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.25803759726735	24.743440425982076
2	10.383809475557662	7.0144158806348695
3	3.675187659781723	3.723964901190814
4	1.8272896027787546	2.4687219538110647
5	1.1713053073104955	1.9780848656772483
6	0.8677342760658843	1.7585017619598198
7	0.7004466958140337	1.6560671238984865
8	0.6399908237758434	1.7292928219921053
9	0.5835931964949762	1.7740160080289242
>10	6.771241768408275	40.92077859488833
>50	0.06746286495978496	1.5636651905711971
>100	0.04625030533837832	2.8679914836607496
>500	0.002434226596756405	0.5042299713061923
>1k	0.003825213223474351	2.823358968147741
>5k	3.4774665667948646E-4	0.7748439600994528
>10k+	0.0010432399700384593	3.698626088150943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	11172	1.3029513504183392	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	10248	1.195188456774717	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	10070	1.1744289383022446	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6597	0.7693850750724833	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	4877	0.568787480844096	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	2634	0.30719422278928626	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	2582	0.30112964435912565	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	2283	0.2662583183857025	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	2181	0.2543624145419261	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	2091	0.2438660287974174	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTCTCAT	1945	0.22683855858965896	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTA	1932	0.22532241398211883	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	1317	0.15359711139464313	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1128	0.13155470133117497	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1068	0.12455711083483587	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3325301654463645E-4	0.0	0.0	0.2500472337358503	0.0
2	2.3325301654463645E-4	0.0	0.0	1.335840025751133	0.0
3	2.3325301654463645E-4	0.0	0.0	1.7632761785691793	0.0
4	2.3325301654463645E-4	0.0	0.0	2.668764388795458	0.0
5	2.3325301654463645E-4	0.0	0.0	5.4784135995838765	0.0
6	2.3325301654463645E-4	0.0	0.0	6.55417651188774	0.0
7	2.3325301654463645E-4	0.0	0.0	7.796365451496201	0.0
8	2.3325301654463645E-4	0.0	0.0	9.453628134045843	0.0
9	2.3325301654463645E-4	0.0	0.0	9.989177060032329	0.0
10	2.3325301654463645E-4	0.0	0.0	12.218026259624603	0.0
11	2.3325301654463645E-4	0.0	0.0	13.74630002402506	0.0
12	2.3325301654463645E-4	0.0	0.0	16.248405132499375	0.0
13	2.3325301654463645E-4	0.0	0.0	16.847748758510818	0.0
14	2.3325301654463645E-4	0.0	0.0	17.10875888402427	0.0
15	2.3325301654463645E-4	0.0	0.0	17.712301064333513	0.0
16	2.3325301654463645E-4	0.0	0.0	18.45474541599509	0.0
17	2.3325301654463645E-4	0.0	0.0	19.356851457481476	0.0
18	2.3325301654463645E-4	0.0	0.0	20.261523282149845	0.0
19	2.3325301654463645E-4	0.0	0.0	21.153832696941354	0.0
20	2.3325301654463645E-4	0.0	0.0	21.804841866117435	0.0
21	2.3325301654463645E-4	0.0	0.0	22.548452482861734	0.0
22	2.3325301654463645E-4	0.0	0.0	23.415687198374695	0.0
23	6.997590496339094E-4	0.0	0.0	24.180057333591467	0.0
24	6.997590496339094E-4	0.0	0.0	24.79549541774449	0.0
25	6.997590496339094E-4	0.0	0.0	25.32486313879254	0.0
26	6.997590496339094E-4	0.0	0.0	25.821692064032618	0.0
27	6.997590496339094E-4	0.0	0.0	26.356307977952923	0.0
28	6.997590496339094E-4	0.0	0.0	26.88485931344307	0.0
29	6.997590496339094E-4	0.0	0.0	27.421341251495736	0.0
30	6.997590496339094E-4	0.0	0.0	28.021034757031995	0.0
31	6.997590496339094E-4	0.0	0.0	28.57617693640823	0.0
32	6.997590496339094E-4	0.0	0.0	29.10846032016309	0.0
33	6.997590496339094E-4	0.0	0.0	29.634795751996062	0.0
34	6.997590496339094E-4	0.0	0.0	30.155999617465053	0.0
35	8.163855579062276E-4	0.0	0.0	30.70029553157196	0.0
36	8.163855579062276E-4	0.0	0.0	31.2128690354288	0.0
37	8.163855579062276E-4	0.0	0.0	31.75506567238681	0.0
38	8.163855579062276E-4	0.0	0.0	32.279418453579154	0.0
39	8.163855579062276E-4	0.0	0.0	32.802371716672226	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	30	2.1648539E-6	45.000004	25
CACGTAG	30	2.1648539E-6	45.000004	1
GTTTACG	20	7.0322823E-4	45.0	1
ATATGCG	20	7.0322823E-4	45.0	1
CAGTACG	20	7.0322823E-4	45.0	1
TGTCGCG	20	7.0322823E-4	45.0	2
CGTTTTT	3860	0.0	43.076424	1
TACGGCT	1160	0.0	40.926723	7
TGCGTAG	50	1.0804797E-9	40.5	1
AAATGCG	50	1.0804797E-9	40.5	1
TTTAGCG	45	1.9274012E-8	40.0	1
CGATCTC	45	1.9274012E-8	40.0	34
CTCGTCC	40	3.4568438E-7	39.375	37
GCGATAT	40	3.4568438E-7	39.375	9
TATACGG	40	3.4568438E-7	39.375	2
CGTATGG	75	0.0	39.000004	2
ACGGCTG	1235	0.0	38.805664	8
TCGTTAG	35	6.2464333E-6	38.571426	1
CCGATGA	660	0.0	38.18182	18
TGATACC	1380	0.0	37.826088	4
>>END_MODULE