FastQCFastQC Report
Sat 18 Jun 2016
SRR3551704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863033
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56580.6555948613784178No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC49190.5699666177307241No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC36220.4196826772556785No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG32740.37935976955689993No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC25430.2946584893045805No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT14190.16442013225450244No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT12290.1424047516143647No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT11910.13800167548633716TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC9370.10857058768320561No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG253.8899958E-545.01
GTATACG253.8899958E-545.01
CGGTCTA406.8121153E-945.031
GACTACG207.0323097E-445.01
TCGAACG302.164872E-644.9999961
CGTTTTT37100.042.998651
CTACGAA700.041.78571711
TATAGCG603.6379788E-1241.2499961
ACACGAC1800.040.026
CACGACC1800.040.027
TGATGCG451.9274012E-840.01
AACACGT1950.039.2307741
CGAATAT750.039.014
TCGCGCG356.2464787E-638.571431
GCGAGAC2000.038.2521
TCAAGCG2000.038.2517
GCGTAAG900.037.51
GCCGAAT301.1396864E-437.49999617
CGTTTAC301.1396864E-437.49999633
CGCCCCG301.1396864E-437.49999625