##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551708_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167195
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41745865606029	31.0	31.0	33.0	28.0	34.0
2	31.37095008822034	31.0	31.0	34.0	28.0	34.0
3	31.237261879840904	31.0	31.0	34.0	28.0	34.0
4	34.98818146475672	35.0	35.0	37.0	32.0	37.0
5	35.370064894285115	37.0	35.0	37.0	33.0	37.0
6	35.318657854600914	37.0	35.0	37.0	33.0	37.0
7	35.55709201830198	37.0	35.0	37.0	33.0	37.0
8	35.13481862495888	37.0	35.0	37.0	32.0	37.0
9	37.0778252938186	39.0	37.0	39.0	33.0	39.0
10	36.69582822452825	38.0	35.0	39.0	32.0	39.0
11	36.93739645324322	39.0	37.0	39.0	33.0	39.0
12	36.94236669756871	39.0	37.0	39.0	33.0	39.0
13	36.94577589042734	39.0	37.0	39.0	33.0	39.0
14	37.8886569574449	40.0	37.0	41.0	33.0	41.0
15	38.14763001285924	40.0	37.0	41.0	33.0	41.0
16	38.09583420556835	40.0	37.0	41.0	34.0	41.0
17	38.047441610095994	40.0	37.0	41.0	34.0	41.0
18	37.980968330392656	39.0	37.0	41.0	34.0	41.0
19	37.85441550285595	39.0	37.0	41.0	34.0	41.0
20	37.86031280839738	39.0	36.0	41.0	34.0	41.0
21	37.57884506115614	39.0	35.0	41.0	33.0	41.0
22	37.75866503184904	39.0	35.0	41.0	33.0	41.0
23	37.84684350608571	39.0	35.0	41.0	34.0	41.0
24	37.74036903017435	39.0	35.0	41.0	34.0	41.0
25	37.28771195310865	39.0	35.0	41.0	33.0	41.0
26	37.44614372439367	39.0	35.0	41.0	33.0	41.0
27	37.615574628427886	39.0	35.0	41.0	33.0	41.0
28	37.533933431023655	39.0	35.0	41.0	33.0	41.0
29	37.566362630461434	39.0	35.0	41.0	33.0	41.0
30	37.15867699392924	39.0	35.0	41.0	33.0	41.0
31	37.1496396423338	39.0	35.0	41.0	33.0	41.0
32	37.05658662041329	39.0	35.0	41.0	33.0	41.0
33	37.011836478363584	39.0	35.0	41.0	32.0	41.0
34	36.899458715870686	39.0	35.0	41.0	32.0	41.0
35	36.806603068273574	39.0	35.0	41.0	32.0	41.0
36	36.58983223182511	39.0	35.0	41.0	31.0	41.0
37	36.63970812524298	39.0	35.0	41.0	31.0	41.0
38	36.443147223302134	39.0	35.0	41.0	31.0	41.0
39	36.42181883429529	39.0	35.0	41.0	31.0	41.0
40	36.33578157241544	39.0	35.0	41.0	30.0	41.0
41	36.20907323783606	39.0	35.0	41.0	30.0	41.0
42	36.28374652352044	39.0	35.0	41.0	30.0	41.0
43	36.28789736535183	39.0	35.0	41.0	30.0	41.0
44	36.27965549209007	39.0	35.0	41.0	30.0	41.0
45	36.24410418971859	39.0	35.0	41.0	30.0	41.0
46	36.16796554920901	39.0	35.0	41.0	30.0	41.0
47	36.04835670923173	39.0	35.0	41.0	30.0	41.0
48	35.96582433685218	39.0	35.0	41.0	29.0	41.0
49	36.075785759143514	39.0	35.0	41.0	30.0	41.0
50	36.03848201202189	39.0	35.0	41.0	30.0	41.0
51	35.223050928556475	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	11.0
10	13.0
11	10.0
12	14.0
13	6.0
14	10.0
15	17.0
16	11.0
17	30.0
18	53.0
19	99.0
20	152.0
21	245.0
22	390.0
23	569.0
24	864.0
25	1383.0
26	1991.0
27	2250.0
28	2193.0
29	2216.0
30	2429.0
31	3066.0
32	4129.0
33	6155.0
34	11684.0
35	13330.0
36	12728.0
37	20410.0
38	38256.0
39	42469.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.8181165704716	22.721971350817906	23.820090313705553	15.639821765004935
2	35.945452914261786	24.11136696671551	23.42055683483358	16.52262328418912
3	32.75576422739914	21.503633481862497	28.543915786955353	17.196686503783006
4	29.564879332515925	24.326086306408683	27.423666975687073	18.685367385388318
5	25.295014803074256	28.170698884536023	26.557014264780644	19.97727204760908
6	25.37157211639104	32.39092078112384	26.99961123239331	15.237895870091808
7	72.11459672837105	8.404557552558389	14.067406321959389	5.413439397111158
8	71.5912557193696	7.064206465504351	14.650557731989593	6.693980083136457
9	64.94392774903555	9.494303059302013	17.48497263674153	8.0767965549209
10	38.29301115463979	22.454618858219444	24.333263554532135	14.919106432608631
11	29.637848021770985	24.02045515715183	28.089356739136935	18.25234008194025
12	25.80878614791112	23.12688776578247	32.5727444002512	18.491581686055206
13	22.49050509883669	26.391937557941326	33.33293459732648	17.78462274589551
14	18.244564729806516	29.38963485750172	32.21328389006848	20.152516522623284
15	18.072908878854033	26.339902509046325	37.69909387242441	17.88809473967523
16	18.952121773976494	26.27171865187356	34.380812823349984	20.395346750799963
17	19.949161159125573	25.903884685546817	30.2867908729328	23.860163282394808
18	19.873201949819077	26.409880678249948	33.83115523789587	19.885762134035108
19	22.058075899398904	25.97864768683274	31.544005502556892	20.41927091121146
20	22.626274709171927	27.129399802625677	32.04581476718801	18.198510721014387
21	21.943838033434016	27.497831872962706	32.01172283860163	18.546607255001646
22	20.194383803343403	25.678399473668474	31.072101438440146	23.055115284547984
23	19.948563055115283	26.38655462184874	32.547025927808846	21.11785639522713
24	20.81043093393941	25.395496276802536	33.50040372020695	20.293669069051106
25	19.170429737731393	27.436825263913395	31.417207452375965	21.975537545979247
26	17.577080654325787	29.57325278865995	31.447710756900626	21.40195580011364
27	20.989264033015342	28.625257932354437	31.356200843326654	19.029277191303567
28	17.584257902449234	28.194623044947516	32.80959358832501	21.411525464278238
29	19.132749185083288	28.4847034899369	31.28143784204073	21.101109482939084
30	20.595113490235953	30.15161936660785	29.682705822542538	19.570561320613656
31	24.964861389395615	27.53371811357995	27.548670713837137	19.952749783187297
32	23.221388199407876	28.977541194413707	26.935015999282275	20.86605460689614
33	22.24468435060857	28.316038159035855	28.446424833278506	20.992852657077066
34	21.45877568109094	26.306408684470227	29.250874727115043	22.98394090732378
35	21.45399084900864	26.987649152187565	28.38063339214689	23.177726606656897
36	22.040730883100572	29.26463111935165	28.521187834564433	20.173450162983343
37	22.749484135291127	28.33099075929304	28.804091031430367	20.115434073985465
38	21.256616525613804	28.87885403271629	27.516971201291906	22.347558240378003
39	22.691468046293252	26.665869194652952	29.480546667065404	21.1621160919884
40	22.525195131433357	26.229851371153444	29.886659290050538	21.35829420736266
41	19.64532432189958	26.16346182601154	28.532551810759895	25.658662041328988
42	20.042465384730406	27.092915457998146	30.015849756272615	22.848769400998833
43	22.739316367116242	24.893687012171416	29.972188163521636	22.394808457190702
44	21.154938843864947	26.76874308442238	28.88064834474715	23.19566972696552
45	22.31107389575047	26.896737342623883	26.683812314961568	24.108376446664074
46	22.11190526032477	27.30045754956787	28.67729298124944	21.91034420885792
47	20.802057477795387	25.071323903226773	32.11818535243279	22.00843326654505
48	20.06220281706989	25.162833816800738	31.711474625437365	23.063488740692005
49	21.915727144950505	23.625108406351863	33.17862376267232	21.2805406860253
50	20.22249469182691	24.66102455216962	31.731810161787134	23.384670594216335
51	19.850473997428153	24.355393402912767	28.512216274410118	27.28191632524896
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	231.5
2	341.0
3	1727.5
4	3114.0
5	2142.5
6	1171.0
7	1107.0
8	1043.0
9	1003.5
10	964.0
11	897.5
12	831.0
13	782.0
14	733.0
15	731.5
16	730.0
17	728.5
18	727.0
19	694.5
20	662.0
21	892.5
22	1123.0
23	1042.5
24	962.0
25	1157.5
26	1618.0
27	1883.0
28	2196.0
29	2509.0
30	2844.0
31	3179.0
32	3681.0
33	4183.0
34	4278.5
35	4374.0
36	4375.0
37	4376.0
38	5137.0
39	5898.0
40	6876.5
41	7855.0
42	9135.5
43	10416.0
44	10955.0
45	11494.0
46	12985.0
47	14476.0
48	16423.0
49	18370.0
50	17492.5
51	16615.0
52	14075.0
53	11535.0
54	10190.0
55	8845.0
56	7630.0
57	6415.0
58	5652.0
59	4889.0
60	4634.5
61	4380.0
62	4003.5
63	3627.0
64	3243.0
65	2859.0
66	2424.5
67	1990.0
68	1589.5
69	1189.0
70	1011.0
71	833.0
72	693.5
73	554.0
74	412.0
75	230.0
76	190.0
77	136.5
78	83.0
79	53.0
80	23.0
81	15.0
82	7.0
83	3.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.52202518017884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17421351718717	27.08992493794671
2	9.897974223343105	7.229881276353958
3	3.4145063295285194	3.741140584347618
4	1.8227076953310515	2.662759053799456
5	1.1365311235936655	2.0754209156972396
6	0.9105350212076053	1.9952749783187296
7	0.7418567708759806	1.8965878166213104
8	0.6698000425789759	1.9569963216603365
9	0.5518890326384226	1.8140494632016508
>10	6.532597481289816	42.323035975956216
>50	0.10153448078214303	2.454020754209157
>100	0.0393036699801844	2.4946918269086993
>500	0.00491295874752305	1.1094829390831067
>1k	0.0016376529158410168	1.1567331558958103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1934	1.1567331558958103	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	752	0.4497742157361165	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	572	0.3421154938843865	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	531	0.31759322946260354	No Hit
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	333	0.1991686354257005	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	303	0.18122551511707885	No Hit
ACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	298	0.17823499506564192	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	265	0.1584975627261581	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	248	0.14832979455127246	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCC	242	0.14474117048954815	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	232	0.13876013038667423	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	172	0.1028738897694309	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32955530966835134	0.0
2	0.0	0.0	0.0	1.532940578366578	0.0
3	0.0	0.0	0.0	2.1029337001704596	0.0
4	0.0	0.0	0.0	2.9384850025419422	0.0
5	0.0	0.0	0.0	4.854212147492449	0.0
6	0.0	0.0	0.0	5.832710308322618	0.0
7	0.0	0.0	0.0	6.763360148329794	0.0
8	0.0	0.0	0.0	8.191034420885792	0.0
9	0.0	0.0	0.0	8.722150782020993	0.0
10	0.0	0.0	0.0	9.800532312569155	0.0
11	0.0	0.0	0.0	11.542809294536319	0.0
12	0.0	0.0	0.0	12.849068453003978	0.0
13	0.0	0.0	0.0	13.420855886838721	0.0
14	0.0	0.0	0.0	13.663686115015402	0.0
15	0.0	0.0	0.0	14.110469810700081	0.0
16	0.0	0.0	0.0	14.986692185771105	0.0
17	0.0	0.0	0.0	15.994497443105356	0.0
18	0.0	0.0	0.0	17.13209127067197	0.0
19	0.0	0.0	0.0	17.748736505278266	0.0
20	0.0	0.0	0.0	18.380932444152037	0.0
21	0.0	0.0	0.0	19.084900864260295	0.0
22	0.0	0.0	0.0	19.857053141541314	0.0
23	0.0	0.0	0.0	20.59571159424624	0.0
24	0.0	0.0	0.0	21.247046861449206	0.0
25	0.0	0.0	0.0	21.771584078471246	0.0
26	0.0	0.0	0.0	22.243488142587996	0.0
27	0.0	0.0	0.0	22.743503095188252	0.0
28	0.0	0.0	0.0	23.24351804778851	0.0
29	0.0	0.0	0.0	23.70585244774066	0.0
30	0.0	0.0	0.0	24.29079816980173	0.0
31	0.0	0.0	0.0	24.78722449834026	0.0
32	0.0	0.0	0.0	25.286043242919945	0.0
33	0.0	0.0	0.0	25.80220700379796	0.0
34	0.0	0.0	0.0	26.286073148120458	0.0
35	0.0	0.0	0.0	26.841113669667156	0.0
36	0.0	0.0	0.0	27.328568438051377	0.0
37	0.0	0.0	0.0	27.809444062322438	0.0
38	0.0	0.0	0.0	28.26280690212028	0.0
39	0.0	0.0	0.0	28.808875863512664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCA	20	7.01598E-4	45.0	35
AAGTAGG	50	2.1827873E-11	45.0	2
CTCAACG	20	7.01598E-4	45.0	1
GGTAACG	20	7.01598E-4	45.0	34
TTAGCAT	20	7.01598E-4	45.0	36
GTCCTCT	20	7.01598E-4	45.0	8
CGGCAGA	20	7.01598E-4	45.0	16
GACCGAG	20	7.01598E-4	45.0	9
TCGGGTA	20	7.01598E-4	45.0	27
ATAGCGG	25	3.8764658E-5	45.0	2
GCGTTTT	20	7.01598E-4	45.0	9
TAACGGT	20	7.01598E-4	45.0	36
CGACAGG	40	6.7557266E-9	45.0	2
CGCTGGT	20	7.01598E-4	45.0	12
ACGGGCC	35	1.2036071E-7	45.0	5
CGTTAGG	25	3.8764658E-5	45.0	2
ATTCCTC	20	7.01598E-4	45.0	11
AACGGTA	20	7.01598E-4	45.0	37
AATGCGG	20	7.01598E-4	45.0	2
CTAAAGG	25	3.8764658E-5	45.0	2
>>END_MODULE